Preferential utilization of the methane component of natural gas by a mixed culture of bacteria

1969 ◽  
Vol 15 (9) ◽  
pp. 1114-1116 ◽  
Author(s):  
J. C. Mueller
Keyword(s):  
Carbon Source ◽  
Natural Gas ◽  
Mixed Culture ◽  
Selective Enrichment ◽  
Preferential Utilization

A mixed culture of bacteria was grown on natural gas for 6 months. When tested at this time, the culture was found to use methane as the preferred carbon source. No selective enrichment of ethane or propane utilizers was observed.

Growth of Graphium sp. on natural gas

1969 ◽  
Vol 15 (10) ◽  
pp. 1231-1236 ◽  
Author(s):  
J. E. Zajic ◽  
B. Volesky ◽  
Angela Wellman
Keyword(s):  
Carbon Source ◽  
Natural Gas ◽  
Continuous Culture ◽  
Mixed Culture ◽  
Continuous Cultivation ◽  
Dry Weight ◽  
Mineral Salts ◽  
Ambient Temperatures ◽  
Acid Tolerant ◽  
Enrichment Techniques

A fungus which grows well on a mineral salts solution with natural gas as the carbon source is described and provisionally identified as a Graphium species. Its taxonomic relation to several genera is presented. This organism was isolated from sewage after selection by enrichment techniques and continuous culture. The fermentor was operated at ambient temperatures, 28 °C ± 2, at a volume of 10 liters with a dilution rate of 10 liters/4 days to 10 liters/1.7 days. Coty's mineral salts medium gave the highest tissue yield. When the pH of the incoming mineral salts medium was decreased stepwise from 7.0 to 5.0 the pH of the reactor became self-adjusting, varying from around 2.7 to 3.5, and the dry weight of microbial tissue obtained varied from 65 to 275 mg/h. Also present in the continuous culture was an acid tolerant bacterium, which, when isolated, grew well on natural gas, methanol, and ethanol, and a strain of Trichoderma, which, when isolated, did not use natural gas as a carbon source. In mixed culture the Trichoderma is thought to grow on metabolites produced by either or both the Graphium and the acid-tolerant bacterium during oxidation of natural gas. The nature of the relationship is being investigated. The mixed culture has been under continuous cultivation for 18 months.

Carbon nanospheres as an anode material for lithium ion batteries with excellent rate capability

RSC Advances ◽  
2015 ◽  
Vol 5 (68) ◽  
pp. 55348-55352 ◽  
Author(s):  
Chaojun Cui ◽  
Xingchuan Sun ◽  
Xianchang Li ◽  
Chengbo Li ◽  
Yongsheng Niu
Keyword(s):  
Carbon Source ◽  
Natural Gas ◽  
Lithium Ion Batteries ◽  
Anode Material ◽  
Rate Capability ◽  
Lithium Ion ◽  
Carbon Nanospheres

Carbon nanospheres (CNSs) are prepared with natural gas as a carbon source.

scholarly journals Reductive, Coenzyme A-Mediated Pathway for 3-Chlorobenzoate Degradation in the Phototrophic BacteriumRhodopseudomonas palustris

2001 ◽  
Vol 67 (3) ◽  
pp. 1396-1399 ◽  
Author(s):  
Paul G. Egland ◽  
Jane Gibson ◽  
Caroline S. Harwood
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Reductive Dechlorination ◽  
Coenzyme A ◽  
Rhodopseudomonas Palustris ◽  
Aromatic Acid ◽  
Enzyme Assays ◽  
Acetyl Coa ◽  
Selective Enrichment ◽  
Anaerobic Dechlorination

ABSTRACT We isolated a strain of Rhodopseudomonas palustris(RCB100) by selective enrichment in light on 3-chlorobenzoate to investigate the steps that it uses to accomplish anaerobic dechlorination. Analyses of metabolite pools as well as enzyme assays suggest that R. palustris grows on 3-chlorobenzoate by (i) converting it to 3-chlorobenzoyl coenzyme A (3-chlorobenzoyl–CoA), (ii) reductively dehalogenating 3-chlorobenzoyl–CoA to benzoyl-CoA, and (iii) degrading benzoyl-CoA to acetyl-CoA and carbon dioxide.R. palustris uses 3-chlorobenzoate only as a carbon source and thus incorporates the acetyl-CoA that is produced into cell material. The reductive dechlorination route used by R. palustris for 3-chlorobenzoate degradation differs from those previously described in that a CoA thioester, rather than an unmodified aromatic acid, is the substrate for complete dehalogenation.

scholarly journals Diversity of microbial communities in open mixed culture fermentations: impact of the pH and carbon source

2008 ◽  
Vol 80 (6) ◽  
pp. 1121-1130 ◽  
Author(s):  
Margarida F. Temudo ◽  
Gerard Muyzer ◽  
Robbert Kleerebezem ◽  
Mark C. M. van Loosdrecht
Keyword(s):  
Carbon Source ◽  
Microbial Communities ◽  
Mixed Culture

Effect of carbon source on the formation of polyhydroxyalkanoates (PHA) by a phosphate-accumulating mixed culture

1998 ◽  
Vol 22 (8) ◽  
pp. 662-671 ◽  
Author(s):  
P.C Lemos ◽  
C Viana ◽  
E.N Salgueiro ◽  
A.M Ramos ◽  
J.P.S.G Crespo ◽  
...  
Keyword(s):  
Carbon Source ◽  
Mixed Culture

Isolation, characterization, and identification ofGeobacillus thermodenitrificansHRO10, an α-amylase and α-glucosidase producing thermophile

2005 ◽  
Vol 51 (8) ◽  
pp. 685-693 ◽  
Author(s):  
Thaddeus C Ezeji ◽  
Arite Wolf ◽  
Hubert Bahl
Keyword(s):  
Carbon Source ◽  
Type Strain ◽  
Culture Supernatant ◽  
Amylase Activity ◽  
Inorganic Nitrogen ◽  
Hydrolysis Product ◽  
Aerobic Bacteria ◽  
Optimum Temperature ◽  
Geobacillus Thermodenitrificans ◽  
Selective Enrichment

Thermophilic and amylolytic aerobic bacteria were isolated from soil through a selective enrichment procedure at 60 °C with starch as the carbon source. One of the isolates designated as HRO10 produced glucose aside from limit dextrin as the only hydrolysis product from starch and was characterized in detail. The starch-degrading enzymes produced by strain HRO10 were determined to be α-amylase and α-glucosidase. Whereas the α-amylase activity was detected exclusively in the culture supernatant, α-glucosidase occurred intracellular, extracellular, or on the surface of the bacteria depending on the growth phase. The optimum temperature and pH required for the growth of strain HRO10 were about 50 °C and pH 6.5 to 7.5. The strain used different carbohydrates as the carbon source, but the maximum production of α-amylase occurred when 1.0% (w/v) starch or dextrin was used. The use of organic vs. inorganic nitrogen favored the production of α-amylase in strain HRO10. The metal ions Li+, Mg2+, and Mn2+stimulated the production of both enzymes. Identification of strain HRO10 by physiological and molecular methods including sequencing of the 16S rDNA showed that this strain belongs to the species Geobacillus thermodenitrificans. Biochemically, strain HRO10 differs from the type strain DSM 465 only in its ability to hydrolyze starch.Key words: thermophilic, amylolytic, α-amylase, α-glucosidase, Geobacillus thermodenitrificans.

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