SOME CHARACTERISTICS OF PROTEINASES OF AN OBLIGATELY PSYCHROPHILIC RED-PIGMENTED BACTERIUM AND OF SERRATIA MARCESCENS

1963 ◽  
Vol 9 (6) ◽  
pp. 871-877 ◽  
Author(s):  
I. J. Mcdonald ◽  
Alice K. Chambers

Supernatants of cultures of a red-pigmented psychrophilic bacterium and of a strain of Serratia marcescens contained proteolytic enzymes that hydrolyzed casein,/3-IactogIobuIin and haemoglobin. With casein as the substrate, optimum activity of the enzyme system of the psychrophile was at pH 10 and at 40-45 °C; optimum activity of the 5. marcescens enzyme system was at pH 9.0 and 45° C. Calcium stimulated activity and protected proteinases of both organisms against heat inactivation. Proteinases of both organisms possess high temperature characteristics and relatively high temperature optima. The results indicated that the proteinase system of the red psychrophile differs from those of some other cold-tolerant bacteria {Cytophaga and Pseudomonas) and that it is similar to but not identical with the proteinase system of Serratia marcescens.

2012 ◽  
Vol 31 (1) ◽  
pp. 11105 ◽  
Author(s):  
Puja Gawas-Sakhalkar ◽  
ShivMohan Singh ◽  
Simantini Naik ◽  
Rasik Ravindra

2021 ◽  
Vol 68 (4) ◽  
pp. 2025-2032
Author(s):  
Jingcun Liu ◽  
Ming Xiao ◽  
Ruizhe Zhang ◽  
Subhash Pidaparthi ◽  
Hao Cui ◽  
...  

2016 ◽  
Vol 2016 (CICMT) ◽  
pp. 000053-000057
Author(s):  
Jaroslaw Kita ◽  
Sven Wiegärtner ◽  
Alistair Prince ◽  
Peter Weigand ◽  
Ralf Moos

Abstract The application of thermocouples as temperature sensors has been well known and has already been established for many years. However, for classical thick-film technology using screen-printing and firing, no standardized solutions exist. The here-presented newly developed PtRh thick-film compositions (90% Pt,10% Rh) allows to construct thick-film type S thermocouples (Pt/PtRh), following the IEC temperature characteristics. They can be fired in air, and therefore can be easily integrated into existing thick-film components and devices. In an earlier study, the new Pt-Rh composition was successfully tested on alumina substrates. Their electrical characteristics is equal with classical wire type S thermocouples. This study continues the investigations of thick-film thermocouples. We tested the newly developed pastes for high temperature applications on alumina substrates and evaluated the application of the new screen-printable type S thermocouples on LTCC ceramics. Three possible configurations were investigated: deposited on already fired LTCC substrates (post-fired), screen-printed and co-fired with LTCC tapes on the top surface as well as as buried structures. The paper presents the results of our evaluation and discusses further possible applications.


2003 ◽  
Vol 71 (8) ◽  
pp. 686-690 ◽  
Author(s):  
Katsuhiko SHINYAMA ◽  
Yoshifumi MAGARI ◽  
Atsuhiro FUNAHASHI ◽  
Toshiyuki NOHMA ◽  
Ikuo YONEZU

2019 ◽  
Vol 116 (2) ◽  
pp. 211
Author(s):  
Shengli Wu ◽  
Xiaobo Zhai ◽  
Tiankai Song

A sintering burden blending model is an intelligent system used to obtain the optimal blending proportions of burdens with minimal sintering burden cost. In this study, micro-sintering and sinter pot tests were first carried out to clarify the quantitative relationship between the shatter index (SI) of the sinter and high-temperature characteristics (HTCs) of the ore blends. The result shows that the lowest assimilation temperature (LAT) plays a dual role in SI, whereas the index of liquid phase fluidity (ILF) and compressive strength of the bonding phase (CSB) have positive effects on SI. The effect of the ILF is the largest. Based on the one-step optimization method, suitable ranges of room-temperature characteristics (RTCs) of ore blends, obtained relationship between sinter strength and HTCs of ore blends, sintering theory, and bisection and simplex algorithms, the proposed sintering burden blending model is established. The validation for the model shows that it is effective at utilizing iron ore resources, maintaining high strength of the sinter, while reducing burden costs.


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