A RAPID METHOD FOR MEASURING ANTIBIOTIC SENSITIVITY OF BACTERIA

1965 ◽  
Vol 11 (5) ◽  
pp. 829-836 ◽  
Author(s):  
D. E. Mahony ◽  
P. Chadwick

A rapid antibiotic sensitivity test is described in which the criterion of sensitivity was the inhibition of microcolony formation on the surface of agar plates containing antibiotic. Results of the tests were available 4 hours after inoculation of the plates. Comparison between 4-hour microcolony readings and confirmatory observations after 20 hours incubation showed a discrepancy rate of 2.72%. The results obtained by the microcolony method showed a good correlation with those given by the paper disc method. Correlation with the tube dilution method was not so good, but there may be valid reasons for this. The merits and demerits of the microcolony method are discussed.

Author(s):  
Anna Joy ◽  
Aparna Anand ◽  
Arathy R Nath ◽  
Meera S Nair ◽  
Dr. K. G. Prasanth

Antibiotics are one of the most commonly prescribed drugs today. Rational use of antibiotics is therefore extremely important as their injudicious use can adversely affect the patient. Drug Utilization Evaluation (DUE) is a system of ongoing systematic criteria based evaluation of drug that will help to ensure that medicines are used appropriately. It is drug/disease specific and can be structured so that it will assess the actual process of prescribing, dispensing, or administration of drug. The retrospective study was conducted At Pk Das Institute of Medical Sciences, Palakkad, Kerala for a duration of 6 months (February 2017 - January 2018). A source of data includes Patient case sheets &medication charts, nursing charts, culture & sensitivity reports. The inclusion criterion includes Patients aged between 18- 80 year, prescribed with oral and parenteral antibiotics. . On analyzing the gender, male gender (n= 111, 55.5%) were higher in numbers as compared to female counterparts (n=89, 44.5%). In our study the majority of the patients prescribed with antibiotics were with the clinical assessment of COPD (n=39, 19.5%), UTI (n=37, 18.5%) and LRTI (n=28, 14%), Bronchial asthma (n=19, 9.5%) respectively. On analyzing the data based on antibiotic sensitivity test, antibiotic test were performed and followed in (n=64,32%)prescriptions and in (n=47,23.5%)prescriptions were test is not followed respectively. In (n=89,44.5%) prescriptions, antibiotic sensitivity test is not performed. On analysis of antibiotics prescribed, the most commonly prescribed antibiotics were cephalosporins, of these ceftriaxone was highly prescribed of all (n=95). The high percentage of antibiotic prescriptions may indicate a high probability of irrational use. This study also point out irrational use of antibiotics are more leading to resistance, misuse and serious problems. So certain strategies should be put forward to strengthen rational use of antibiotics. Keywords: Antibiotics, Antibiotic Susceptibility Test, Irrational use, Resistance


Author(s):  
Subha Ganguly

The present article reports on the bacteriological examination and antibiotic sensitivity test of pus sample collected from an abscess at the neck region of a cow.


2009 ◽  
Vol 6 (1) ◽  
pp. 114-122
Author(s):  
Baghdad Science Journal

Salmonella enteritidis one of more important as epidemiological bacteria between other salmonella types. It is very important pathologically that cause food poising and gastrointestinal tract infections. This study includes some of immunological changes that appear by ELISA test and antibiotic sensitivity test against these bacteria in mice. ELISA test results appears high immunological response happen after 3 days of inoculation, mean titration readings beginning 0.198 and the maximum mean titration after 15 days of inoculation 1.538 and begin to decrease after this time slowly to remain about 0.297 after 40 days of inoculation. An antibiotics sensitivity test result appears, this bacteria sensitive to Chloramphenicol, Ceftriaxone, Ciprofloxacin and Cotrimaxazol. Resistance to Neomycin, Streptomycin and Rifampicin, while intermediate against Ampicilin and Amoxicillin. Another test we use Vitek system to know bacterial sensitivity against to more another types of antibiotics and to confirm between some of them.


1981 ◽  
Vol 5 (5) ◽  
pp. 243-245 ◽  
Author(s):  
T. McDonald ◽  
N. Pearson ◽  
F. Johnson ◽  
F. W. O'grady

1951 ◽  
Vol 21 (9_ts) ◽  
pp. 884-891 ◽  
Author(s):  
Burton A. Wa1sbren ◽  
Claire Carr ◽  
Jean Dunnette

2020 ◽  
pp. 1-3
Author(s):  
Dunjia Wang ◽  
Dan Wang ◽  
Dunjia Wang ◽  
Heng Lyu ◽  
Hengyi Du ◽  
...  

Eight novel containing sulfur heterocyclic curcumins were synthesized and characterized by 1H-NMR, FTIR and MS spectroscopy. Their antimicrobial activities against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Aspergillus niger were also tested for MIC by using serial tube dilution method. The results showed that the antimicrobial activities of synthesized curcumin derivatives were better than curcumin. Especially, the compound 4-(1,3-dithiolan-2-ylidene)-1,7-di(thiophen-2-yl) hepta-1,6-diene-3,5- dione (2g) exhibited excellent the antimicrobial activities among these curcumin derivatives.


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