INHIBITION OF FUNGAL GROWTH BY BACTERIA DURING CELLULOSE-DECOMPOSITION

1961 ◽  
Vol 7 (6) ◽  
pp. 857-863 ◽  
Author(s):  
Y. Henis ◽  
Paulina Keller ◽  
A. Keynan
Keyword(s):  
Bacterial Growth ◽  
Filter Paper ◽  
Vegetative Growth ◽  
Pure Culture ◽  
Carboxymethyl Cellulose ◽  
Agar Medium ◽  
Fungal Growth ◽  
Fungal Development ◽  
Cellulose Decomposition ◽  
Cellulose Filter

The cellulose-decomposing flora developing from soil crumbs on filter paper placed upon an agar medium was studied. Under these conditions fungal development was significantly inhibited by bacterial growth. When cellophane was substituted for filter paper, no inhibition of fungal growth was observed. A pure culture of Cellvibrio inhibited the vegetative growth of Stachybotrys when inoculated simultaneously on media containing carboxymethyl-cellulose, filter paper, or glucose up to 0.1%. This inhibition was shown to be caused by competition for available carbohydrates.

scholarly journals Substrate specificity and mode of action of the cellulases from the thermophilic fungus Thermoascus aurantiacus

1981 ◽  
Vol 193 (1) ◽  
pp. 67-74 ◽  
Author(s):  
M G Shepherd ◽  
C C Tong ◽  
A L Cole
Keyword(s):  
Filter Paper ◽  
Mode Of Action ◽  
Carboxymethyl Cellulose ◽  
Xylanase Activity ◽  
Rapid Decrease ◽  
Thermoascus Aurantiacus ◽  
Inherent Feature ◽  
Rate Of Hydrolysis ◽  
Beta Glucosidase ◽  
Cellulose Filter

The substrate specificities of three cellulases and a beta-glucosidase purified from Thermoascus aurantiacus were examined. All three cellulases partially degraded native cellulose. Cellulase I, but not cellulase II and cellulase III, readily hydrolyzed the mixed beta-1,3; beta-1,6-polysaccharides such as carboxymethyl-pachyman, yeast glucan and laminarin. Both cellulase I and the beta-glucosidase degraded xylan, and it is proposed that the xylanase activity is an inherent feature of these two enzymes. Lichenin (beta-1,4; beta-1,3) was degraded by all three cellulases. Cellulase II cannot degrade carboxymethyl-cellulose, and with filter paper as substrate the end product was cellobiose, which indicates that cellulase II is an exo-beta-1,4-glucan cellobiosylhydrolase. Degradation of cellulose (filter paper) can be catalysed independently by each of the three cellulases; there was no synergistic effect between any of the cellulases, and cellobiose was the principal product of degradation. The mode of action of one cellulase (cellulase III) was examined by using reduced cellulodextrins. The central linkages of the cellulodextrins were the preferred points of cleavage, which, with the rapid decrease in viscosity of carboxymethyl-cellulose, confirmed that cellulase III was an endocellulase. The rate of hydrolysis increased with chain length of the reduced cellulodextrins, and these kinetic data indicated that the specificity region of cellulase III was five or six glucose units in length.

scholarly journals Well-Dispersed Silver Nanoparticles on Cellulose Filter Paper for Bacterial Removal

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 595
Author(s):  
Hsiu-Wen Chien ◽  
Ming-Yen Tsai ◽  
Chia-Jung Kuo ◽  
Ching-Lo Lin
Keyword(s):  
Silver Nanoparticles ◽  
Filter Paper ◽  
X Ray Diffraction ◽  
Bacterial Reduction ◽  
Treatment Technology ◽  
Bacterial Removal ◽  
Contaminated Drinking Water ◽  
Gravity Driven ◽  
Filter Papers ◽  
Cellulose Filter

In this study, a polydopamine (PDA) and polyethyleneimine (PEI)-assisted approach was developed to generate well-distributed PDA/PEI/silver (PDA/PEI/Ag) nanocomplexes on the surfaces of commercial cellulose filter papers to achieve substantial bacterial reduction under gravity-driven filtration. PDA can bind to cellulose paper and act as a reducer to produce silver nanoparticles (AgNPs), while PEI can react with oxidative dopamine and act as a dispersant to avoid the aggregation of AgNPs. The successful immobilization of PDA/PEI/Ag nanocomplexes was confirmed by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) were used as pathogen models to test the efficacy of the PDA/PEI/Ag nanocomplex-incorporated filter papers. The PDA/PEI/Ag nanocomplex-incorporated filter papers provided a substantial bacterial removal of up to 99% by simple gravity filtration. This work may be useful to develop a feasible industrial production process for the integration of biocidal AgNPs into cellulose filter paper and is recommended as a local-condition water-treatment technology to treat microbial-contaminated drinking water.

scholarly journals Robust immobilization of anionic silver nanoparticles on cellulose filter paper toward a low-cost point-of-use water disinfection system with improved anti-biofouling properties

RSC Advances ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 4873-4882
Author(s):  
Gongyan Liu ◽  
Ruiquan Yu ◽  
Jing Jiang ◽  
Zhuang Ding ◽  
Jing Ma ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Filter Paper ◽  
Low Cost ◽  
Water Disinfection ◽  
Point Of Use ◽  
Cellulose Filter

Point-of-use water disinfection by GA@AgNPs-LA-FP.

Imidazolium functionalized cellulose filter paper derived from waste newspaper and its application in removal of chromium(VI)

2020 ◽  
Vol 157 ◽  
pp. 104776
Author(s):  
Wenqi Song ◽  
Miaoxiu Yang ◽  
Yuzhen Zhao ◽  
Min Zhu ◽  
Yanfang Zhu ◽  
...  
Keyword(s):  
Filter Paper ◽  
Chromium Vi ◽  
Cellulose Filter

scholarly journals In Vitro Seed Germination and Embryo Culture in Nothapodytes foetida (Wight) Sleumer

2015 ◽  
Vol 48 ◽  
pp. 23-31 ◽  
Author(s):  
S. Kaveri ◽  
Rao Srinath
Keyword(s):  
Seed Germination ◽  
Embryo Culture ◽  
Filter Paper ◽  
Agar Medium ◽  
Ms Medium ◽  
Mature Embryos ◽  
Nothapodytes Foetida ◽  
Half Strength ◽  
Paper Bridge

In vitro seed germination and embryo culture have been achieved in Nothapodytes foetida, this plant is known for its rich source of anticancer drug i. e., Camptothecin. In present study both normal and decoated seeds were subjected to different treatments viz., H2O, GA3, H2O2, H2SO4, chlorine water and mechanical scarification, further these were germinated on water agar medium (WA), filter paper bridge (FB), half strength MS (HMS) and full strength MS (FMS) medium. The highest percentage (69%) of germination was achieved from decoated seeds treated with 10mg/L GA3 and germinated on Filter Paper Bridge. And for embryo culture mature embryos were inoculated on MS medium containing various combination and concentrations of cytokinins (BAP, Kn and TDZ) and auxin (IAA and NAA) for rapid conversion into a plantlet. Among the different combinations of growth regulators; highest frequency (100%) of plantlet conversion was obtained on MS medium containing Kn (1.0mg/L) and NAA (0.2mg/L).

scholarly journals Fibrous porous carbon electrocatalysts for hydrazine oxidation by using cellulose filter paper as precursor and self-template

Carbon ◽  
2016 ◽  
Vol 102 ◽  
pp. 97-105 ◽  
Author(s):  
Alessandro C. Martins ◽  
Xiaoxi Huang ◽  
Anandarup Goswami ◽  
Katherine Koh ◽  
Yuying Meng ◽  
...  
Keyword(s):  
Filter Paper ◽  
Porous Carbon ◽  
Hydrazine Oxidation ◽  
Cellulose Filter

scholarly journals Influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina

2013 ◽  
Vol 39 (2) ◽  
pp. 126-129 ◽  
Author(s):  
Diana Erica Gómez ◽  
Erlei Melo Reis
Keyword(s):  
Filter Paper ◽  
Agar Medium ◽  
Soybean Seed ◽  
Light Filter ◽  
Seed Extract ◽  
Cercospora Sojina ◽  
Light Regimes ◽  
Extract Agar ◽  
Tomato Extract ◽  
The Media

Fungi require special substrates for their isolation, vegetative growth and sporulation. In experiments conducted in the laboratory, the influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina conidia, the causal agent of soybean frogeye leaf spot, was assessed. The media potato sucrose agar, V-8 agar, tomato extract agar, soybean leaf extract agar, soybean seed extract agar, soybean meal agar, soybean flour agar and wheat flour agar were tested, added on the surface, with and without filter paper and under two light regimes, with 12 h light at 25°± 2°C and in the dark. A triple factorial 8x2x2 (substrates x light/dark x with/without filter paper) design with four replicates was used. V-8 agar medium was employed and the pH was adjusted with HCl 0.1N or NaOH 0.1N before autoclaving to the values: 3, 4, 5, 6, 7 and 8, and the pH of V-8 agar medium is 6.7. The evaluation was done on the seventh day of incubation. Data underwent regression analysis. Sporulation was maximized on the agar media V-8, seed extract, oat flour, tomato extract, and potato sucrose in the presence of filter paper and 12h light. On V-8 medium, maximal sporulation was obtained with pH 6.7.

Multiplication Characteristics of FRNA Phage and Its Utility as an Indicator for Pathogenic Viruses

1993 ◽  
Vol 27 (3-4) ◽  
pp. 133-136 ◽  
Author(s):  
Naoyuki Kamiko ◽  
Shinichiro Ohgaki
Keyword(s):  
Bacterial Growth ◽  
Growth Phase ◽  
Substrate Concentration ◽  
Pure Culture ◽  
Environmental Samples ◽  
Cultivation Temperature ◽  
Pathogenic Viruses ◽  
Phage Multiplication ◽  
Rna Phage

The possibility of multiplication of F-specific RNA phage (FRNA phage) multiplication in the environment was investigated. Using the Qβ strain in pure culture as a model FRNA phage, the effects of bacterial growth phase, substrate concentration and cultivation temperature on phage multiplication were studied. Similar experiments with environmental samples from raw sewage were then preformed.

scholarly journals Optimization of Agitation Rate in Bioreactor Increases Chitinase Activity of Serratia marcescens PT6

2020 ◽  
Vol 147 ◽  
pp. 03019
Author(s):  
Amara Faiz Wriahusna ◽  
Niswah Umhudloh Dzakiyya ◽  
Indun Dewi Puspita ◽  
Sri Pudjiraharti
Keyword(s):  
Serratia Marcescens ◽  
Bacterial Growth ◽  
Agar Medium ◽  
Chitinase Activity ◽  
Colloidal Chitin ◽  
Gram Negative Bacteria ◽  
Agitation Rate ◽  
Anova Analysis ◽  
Gram Negative ◽  
Shrimp Pond

Serratia marcescens PT6 is a Gram-negative bacteria isolated from shrimp pond sediment that capable of producing chitinase. This study aimed to observe the effect of agitation rate on growth and chitinase activity of S. marcescens PT-6 in a bioreactor. The production of chitinase was done in 1.5 l bioreactor using colloidal chitin broth at the condition of pH 7, the temperature of 30°C, aeration of 0.04 vvm, and variation of agitation rate (200, 350, 500 rpm). Bacterial growth was measured by colonies counting in agar medium, while chitinase activity was measured by means of colorimetric every day for four days incubation. The results of ANOVA analysis show that the agitation rate had no effect on bacterial growth, but a significant effect (P<0.05) was observed on chitinase activity. The highest growth and chitinase activity were obtained at 200 rpm, with the highest chitinase activity of 0.006 ± 0.001 U/ml was at day-2. This study implies that the optimized agitation rate in the bioreactor increased the chitinase activity produced by S. marcescens PT-6.

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