STUDIES ON H. PERTUSSIS LIQUID CULTURES: III. LOCALIZATION OF SURFACE ANTIGENS BY MEANS OF FLUORESCENT ANTIBODY

1956 ◽  
Vol 2 (7) ◽  
pp. 677-683 ◽  
Author(s):  
J. de Repentigny ◽  
A. Frappier
Keyword(s):  
Fluorescent Antibody ◽  
Surface Antigens ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Solid Media ◽  
Antigenic Properties ◽  
Antigenic Material ◽  
Capsular Antigens ◽  
The Relationship

The fluorescent antibody technique was used in an attempt to clarify the relationship between the morphology of the surface of Haemophilus pertussis and the antigenic properties of its capsular antigens. First, specific antibodies were produced by injection into rabbits of agglutinogenic and protective surface washings of the bacilli. Then, these antibodies were made fluorescent and used to mark specifically and in situ the original capsular antigens at the surface of bacilli grown in liquid as well as on solid media. Thus was obtained morphological and specific evidence for the presence of a capsule containing antigenic material.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

scholarly journals IMMUNOFLUORESCENT LOCALIZATION OF ORNITHINE AMINOTRANSFERASE IN RAT LIVER

1970 ◽  
Vol 18 (4) ◽  
pp. 264-267 ◽  
Author(s):  
PATRICIA C. BRENNAN ◽  
C. PERAINO ◽  
R. J. M. FRY ◽  
R. W. SWICK
Keyword(s):  
Fluorescent Antibody ◽  
Protein Diet ◽  
Fluorescent Antibody Technique ◽  
Ornithine Aminotransferase ◽  
Immunofluorescent Localization ◽  
Antibody Technique ◽  
Specific Fluorescence ◽  
Hepatic Cells ◽  
Standard Laboratory Diet

Ornithine aminotransferase (l-ornithine:2-oxoacid aminotransferase) (EC 2.6.1.13) was localized in the livers of rats fed diets differing in protein content with an indirect fluorescent antibody technique. The ornithine aminotransferase specific fluorescence in livers from rats fed a 60% protein diet was distributed throughout the cytoplasm of hepatic cells, and numerous fluorescent cells were observed throughout the lobules. Stained hepatic cells of rats fed a standard laboratory diet (24% protein) appeared to be less numerous and the fluorescence less intense than in rats fed the 60% diet. Livers from rats fed the 0% protein diet showed no specific fluorescence. The identification of ornithine aminotransferase in situ with the fluorescent antibody technique is compatible with measurements of the activity of enzyme extracted from the livers of rats fed similar diets.

Rubella Antibodies in Human Serum: Detection by the Indirect Fluorescent-Antibody Technique

Science ◽  
1964 ◽  
Vol 145 (3635) ◽  
pp. 943-945 ◽  
Author(s):  
G. C. Brown ◽  
H. F. Maassab ◽  
J. A. Veronelli ◽  
T. J. Francis
Keyword(s):  
Human Serum ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Serum Detection

scholarly journals LOCALIZATION OF UROMUCOID IN HUMAN KIDNEY AND IN SECTIONS OF HUMAN KIDNEY STONE WITH THE FLUORESCENT ANTIBODY TECHNIQUE

1965 ◽  
Vol 13 (3) ◽  
pp. 155-160 ◽  
Author(s):  
H. J. KEUTEL
Keyword(s):  
Kidney Stones ◽  
Kidney Stone ◽  
Fluorescent Antibody ◽  
Human Kidney ◽  
Fluorescent Antibody Technique ◽  
Renal Tubules ◽  
Antibody Technique ◽  
The Matrix ◽  
Normal Human ◽  
The Common

Fluorescent labeled antibodies were used for the demonstration of uromucoid. This urine specific mucoprotein is demonstrably present only in the epithelial cells of the proximal segments of the normal human renal tubules and in the matrix of human kidney stones of all the common crystalline compositions.

scholarly journals THE HISTOLOGICAL DISTRIBUTION OF BLOOD GROUP SUBSTANCES A AND B IN MAN

1960 ◽  
Vol 111 (6) ◽  
pp. 785-800 ◽  
Author(s):  
Aron E. Szulman
Keyword(s):  
Blood Group ◽  
Fluorescent Antibody ◽  
Sweat Glands ◽  
Surgical Removal ◽  
Positive Staining ◽  
Fluorescent Antibody Technique ◽  
Blood Group Antigens ◽  
Specific Staining ◽  
Antibody Technique ◽  
Secretor Status

The mapping out of the histologic distribution of blood group antigens A and B in human tissues was performed by means of the fluorescent antibody technique. Human hyperimmune sera were conjugated with fluorescein isocyanate and applied to frozen sections of human material obtained at autopsy or after surgical removal. The material examined encompassed A, B, and AB subjects. In the latter the anti-A and the anti-B conjugate elicited the same picture. Group O tissues were used for controls and were uniformly negative. The secretor status of subjects was determined from the saliva or by the Lewis typing of erythrocytes. The results fall into the following main divisions: Endothelia of Vessels.—Widespread localization was demonstrated in the cell walls of endothelium of capillaries, veins, arteries, and of sinusoidal cells of spleen. Stratified Epithelia.—These showed good outlining of cells of the Malpighian (and the granular, when present) layers. In transitional epithelia, cells of the basal and contiguous layers gave specific staining. Mucus-Secreting Apparatus.—Positive staining was obtained in glands, goblet cells, and secreting surface epithelia. In non-secretors there was no identifiable antigen with the important exception of the deeper parts of gastric foveolae, deeper parts of crypts of Lieberkühn of bowel mucosa and Brunner's glands of the duodenum. Various Organs of Secretion and Excretion.—The pancreas (exocrine portion) and the sweat glands were found to produce the antigen irrespectively of secretor status. Breast, prostate, and endometrial glands on the other hand apparently secrete the antigen in conformity with the subject's secretor:non-secretor make-up. Thus the secretor:non-secretor status governs principally the antigens associated with mucous secretions and this in most but not all locations. The possible nature of this control is briefly discussed.

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