The mechanism of action of griseofulvin

1968 ◽  
Vol 14 (2) ◽  
pp. 111-118 ◽  
Author(s):  
Floyd M. Huber ◽  
David Gottlieb
Keyword(s):  
Cell Wall ◽  
Botrytis Cinerea ◽  
Aspartic Acid ◽  
Mechanism Of Action ◽  
Dna Content ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Lipid Fraction ◽  
Acid Incorporation ◽  
Total Dna

Griseofulvin had no effect on the respiration of Botrytis cinerea but, nevertheless, inhibited growth and caused abnormal hyphal formations, including stunting, spiraling, thickening of the cell wall, and disorientation of growth. Treated cells had an increase in total deoxyribonucleic acid and phosphorus but not in protein, carbohydrate, lipid, and ribonucleic acid. Griseofulvin-treated cells synthesized DNA from labelled glucose and glycine continuously and for much longer periods than control cells so that their total DNA content was greater than that in untreated cells. However, the antibiotic allowed slightly less incorporation with aspartic acid -U-14C as the precursor than the controls. Griseofulvin caused 25 to 50% increased incorporation of carbon into RNA from glucose and glycine but the increases were not due to a prolongation of the synthetic period, and again aspartic acid incorporation was slightly decreased. Griseofulvin was bound to the particulate parts of the cell and was especially high in the lipid fraction of the cell. The antibiotic was not bound to DNA or to RNA.

Ribosomal ribonucleic acid cistron homologies among Hyphomicrobium and various other bacteria

1977 ◽  
Vol 23 (4) ◽  
pp. 478-481 ◽  
Author(s):  
Richard L. Moore
Keyword(s):  
Cell Wall ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Gram Positive ◽  
Gram Negative ◽  
Group Iii ◽  
Hybrid Formation ◽  
Group I ◽  
Ribosomal Ribonucleic Acid ◽  
Taxonomic Implications

The extent of hybrid formation between the ribosomal ribonucleic acid (r-RNA) of Hyphomicrobium strain B-522 and deoxyribonucleic acid (DNA) from bacteria of 21 different genera was examined. Three generalized groupings were formed. Group I (72–100%) consisted entirely of other strains of Hyphomicrobium. Representatives of the genera Rhodopseudomonas, Chromatium, Caulobacter, Prosthecomicrobium, Rhodomicrobium, Hyphomonas, and Hyphomicrobium made up group II (49–69%). The remaining Gram-negative, Gram-positive, and cell wall – less bacteria fell into group III (12–40%). The taxonomic implications of these results are discussed.

The physiology of growth in Subterranean clover. II. The dynamics of leaf growth

1970 ◽  
Vol 18 (2) ◽  
pp. 149 ◽  
Author(s):  
RF Williams ◽  
. Rijven.A.H.G.C
Keyword(s):  
Cell Wall ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Vascular System ◽  
Leaf Growth ◽  
Subterranean Clover ◽  
Dry Weight ◽  
Protein Nitrogen ◽  
Wall Materials ◽  
Cell Wall Materials

This study extends the account of the development of the fourth leaf of subterranean clover. In particular, the four leaf constituents deoxyribonucleic acid, ribonucleic acid, proteins, and cell wall materials are expressed on whole leaf and cell bases. Form change in the leaf is illustrated with perspective elevations for some early stages. Cell number increases exponentially until day 13, with a mean generation time of 18 hr. The final number per leaf is c. 6.5 million, and deoxyribonucleic acid phosphorus is estimated as 0.197 pg per cell. The leaf is highly succulent, with a maximum water content of 478 % dry weight. Soluble constituents are also high, with a maximum of 39 % of the dry matter. The absolute amounts of ribonucleic acid phosphorus and protein nitrogen are maximal on days 19 and 25 respectively, with losses of about 60% by day 36. Cell wall materials increase from 8 to 57 % of the residual dry weight during a period of 4 days prior to emergence. This is shown to be due to the concurrent differentiation of the vascular system and the growth of 3000 leaf hairs. Changes on a per cell basis are recorded, and the concept of relative growth rate is used to extend the analysis of the data. Rates of production of one constituent per unit of another are also presented and discussed.

scholarly journals Deoxyribonucleic acid-dependent ribonucleic acid polymerases from normal and polyoma-transformed BHK-21/C13 cells

1975 ◽  
Vol 145 (3) ◽  
pp. 509-516 ◽  
Author(s):  
R J Cooper ◽  
H M Keir
Keyword(s):  
Ionic Strength ◽  
Rna Polymerase ◽  
Dna Content ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Actinomycin D ◽  
Activity Ratio ◽  
Cell Types ◽  
Dna Templates ◽  
Alpha Amanitin

DNA-dependent RNA polymerase (EC 2.7.7.6) ACTIVITIES FROM NORMAL BHK-21/C13 cells and from BHK-21/C13 cells transformed by polyoma virus (PYY cells) were solubilized and fractionated on columns of DEAE-Sephadex. Various properties of the A and B enzymes from the two types of cell were compared. 1. The yields of polymerase relative to the DNA content of the nuclear preparations are similar for both cell types. 2. The ionic-strength optima of polymerases A and B are 12.5 mM and 100mM with respect to (NH4)2SO4 for both cell types. 3. The Mn2+/Mg2+ activity ratio (measured at the respective optimum for each cation) for polymerase A from BHK-21/C13 cells was 1.48 and for the polymerase A from PYY cells was 0.55. The corresponding ratios for polymerase B were 10.11 for BHK-21/C13 cells and 22.75 for PYY cells. 4. Minor differences in the ability of the A polymerases to transcribe native and denatured DNA templates were observed; such differences were not apparent when the B polymerases were compared. 5. All the polymerases were inhibited completely by actinomycin D and by rifampicin AF/013, but not markedly so by rifampicin. Alpha-amanitin inhibited polymerase B but not polymerase A.

scholarly journals Mechanism of Action of Ribonucleic Acid-directed Deoxyribonucleic Acid Polymerase

1974 ◽  
Vol 249 (22) ◽  
pp. 7049-7056
Author(s):  
Amy K. Falvey ◽  
Judy A. Kantor ◽  
M.G. Robert-Guroff ◽  
Dante J. Picciano ◽  
Gary B. Weiss ◽  
...  
Keyword(s):  
Mechanism Of Action ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid

The DNA content of single cells of Micrococcus radiodurans

1970 ◽  
Vol 16 (11) ◽  
pp. 1136-1137 ◽  
Author(s):  
A. A. Driedger
Keyword(s):  
Cell Wall ◽  
Dna Content ◽  
Deoxyribonucleic Acid ◽  
Single Cells ◽  
Phase Cell ◽  
Logarithmic Phase ◽  
Cell Counts ◽  
Controlled Treatment

Single cells of Micrococcus radiodurans may be liberated from their cell wall by a controlled treatment with lysozyme, thus making accurate cell counts possible. An average logarithmic phase cell contains 1.92 × 10−14 g of deoxyribonucleic acid (DNA).

scholarly journals The Enzymatic Phosphorylation of Ribonucleic Acid and Deoxyribonucleic Acid

1966 ◽  
Vol 241 (12) ◽  
pp. 2933-2943 ◽  
Author(s):  
Abraham Novogrodsky ◽  
Moshe Tal ◽  
Abraham Traub ◽  
Jerard Hurwitz
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Enzymatic Phosphorylation
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