Infection bactérienne à Salmonella typhimurium chez un invertébré, Galleria mellonella L.

1968 ◽  
Vol 14 (3) ◽  
pp. 233-237 ◽  
Author(s):  
E. Kurstak ◽  
C. E. Vega
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Galleria Mellonella ◽  
Salmonella Infection ◽  
Total Hemocyte Count ◽  
Hemocyte Count ◽  
Infection Bactérienne ◽  
Epidemiological Significance ◽  
Number Of Bacteria ◽  
Wax Moth

Fourth and fifth instar larvae of Galleria mellonella were inoculated by injection or "per os" with two strains of Salmonella typhimurium. After injection of 2 × 104 viable units, the average mortality was 68%, with strain 7 Suc LL, and 26% with strain LT2; by per os inoculation, mortality was 58% and 18% respectively. During infection, the number of bacteria increased and the total hemocyte count decreased by 64% with a consequent impairment of phagocytosis. Infection under similar conditions with a saprophytic Enterobacteriaceae species, Escherichia coli had no effect. Since the wax-moth infests beehives the possibility of Salmonella infection may be of epidemiological significance.

scholarly journals Activation of cellular immune response in insect model host Galleria mellonella by fungal α-1,3-glucan

2020 ◽  
Vol 78 (9) ◽  
Author(s):  
Sylwia Stączek ◽  
Agnieszka Zdybicka-Barabas ◽  
Adrian Wiater ◽  
Małgorzata Pleszczyńska ◽  
Małgorzata Cytryńska
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Plant Pathogens ◽  
Galleria Mellonella ◽  
Pathogenic Fungi ◽  
Fungal Cell ◽  
Metarhizium Acridum ◽  
Total Hemocyte Count ◽  
Hemocyte Count ◽  
Cellular Immune

Abstract Alpha-1,3-glucan, in addition to β-1,3-glucan, is an important polysaccharide component of fungal cell walls. It is reported for many fungal species, including human pathogenic genera: Aspergillus, Blastomyces, Coccidioides, Cryptococcus, Histoplasma and Pneumocystis, plant pathogens, e.g. Magnaporthe oryzae and entomopathogens, e.g. Metarhizium acridum. In human and plant pathogenic fungi, α-1,3-glucan is considered as a shield for the β-1,3-glucan layer preventing recognition of the pathogen by the host. However, its role in induction of immune response is not clear. In the present study, the cellular immune response of the greater wax moth Galleria mellonella to Aspergillus niger α-1,3-glucan was investigated for the first time. The changes detected in the total hemocyte count (THC) and differential hemocyte count (DHC), formation of hemocyte aggregates and changes in apolipophorin III localization indicated activation of G. mellonella cellular mechanisms in response to immunization with A. niger α-1,3-glucan. Our results, which have clearly demonstrated the response of the insect immune system to this fungal cell wall component, will help in understanding the α-1,3-glucan role in immune response against fungal pathogens not only in insects but also in mammals, including humans.

scholarly journals Establishing an invertebrate Galleria mellonella greater wax moth larval model of Neisseria gonorrhoeae infection

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 1900-1920
Author(s):  
Aiste Dijokaite ◽  
Maria Victoria Humbert ◽  
Emma Borkowski ◽  
Roberto M La Ragione ◽  
Myron Christodoulides
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Galleria Mellonella ◽  
Greater Wax Moth ◽  
Wax Moth
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