Anaerobic Capacity: A Maximal Anaerobic Running Test Versus the Maximal Accumulated Oxygen Deficit

1996 ◽  
Vol 21 (1) ◽  
pp. 35-47 ◽  
Author(s):  
Neil S. Maxwell ◽  
Myra A. Nimmo
Keyword(s):  
Blood Lactate ◽  
Intermittent Exercise ◽  
Anaerobic Capacity ◽  
Capillary Blood ◽  
Treadmill Running ◽  
Oxygen Deficit ◽  
Blood Samples ◽  
Significant Difference ◽  
Accumulated Oxygen Deficit ◽  
Exercise Treadmill

The present investigation evaluates a maximal anaerobic running test (MART) against the maximal accumulated oxygen deficit (MAOD) for the determination of anaerobic capacity. Essentially, this involved comparing 18 male students performing two randomly assigned supramaximal runs to exhaustion on separate days. Post warm-up and 1, 3, and 6 min postexercise capillary blood samples were taken during both tests for plasma blood lactate (BLa) determination. In the MART only, blood ammonia (BNH3) concentration was measured, while capillary blood samples were additionally taken after every second sprint for BLa determination. Anaerobic capacity, measured as oxygen equivalents in the MART protocol, averaged 112.2 ± 5.2 ml∙kg−1∙min−1. Oxygen deficit, representing the anaerobic capacity in the MAOD test, was an average of 74.6 ± 7.3 ml∙kg−1. There was a significant correlation between the MART and MAOD (r =.83, p <.001). BLa values obtained over time in the two tests showed no significant difference, nor was there any difference in the peak BLa recorded. Peak BNH3 concentration recorded was significantly increased from resting levels at exhaustion during the MART. Key words: supramaximal intermittent exercise, treadmill running performance, blood lactate, ammonia

Effects of Different Methods Used to Take Blood Samples on Blood Glucose Measurements

2021 ◽  
pp. 105477382110247
Author(s):  
Eda Ergin ◽  
Ayten Zaybak
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance Test ◽  
Venous Blood ◽  
Tolerance Test ◽  
Capillary Blood ◽  
Oral Glucose ◽  
Blood Samples ◽  
Significant Difference ◽  
Measurement Results ◽  
Quasi Experimental

The purpose of this study is to compare whether or not there is a difference between venous and capillary blood samples in blood glucose measurements and investigate the effects of different aseptic methods used in skin cleaning before collecting blood samples on measurement results. This quasi-experimental study was conducted with 109 patients. The capillary first and second blood drop values taken from the patients after fasting and at 2 hours following 75 g oral glucose tolerance test (OGTT) and capillary and venous blood glucose values were compared. There was no significant difference between the median venous blood glucose value and the capillary second blood drop value taken after wiping the finger with alcohol. There was no significant difference between the first and second blood drop values of capillary blood glucose 2 hours after OGTT.

scholarly journals Acute administration of high doses of taurine does not substantially improve high-intensity running performance and the effect on maximal accumulated oxygen deficit is unclear

2016 ◽  
Vol 41 (5) ◽  
pp. 498-503 ◽  
Author(s):  
Fabio Milioni ◽  
Elvis de Souza Malta ◽  
Leandro George Spinola do Amaral Rocha ◽  
Camila Angélica Asahi Mesquita ◽  
Ellen Cristini de Freitas ◽  
...  
Keyword(s):  
Oxygen Uptake ◽  
Exercise Intensity ◽  
Maximal Oxygen Uptake ◽  
High Intensity ◽  
Treadmill Running ◽  
Oxygen Deficit ◽  
Time To Exhaustion ◽  
Acute Administration ◽  
Running Performance ◽  
Accumulated Oxygen Deficit

The aim of the present study was to investigate the effects of acute administration of taurine overload on time to exhaustion (TTE) of high-intensity running performance and alternative maximal accumulated oxygen deficit (MAODALT). The study design was a randomized, placebo-controlled, crossover design. Seventeen healthy male volunteers (age: 25 ± 6 years; maximal oxygen uptake: 50.5 ± 7.6 mL·kg−1·min−1) performed an incremental treadmill-running test until voluntary exhaustion to determine maximal oxygen uptake and exercise intensity at maximal oxygen uptake. Subsequently, participants completed randomly 2 bouts of supramaximal treadmill-running at 110% exercise intensity at maximal oxygen uptake until exhaustion (placebo (6 g dextrose) or taurine (6 g) supplementation), separated by 1 week. MAODALT was determined using a single supramaximal effort by summating the contribution of the phosphagen and glycolytic pathways. When comparing the results of the supramaximal trials (i.e., placebo and taurine conditions) no differences were observed for high-intensity running TTE (237.70 ± 66.00 and 277.30 ± 40.64 s; p = 0.44) and MAODALT (55.77 ± 8.22 and 55.06 ± 7.89 mL·kg−1; p = 0.61), which seem to indicate trivial and unclear differences using the magnitude-based inferences approach, respectively. In conclusion, acute 6 g taurine supplementation before exercise did not substantially improve high-intensity running performance and showed an unclear effect on MAODALT.

scholarly journals Analytical performance of lateral flow immunoassay for SARS-CoV-2 exposure screening on venous and capillary blood samples

2020 ◽  
Author(s):  
Margaret A Black ◽  
Guomiao Shen ◽  
Xiaojun Feng ◽  
Wilfredo Garcia Beltran ◽  
Yang Feng ◽  
...  
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Venous Blood ◽  
Capillary Blood ◽  
Lateral Flow ◽  
Antibody Detection ◽  
Lateral Flow Immunoassay ◽  
Blood Samples ◽  
Significant Difference ◽  
Finger Stick

Objectives: Numerous serologic immunoassays have been launched to detect antibodies to SARS-CoV-2, including rapid tests. Here, we validate use of a lateral flow immunoassay (LFI) intended for rapid screening and qualitative detection of anti-SARS-CoV-2 IgM and IgG in serum, plasma, and whole blood, and compare results with ELISA. We also seek to establish the value of LFI testing on blood obtained from a capillary blood sample. Methods: Samples collected by venous blood draw and capillary finger stick were obtained from patients with SARS-CoV-2 detected by RT-qPCR and control patients negative for SARS-CoV-2. Samples were tested with the 2019-nCoV IgG/IgM Detection Kit (Colloidal Gold) lateral flow immunoassay, and antibody calls were compared with results obtained by ELISA. Results: The Biolidics LFI kit shows clinical sensitivity of 92% at 7 days after PCR diagnosis of SARS-CoV-2 on venous blood. Test specificity was 92% for IgM and 100% for IgG. There was no significant difference in detecting IgM and IgG with Biolidics LFI and ELISA at D0 and D7 (p=1.00), except for detection of IgM at D7 (p=0.04). Finger stick whole blood of SARS-CoV-2 patients showed 93% sensitivity for antibody detection. Conclusions: Clinical performance of Biolidics 2019-nCoV IgG/IgM Detection Kit (Colloidal Gold) is comparable to ELISA and showed consistent results across different sample types. Furthermore, we show that capillary blood obtained by finger stick shows similar sensitivity for detecting anti-SARS-CoV-2 IgM and IgG antibodies as venous blood samples. This provides an opportunity for decentralized rapid testing in the community and may allow point-of-care and longitudinal self-testing for the presence of anti-SARS-CoV-2 antibodies.

scholarly journals Validation of a point-of-care capillary lactate measuring device (Lactate Pro 2).

2020 ◽  
Author(s):  
Anette Raa ◽  
Geir Arne Sunde ◽  
Bjørn Bolann ◽  
Reidar Kvåle ◽  
Christopher Bjerkvig ◽  
...  
Keyword(s):  
Intensive Care ◽  
Healthy Volunteers ◽  
Blood Lactate ◽  
Point Of Care ◽  
Reference Method ◽  
Capillary Blood ◽  
Handheld Device ◽  
Intensive Care Patients ◽  
Arterial Blood ◽  
Significant Difference

Abstract Background: The measurement of lactate in emergency medical services has the potential for earlier detection of shock and can be performed with a point-of-care handheld device. Validation of a point-of-care handheld device is required for prehospital implementation. Aim: The primary aim was to validate the accuracy of Lactate Pro 2 in healthy volunteers and in haemodynamically compromised intensive care patients. The secondary aim was to evaluate which sample site, fingertip or earlobe, is most accurate compared to arterial lactate. Methods: Arterial, venous and capillary blood samples from fingertips and earlobes were collected from intensive care patients and healthy volunteers. Arterial and venous blood lactate samples were analysed on a stationary hospital blood gas analyser (ABL800 Flex) as the reference device and compared to the Lactate Pro 2. We used the Bland-Altman method to calculate the limits of agreement and used mixed effect models to compare instruments and sample sites. A total of 49 intensive care patients with elevated lactate and 11 healthy volunteers with elevated lactate were included. Results: There was no significant difference in measured lactate between Lactate Pro 2 and the reference method using arterial blood in either the healthy volunteers or the intensive care patients. Capillary lactate measurement in the fingertip and earlobe of intensive care patients was 47% (95% CI (29% to 68%), p<0.001) and 27% (95% CI (11% to 45%), p<0.001) higher, respectively, than the corresponding arterial blood lactate. In the healthy volunteers, we found that capillary blood lactate in the fingertip was 14% higher than arterial blood lactate (95% CI (4% to 24%), p= 0.003) and no significant difference between capillary blood lactate in the earlobe and arterial blood lactate.Conclusion: Our results showed that the handheld Lactate Pro 2 had good agreement with the reference method using arterial blood in both intensive care patients and healthy volunteers. However, we found that the agreement was poorer using venous blood in both groups. Furthermore, the earlobe may be a better sample site than the fingertip in intensive care patients.

Non-oxidative Energy Supply Correlates with Lactate Transport and Removal in Trained Rowers

2020 ◽  
Vol 41 (13) ◽  
pp. 936-943
Author(s):  
Hugo Maciejewski ◽  
Muriel Bourdin ◽  
Léonard Féasson ◽  
Hervé Dubouchaud ◽  
Laurent André Messonnier
Keyword(s):  
Blood Lactate ◽  
Energy Supply ◽  
Citrate Synthase ◽  
Monocarboxylate Transporter ◽  
Oxygen Deficit ◽  
Muscle Lactate ◽  
Lactate Accumulation ◽  
Accumulated Oxygen Deficit ◽  
Lactate Removal ◽  
Exercise Muscle

AbstractThis study aimed to test if the non-oxidative energy supply (estimated by the accumulated oxygen deficit) is associated with an index of muscle lactate accumulation during exercise, muscle monocarboxylate transporter content and the lactate removal ability during recovery in well-trained rowers. Seventeen rowers completed a 3-min all-out exercise on rowing ergometer to estimate the accumulated oxygen deficit. Blood lactate samples were collected during the subsequent passive recovery to assess individual blood lactate curves, which were fitted to the bi-exponential time function: La(t)= [La](0)+A1·(1–e–γ 1 t)+A2·(1–e–γ 2 t), where the velocity constants γ1 and γ2 (min–1) denote the lactate exchange and removal abilities during recovery, respectively. The accumulated oxygen deficit was correlated with the net amount of lactate released from the previously active muscles (r =0.58, P<0.05), the monocarboxylate transporters MCT1 and MCT4 (r=0.63, P<0.05) and γ2 (r=0.55, P<0.05). γ2 and the lactate release rate at exercise completion were negatively correlated with citrate synthase activity. These findings suggest that the capacity to supply non-oxidative energy during supramaximal rowing exercise is associated with muscle lactate accumulation and transport, as well as lactate removal ability.

Caffeine Improved Time to Exhaustion But Did Not Change Alternative Maximal Accumulated Oxygen Deficit Estimated During a Single Supramaximal Running Bout

2016 ◽  
Vol 26 (6) ◽  
pp. 549-557 ◽  
Author(s):  
Rodrigo De Araujo Bonetti De Poli ◽  
Willian Eiji Miyagi ◽  
Fabio Yuzo Nakamura ◽  
Alessandro Moura Zagatto
Keyword(s):  
Anaerobic Capacity ◽  
Oxygen Deficit ◽  
Time To Exhaustion ◽  
Placebo Condition ◽  
Double Blind ◽  
Caffeine Ingestion ◽  
Total Body Mass ◽  
Accumulated Oxygen Deficit ◽  
Negative Effect ◽  
Positive Effect

The aim of the current study was to investigate the effects of acute caffeine supplementation on anaerobic capacity determined by the alternative maximal accumulated oxygen deficit (MAODALT) in running effort. Eighteen recreational male runners [29 ± 7years; total body mass 72.1 ± 5.8 kg; height 176.0 ± 5.4cm; maximal oxygen uptake (VO2max) 55.8 ± 4.2 ml·kg-1 ·min-1] underwent a graded exercise test. Caffeine (6 mg·kg-1) or a placebo were administered 1 hr before the supramaximal effort at 115% of the intensity associated with VO2max in a double-blind, randomized cross-over study, for MAODALT assessment. The time to exhaustion under caffeine condition (130.2 ± 24.5s) was 11.3% higher (p = .01) than placebo condition (118.8 ± 24.9 s) and the qualitative inference for substantial changes showed a very likely positive effect (93%). The net participation of the oxidative phosphorylation pathway was significantly higher in the caffeine condition (p = .02) and showed a likely positive effect (90%) of 15.3% with caffeine supplementation. The time constant of abrupt decay of excess postexercise oxygen consumption (τ1) was significantly different between caffeine and placebo conditions (p = .03) and showed a likely negative effect (90%), decreasing -8.0% with caffeine supplementation. The oxygen equivalents estimated from the glycolytic and phosphagen metabolic pathways showed a possibly positive effect (68%) and possibly negative effect (78%) in the qualitative inference with caffeine ingestion, respectively. However, the MAODALT did not differ under the caffeine or placebo conditions (p = .68). Therefore, we can conclude that acute caffeine ingestion does not modify the MAODALT, reinforcing the robustness of this method. However, caffeine ingestion can alter the glycolytic and phosphagen metabolic pathway contributions to MAODALT.

Anaerobic capacity and muscle activation during horizontal and uphill running

1997 ◽  
Vol 83 (1) ◽  
pp. 262-269 ◽  
Author(s):  
Mark A. Sloniger ◽  
Kirk J. Cureton ◽  
Barry M. Prior ◽  
Ellen M. Evans
Keyword(s):  
Lower Extremity ◽  
Muscle Activation ◽  
Anaerobic Capacity ◽  
Magnetic Resonance Images ◽  
Muscle Volume ◽  
Treadmill Running ◽  
Oxygen Deficit ◽  
Before And After ◽  
The Mean ◽  
Exercise Induced

Sloniger, Mark A., Kirk J. Cureton, Barry M. Prior, and Ellen M. Evans. Anaerobic capacity and muscle activation during horizontal and uphill running. J. Appl. Physiol. 83(1): 262–269, 1997.—Anaerobic capacity as measured by the maximal or peak oxygen deficit is greater during uphill than during horizontal running. The objective of this study was to determine whether the greater peak oxygen deficit determined during uphill compared with horizontal running is related to greater muscle volume or mass activated in the lower extremity. The peak oxygen deficit in 12 subjects was determined during supramaximal treadmill running at 0 and 10% grade. Exercise-induced contrast shifts in magnetic resonance images were obtained before and after exercise and used to determine the percentage of muscle volume activated. The mean peak oxygen deficit determined for uphill running [2.96 ± 0.63 (SD) liters or 49 ± 6 ml/kg] was significantly greater ( P < 0.05) than for horizontal running (2.45 ± 0.51 liters or 41 ± 7 ml/kg) by 21%. The mean percentage of muscle volume activated for uphill running [73.1 ± 7.4% (SD)] was significantly greater ( P < 0.05) than for horizontal running (67.0 ± 8.3%) by 9%. The differences in peak oxygen deficit (liters) between uphill and horizontal running were significantly related ( y = 8.05 × 10−4 x + 0.35; r = 0.63, SE of estimate = 0.29 liter, P < 0.05) to the differences in the active muscle volume (cm3) in the lower extremity. We conclude that the higher peak oxygen deficit during uphill compared with horizontal running is due in part to increased mass of skeletal muscle activated in the lower extremity.

Assessment of Child-Specific Aerobic Fitness and Anaerobic Capacity by the Use of the Power-Time Relationships Constants

2010 ◽  
Vol 22 (3) ◽  
pp. 454-466 ◽  
Author(s):  
Erwan Leclair ◽  
Benoit Borel ◽  
Delphine Thevenet ◽  
Georges Baquet ◽  
Patrick Mucci ◽  
...  
Keyword(s):  
Critical Power ◽  
Aerobic Power ◽  
Anaerobic Capacity ◽  
Work Capacity ◽  
Constant Load ◽  
Evaluation Methods ◽  
Oxygen Deficit ◽  
Anaerobic Work Capacity ◽  
Accumulated Oxygen Deficit ◽  
Anaerobic Work

This study first aimed to compare critical power (CP) and anaerobic work capacity (AWC), to laboratory standard evaluation methods such as maximal oxygen uptake (V̇O2max) and maximal accumulated oxygen deficit (MAOD). Secondly, this study compared child and adult CP and AWC values. Subjects performed a maximal graded test to determine V̇O2max and maximal aerobic power (MAP); and four constant load exercises. In children, CP (W.kg−1) was related to V̇O2max (ml.kg−1.min−1; r = .68; p = .004). AWC (J.kg−1) in children was related to MAOD (r = .58; p = .018). Children presented lower AWC (J.kg−1; p = .001) than adults, but similar CP (%MAP) values. CP (%MAP and W.kg−1) and AWC (J.kg−1) were significantly related to laboratory standard evaluation methods but low correlation indicated that they cannot be used interchangeably. CP (%MAP) was similar in children and adults, but AWC (J.kg−1) was significantly lower in children. These conclusions support existing knowledge related to child-adults characteristics.

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