The effect of gibberellic acid on Hansenula wingei

1969 ◽  
Vol 15 (10) ◽  
pp. 1225-1230 ◽  
Author(s):  
Emile H. Makarem ◽  
Norman Alldridge
Keyword(s):  
Protein Synthesis ◽  
Cell Division ◽  
Gibberellic Acid ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Rna And Protein Synthesis ◽  
Hansenula Wingei

The present study shows the influence of gibberellic acid on the budding and cell division of three strains of Hansenula wingei. Gibberellic acid at 10 μg/ml increased cell division of all the strains of H. wingei tested. The rate of budding and cell division diminished as the concentration of gibberellic acid increased over or decreased below 10 μg/ml. Also tested was the ability of gibberellic acid at a concentration of 10 μg/ml to stimulate the synthesis of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and protein contents of both haploid and diploid strains of H. wingei. The rate of DNA, RNA, and protein synthesis decreased as the concentration of gibberellic acid was increased over or decreased below 10 μg/ml.

Regulation of K+ Influx in Barley Roots: Evidence for Direct Control by Internal K+

1977 ◽  
Vol 4 (2) ◽  
pp. 313 ◽  
Author(s):  
ADM Glass
Keyword(s):  
Protein Synthesis ◽  
Cell Division ◽  
Actinomycin D ◽  
Direct Control ◽  
Pretreatment Period ◽  
Rna And Protein Synthesis ◽  
Lag Period ◽  
Limited Protein ◽  
K Concentration ◽  
Gamma Irradiated

Values for plasmalemma influx of K+ into excised barley roots, from solutions containing 0.05 mM KCl plus 0.5 mM CaSO4, were reduced by 50-60% following a 6-h pretreatment period in 50 mM KCl plus 0.05 mM CaSO4 solution. This reduction of influx, associated with increased internal K+ concentration, was independent of DNA, RNA and protein synthesis during the pretreatment period as indicated by its insensitivity to the presence of 5-fluorodeoxyuridine, actinomycin D, cycloheximide, p-fluorophenylalanine or anisomycin in the pretreatment solutions. Roots of plantlets grown from gamma-irradiated barley seeds, which were incapable of under-going cell division and capable of only limited protein synthesis, were nevertheless able to reduce K+ influx values in response to increased internal K+ concentration. The measurement of K+ influx from 0.05 mM KCl solutions following pretreatment periods as short as 15 min in 50 mM KCl gave no evidence of any lag period in the development of reduced influx associated with increased internal K+ status. The above experiments are discussed in terms of a model for the regulation of K+ influx which ascribes a direct 'allosteric' role to internal K+ in controlling influx.

EFFECT OF OESTRADIOL-17β ON CLEAVAGE, NUCLEIC ACID METABOLISM AND PROTEIN SYNTHESIS IN SEA URCHIN, STRONGYLOCENTROTUS PURPURATUS, EMBRYOS

1962 ◽  
Vol 25 (2) ◽  
pp. 183-NP ◽  
Author(s):  
W. B. JOLLEY ◽  
W. E. MARTIN ◽  
J. W. BAMBERGER ◽  
L. W. STEARNS
Keyword(s):  
Protein Synthesis ◽  
Nucleic Acid ◽  
Sea Urchin ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Acid Metabolism ◽  
Strongylocentrotus Purpuratus ◽  
Nucleic Acid Metabolism ◽  
Dna And Rna ◽  
Moderate Effect

SUMMARY Oestradiol-17β at a concentration of 3 × 10−3 m inhibits cleavage in sea urchin (Strongylocentrotus purpuratus) embryos. This inhibition is accompanied by a reduction in deoxyribonucleic acid (DNA) synthesis and little change in ribonucleic acid (RNA). The effects of oestradiol-17β upon the incorporation of glycine-1-14C and glycine-2-14C into the purines of DNA and RNA and the incorporation of glycine-2-14C into serine were studied. The incorporation of glycine-1-14C and glycine-2-14C into RNA was reduced, but the incorporation of glycine-2-14C into DNA was increased considerably over that of the controls. The incorporation of glycine-2-14C into serine was also accelerated by oestradiol. A possible explanation of the action of oestradiol-17β is offered. The moderate effect upon RNA is not surprising because there is little or no synthesis of this compound from the time of fertilization to blastulation under normal conditions.

scholarly journals Alterations in Nuclear Ribonucleic Acid Metabolism Induced by Kinetin

1957 ◽  
Vol 3 (1) ◽  
pp. 129-132 ◽  
Author(s):  
Ruth Guttman
Keyword(s):  
Cell Division ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Acid Metabolism ◽  
Root Cells ◽  
Onion Root ◽  
Azure B ◽  
Division Factor

Removal of deoxyribonucleic acid from meristematic onion root cells grown in solutions of kinetin, followed by metachromatic staining in azure B bromide, indicated the presence of appreciable amounts of ribonucleic acid in nuclei exposed to the cell division factor.

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