THE USE OF BETA-PROPIOLACTONE FOR THE PREPARATION OF VIRUS VACCINES: II. ANTIGENICITY

1957 ◽  
Vol 3 (6) ◽  
pp. 871-877 ◽  
Author(s):  
John R. Polley ◽  
Muriel M. Guerin
Keyword(s):  
Influenza Virus ◽  
Antibody Response ◽  
Specific Antibody ◽  
Guinea Pigs ◽  
Influenza Vaccines ◽  
Live Virus ◽  
Specific Antibody Response

An investigation has been made of the antigenicity of influenza virus suspensions rendered noninfective with beta-propiolactone (BPL). By treatment with BPL under various conditions, influenza vaccines could be prepared which were antigenic as indicated by production of specific antibody response in mice and guinea pigs. These vaccines were also immunizing as evidenced by their capacity to protect mice against challenge with live virus.

scholarly journals Specific antibody response of mice after immunization with COS-7 cell derived avian influenza virus (H5N1) recombinant proteins

2007 ◽  
Vol 5 (1) ◽  
pp. 10 ◽  
Author(s):  
Navin Horthongkham ◽  
Tananun Srihtrakul ◽  
Niracha Athipanyasilp ◽  
Sontana Siritantikorn ◽  
Wannee Kantakamalakul ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Antibody Response ◽  
Avian Influenza Virus ◽  
Recombinant Proteins ◽  
Specific Antibody ◽  
Specific Antibody Response ◽  
Virus H5n1 ◽  
Avian Influenza Virus H5n1

scholarly journals Whole Transcriptome Profiling Identifies CD93 and Other Plasma Cell Survival Factor Genes Associated with Measles-Specific Antibody Response after Vaccination

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0160970 ◽  
Author(s):  
Iana H. Haralambieva ◽  
Michael T. Zimmermann ◽  
Inna G. Ovsyannikova ◽  
Diane E. Grill ◽  
Ann L. Oberg ◽  
...  
Keyword(s):  
Antibody Response ◽  
Cell Survival ◽  
Plasma Cell ◽  
Specific Antibody ◽  
Transcriptome Profiling ◽  
Specific Antibody Response ◽  
Survival Factor ◽  
Whole Transcriptome ◽  
Cell Survival Factor

scholarly journals A highly specific antibody response after protein prime-peptide boost immunization with Eppin/B-cell epitope in mice

2011 ◽  
Vol 7 (8) ◽  
pp. 849-855 ◽  
Author(s):  
Zhengqiong Chen ◽  
Wei He ◽  
Yuzhang Wu ◽  
Ping Yan ◽  
Haiyang He ◽  
...  
Keyword(s):  
Antibody Response ◽  
B Cell ◽  
Specific Antibody ◽  
Cell Epitope ◽  
Specific Antibody Response ◽  
B Cell Epitope ◽  
Boost Immunization

Assessment of the antigen-specific antibody response induced by mucosal administration of a GnRH conjugate entrapped in lipid nanoparticles

2014 ◽  
Vol 10 (5) ◽  
pp. e971-e979 ◽  
Author(s):  
Ayman M. Gebril ◽  
Dimitrios A. Lamprou ◽  
Manal M. Alsaadi ◽  
William H. Stimson ◽  
Alexander B. Mullen ◽  
...  
Keyword(s):  
Antibody Response ◽  
Specific Antibody ◽  
Lipid Nanoparticles ◽  
Specific Antibody Response

Characterization of Donor Specific Antibody Response Following Liver Transplantation in Rats.

2014 ◽  
Vol 98 ◽  
pp. 386-387
Author(s):  
Y. Xie ◽  
Y. Wu ◽  
J. Wang ◽  
J. Ye ◽  
J. Levitsky ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Antibody Response ◽  
Specific Antibody ◽  
Specific Antibody Response

scholarly journals Poor specific antibody response in patients with mucopolysaccaridosis

2019 ◽  
Vol 143 (2) ◽  
pp. AB15
Author(s):  
Marilia M. Moraes ◽  
Isabella B. Manhaes ◽  
Luiza M. Marino ◽  
Julio Cesar Gontijo ◽  
Barbara Luiza B. Cancado ◽  
...  
Keyword(s):  
Antibody Response ◽  
Specific Antibody ◽  
Specific Antibody Response

scholarly journals Anti-factor VIII antibodies of hemophiliac patients are frequently directed towards nonfunctional determinants and do not exhibit isotypic restriction

Blood ◽  
1993 ◽  
Vol 82 (8) ◽  
pp. 2452-2461 ◽  
Author(s):  
JG Gilles ◽  
J Arnout ◽  
J Vermylen ◽  
JM Saint-Remy
Keyword(s):  
Antibody Response ◽  
Factor Viii ◽  
Specific Antibody ◽  
Hemophilia A ◽  
Significant Proportion ◽  
Functional Method ◽  
Specific Antibody Response ◽  
Functional Assays ◽  
Epitope Specificity ◽  
Chromogenic Assay

Abstract A significant proportion of hemophilia A patients receiving transfusions of factor VIII (FVIII) develop a specific antibody response towards FVIII. These antibodies are usually detected by assays in which they inhibit the function of the molecule, such as the Bethesda clotting test. We have prepared anti-FVIII antibodies by specific immunoadsorption from the plasma of four hemophiliacs with stable inhibitor levels. The isotypic distribution of such antibodies was determined and their capacity to bind to insolubilized FVIII was compared with their inhibitory activity in two functional assays, namely, the Bethesda assay and a chromogenic assay. In addition, the FVIII epitope specificity was determined by competition with monoclonal antibodies for the binding to insolubilized FVIII. We show here that (1) anti-FVIII antibodies are not isotypically restricted; thus, a significant proportion of specific IgG2 was found; (2) antibodies are frequently directed towards epitopes of FVIII that are not directly involved in the function of the molecule and therefore escape detection in the Bethesda method or chromogenic assay; and (3) each patient shows a unique pattern of FVIII epitope recognition. We conclude that evaluation of anti-FVIII antibodies by a functional method does not provide an accurate evaluation of the specific antibody response. These findings have important implications for the comparison of the immunogenicity of FVIII molecules produced by different technologies and for the development of methods to control anti-FVIII antibody production.

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