A METHANOL-UTILIZING BACTERIUM: II. STUDIES ON THE PATHWAY OF METHANOL ASSIMILATION

1959 ◽  
Vol 5 (2) ◽  
pp. 187-195 ◽  
Author(s):  
T. Kaneda ◽  
J. M. Roxburgh
Keyword(s):  
Dehydrogenase Activity ◽  
The Other ◽  
Formaldehyde Dehydrogenase ◽  
Pseudomonas Sp ◽  
Carbonate Carbon ◽  
Oxidation Of Methanol ◽  
Carbon Compounds

Two simultaneous pathways for the fixation of carbon from one-carbon compounds by a Pseudomonas sp. (PRL-W4) are postulated. Carbonate and formate form parts of one pathway and methanol and formaldehyde the other. It is presumed that both the formate and formaldehyde are incorporated as serine by the organism. The energy necessary for the incorporation of carbonate carbon can be supplied by the oxidation of methanol or of formate but not formaldehyde. No formaldehyde dehydrogenase activity was found.

Regulation of branched-chain 2-oxo acid dehydrogenase activity during exercise

1989 ◽  
Vol 256 (1) ◽  
pp. E186-E190 ◽  
Author(s):  
G. J. Kasperek
Keyword(s):  
Dehydrogenase Activity ◽  
Plasma Levels ◽  
Recovery Period ◽  
The Other ◽  
Atp Content ◽  
Excellent Correlation ◽  
Atp Level ◽  
Control Value ◽  
Acid Dehydrogenase ◽  
Branched Chain

The levels of the branched-chain amino and oxo acids were measured in muscle and plasma after exercise and 10 min postexercise. Leucine was increased in both muscle and plasma after exercise and 10 min postexercise. The muscle levels of the branched-chain oxo acids were not increased immediately after exercise but were increased 10 min postexercise. Exercise caused a large increase in the plasma levels of the oxo acids of leucine and isoleucine that was further increased 10 min postexercise. The activity of branched-chain 2-oxo acid dehydrogenase (BCOAD) was increased immediately after exercise but returned to the control value by 10 min postexercise. The lack of correlation between the muscle and plasma levels of the branched-chain amino and oxo acids and BCOAD activity suggests that these amino and oxo acids are not the primary physiological regulators of BCOAD activity during exercise. On the other hand, an excellent correlation was found between the muscle ATP level and BCOAD activity, with the ATP content decreasing in tandem with an increase in BCOAD activity during exercise and decreasing during the recovery period after exercise.

scholarly journals Regulation of the enzymes converting l-mandelate into benzoate in bacterium N.C.I.B. 8250

1972 ◽  
Vol 130 (4) ◽  
pp. 937-946 ◽  
Author(s):  
A. Livingstone ◽  
C. A. Fewson
Keyword(s):  
Alcohol Dehydrogenase ◽  
Single Cell ◽  
Benzyl Alcohol ◽  
Dehydrogenase Activity ◽  
The Other ◽  
Cell Free Extract ◽  
Mutant Strains ◽  
Benzaldehyde Dehydrogenase ◽  
Kinetics Of

l-Mandelate is oxidized to benzoate by the enzymes l-mandelate dehydrogenase, phenylglyoxylate carboxy-lyase and benzaldehyde dehydrogenase I. Conditions have been established for measuring these three enzymes as well as benzyl alcohol dehydrogenase, benzaldehyde dehydrogenase II and catechol 1,2-oxygenase in a single cell-free extract prepared from bacterium N.C.I.B. 8250. The kinetics of induction of all these enzymes have been measured under a variety of conditions. l-Mandelate dehydrogenase, phenylglyoxylate carboxy-lyase and benzaldehyde dehydrogenase I appear to be co-ordinately regulated because (a) their differential rates of synthesis are proportional to one another under various conditions of induction and repression, (b) they are specifically and gratuitously induced by thiophenoxyacetate and a number of other compounds, and (c) mutant strains have been isolated that lack all three enzymes. Phenylglyoxylate is the primary inducer of the regulon as mutant strains lacking phenylglyoxylate carboxy-lyase form the other two enzymes in the presence of l-mandelate or phenylglyoxylate, whereas in mutant strains devoid of l-mandelate dehydrogenase activity only phenylglyoxylate induces phenylglyoxylate carboxy-lyase and benzaldehyde dehydrogenase I.

scholarly journals INDUCED DEGRADATION OF CRUDE OIL POLLUTED SOIL BY MICROBIAL AUGMENTATION

2017 ◽  
Vol 23 (4) ◽  
pp. 37-44
Author(s):  
SYLVESTER UWADIAE ◽  
EMIKE OMOAYENA
Keyword(s):  
Crude Oil ◽  
Soil Sample ◽  
Contaminated Soil ◽  
Polluted Soil ◽  
Soil Samples ◽  
The Other ◽  
Pseudomonas Sp ◽  
Hydrocarbon Content ◽  
Percentage Removal

This study was aimed at assessing the effectiveness of indigenous microbes for remediation of hydrocarbon contaminated soil by first increasing the population of the indigenous microbes via bioaugmentation. Soil samples were treated using a consortium of bacteria: Bacillus substilis and Pseudomonas sp. which were isolated and cultured from the contaminated soil. The non-bacteria injected soil sample had the highest hydrocarbon content (THC) of 271.021 in comparison with the other soil samples. The THC percentage removal of B1 (96.885 %), B2 (97.562 %), B3 (98.835 %), B4 (99.594 %) and B5 (99.540 %) were higher than that of the control; indicating that biodegradation actually took place.

scholarly journals Characterization of the glucose 6-phosphate dehydrogenase activity in rat liver mitochondria

1976 ◽  
Vol 159 (3) ◽  
pp. 683-687 ◽  
Author(s):  
M Grunwald ◽  
H Z Hill
Keyword(s):  
Dehydrogenase Activity ◽  
Rat Liver ◽  
Liver Mitochondria ◽  
The Other ◽  
Rat Liver Mitochondria ◽  
Kinetic Behaviour ◽  
Two Peaks ◽  
Glucose 6 Phosphate Dehydrogenase

Glucose 6-phosphate dehydrogenase activity in rat liver mitochondria can be released by detergent. The released activity is separated by chromatography into two peaks. One peak has the kinetic behaviour and mobility similar to the soluble sex-linked enzyme, whereas the other peak is similar to the microsomal hexose 6-phosphate dehydrogenase. There is no evidence for the existence of a new glucose 6-phosphate dehydrogenase activity in rat liver mitochondria.

scholarly journals Uji In Vitro Gabungan Ekstrak Boesenbergia pandurata, Solanum ferox, Zingimber zerumbet terhadap Bakteri Patogen pada Ikan Nila (IN VITRO TEST OF CONCOCTION PLANT EXTRACTS OF BOESENBERGIA PANDURATA, SOLANUM FEROX, ZINGIMBER ZERUMBET

2018 ◽  
Vol 19 (1) ◽  
pp. 35
Author(s):  
Esti Handayani Hardi ◽  
Gina Saptiani ◽  
Nurkadina Nurkadina ◽  
Irawan Wijaya Kusuma ◽  
Wiwin Suwinarti
Keyword(s):  
Antibacterial Activity ◽  
Plant Extracts ◽  
Aeromonas Hydrophila ◽  
Inhibition Zone ◽  
The Other ◽  
In Vitro Test ◽  
Pseudomonas Sp ◽  
Vitro Test

The aim of this study was to evaluate the antibacterial activity of ethanol concoction (mixed extract) of three herbs plants, such as: Boesenbergia pandurata, Solanum ferox and Zingimber zerumbet against Aeromonas hydrophila, Pseudomonas sp. and both bacteria. The Plant extracts were obtained by using ethanol and the concentration of the extract was 600 mgL-1 of B. pandurata, 900 mgL-1 of S. ferox and 200 mgL-1 of Z. zerumbet. The inhibition zone was measured after 6, 12, 18, 24 h incubation at 30o C. The ratios of concoction S. ferox and B. pandurata (for 100 mL solution) were 90:10; 80:20; 70:30; 60:40; 50:50; 40:60; 30:70; 20:80; 10:90. The same ratios were made on the concoction of S. ferox and Z. zerumbet. The concoctions of S. ferox and B. pandurata in the ratio of 50:50 and 60:40, and the concoction of S. ferox and Z. Zerumbet with ratio 60:40 had higher antibacterial activity against A. hydrophila single isolate compared to the other concoctions. Furthermore, the concoction extract of S. ferox and B. pandurate 50:50 and the combined of S. ferox and Z. zerumbet ratio 50:50 and 90:10 were the best combination to inhibit the growth of a single bacterium Pseudomonas sp. The combined S. ferox and B. pandurata ratio 50:50; 10:90 and S. ferox mixed with Z. zerumbet ratio 50:50 and 40:60 were the best combination against the combined bacteria between A. hydrophila and Pseudomonas sp. The conclusion of this research was the combined extract of S. ferox and B. pandurata and S. ferox with Z. zerumbet are effective to suppress the growth of single or combination of A. hydropila and Pseudomonas sp.

scholarly journals Spectrum of the flame of ethylene

1934 ◽  
Vol 147 (862) ◽  
pp. 513-521 ◽  
Keyword(s):  
Ultra Violet ◽  
Detailed Examination ◽  
Outer Tube ◽  
The Other ◽  
Bunsen Flame ◽  
Ethylene Flame ◽  
Carbon Compounds ◽  
Pass Through ◽  
Gas Supply

In the course of a detailed examination of the spectra of the flames of carbon compounds, which was undertaken at the suggestion of Professor A. Fowler, it has been found that the flame of ethylene shows, in addition to the familiar bands of C 2 , CH and HO, a system of bands which has not previously been adequately described. These bands occur feebly in the ordinary Bunsen flame, as noted by Rassweiler and Withrow, and with greater intensity in the flame of ether, as shown in a photograph by Emeléus. Other flames which exhibit these bands have also been found in the course of the present investigation, but as the bands appear to have their greatest intensity in ethylene, they may conveniently be called the “ethylene flame bands” for the purposes of the present paper. The bands are degraded to the red, and have been observed to extend from λ 4100 to λ 2500. For the production of the bands under the most favourable conditions it was necessary to use a Smithells’ flame separator to divide the inner and outer cones. The separator employed, fig. 1, considered to two concentric tubes, F and G, the inner tube, F, being mounted on the outer nozzle, D, of a two-way blow-pipe. The inner tube was made of Pyrex, 15cm in length and 1·2 cm in diameter, while the outer one—12 cm long and 1·5 cm in diameter—was made of quartz so as to permit observations in the ultra-violet. The inlet A was connected to a cylinder of ethylene gas (rated 100% pure), the arrangement being such that the gas could then pass through the outer nozzle, D. Through the inner nozzle, E, could be passed air blown through the other inlet, B, by an electric blower. In the absence of air, the gas burnt with a smoky flame on the outer tube, G. When air was blown through, the flame became non-luminous and, with suitable adjustment of air and gas supply by the handle, C, the inner cone was clearly developed. Then, on sliding the inner tube of the separator about 4 cm below the top of the outer tube, the cones were completely separated, the inner cone burning steadily on the inner tube. Under these conditions the inner cone was about 2 cm high, and was of greenish colour.

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