The intracellular development of vaccinia virus as observed in freeze-etched preparations

1971 ◽  
Vol 17 (6) ◽  
pp. 753-757 ◽  
Author(s):  
K. B. Easterbrook ◽  
K. R. Rozee
Keyword(s):  
Protein Structure ◽  
Granular Material ◽  
Vaccinia Virus ◽  
Condensed State ◽  
L Cells ◽  
Fibrous Protein ◽  
Immature Virion ◽  
Viral Inclusion

The site and structure of the viral inclusion can be easily seen in freeze-etched preparations of vaccinia-infected L-cells. It can be recognized not only by its content of virions but also by its granular nature which contrasts with the almost structureless cytoplasm. The granular material (viroplasm) appears to be incorporated into the developing immature virion in a condensed state. The inclusion is surrounded by ribosomes and a fibrous protein structure which appears to contain it, and is interspersed with membrane-bounded vesicles and vacuoles. Both the internal and external surfaces of the virions are visualized and appear similar to those observed in negatively stained preparations of isolated virions.

Processing and Characterization of Recombinant von Willebrand Factor Expressed in Different Cell Types Using a Vaccinia Virus Vector

1992 ◽  
Vol 67 (01) ◽  
pp. 154-160 ◽  
Author(s):  
P Meulien ◽  
M Nishino ◽  
C Mazurier ◽  
K Dott ◽  
G Piétu ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Von Willebrand Factor ◽  
Human Fibroblasts ◽  
Active Form ◽  
Cell Types ◽  
Biologically Active ◽  
L Cells ◽  
Von Willebrand ◽  
Different Cell Types ◽  
Willebrand Factor

SummaryThe cloning of the cDNA encoding von Willebrand factor (vWF) has revealed that it is synthesized as a large precursor (pre-pro-vWF) molecule and it is now clear that the prosequence or vWAgll is responsible for the intracellular multimerization of vWF. We have cloned the complete vWF cDNA and expressed it using a recombinant vaccinia virus as vector. We have characterized the structure and function of the recombinant vWF (rvWF) secreted from five different cell types: baby hamster kidney (BHK), Chinese hamster ovary (CHO), human fibroblasts (143B), mouse fibroblasts (L) and primary embryonic chicken cells. Forty-eight hours after infection, the quantity of vWF antigen found in the cell supernatant varied from 3 to 12 U/dl depending on the cell type. By SDS-agarose gel electrophoresis, the percentage of high molecular weight forms of vWF varied from 39 to 49% relative to normal plasma for BHK, CHO, 143B and chicken cells but was less than 10% for L cells. In all cell types, the two anodic subbands of each multimer were missing. The two cathodic subbands were easily detected only in BHK and L cells. By SDS-PAGE of reduced samples, pro-vWF was present in similar quantity to the fully processed vWF subunit in L cells, present in moderate amounts in BHK and CHO and in very low amounts in 143B and chicken cells. rvWF from all cells bound to collagen and to platelets in the presence of ristocetin, the latter showing a high correlation between binding efficiency and degree of multimerization. rvWF from all cells was also shown to bind to purified FVIII and in this case binding appeared to be independent of the degree of multimerization. We conclude that whereas vWF is naturally synthesized only by endothelial cells and megakaryocytes, it can be expressed in a biologically active form from various other cell types.

The Influence of Hydrocortisone on Vaccinia Virus Grown in L Cells

1962 ◽  
Vol 110 (3) ◽  
pp. 268-277 ◽  
Author(s):  
M. Holden ◽  
L. B. Adams
Keyword(s):  
Vaccinia Virus ◽  
L Cells

scholarly journals The Mode of Entry of Vaccinia Virus into L Cells

1973 ◽  
Vol 21 (3) ◽  
pp. 533-537 ◽  
Author(s):  
J. A. Armstrong ◽  
D. H. Metz ◽  
M. R. Young
Keyword(s):  
Vaccinia Virus ◽  
Mode Of Entry ◽  
L Cells

Replication of vaccinia virus DNA in enucleated L-cells

1971 ◽  
Vol 59 (3) ◽  
pp. 505-508 ◽  
Author(s):  
D.M. Prescott ◽  
J. Kates ◽  
J.B. Kirkpatrick
Keyword(s):  
Vaccinia Virus ◽  
L Cells

The cycle of multiplication of vaccinia virus in Earle's strain L cells I. Uptake and penetration

Virology ◽  
1964 ◽  
Vol 24 (3) ◽  
pp. 278-294 ◽  
Author(s):  
Samuel Dales ◽  
Robert Kajioka
Keyword(s):  
Vaccinia Virus ◽  
L Cells
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