Effect of different models of physical exercise on oxidative stress markers in mouse liver

2009 ◽  
Vol 34 (1) ◽  
pp. 60-65 ◽  
Author(s):  
Luciano A. da Silva ◽  
Cleber A. Pinho ◽  
Luis G.C. Rocha ◽  
Talita Tuon ◽  
Paulo C.L. Silveira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Physical Exercise ◽  
Mouse Liver ◽  
Citrate Synthase ◽  
Training Session ◽  
Thiobarbituric Acid ◽  
Oxidative Stress Markers ◽  
Sod Activity ◽  
Stress Markers ◽  
Continuous Running

The aim of this study was to investigate the effect of different protocols of physical exercise on oxidative stress markers in mouse liver. Twenty-eight male CF1 mice (30–35 g) were distributed into 4 groups (n = 7) — untrained (UT), continuous running (CR), downhill running (D-HR), and intermittent running (IR) — and underwent an 8-week training program. Forty-eight hours after the last training session, the animals were killed, and their livers were removed. Blood lactate, creatine kinase, citrate synthase, thiobarbituric acid reactive species, carbonyl, superoxide dismutase (SOD), and catalase (CAT) activities were assayed. Results show a decrease in the level of lipoperoxidation and protein carbonylation in the CR and D-HR groups. SOD activity was significantly increased and CAT activity was reduced in the CR and D-HR groups. Our findings indicate that CR and D-HR may be important for decreasing oxidative damage and in the regulation of antioxidant enzymes (SOD and CAT) in the livers of trained mice.

scholarly journals Redox Homeostasis and Metabolic Profile in Young Female Basketball Players during In-Season Training

Healthcare ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 368
Author(s):  
Rosamaria Militello ◽  
Simone Luti ◽  
Matteo Parri ◽  
Riccardo Marzocchini ◽  
Riccardo Soldaini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Training Session ◽  
Reactive Oxygen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reactive Oxygen Metabolites ◽  
Oxidative Stress Markers ◽  
Basketball Players ◽  
Reactive Oxygen Metabolite ◽  
Stress Markers

Background: Most studies on oxidative stress markers and antioxidant levels have been conducted in male athletes, although female participation in sport has increased rapidly in the past few decades. In particular, it could be important to assess oxidative stress markers in relation to the training load because the anaerobic path becomes predominant in high-intensity actions. Methods: Ten female professional basketball players, performing five 2 h-lasting training sessions per week, and 10 sedentary control women were investigated. Capillary blood and saliva samples were collected in the morning before the training session. The antioxidant capacity and the levels of reactive oxygen metabolites on plasma were determined measuring Reactive Oxygen Metabolite and Biological Antioxidant Potential (d-ROMs and the BAP Test). Salivary cortisol was detected by using commercial enzyme-linked immunosorbent assay kit. Results: The antioxidant capacity (BAP value) was significantly higher in elite basketball players (21.2%; p < 0.05). Conversely, cortisol (51%; p < 0.009) and the levels of oxidative species (d-ROM, 21.9%; p < 0.05) showed a significant decrease in elite athletes.

scholarly journals The subchronic effects of 3,4-methylendioxymethamphetamine on oxidative stress in rat brain

2014 ◽  
Vol 66 (3) ◽  
pp. 1075-1081
Author(s):  
Ivan Simic ◽  
Violeta Iric-Cupic ◽  
Rada Vucic ◽  
Marina Petrovic ◽  
Violeta Mladenovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Rat Brain ◽  
Wistar Rats ◽  
Superoxide Radical ◽  
Reduced Glutathione ◽  
Oxidative Stress Markers ◽  
Sod Activity ◽  
Stress Markers ◽  
Male Wistar Rats

The aim of the present study was to evaluate the subchronic effects of 3,4-methylenedioxymethamphetamine on several oxidative stress markers: index of lipid peroxidation (ILP), superoxide dismutase (SOD) activity, superoxide radical (O2.-) levels, and reduced glutathione (GSH) levels in the frontal cortex, striatum and hippocampus of the rat. The study included 64 male Wistar rats (200-250g). The animals were treated per os with of 5, 10, or 20 mg/kg of 3,4-methylenedioxymethamphetamine (MDMA) every day for 15 days. The subchronic administration of MDMA resulted in an increase in ILP, SOD and O2.-, and a decrease in GSH, from which we conclude that oxidative stress was induced in rat brain.

scholarly journals Effects of Advanced Age, Pituitary Pars Intermedia Dysfunction and Insulin Dysregulation on Serum Antioxidant Markers in Horses

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 444
Author(s):  
Agnieszka Żak ◽  
Natalia Siwińska ◽  
Elżbieta Chełmecka ◽  
Barbara Bażanów ◽  
Ewa Romuk ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sulfhydryl Group ◽  
Study Groups ◽  
Total Serum ◽  
Pars Intermedia ◽  
Oxidative Stress Markers ◽  
Sod Activity ◽  
Stress Markers ◽  
Insulin Dysregulation ◽  
The Impact

The study aims to assess the impact of age, pituitary pars intermedia dysfunction (PPID) and insulin dysregulation (ID) in horses on selected oxidative stress markers. The study includes 32 horses, divided into three groups: “young” adult group (aged 8–16 years old) “geriatric” group (aged 18–24 years old) and the “PPID” group (aged 15–31 years old). The PPID group was further divided into two subgroups: PPID ID+ and PPID ID− based on presence or absence of ID. We measured serum antioxidant stress markers in all horses: total oxidant status (TOS), total antioxidant capacity (TAC), ceruloplasmin (CER), lipofuscin (LPS), malondialdehyde (MDA) and thiols concentrations (containing sulfhydryl group -SH) as well as enzymatic systems: total superoxide dismutase (SOD), cytoplasmic SOD (CuZnSOD), mitochondrial SOD activity (MnSOD). Total serum thiols were significantly lower in the geriatric group and in the PPID group compared to the young group. The MnSOD concentration was higher in the PPID ID+ group compared to the PPID ID−. LPS and MDA concentrations were lower in the PPID ID+ group compared to the PPID ID− group. In the selected study groups of horses, older age, the presence of PPID and ID in the case of PPID had no effect on the studied oxidative stress markers.

scholarly journals Effect of percutaneous transthoracic lung biopsy on oxidative metabolism in sheep

2012 ◽  
Vol 83 (1) ◽  
Author(s):  
Andreza A. Silva ◽  
Danilo O.L. Ferreira ◽  
Bianca P. Santarosa ◽  
Adriano Dias ◽  
Débora C. Damasceno ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Metabolism ◽  
Lung Biopsy ◽  
Thiobarbituric Acid ◽  
Oxidative Stress Markers ◽  
Thiobarbituric Acid Reactive Substances ◽  
Total Glutathione ◽  
Blood Samples ◽  
Stress Markers ◽  
Healthy Sheep

This study aimed to assess the effect of percutaneous transthoracic lung biopsy on the oxidative metabolism of sheep by measuring the oxidative stress markers of superoxide dismutase (SOD), total glutathione (GSH-t), peroxidase (GSH-Px) and thiobarbituric acid reactive substances (TBARS) in the red cells of these animals. Blood samples were collected from 20 clinically healthy sheep prior to, and 30 min after, percutaneous transthoracic lung biopsy. After biopsy, there was a significant decrease (p < 0.05) in SOD and GSH-Px activity, with no significant change (p ≥ 0.05) in GSH-t and TBARS concentrations. These results showed that percutaneous transthoracic lung biopsy did not significantly affect the oxidative metabolism of sheep 30 min after the procedure, which may be used widely in this species without causing serious tissue damage.  

scholarly journals CORRELATION OF SERUM ALBUMIN AND CREATININE WITH OXIDATIVE STRESS MARKERS IN PATIENTS HAVING NEPHROTIC SYNDROME

2021 ◽  
pp. 20-24
Author(s):  
GLORY S. PARMAR ◽  
KINNARI N. MISTRY ◽  
SISHIR GANG
Keyword(s):  
Oxidative Stress ◽  
Nephrotic Syndrome ◽  
Correlation Analysis ◽  
Serum Albumin ◽  
Oxidative Stress Markers ◽  
Indian Children ◽  
Sod Activity ◽  
Stress Markers ◽  
Treatment And Prevention ◽  
The Relationship

Objective: Children with nephrotic syndrome (NS) have a stressful condition, and oxidative damage may impair their treatment response. This study aims to gain a better understanding of the relationship between oxidative stress and NS to lay the basis for further research into improved diagnostic options, treatment, and prevention of the disease. Methods: We took a blood sample from 100 Indian patients aged 2-14 y. Each patient was tested for oxidative stress. The buege method was used to assess MDA levels in patients. The pyrogallol method was used to measure SOD activity in blood serum, and the jollow method was used to measure glutathione levels. Results: The levels of oxidative stress markers (MDA, SOD, and GSH) were compared between NS patients and the control. SOD and GSH concentrations were significantly decreased in the NS group when compared to the control. In contrast, MDA level was significantly higher in the NS group than in the control. In the correlation analysis, we found that the serum SOD activity was significantly positively correlated with serum albumin and creatinine level in patients with NS. Thus, oxidative stress in children with NS is indicated by reduced antioxidant potential because of low albumin. Therefore, it is thought that oxidative stress is implicated in the development of NS in Indian children. Conclusion: We concluded that oxidative stress was intensified in children with NS due to decreased antioxidant levels caused by hypoalbuminemia.

scholarly journals Markers of Oxidative Stress and Antioxidant Status in the Plasma, Urine and Saliva of Healthy Mice

2018 ◽  
pp. 921-934 ◽  
Author(s):  
M. GYURÁSZOVÁ ◽  
A. KOVALČÍKOVÁ ◽  
K. JANŠÁKOVÁ ◽  
K. ŠEBEKOVÁ ◽  
P. CELEC ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thiobarbituric Acid ◽  
Oxidative Status ◽  
Blood Collection ◽  
Reference Ranges ◽  
Oxidative Stress Markers ◽  
Male Adult ◽  
Antioxidant Power ◽  
Stress Markers ◽  
Status Assessment

Oxidative stress markers are usually measured in plasma, a stable environment for biomarkers. Blood collection is invasive, but the use of alternative biofluids is limited, due to high variability. In this study, we aimed to establish reference values for oxidative stress markers in plasma, urine and saliva of adult, healthy mice and to identify some sources of variability. Samples were obtained from 41 female and 37 male adult, healthy mice of the CD-1 strain, aged 95-480 days, weighing 21-55 grams. Reference ranges of TBARS (thiobarbituric acid reactive substances), AOPP (advanced oxidation protein products), fructosamine, GSH/GSSG (reduced and oxidized glutathione) ratio, TAC (total antioxidant capacity), and FRAP (ferric reducing antioxidant power) were measured in plasma and urine, and TBARS, GSH/GSSG ratio, TAC and FRAP in saliva, using standard spectrophotometric and fluorometric methods. Salivary GSH/GSSG and urinary AOPP were higher in females. Urinary fructosamine, GSH/GSSG and FRAP were higher in males. Urinary TAC and FRAP negatively correlated with age, and urinary GSH/GSSG positively correlated with weight. We determined that urine and saliva can be obtained non-invasively from mice, in sufficient amounts for reliable oxidative status assessment. Further studies are needed to uncover whether these biofluids reflect systemic oxidative status in diseases.

scholarly journals Antioxidant Vitamins as Oxidative Stress Markers in Rat Plasma After Physical Exercise - a Short Report

2014 ◽  
Vol 64 (4) ◽  
pp. 277-281 ◽  
Author(s):  
Agata Wawrzyniak ◽  
Jadwiga Hamułka ◽  
Małgorzata Drywień ◽  
Magdalena Górnicka ◽  
Jolanta Pierzynowska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Physical Exercise ◽  
Rat Plasma ◽  
Antioxidant Vitamins ◽  
Short Report ◽  
Oxidative Stress Markers ◽  
Stress Markers

scholarly journals Impact of environmentally relevant concentration of benzophenone-3 on antioxidant enzymes, oxidative stress markers and morphology of gills in Danio rerio (Hamilton)

2021 ◽  
Vol 14 (3) ◽  
pp. 189-196
Author(s):  
S Velanganni ◽  
P Sivakumar ◽  
S Miltonprabu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Danio Rerio ◽  
Thiobarbituric Acid ◽  
Respiratory Physiology ◽  
Control Fish ◽  
Histopathological Analysis ◽  
Oxidative Stress Markers ◽  
Relevant Concentration ◽  
Stress Markers

The current investigation intended to evaluate the effect of Benzophenone-3 (BP-3) at the environmentally relevant concentration (44 μg/L) in the gills of Danio rerio through evaluating oxidative stress markers and histopathological analysis. The adult Zebra fish was exposed to BP-3 at environmentally relevant concentration for 45 days. During the experimental period of 15, 30 and 45 days, lipid peroxidation markers like thiobarbituric acid reactive substances (TBARS) and Hydrogen peroxide (H2O2), antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), Glutathione peroxidase (GPx) and non-enzymatic glutathione (GSH) in the gill and histology of gill were analyzed. The activity of TBARS and H2O2 was found to be significantly higher meanwhile the activities of antioxidant enzymes viz., SOD, CAT, GPx and Glutathione (GSH) level were found to be significantly reduced in the gill of BP-3 treated fish for 30 and 45 days. Additionally, the morphology of gill also showed several abnormal changes in their morphology when compared to control. BP-3 exposure for 15 days elicited only mild alterations in the biochemical and histopathological variables when compared to 30 and 45 days exposure. Further, the values were also non-significant when compared to the control fish. These results demonstrated that the treatment of BP-3 at environmentally relevant concentration could prominently alter the respiratory physiology and metabolism of the gills of Danio rerio.

scholarly journals S-allyl Cysteine, a Garlic Compound, Produces an Antidepressant-Like Effect and Exhibits Antioxidant Properties in Mice

2020 ◽  
Vol 10 (9) ◽  
pp. 592
Author(s):  
Elizabeth Ruiz-Sánchez ◽  
José Pedraza-Chaverri ◽  
Omar N. Medina-Campos ◽  
Perla D. Maldonado ◽  
Patricia Rojas
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Forced Swim Test ◽  
Antioxidant Properties ◽  
Free Radical Scavenger ◽  
Antidepressant Effect ◽  
Radical Scavenger ◽  
Oxidative Stress Markers ◽  
Sod Activity ◽  
Stress Markers

Depression is a psychiatric disorder, and oxidative stress is a significant mechanism of damage in this mood disorder. It is characterized by an enhancement of oxidative stress markers and low concentrations of endogenous antioxidants, or antioxidants enzymes. This suggests that antioxidants could have an antidepressant effect. S-allyl cysteine (SAC) is a compound with antioxidant action or free radical scavenger capacity. The purpose of the current research was to evaluate the antidepressant-like effect as well as the antioxidant role of SAC on a preclinical test, using the Porsolt forced swim test (FST). SAC (30, 70, 120, or 250 mg/kg, ip) was administered to male BALB/c mice daily for 17 days, followed by the FST at day 18. Oxidative stress markers (reactive oxygen species, superoxide production, lipid peroxidation, and antioxidant enzymes activities) were analyzed in the midbrain, prefrontal cortex, and hippocampus. SAC (120 mg/kg) attenuated the immobility scores (44%) in the FST, and protection was unrelated to changes in locomotor activity. This antidepressant-like effect was related to decreased oxidative stress, as indicated by lipid peroxidation and manganese-superoxide dismutase (Mn-SOD) activity in the hippocampus. SAC exerts an antidepressant-like effect that correlated, in part, with preventing oxidative damage in hippocampus.

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