Extracellular proteolytic enzymes of psychrophilic bacteria. II. Production and heterogeneity of enzymes of an obligately psychrophilic red-pigmented bacterium and of a white variant

1968 ◽  
Vol 14 (3) ◽  
pp. 225-231 ◽  
Author(s):  
Y. Nunokawa ◽  
I. J. McDonald

The appearance of proteolytic enzymes in culture fluids during growth of an obligately psychrophilic red-pigmented bacterium and of a white variant was followed. Enzymes were precipitated with ammonium sulfate and fractionated on Sephadex G-100. Both organisms produced a slow-moving component (III) at the start of proteinase production and a fast-moving component as the culture aged. The fast-moving component of the red but not of the white organism was separated into two fractions (I-1 and I-2) on DEAE-cellulose. On filtration through Sephadex, fraction I-1 gave rise to fraction III; fraction I-2 gave rise to enzymatically active I-2 and inactive red pigment. It is suggested that both organisms produce fraction III initially and that this aggregates to form fraction I-1 and that the red organism produces fraction I-2, which is a separate protein.

1968 ◽  
Vol 14 (3) ◽  
pp. 215-224 ◽  
Author(s):  
Y. Nunokawa ◽  
I. J. McDonald

Proteinase in culture fluids of an obligately psychrophilic bacterium was precipitated by ammonium sulfate and fractionated by gel filtration and DEAE-cellulose chromatography. Three purified fractions (I-1, I-2, and III-1) with proteinase activity were obtained. On the basis of reactions and characteristics (i.e. effect of pH, heat, and metal ions on activity and stability, hydrolysis of synthetic peptides and of natural proteins) fractions I-1 and III-1 appeared to be very similar whereas fraction I-2 was different. When proteinase preparations were examined by electrophoresis, fractions I-1 and III-1 gave similar patterns; fraction I-2 gave a different one. From the results it is suggested that the organism produces two proteinases and that possibly fraction I-1 represents an aggregation of molecules of III-1 and that fraction I-2 is a different proteinase.


1962 ◽  
Vol 07 (02) ◽  
pp. 230-238 ◽  
Author(s):  
A Pavlovsky ◽  
H Peterson ◽  
G Casillas ◽  
C Simonetti ◽  
A Martinez Canaveri ◽  
...  

SummaryThis publication describes the results obtained by treatment of haemophiliacs with factor VIII preparations isolated from Cohn fraction I by use of tannic acid, FI-O-Ta.The authors stress the rapidity of the disappearance of factor VIII after injection. Transfusions are generally well tolerated. One reaction of the pyrogen type has been observed and also a case of activation of the fibrinolytic system.A second purification method by means of chromatography on DEAE-cellulose is described.


Humaniora ◽  
2011 ◽  
Vol 2 (2) ◽  
pp. 1309
Author(s):  
Handy Martinus

Fast moving in retail industry is the latest trading industry in trading chain. The study is to identify the condition of retail industry in Indonesia. This uses normative juridical approach. The research material is gathered by literature or document review. The analysis for modern retail industry is conducted through qualitative, thus explaining law material gathered from library being selected, arranging systematically, and finally getting conclusion figured to answer problems related to the rules for monopoly and dysfunction business competition in retail industry. The study result showed that modern market, which so far having a good performance, will have challenges. One biggest challenge is the potential for slow-moving revenue growth as the effects of slow-moving economic caused by global crisis.  


2021 ◽  
Vol 15 (1) ◽  
pp. 60
Author(s):  
Amri Yanuar ◽  
Marwanto Rahmatuloh ◽  
Ilman Mutaqien

Operasional gudang memiliki berbagai aktivitas inti yang saling berkaitan satu dengan yang lainnya, seperti aktivitas receiving, storage, dan picking. Aktivitas inti ini atau yang biasa disebut inbound dan outbound harus dikerjakan dalam waktu yang singkat dan meminimalisir tingkat kesalahan agar tidak mengganggu keberlangsungan alur masuk dan keluarnya barang di gudang. Beberapa hal yang dapat mengganggu aliran barang di gudang di antaranya adalah terjadinya bottle neck di area receiving, waktu proses pencarian barang yang lama, dan 70% waktu proses order picking di bawah waktu standar. Permasalahan-permasalahan ini terjadi salah satunya dikarenakan pengelolaan barang di gudang masih manual atau belum menggunakan sistem informasi. Tujuan dari penelitian ini adalah merancang sistem informasi untuk membantu operasional gudang guna meminimalisir kesalahan-kesalahan yang dapat mengganggu aliran masuka dan keluarnya barang dari gudang, dengan menggunakan pendekatan FSN Analysis. Hasil dari pengolahan data menggunakan FSN Analysis ini, dapat membantu dalam mengetahui produk-produk yang masuk kedalam kategori fast moving, slow moving, dan non-moving.


1978 ◽  
Vol 3 (4) ◽  
pp. 249-250 ◽  
Author(s):  
K. V. Sheridan ◽  
B. V. Jackson ◽  
D. J. McLean ◽  
G. A. Dulk

Studies of coronal transients observed in white-light (Gosling et al., 1976) have shown that fast-moving events (≤ 400 km s-1) are closely associated with flares and with type II and IV radio bursts while slow-moving events are not. We now report the first detection of the radio counterpart of a slow-moving transient. The event of 1974 January 21 is shown to be visible on maps of the quiet Sun made at a frequency of 80 MHz.


1994 ◽  
Vol 20 ◽  
pp. 183-186 ◽  
Author(s):  
S. Anandakrishnan ◽  
R. B. Alley

Microearthquakes at the base of slow-moving Ice Stream C occur many times more frequently than at the base of fast-moving Ice Stream B. We suggest that the microearthquake source sites are so-called “sticky spots”, defined as limited zones of stronger Subglacial material interspersed within a weaker matrix. The fault-plane area of the microearthquakes (O(102m2)) is therefore a measure of the size of the sticky spots. The spatial density of the microearthquakes (O(10 km-2)) is a measure of the distribution of sticky spots.The average stress drop associated with these microearthquakes is consistent with an ice-stream bed model of weak subglacial till interspersed with stronger zones that support much or all of the basal shear stress. We infer a weak inter-sticky-spot material by the large distances (O(103m)), relative to fault radius, to which the microearthquake stress change is transmitted.


Author(s):  
Clyde A. Leys

ABSTRACTThe Recent Saefell tuff-ring on Heimaey, Iceland was formed by surtseyan activity in shallow seawater. The tuff-ring has a basal diameter of about 3 km, a maximum rim height of 188 m above sea-level and a crater diameter of 1300 m. Three tuff-units are recognised, separated by unconformities on and inside the crater rim due to syndepositional slumping. The crater contains a nested rim which was constructed above slumped crater tuffs. Directional data indicate strongly directed blasts to the SW at a late stage in the activity.Throughout the volcanic activity, base-surges formed antidunes, U-shaped channels, vesiculated tuffs, small ripples and plastering structures. One antidune reflects a decrease in surge flow power during deposition and subsequent slumping due to base-surge drag and instabilities developed during growth. On the basis of field characteristics, the structures are divided into those deposited by hot, dry, fast-moving surges and those by cooler, wet, slow-moving surges. Base-surges are compared with turbidity currents and deposition of distinct structures by the head, body and tail regions is interpreted.


1987 ◽  
Vol 65 (11) ◽  
pp. 2682-2689 ◽  
Author(s):  
E. Nol ◽  
D. E. Gaskin

Distribution and movement patterns are described for summering (nonbreeding) Black Guillemots in the Deer Island region of the southwestern Bay of Fundy, Canada. Guillemots were distributed in areas with moderate current velocities (range, 30–68 cm/s) and shallow to intermediate depths (range, 17–31 m). The birds appeared to avoid shallow and deep areas with fast- or slow-moving water. Guillemots preferred islands with extensive underwater ledges, presumably because these harboured sufficient prey and provided protection from fast-moving tidal waters. Guillemots moved in and out of the approaches passively with the tide and, in contrast to larids in the region, made only small-scale flights to reposition themselves in relation to food resources.


2014 ◽  
Vol 97 ◽  
pp. 113-123 ◽  
Author(s):  
Emmanuelle Pouydebat ◽  
Antony Borel ◽  
Hélène Chotard ◽  
Dorothy Fragaszy

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