ANTIGENIC ANALYSIS OF DISSOCIANTS AND SEROLOGICAL TYPES OF PASTEURELLA MULTOCIDA

1957 ◽  
Vol 3 (7) ◽  
pp. 1021-1029 ◽  
Author(s):  
Sheldon A. London ◽  
Katherine E. Yaw
Keyword(s):  
Pasteurella Multocida ◽  
Antigenic Structure ◽  
Immunological Methods ◽  
Antigenic Analysis ◽  
Dissociation Pattern ◽  
Somatic Antigens ◽  
Type Strains

Pasteurella multocida has been separated into serological types on the basis of a variety of immunological methods by earlier workers. A definite dissociation pattern has also been described. This study presents evidence that two colonial variants of a type 1 strain are qualitatively identical in their antigenic structure but differ quantitatively with regard to one antigen. The data also indicate the similarity of somatic antigens of three type strains though antigenic differences can be demonstrated in the Jennings triangle plate which possibly represent differences in the substances composing the capsule.

scholarly journals ANTIGENIC ANALYSIS OF CERTAIN GROUP B ARTHROPOD-BORNE VIRUSES BY ANTIBODY ABSORPTION

1960 ◽  
Vol 111 (1) ◽  
pp. 21-32 ◽  
Author(s):  
D. H. Clarke
Keyword(s):  
Yellow Fever ◽  
Vaccine Strain ◽  
Yellow Fever Virus ◽  
Geographical Area ◽  
Specific Antigen ◽  
Antigenic Structure ◽  
Antigenic Analysis ◽  
West Nile ◽  
Antibody Absorption ◽  
Group B

A method for carrying out antibody absorption studies for antigenic analysis of group B arthropod-borne (arbor) viruses is described and examples of homologous and heterologous absorption curves are presented. Evidence that antigenic structure can be a stable property was obtained with three strains of West Nile virus isolated from different hosts in different countries over a period of years. Comparative studies with viruses of the Japanese B-St. Louis-West Nile subgroup indicate that each virus contains a completely specific antigen as well as one or more cross-reactive components. Strains of yellow fever virus isolated in America were shown to lack an antigen present in strains of African origin although no differences were found between isolates from the same geographical area. The attenuated 17 D vaccine strain of yellow fever was found to have acquired an additional antigen not present in the unadapted parent or in other strains tested. However, alteration in pathogenicity for man was not found to be necessarily attended by any antigenic modification, as shown by the antigenic identity of the French neurotropic vaccine strain with its pantropic parent.

scholarly journals Characterisation of avian Pasteurella multocida strains with PCR-RFLP analysis of the ompH gene

2013 ◽  
Vol 61 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Boglárka Sellyei ◽  
Éva Ivanics ◽  
Tibor Magyar
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Pasteurella Multocida ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
The Other ◽  
Pcr Rflp ◽  
H Gene ◽  
Geographic Regions ◽  
Type Strains ◽  
Restriction Fragment Length

The 16 somatic serotype type strains and 60 field isolates of Pasteurella multocida, representing various avian species and geographic regions in Hungary, were characterised by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the ompH gene with DraI restriction endonuclease. The type strains yielded eight different (I-VIII) profiles. Strains whose PCR fragment was uncut by DraI (profile IV) could be differentiated with HindIII and PvuII restriction endonucleases. Five of the eight PCR-RFLP profiles (I, III, V, VI and VII) were detected among the field strains. Only a correlation of limited strength was found between the classical somatic serotypes and the PCR-RFLP profiles. However, the results confirmed that molecular methods could confidently distinguish serotype A:1 strains from the other serotypes. Moreover, the specific relationship between somatic serotypes and PCR-RFLP types among isolates from turkey raises the possibility of the existence of host-specific clones within the P. multocida population.

Diagnostic accuracy of four different immunological methods for the detection of anti-F-actin autoantibodies in type 1 autoimmune hepatitis and other liver-related disorders

Autoimmunity ◽  
2008 ◽  
Vol 41 (1) ◽  
pp. 105-110 ◽  
Author(s):  
Danilo Villalta ◽  
Nicola Bizzaro ◽  
Mirella Da Re ◽  
Renato Tozzoli ◽  
Lars Komorowski ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Autoimmune Hepatitis ◽  
Immunological Methods

scholarly journals Glycosaminoglycan-Binding Ability Is a Feature of Wild-Type Strains of Herpes Simplex Virus Type 1

Virology ◽  
2002 ◽  
Vol 302 (2) ◽  
pp. 413-419 ◽  
Author(s):  
Edward Trybala ◽  
Anette Roth ◽  
Maria Johansson ◽  
Jan-Åke Liljeqvist ◽  
Elham Rekabdar ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Wild Type ◽  
Binding Ability ◽  
Simplex Virus ◽  
Type Strains

scholarly journals A retrospective study on the presence of selected infectious agents in lung samples of cats with pneumonia

2020 ◽  
Author(s):  
Eva Schmal-Filius ◽  
Nora Nedorost ◽  
Christiane Weissenbacher-Lang ◽  
Herbert Weissenböck
Keyword(s):  
Retrospective Study ◽  
Canine Distemper Virus ◽  
Pasteurella Multocida ◽  
Infectious Agents ◽  
Canine Distemper ◽  
Herpesvirus Type ◽  
Feline Herpesvirus ◽  
Feline Coronavirus ◽  
Feline Herpesvirus Type 1

AbstractThe causative role of some infectious agents found in cases of feline pneumonia is under debate, because they are also part of the physiological microbiota of the respiratory tract of healthy animals. In this retrospective study, archived formalin-fixed and paraffin-wax-embedded lung samples of 69 severe and lethal cases of pneumonia in cats were examined by immunohistochemistry (IHC) for the detection of nine selected infectious agents: Pasteurella multocida, Bordetella bronchiseptica, Mycoplasma felis, M. gateae, Chlamydia felis, feline herpesvirus type 1, feline coronavirus, canine distemper virus, and Toxoplasma gondii. The intention was to elucidate their immediate involvement in pneumonia formation. Due to the cross-reactivity of the applied antibodies, a species-specific polymerase chain reaction (PCR) for both targeted Mycoplasma species was applied additionally. In the 42 cases (60.9%) positive for at least one pathogen, several agents were present in a high proportion of the samples (P. multocida – 34.8%, B. bronchiseptica – 29.0%), while others were present in a moderate (feline herpesvirus type 1 – 18.8%, M. gateae – 13.0%, M. felis – 10.1%) or low percentage (T. gondii – 1.4%). All samples were negative for C. felis, feline coronavirus and canine distemper virus. Mixed infections of up to four pathogens were more frequent than single infections. Mycoplasma preferably colonised lung tissue damaged by other pathogens because they never occurred as single infections. Pasteurella multocida, B. bronchiseptica, M. felis, feline herpesvirus type 1 and T. gondii showed abundant replication within lung lesions, thus suggesting a prominent role in pneumonia formation.

scholarly journals Antigenic and Molecular Characterization of Wild Type 1 Poliovirus Causing Outbreaks of Poliomyelitis in Albania and Neighboring Countries in 1996

1998 ◽  
Vol 36 (7) ◽  
pp. 1912-1918 ◽  
Author(s):  
L. Fiore ◽  
D. Genovese ◽  
E. Diamanti ◽  
S. Catone ◽  
B. Ridolfi ◽  
...  
Keyword(s):  
Western Europe ◽  
Antigenic Structure ◽  
Wild Type ◽  
Wild Poliovirus ◽  
The Past ◽  
The World ◽  
Large Outbreak ◽  
Human Sera

Mass vaccination has led poliomyelitis to become a rare disease in a large part of the world, including Western Europe. However, in the past 20 years wild polioviruses imported from countries where polio is endemic have been responsible for outbreaks in otherwise polio-free European countries. We report on the characterization of poliovirus isolates from a large outbreak of poliomyelitis that occurred in Albania in 1996 and that also spread to the neighboring countries of Yugoslavia and Greece. The epidemics involved 145 subjects, mostly young adults, and caused persisting paralysis in 87 individuals and 16 deaths. The agent responsible for the outbreak was isolated from 74 patients and was identified as wild type 1 poliovirus by both immunological and molecular methods. Sequence analysis of the genome demonstrated the involvement of a single virus strain throughout the epidemics, and genotyping analysis showed 95% homology of the strain with a wild type 1 poliovirus strain isolated in Pakistan in 1995. Neutralization assays with both human sera and monoclonal antibodies were performed to analyze the antigenic structure of the epidemic strain, suggesting its peculiar antigenic characteristics. The presented data underline the current risks of outbreaks due to imported wild poliovirus and emphasize the need to improve vaccination efforts and also the need to implement surveillance in countries free of indigenous wild poliovirus.

scholarly journals The gH-gL Complex of Herpes Simplex Virus (HSV) Stimulates Neutralizing Antibody and Protects Mice against HSV Type 1 Challenge

1998 ◽  
Vol 72 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Tao Peng ◽  
Manuel Ponce-de-Leon ◽  
Hongbin Jiang ◽  
Gary Dubin ◽  
John M. Lubinski ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Antigenic Structure ◽  
Virus Infectivity ◽  
Neutralizing Activity ◽  
Virus Challenge ◽  
Infected Cells ◽  
Simplex Virus ◽  
Hsv 1

The herpes simplex virus type 1 (HSV-1) gH-gL complex which is found in the virion envelope is essential for virus infectivity and is a major antigen for the host immune system. However, little is known about the precise role of gH-gL in virus entry, and attempts to demonstrate the immunologic or vaccine efficacy of gH and gL separately or as the gH-gL complex have not succeeded. We constructed a recombinant mammalian cell line (HL-7) which secretes a soluble gH-gL complex, consisting of gH truncated at amino acid 792 (gHt) and full-length gL. Purified gHt-gL reacted with gH- and gL-specific monoclonal antibodies, including LP11, which indicates that it retains its proper antigenic structure. Soluble forms of gD (gDt) block HSV infection by interacting with specific cellular receptors. Unlike soluble gD, gHt-gL did not block HSV-1 entry into cells, nor did it enhance the blocking capacity of gD. However, polyclonal antibodies to the complex did block entry even when added after virus attachment. In addition, these antibodies exhibited high titers of complement-independent neutralizing activity against HSV-1. These sera also cross-neutralized HSV-2, albeit at low titers, and cross-reacted with gH-2 present in extracts of HSV-2-infected cells. To test the potential for gHt-gL to function as a vaccine, BALB/c mice were immunized with the complex. As controls, other mice were immunized with gD purified from HSV-infected cells or were sham immunized. Sera from the gD- or gHt-gL-immunized mice exhibited high titers of virus neutralizing activity. Using a zosteriform model of infection, we challenged mice with HSV-1. All animals showed some evidence of infection at the site of virus challenge. Mice immunized with either gD or gHt-gL showed reduced primary lesions and exhibited no secondary zosteriform lesions. The sham-immunized control animals exhibited extensive secondary lesions. Furthermore, mice immunized with either gD or gHt-gL survived virus challenge, while many control animals died. These results suggest that gHt-gL is biologically active and may be a candidate for use as a subunit vaccine.

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