Fungistatic properties of soils exposed to different antecedent environments
A Toledo silty clay soil with different cropping histories was tested for fungistatic activity by an agar-ring method using Bipolaris sorokiniana (Sacc.) Shoemaker as the assay organism. Although all soils exhibited considerable fungistatic activity, the magnitude varied only slightly with their cropping histories. Fungistatic activity of soil cropped to alfalfa was somewhat higher than that of soil obtained from rotation plots with oats or soybeans as the last crop. Attempts were made to extract fungistatic material from soil using water, ethanol, or ether. Although the fungistatic properties of the extracts were lost when sterilized by steam or filtration, nonsterilized extracts were inhibitory to the test fungus.Nutrient studies revealed that fungistasis was suppressed when sucrose, peptone, or yeast extract was added to the soil. Evidence was obtained that soil toxin penetrated Millipore filters and cellophane when they were used as substrates in bioassay of soil fungistasis. Fritted glass, clay filters, Saran wrap, and parchment paper were unsuitable for detecting fungistasis since they were inhibitory to the assay fungus. Evidence in support of the theory that food material added to or along with the test conidia increased soil fungistasis was not demonstrated. Conversely, our findings suggest that soil fungistasis is a natural phenomenon, which is characteristic of certain soils.