Production of tetrathionate reductase by Salmonella

1969 ◽  
Vol 15 (2) ◽  
pp. 238-240 ◽  
Author(s):  
J. Papavassiliou ◽  
Vasso Samaraki-Lyberopoulou ◽  
G. Piperakis
Keyword(s):  
Escherichia Coli ◽  
Paratyphi B ◽  
Salmonella Serotypes

From 195 strains of Salmonella, 175 (89.8%) produced tetrathionate reductase (TTR). TTR-negative Salmonella were found in 7 out of 31 serotypes examined: S. paratyphi A, S. paratyphi B, S. typhimurium, S. paratyphi C, S. thompson, S. typhi, and S. enteritidis. TTR-negative S. paratyphi B and S. thompson have not previously been reported. Production of TTR is valuable for the subdivision of some Salmonella serotypes into TTR-positive and TTR-negative variants.Examination of 362 strains of Enterobacteriaceae for TTR confirmed that Escherichia coli, Shigella, Klebsiella, and Aerobacter cloacae (enterobacter) are TTR-negative, while Salmonella, Citrobacter, and Proteus are usually TTR-positive.

scholarly journals Salmonellas in sewage. A study in latent human infection

1969 ◽  
Vol 67 (3) ◽  
pp. 517-523 ◽  
Author(s):  
R. W. S. Harvey ◽  
T. H. Price ◽  
D. W. Foster ◽  
W. C. Griffiths
Keyword(s):  
Technical Assistance ◽  
Human Infection ◽  
Reference Laboratory ◽  
Salmonella Infection ◽  
Technical Success ◽  
Sewerage System ◽  
The Public ◽  
Multiple Sampling ◽  
Paratyphi B ◽  
Salmonella Serotypes

SUMMARYIn a residential estate of 4000 persons, containing neither industry nor retail butchers shops, salmonellas were regularly found in the sewerage system. They were frequently found in the sewage of a portion of the estate housing 1000 persons. The range of serotypes found was wide and some types suggested an exotic origin. No overt salmonella infection in the estate was reported during the period of survey, although local general practitioners had been previously alerted. Overt infection due to serotypes found in the survey were, however, reported in other areas of Glamorgan. Multiple sampling points in the sewerage system and a serological technique for examining samples contaminated with multiple salmonella serotypes were essential for the technical success of the survey.We should like to thank Prof. Scott Thomson for his advice in the preparation of this paper; Dr E. S. Anderson of the Central Enteric Reference Laboratory and Bureau, Colindale, for phage-typing the strains of S. typhimurium and S. paratyphi B; and Dr G. J. G. King of the Public Health Laboratory, Bournemouth, for identifying the serotypes isolated. We should also like to thank Mr T. R. Liddington and Mr J. H. Price for their technical assistance.

Mutations in gyrA gene of quinolone-resistant Salmonella serotypes isolated from humans and animals.

1996 ◽  
Vol 40 (4) ◽  
pp. 1009-1013 ◽  
Author(s):  
D J Griggs ◽  
K Gensberg ◽  
L J Piddock
Keyword(s):  
Escherichia Coli ◽  
Novel Mutation ◽  
Quinolone Resistance ◽  
Gyra Gene ◽  
Salmonella Serotypes

The quinolone resistance-determining regions (QRDRs) of the gyrA genes of quinolone-resistant clinical and veterinary salmonella isolates were sequenced. Substitutions analogous to a substitution of a Ser to a Phe at position 83 (Ser83-->Phe) and Asp87-->Gly or Tyr in Escherichia coli were found, as was a single novel mutation outside of the QRDR resulting in Ala119-->Glu. The data suggest that gyrA mutations are associated with quinolone resistance in veterinary and clinical salmonella isolates and that the limits of the QRDR may require revision.

Cloning and characterization of the bundle-forming pilin gene of enteropathogenic Escherichia coli and its distribution in Salmonella serotypes

1993 ◽  
Vol 7 (4) ◽  
pp. 563-575 ◽  
Author(s):  
Indira Sohel ◽  
Jose Luis Puente ◽  
William J. Murray ◽  
Jaana Vuopio-Varkila ◽  
Gary K. Schoolnik
Keyword(s):  
Escherichia Coli ◽  
Enteropathogenic Escherichia Coli ◽  
Salmonella Serotypes ◽  
Pilin Gene

Salmonella Serotypes Isolated In Malaysia Over The Ten-year Period 1983-1992

1997 ◽  
Vol 9 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Rohani Md Yasin ◽  
M Jegathesan ◽  
Cheah Cheng Tiew
Keyword(s):  
Paratyphi B ◽  
Salmonella Serotypes

From January 1983 to December 1992 a total of 20,874 salmonella were serotyped in the Bacteriology Division IMR, which showed an increase of 100% compared to the previous ten-years. There were 97 serotypes which belonged to 22 Kauffmann-white groups. Twenty two serotypes hitherto were seen in this study period. S. typhi was the commonest serotype isolated. Overall there was a rise in the isolation of non-typboidal salmonella particularly S. enteritidis which increased by 760% and S. blockley which increased by 720%. However there is a drop in the isolation of S typhimurium by 223% and S. paratyphi B by 319%.

Characterization of Escherichia coli and Salmonella from Victoria, Australia, Dairy Farm Environments

2017 ◽  
Vol 80 (12) ◽  
pp. 2078-2082 ◽  
Author(s):  
Catherine M. McAuley ◽  
Kate E. McMillan ◽  
Sean C. Moore ◽  
Narelle Fegan ◽  
Edward M. Fox
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Dairy Farm ◽  
Farm Animals ◽  
E Coli ◽  
Virulence Markers ◽  
Salmonella Serotypes ◽  
Serotype Identification ◽  
Salmonella Serotype ◽  
Genetic Profiles

ABSTRACT Safe dairy food production starts at the farm level, with the presence of pathogens on farms potentially impacting the downstream food supply. Studies often commence with looking for pathogens in fecal material of farm animals, predominantly cows; however, pathogens may arise from other on-farm sources. In Australia, few studies have looked at the broader farm environment, particularly in relation to Escherichia coli and Salmonella. The present study characterized the genetic similarity of these pathogens from bovine, ovine, and caprine dairy farm environments and related this to the stx1, stx2, eae, or ehx virulence markers in E. coli and antibiotic resistance in Salmonella. E. coli isolates with indistinguishable genetic profiles and at least one of the virulence factors were found in multiple samples on the farms, although profiles were unique to each farm. E. coli O26 with stx1 from one bovine farm had a different fingerprint type than all of the other E. coli O26 isolates, which lacked the Shiga toxin genes. They were from a separate bovine farm and were themselves closely related. No antibiotic resistance was detected among Salmonella isolates to the 17 antibiotics tested. Three Salmonella serotypes were identified: Orion, Infantis, and Zanzibar. The published PCR serotyping method used misidentified Salmonella Zanzibar as Salmonella Javiana, which was revealed after conventional antisera-based serotyping; this illustrates the need for caution when using PCR techniques for Salmonella serotype identification. Of the three serotypes, Salmonella Orion was most prevalent and was potentially resident on the farm. This article describes the previously unreported genetic diversity of potentially pathogenic E. coli and Salmonella serotypes from the farm environments of three dairy animal species in Victoria, Australia.

Incidence and Behavior of Salmonella and Escherichia coli on Whole and Sliced Zucchini Squash (Cucurbita pepo) Fruit

2010 ◽  
Vol 73 (8) ◽  
pp. 1423-1429 ◽  
Author(s):  
JAVIER CASTRO-ROSAS ◽  
EVA MARÍA SANTOS LÓPEZ ◽  
CARLOS ALBERTO GÓMEZ-ALDAPA ◽  
CESAR ABELARDO GONZÁLEZ RAMÍREZ ◽  
JOSÉ ROBERTO VILLAGOMEZ-IBARRA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Inoculum Level ◽  
Probable Number ◽  
Initial Inoculum ◽  
E Coli ◽  
Thermotolerant Coliforms ◽  
Salmonella Serotypes ◽  
And Behavior

The incidence of coliform bacteria (CB), thermotolerant coliforms (TC), Escherichia coli, and Salmonella was determined for zucchini squash fruit. In addition, the behavior of four serotypes of Salmonella and a cocktail of three E. coli strains on whole and sliced zucchini squash at 25 ± 2°C and 3 to 5°C was tested. Squash fruit was collected in the markets of Pachuca city, Hidalgo State, Mexico. CB, TC, E. coli, and Salmonella were detected in 100, 70, 62, and 10% of the produce, respectively. The concentration ranged from 3.8 to 7.4 log CFU per sample for CB, and <3 to 1,100 most probable number per sample for TC and E. coli. On whole fruit stored at 25 ± 2°C or 3 to 5°C, no growth was observed for any of the tested microorganisms or cocktails thereof. After 15 days at 25 ± 2°C, the tested Salmonella serotypes had decreased from an initial inoculum level of 7 log CFU to <1 log, and at 3 to 5°C they decreased to approximately 2 log. Survival of E. coli was significantly greater than for the Salmonella strains at the same times and temperatures; after 15 days, at 25 ± 2°C E. coli cocktail strains had decreased to 3.4 log CFU per fruit and at 3 to 5°C they decreased to 3.6 log CFU per fruit. Both the Salmonella serotypes and E. coli strains grew when inoculated onto sliced squash: after 24 h at 25 ± 2°C, both bacteria had grown to approximately 6.5 log CFU per slice. At 3 to 5°C, the bacterial growth was inhibited. The squash may be an important factor contributing to the endemicity of Salmonella in Mexico.

scholarly journals Molecular Cloning and Characterization of the Salmonella enterica Serovar Paratyphi B rma Gene, Which Confers Multiple Drug Resistance in Escherichia coli

2002 ◽  
Vol 46 (2) ◽  
pp. 360-366 ◽  
Author(s):  
Mahmoud. A. Yassien ◽  
Hosam E. Ewis ◽  
Chung-Dar Lu ◽  
Ahmed T. Abdelal
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Salmonella Enterica ◽  
Genomic Library ◽  
Multiple Drug Resistance ◽  
Protein Profile ◽  
Multiple Drug ◽  
E Coli ◽  
Paratyphi B ◽  
High Degree

ABSTRACT A genomic library from a strain of Salmonella enterica serovar Paratyphi B that exhibits multiple drug resistance (MDR) was constructed in Escherichia coli. Two of the recombinant plasmids, pNOR5 and pNOR5, conferred resistance only to fluoroquinolones in E. coli, whereas the third, pNCTR4, conferred the MDR phenotype. Sequence and subcloning analysis showed that it is the presence of RecA on the first two plasmids which confers resistance to fluoroquinolones in E. coli. A similar analysis established that the MDR phenotype conferred by pNCTR4 is due to a gene, rma (resistance to multiple antibiotics), which encodes a 13.5-kDa polypeptide. The derived sequence for Rma exhibits a high degree of similarity to those of a group of MarA-like activators that confer MDR in E. coli. A MalE-Rma fusion protein was purified to near homogeneity and was shown to interact with a DNA fragment carrying a MarA operator sequence. Furthermore, overexpression of rma in E. coli caused changes in the outer membrane protein profile that were similar to those reported for MarA. These results suggest that Rma might act as a transcriptional activator of the marA regulon.

scholarly journals ANTIBACTERIAL ACTIVITY LEAF EXTRACTS OF STRYCHNOS NUX VOMICA L. - A MEMBER OF LOGANIACEAE

2016 ◽  
Vol 3 (2) ◽  
pp. 59-61
Author(s):  
Nantha Kumar R ◽  
Abdul Kaffoor H ◽  
Arumugasamy K
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Aspergillus Flavus ◽  
Positive Control ◽  
Leaf Extracts ◽  
Penicillium Notatum ◽  
Paratyphi B ◽  
Alternaria Alternate

The present study investigates the antibacterial activity of various solvents leaf extracts of Strychnos nux vomica against four different bactria strains like Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella paratyphi b, Alternaria alternate, Aspergillus flavus, Penicillium notatum and Cladosporium carrionii. All the results were compared with respective positive control.

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