EXTRACTION, PURIFICATION, AND PROPERTIES OF BACILLUS SOTTO TOXIN

1956 ◽  
Vol 2 (4) ◽  
pp. 416-426 ◽  
Author(s):  
T. A. Angus

A proteinaceous toxin has been extracted from sporulated cultures of Bacillus sotto Ishiwata. The toxin is similar in amino acid composition and biological activity to the crystalline inclusions produced by this microorganism and it appears that the toxic protein is associated with the crystals. The toxin is stable, and causes paralysis and death in the larvae of Bombyx mori L. (the silkworm) and other lepidopterous insects. The symptoms caused by the toxin are identical with those caused by ingestion of the whole microorganism.

1975 ◽  
Author(s):  
A.D. Muller ◽  
B. M. Bas ◽  
H. C. Hemker

Staphylocoagulase, an exoprotein of coagulase positive staphylocoagulase, has been purified to a state in which only trace amounts of contaminating proteins are detectable.Purification was more than 35,000 fold, which is 7 times more than the highest value reported in the literature. The yield was about 15%.Aspartic acid was found as a single N-terminal amino acid in this preparation. The molecular weight is 61,000 and the isoelectric point lies at pH 4.53.The amino acid composition was determined.


1969 ◽  
Vol 47 (8) ◽  
pp. 823-825 ◽  
Author(s):  
R. K. O'Dor ◽  
C. O. Parkes ◽  
D. H. Copp

Calcitonin has been extracted from salmon ultimobranchial glands and purified. The amino acid composition indicates similarities to mammalian calcitonin but the salmon hormone has 25 times the biological activity of the most active mammalian material.


2020 ◽  
Vol 28 ◽  
pp. 02001
Author(s):  
Sartika Puspita ◽  
Siti Sunarintyas ◽  
Chairil Anwar ◽  
Ema Mulyawati ◽  
Marsetyawan HNE Soesatyo

A fibroin is a natural polymer that serves as a biomaterial because of the mechanical properties, biocompatible, biodegradable and amino acid composition. The mechanical properties of fibroin are influenced by crystalline structures of amino acid and are related to the amino acid composition. it can accelerate the wound healing because it promotes the proliferation of exposure tissue and inhibits inflammation. The study aimed to identify amino acid of Bombyx mori (B. mori) fibroin cocoon as biomaterial that was cultivated in Central Java Indonesia. Liquid Chromatography/Mass Spectrometry (LC/MS-Water, USA) was employed. This study found that fibroin of B. mori contains seventeen amino acids and the most abundant amino acid is L-Valin 4.81 % (mg kg–1).


1961 ◽  
Vol 15 ◽  
pp. 1790-1791 ◽  
Author(s):  
Erik Jorpes ◽  
Viktor Mutt ◽  
Lars Gunnar Sillén

1977 ◽  
Vol 55 (7) ◽  
pp. 747-754 ◽  
Author(s):  
M. R. Sairam ◽  
Choh Hao Li

Procedures have been described for the isolation of highly purified thyrotropin from frozen or acetone-preserved glands or from side fractions of somatotropin isolation and for the separation of its α and β subunits. The products have been characterized by terminal residue analyses, amino acid composition, carbohydrate content, disc electrophoresis, ultracentrifugation, and biological activity.


1955 ◽  
Vol 77 (14) ◽  
pp. 3908-3913 ◽  
Author(s):  
W. A. Schroeder ◽  
Lois M. Kay ◽  
Barry Lewis ◽  
Nancy Munger

2014 ◽  
Author(s):  
Alexandra Jayne Kermack ◽  
Ying Cheong ◽  
Nick Brook ◽  
Nick Macklon ◽  
Franchesca D Houghton

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