Chemical composition of cell walls of Saccharomyces fragilis

1969 ◽  
Vol 15 (9) ◽  
pp. 989-993 ◽  
Author(s):  
T. Reuvers ◽  
E. Tacoronte ◽  
C. Garcia Mendoza ◽  
M. Novaes-Ledieu
Keyword(s):  
Amino Acids ◽  
Chemical Composition ◽  
Cell Walls ◽  
Carbohydrate Content ◽  
Positive Component ◽  
High Carbohydrate ◽  
Mechanical Disruption

Cell walls of Saccharomyces fragilis grown in two different media were prepared by mechanical disruption, and their chemical composition was studied. A high carbohydrate content (75–80%) was found in these walls. Glucose and mannose were represented in a ratio 1:1 in both cases; cell walls also contained a small amount of glucosamine. Sixteen common amino acids were detected in S. fragilis wall hydrolysates. One unidentified ninhydrin-positive component was present when the organism was grown in Hansen medium. A low percentage of lipid was found in both walls. The significance of all these data is discussed in the light of the difficulties encountered in the preparation and purification of walls.

Staphylococcal polysaccharide products inhibitory to Staphylococcus aureus bacteriophage

1975 ◽  
Vol 21 (8) ◽  
pp. 1178-1184
Author(s):  
E. R. Scheer ◽  
B. W. Koft
Keyword(s):  
Amino Acids ◽  
Staphylococcus Aureus ◽  
Inhibitory Activity ◽  
Uronic Acid ◽  
Active Material ◽  
Carbohydrate Content ◽  
Exact Nature ◽  
High Carbohydrate

Four products derived from Staphylococcus aureus cultures were partially purified and tested for inhibitory activity against staphylococcal phage. Phage inhibition, a specific stable phenomenon, was concentration dependent. All inhibitory products contained carbohydrate and amino acids, the most active (phage 73 lysate product) having a high carbohydrate content. Galactose, glucosamine, five or six amino acids, and possibly 3-O-methylglucose and a uronic acid were found as components in all active preparations. However, the exact nature of the active material remains undetermined.

scholarly journals Phytochemical and Psychotropic Research of Motherwort (Leonurus cardiaca L.) Modified Dry Extracts

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 230
Author(s):  
Oleh Koshovyi ◽  
Ain Raal ◽  
Igor Kireyev ◽  
Nadiya Tryshchuk ◽  
Tetiana Ilina ◽  
...  
Keyword(s):  
Amino Acids ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Pharmacological Activity ◽  
Ellagic Acid ◽  
Hydroxycinnamic Acids ◽  
Psychotropic Activity ◽  
Phytochemical Analysis ◽  
Phenolic Substances ◽  
Leonurus Cardiaca

The prospect of creating a new medicine with psychotropic activity is shown as a result of studying the chemical composition and pharmacological activity of modified dry extracts of motherwort (Leonurus cardiaca L.) tincture. The most promising substances were the dry extracts, modified by adding small amounts of arginine, valine, phenylalanine, glycine, lysine, and alanine. A total of 15 main phenolic substances were found in the extracts, and eight of them were identified. There were also 10 hydroxycinnamic acids in these extracts, three of which were identified (chlorogenic, caffeic, and rosmarinic acids). The dominant hydroxycinnamic acids were chlorogenic and caffeic acids. Among flavonoids, catechin, hyperoside, and rutin were identified. It should be noted that the extracts had a significant content of ellagic acid. On the basis of the results of the phytochemical analysis of the extracts, it can be concluded that the composition of phenolic compounds does not differ significantly, and the main differences are related to amino acids, which obviously have an impact on the overall pharmacological effect. The results obtained indicate the presence of anxiolytic activity in the motherwort extracts studied in complex with amino acids. The extracts with glycine, valine, and arginine were more effective in reducing anxiety in animals.

Differential Effects of High-fat and High-carbohydrate Content Isoenergetic Meals on Plasma Active Ghrelin Concentrations in Lean and Obese Women

2004 ◽  
Vol 36 (8) ◽  
pp. 559-563 ◽  
Author(s):  
N. Tentolouris ◽  
A. Kokkinos ◽  
C. Tsigos ◽  
D. Kyriaki ◽  
J. Doupis ◽  
...  
Keyword(s):  
Carbohydrate Content ◽  
Obese Women ◽  
High Fat ◽  
Differential Effects ◽  
High Carbohydrate

scholarly journals Chemical composition and nutritional quality of short-ripened rennet cheeses produced by traditional methods

2018 ◽  
Vol 74 (10) ◽  
pp. 5971-2018 ◽  
Author(s):  
PRZEMYSŁAW KNYSZ ◽  
MICHAŁ GONDEK ◽  
RENATA PYZ-ŁUKASIK ◽  
MONIKA ZIOMEK ◽  
ŁUKASZ DROZD ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Chemical Composition ◽  
G Protein ◽  
Nutritive Value ◽  
Salt Content ◽  
Amino Acid Content ◽  
Traditional Methods ◽  
Dairy Starter Cultures ◽  
Limiting Amino Acids

The aim of the study was to determine the variability in the chemical composition and nutritive value parameters of smoked and unsmoked short-ripened rennet cheeses and unsmoked long-ripened rennet cheeses produced by traditional methods. The raw material for the production of short-ripened cheeses was pasteurized cows’ milk obtained from a dairy, whereas the long-ripened cheeses were manufactured from raw cows’ milk obtained from the producer’s farm. All three varieties of cheese examined were produced with commercial dairy starter cultures. The material for the study was collected in winter, directly at the producers’ retail outlets in southern and eastern Poland. The basic chemical composition was determined according to the Polish Standards, whereas the amino acid profiles of proteins from the cheeses were determined by ion-exchange chromatography. The result analysis revealed significant differences between the different varieties of cheese in terms of their water content, ranging from 32.2% to 42.1%, as well as protein content, which varied from 25.6% to 31.6%. Fat levels ranged between 22.2% and 24%, whereas total ash content amounted to 5.1-5.8%. The significantly highest salt content was found in unsmoked short-ripened cheeses. In all three cheese varieties, the total exogenous amino acid content was comparable, ranging from 46.17 g to 47.36 g/100 g protein, and that of endogenous amino acids varied from 52 g to 53 g/100 g protein. The biological value of proteins was determined by calculating to the chemical score (CS), as described by Mitchell and Block, and the essential amino acid index (EAAI), as described by Oser. A comparison of the results with the standard chicken egg white proteins showed that the limiting amino acids for all varieties of cheese were methionine and cysteine. On the other hand, a comparison with the FAO/WHO-suggested pattern of amino acid requirements (1991) for all age groups over 1 year of age showed that the limiting amino acids were methionine and cysteine in smoked and unsmoked short-ripened cheeses, and treonine in long-ripened cheeses. Considering the chemical indices, such as CS and EAAI, it may be concluded that the traditional rennet cheeses produced in southern and eastern Poland have a favourable amino acid composition of proteins and a high nutritive value..

scholarly journals A Label-free, Fast and High-specificity Technique for Plant Cell Wall Imaging and Composition Analysis

2020 ◽  
Author(s):  
Huimin Xu ◽  
Yuanyuan Zhao ◽  
Yuanzhen Suo ◽  
Yayu Guo ◽  
Yi Man ◽  
...  
Keyword(s):  
Cell Wall ◽  
Chemical Composition ◽  
Raman Scattering ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Composition Analysis ◽  
Plant Cell Walls ◽  
Label Free ◽  
Wall Imaging

Abstract Background: Cell wall imaging can considerably permit direct visualization of the molecular architecture of cell walls and provide the detailed chemical information on wall polymers, which is imperative to better exploit and use the biomass polymers; however, detailed imaging and quantifying of the native composition and architecture in the cell wall remains challenging.Results: Here, we describe a label-free imaging technology, coherent Raman scattering microscopy (CRS), including coherent anti-Stokes Raman scattering (CARS) microscopy and stimulated Raman scattering (SRS) microscopy, which images the major structures and chemical composition of plant cell walls. The major steps of the procedure are demonstrated, including sample preparation, setting the mapping parameters, analysis of spectral data, and image generation. Applying this rapid approach, which will help researchers understand the highly heterogeneous structures and organization of plant cell walls.Conclusions: This method can potentially be incorporated into label-free microanalyses of plant cell wall chemical composition based on the in situ vibrations of molecules.

scholarly journals Morphoanatomical and histochemical studies of the seed development of Euterpe oleracea (Arecaceae)

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
João Alves Ferreira Pereira ◽  
Ítalo Antônio Cotta Coutinho ◽  
Emanoella Lima Soares ◽  
Arlete Aparecida Soares ◽  
Ana Paula de Souza Caetano ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Seed Development ◽  
Cell Walls ◽  
Palm Tree ◽  
Euterpe Oleracea ◽  
Scientific Interest ◽  
Vast Array ◽  
Future Studies ◽  
Seed Biology ◽  
Fruit Pericarp

Abstract Although the consumption of açaí (Euterpe oleracea) pulp has long been an important component of the diet of the peoples from the Amazon, the açaí palm tree has recently attracted economic and scientific interest because of its vast array of bioactive compounds found in the fruit pericarp. The açaí seeds are the largest byproduct after pulp extraction and have potential for use in ethanol production, but this process is hindered by limited knowledge of seed biology, chemical composition and pattern reserve deposition during seed development. The aim of this work was to describe the morphoanatomical development of the seeds, as well as to identify the main organic compounds stored in the seeds. To achieve this goal, histological and histochemical analyses were performed on developing seeds. Results showed the seed is albuminous, bitegmic and that ingrowths of the seed coat give rise to a ruminate endosperm. Moreover, the nutritive reserves of açaí seeds are found in the endosperm thickened cell walls as reserve polysaccharides. Our findings provide information for future studies dealing with reproductive biology, propagation and the improvement of this profitable crop.

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