AEROBIC DEGRADATION OF CHOLINE: I. FERMENTATION OF CHOLINE BY A MARINE BACTERIUM, ACHROMOBACTER CHOLINOPHAGUM N. SP.

H. S. Shieh

doi:10.1139/m64-109

AEROBIC DEGRADATION OF CHOLINE: I. FERMENTATION OF CHOLINE BY A MARINE BACTERIUM, ACHROMOBACTER CHOLINOPHAGUM N. SP.

Canadian Journal of Microbiology ◽

10.1139/m64-109 ◽

1964 ◽

Vol 10 (6) ◽

pp. 837-842 ◽

Cited By ~ 11

Author(s):

H. S. Shieh

Keyword(s):

New Species ◽

Culture Filtrate ◽

Marine Bacterium ◽

Aerobic Degradation ◽

Animal Tissues ◽

Biochemical Characteristics ◽

Carbon And Nitrogen ◽

Enrichment Technique ◽

Substrate Feeding ◽

A New Species

A marine bacterium, isolated by an enrichment technique, is able to utilize choline as the sole carbon and nitrogen source in a medium containing various salts. The morphological and biochemical characteristics of the organism indicate that it is a new species. Substrate feeding coupled with culture filtrate analyses indicate that choline is metabolized as follows: choline → betaine → dimethylglycine + formaldehyde → sarcosine + formaldehyde → serine → pyruvate + ammonia without transmethylation being involved. This pathway has not previously been reported for other bacteria or for animal tissues.

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Isolation and identification of N2-fixing Pseudomonas associated with wetland rice

Canadian Journal of Microbiology ◽

10.1139/m83-141 ◽

1983 ◽

Vol 29 (8) ◽

pp. 867-873 ◽

Cited By ~ 43

Author(s):

W. L. Barraquio ◽

J. K. Ladha ◽

I. Watanabe

Keyword(s):

New Species ◽

Yeast Extract ◽

Heterotrophic Bacteria ◽

Fluorescent Antibody ◽

Wetland Rice ◽

Isolation And Identification ◽

Biochemical Characteristics ◽

Yeast Extract Medium ◽

Extract Medium ◽

A New Species

Semisolid yeast extract medium amended with glucose and tryptic soy agar were used to isolate aerobically N2-fixing (C2H2-reducing) heterotrophic bacteria from the root of wetland rice. The isolates were identified as Pseudomonas by gel immunodiffusion and fluorescent antibody techniques in combination with their morphological, cultural, and biochemical characteristics. The N2-fixing H2-utilizing Pseudomonas described in this paper is a new species.

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Production of a Polyunsaturated Isoprenoid Wax Ester during Aerobic Metabolism of Squalene by Marinobacter squalenivorans sp. nov

Applied and Environmental Microbiology ◽

10.1128/aem.69.7.4167-4176.2003 ◽

2003 ◽

Vol 69 (7) ◽

pp. 4167-4176 ◽

Cited By ~ 22

Author(s):

Jean-François Rontani ◽

Abdelkrim Mouzdahir ◽

Valerie Michotey ◽

Pierre Caumette ◽

Patricia Bonin

Keyword(s):

New Species ◽

Growth Phase ◽

Exponential Growth ◽

Wax Ester ◽

Ammonium Concentration ◽

Exponential Growth Phase ◽

Aerobic Degradation ◽

Ester Content ◽

Initial Cleavage ◽

A New Species

ABSTRACT This paper describes the production of 5,9,13-trimethyltetradeca-4E,8E,12-trienyl-5,9,13-trimethyltetradeca-4E,8E,12-trienoate during the aerobic degradation of squalene by a Marinobacter strain, 2Asq64, isolated from the marine environment. A pathway involving initial cleavage of the C10-C11 or C14-C15 double bonds of the squalene molecule is proposed to explain the formation of this polyunsaturated isoprenoid wax ester. The isoprenoid wax ester content reached 1.1% of the degraded squalene at the mid-exponential growth phase and then decreased during the stationary phase. The wax ester content increased by approximately threefold in N-limited cultures, in which the ammonium concentration corresponds to conditions often found in marine sediments. This suggests that the bacterial formation of isoprenoid wax esters might be favored in such environments. The bacterial strain is then characterized as a member of a new species, for which we propose the name Marinobacter squalenivorans sp. nov.

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Characteristics of Psychrotrophic Clostridium laramie Causing Spoilage of Vacuum-packaged Refrigerated Fresh and Roasted Beef

Journal of Food Protection ◽

10.4315/0362-028x-56.1.13 ◽

1993 ◽

Vol 56 (1) ◽

pp. 13-17 ◽

Cited By ~ 59

Author(s):

N. KALCHAYANAND ◽

B. RAY ◽

R. A. FIELD

Keyword(s):

New Species ◽

Fatty Acid ◽

Pure Culture ◽

Soluble Protein ◽

Volatile Fatty Acid ◽

Protein Profiles ◽

Biochemical Characteristics ◽

Clostridial Species ◽

A New Species ◽

Clostridium Spp

A Clostridium sp., associated with an unusual spoilage of vacuum-packaged refrigerated uncooked (fresh) and roasted beef, was isolated as a pure culture from the spores in spoiled beef. The pure culture was used to study important characteristics and to differentiate it from other clostridial species usually found in beef. The biochemical characteristics, volatile fatty acid and soluble-protein profiles, temperatures and pHs of growth, and temperatures of sporulation, germination, and destruction of the isolate were distinctly different from other Clostridium spp. studied. It is not toxigenic and has 26% guanine plus cytosine. The isolate is a new species and is named Clostridium laramie.

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Marine Bacterium Vibrio sp. CB1-14 Produces Guanidine Alkaloid 6-epi-Monanchorin, Previously Isolated from Marine Polychaete and Sponges

Marine Drugs ◽

10.3390/md17040213 ◽

2019 ◽

Vol 17 (4) ◽

pp. 213 ◽

Cited By ~ 1

Author(s):

Tatyana Makarieva ◽

Larisa Shubina ◽

Valeria Kurilenko ◽

Marina Isaeva ◽

Nadezhda Chernysheva ◽

...

Keyword(s):

New Species ◽

Marine Bacterium ◽

Culture Broth ◽

Rrna Gene ◽

Bacterial Strains ◽

Genome Sequences ◽

16S Rrna Gene Analysis ◽

Chaetopterus Variopedatus ◽

Marine Polychaete ◽

A New Species

Twenty-three bacterial strains were isolated from the secreted mucus trapping net of themarine polychaete Chaetopterus variopedatus (phylum Annelida) and twenty strains were identifiedusing 16S rRNA gene analysis. Strain CB1-14 was recognized as a new species of the genus Vibriousing the eight-gene multilocus sequence analysis (MLSA) and genome sequences of nineteen typeVibrio strains. This Vibrio sp. was cultured, and 6-epi-monanchorin (2), previously isolated from thepolychaete and two sponge species, was found in the cells and culture broth. The presence of the 6-epi-monanchorin was confirmed by its isolation followed by 1H NMR and HRESIMS analysis. Theseresults showed the microbial origin of the bicyclic guanidine alkaloid 2 in C. variopedatus.

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