Cytogenetic analysis of Malpighian tubule polytene chromosomes of Culex pipiens (Diptera: Culicidae)

Genome ◽  
1998 ◽  
Vol 41 (6) ◽  
pp. 751-755 ◽  
Author(s):  
Anna Zambetaki ◽  
Nicole Pasteur ◽  
Penelope Mavragani-Tsipidou
Keyword(s):  
Cytogenetic Analysis ◽  
Culex Pipiens ◽  
Polytene Chromosomes ◽  
Malpighian Tubule

Cytogenetic analysis of Malpighian tubule polytene chromosomes of Culex pipiens (Diptera: Culicidae)

Genome ◽  
1998 ◽  
Vol 41 (6) ◽  
pp. 751-755 ◽  
Author(s):  
Anna Zambetaki ◽  
Nicole Pasteur ◽  
Penelope Mavragani-Tsipidou
Keyword(s):  
Culex Pipiens ◽  
Polytene Chromosomes ◽  
Malpighian Tubule ◽  
Malpighian Tubules ◽  
Molecular Cytogenetic ◽  
Routine Basis ◽  
Weak Points ◽  
Chromosome Maps ◽  
Global Vector ◽  
Polytene Nuclei

A simple technique is described for obtaining well-spread and readable Malpighian tubule polytene nuclei of Culex pipiens on a routine basis. Detailed polytene chromosome maps are presented with a description of the most prominent landmarks of each chromosome, the regions with asynapsis and the most frequent weak points identified in the polytene arms. Usable Malpighian tubule polytene chromosomes should facilitate molecular cytogenetic, genetic, and potentially biosystematic studies on this medically important global vector of viral inducing encephalitis.Key words: Culex pipiens, polytene chromosomes, Malpighian tubules, banding pattern, photomap.

Fine Structure of the Malpighian Tubules of the Mosquito, Culex pipiens

1971 ◽  
Vol 29 ◽  
pp. 402-403
Author(s):  
Brendan Clifford
Keyword(s):  
Fine Structure ◽  
Culex Pipiens ◽  
Stellate Cell ◽  
Malpighian Tubule ◽  
Cell Types ◽  
Instar Larva ◽  
Malpighian Tubules ◽  
Calliphora Erythrocephala ◽  
Distinct Cell ◽  
Basal Plasma

An ultrastructural investigation of the Malpighian tubules of the fourth instar larva of Culex pipiens was undertaken as part of a continuing study of the fine structure of transport epithelia.Each of the five Malpighian tubules was found to be morphologically identical and regionally undifferentiated. Two distinct cell types, the primary and stellate, were found intermingled along the length of each tubule. The ultrastructure of the stellate cell was previously described in the Malpighian tubule of the blowfly, Calliphora erythrocephala by Berridge and Oschman.The basal plasma membrane of the primary cell is extremely irregular, giving rise to a complex interconnecting network of basal channels. The compartments of cytoplasm entrapped within this system of basal infoldings contain mitochondria, free ribosomes, and small amounts of rough endoplasmic reticulum. The mitochondria are distinctive in that the cristae run parallel to the long axis of the organelle.

Genetic and cytogenetic analysis of the fruit fly Rhagoletis cerasi (Diptera: Tephritidae)

Genome ◽  
2008 ◽  
Vol 51 (7) ◽  
pp. 479-491 ◽  
Author(s):  
Ilias Kounatidis ◽  
Nikolaos Papadopoulos ◽  
Kostas Bourtzis ◽  
Penelope Mavragani-Tsipidou
Keyword(s):  
Salivary Gland ◽  
Sex Chromosomes ◽  
Cytogenetic Analysis ◽  
Polytene Chromosomes ◽  
Fruit Fly ◽  
Pest Species ◽  
Heteromorphic Sex Chromosomes ◽  
Weak Points ◽  
Heterogametic Sex ◽  
Rhagoletis Cerasi

The European cherry fruit fly, Rhagoletis cerasi , is a major agricultural pest for which biological, genetic, and cytogenetic information is limited. We report here a cytogenetic analysis of 4 natural Greek populations of R. cerasi, all of them infected with the endosymbiotic bacterium Wolbachia pipientis . The mitotic karyotype and detailed photographic maps of the salivary gland polytene chromosomes of this pest species are presented here. The mitotic metaphase complement consists of 6 pairs of chromosomes, including one pair of heteromorphic sex chromosomes, with the male being the heterogametic sex. The analysis of the salivary gland polytene complement has shown a total of 5 long chromosomes (10 polytene arms) that correspond to the 5 autosomes of the mitotic nuclei and a heterochromatic mass corresponding to the sex chromosomes. The most prominent landmarks of each polytene chromosome, the “weak points”, and the unusual asynapsis of homologous pairs of polytene chromosomes at certain regions of the polytene elements are also presented and discussed.

scholarly journals Enzymology, Histological and Ultrastructural Effects of Ar-Turmerone on Culex pipiens pallens Larvae

Insects ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 336
Author(s):  
Jia Liu ◽  
Diana Fernandez ◽  
Yanjin Gao ◽  
Pierre Silvie ◽  
Yongdong Gao ◽  
...  
Keyword(s):  
Culex Pipiens ◽  
Active Sites ◽  
Malpighian Tubule ◽  
Muscle Cells ◽  
Fourth Instar ◽  
Culex Pipiens Pallens ◽  
Detoxifying Enzymes ◽  
Transmission Electron ◽  
Ventral Muscle ◽  
Pipiens Pallens

Our previous article demonstrated that ar-turmerone ((6S)-2-methyl-6-(4-methylphenyl)-2-hepten-4-one) extracted from Curcuma longa L. has a significant larvicidal activity against the fourth instar larvae of Culex pipiens pallens. To reveal the effects of ar-turmerone on C. pipiens pallens larvae, light microscopy and transmission electron microscopy were used to observe the histological and ultrastructure changes in muscle and digestive tissues of fourth instar larvae. It was also revealed by detecting the activity of the acetylcholinesterase (AChE) enzyme and three detoxifying enzymes, including carboxylesterase (CarE), glutathione-S-transferase (GST) and Cytochrome P450 monooxidases (P450). The observation under the light microscope showed that the larvae displayed a disruption of myofibril in ventral muscle cells, the disappearance of nucleolus in the malpighian tubule cells, and the exfoliation of the brush border in midgut epithelial cells, 24 h after treatment. The observation under the transmission electron microscope displayed disorganized Z-lines in the ventral muscle cells, and dissolved membrane of mitochondria, nuclear and endoplasmic reticulum in abdominal cells. The enzymatic activity results showed that ar-turmerone significantly increased the level of detoxifying enzymes, while the activity of AChE was not obviously affected. All the results suggest that the larvicidal mechanism of ar-turmerone is estimated to be stomach poison and the active sites might be the muscle and digestive tissues, and the mode of action of ar-turmerone may be unrelated to AChE.

scholarly journals The Ultrastructure of Mosquitoes : 2. Malpighian tubule of Culex pipiens pallens

1969 ◽  
Vol 20 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Setsuo Suguri ◽  
Yasumasa Tongu ◽  
Kazuo Itano ◽  
Daigoro Sakumoto ◽  
Seiiti Inatomi
Keyword(s):  
Culex Pipiens ◽  
Malpighian Tubule ◽  
Culex Pipiens Pallens ◽  
Pipiens Pallens

Cytogenetic analysis of Malpighian tubule and salivary gland polytene chromosomes of Bactrocera oleae (Dacus oleae) (Diptera: Tephritidae)

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1070-1081 ◽  
Author(s):  
Anna Zambetaki ◽  
Kleanthis Kleanthous ◽  
Penelope Mavragani-Tsipidou
Keyword(s):  
Salivary Gland ◽  
Banding Pattern ◽  
Fat Body ◽  
Polytene Chromosomes ◽  
Malpighian Tubule ◽  
Bactrocera Oleae ◽  
Dacus Oleae ◽  
Somatic Tissues ◽  
Ectopic Pairing ◽  
Tandem Duplications

Photomaps of the Malpighian tubule and the salivary gland polytene chromosomes of Bactrocera oleae (Dacus oleae) are presented and compared with those of the fat body. Five polytene chromosomes (10 polytene arms) corresponding to the five autosomes of the mitotic nuclei, as well as a heterochromatic mass corresponding to the sex chromosomes, are observed in the nuclei of the three somatic tissues. The most prominent features of each polytene chromosome, the reverse tandem duplications, as well as the rather unusual ectopic pairing of the telomeric regions of different chromosome arms, are described. The constancy of the banding pattern based on the analysis of the three larval tissues is discussed.Key words: Bactrocera oleae (Dacus oleae), polytene chromosomes, salivary gland, Malpighian tubule, banding pattern.

scholarly journals Knockdown of the Drosophila GTPase Nucleostemin 1 Impairs Large Ribosomal Subunit Biogenesis, Cell Growth, and Midgut Precursor Cell Maintenance

2009 ◽  
Vol 20 (20) ◽  
pp. 4424-4434 ◽  
Author(s):  
Raphyel Rosby ◽  
Zhengfang Cui ◽  
Emily Rogers ◽  
Megan A. deLivron ◽  
Victoria L. Robinson ◽  
...  
Keyword(s):  
Cell Cycle Progression ◽  
Fluorescent Protein ◽  
Polytene Chromosomes ◽  
Ribosomal Subunit ◽  
Malpighian Tubule ◽  
Ns1 Protein ◽  
Larval Midgut ◽  
Melanotic Tumors ◽  
Green Fluorescent ◽  
Cell Stress Response

Mammalian nucleostemin (NS) is a nucleolar guanosine triphosphate-binding protein implicated in cell cycle progression, stem cell proliferation, and ribosome assembly. Drosophila melanogaster contains a four-member nucleostemin family (NS1–4). NS1 is the closest orthologue to human NS; it shares 33% identity and 67% similarity with human NS. We show that NS1 has intrinsic GTPase and ATPase activity and that it is present within nucleoli of most larval and adult cells. Endogenous NS1 and lightly expressed green fluorescent protein (GFP)-NS1 enrich within the nucleolar granular regions as expected, whereas overexpressed GFP-NS1 localized throughout the nucleolus and nucleoplasm, and to several transcriptionally active interbands of polytene chromosomes. Severe overexpression correlated with the appearance of melanotic tumors and larval/pupal lethality. Depletion of 60% of NS1 transcripts also lead to larval and pupal lethality. NS1 protein depletion>95 correlated with the loss of imaginal island (precursor) cells in the larval midgut and to an apparent block in the nucleolar release of large ribosomal subunits in terminally differentiated larval midgut polyploid cells. Ultrastructural examination of larval Malpighian tubule cells depleted for NS1 showed a loss of cytoplasmic ribosomes and a concomitant appearance of cytoplasmic preautophagosomes and lysosomes. We interpret the appearance of these structures as indicators of cell stress response.

Cytogenetic analysis of mitotic and salivary gland chromosomes in the Medfly Ceratitis capitata

Genome ◽  
1987 ◽  
Vol 29 (1) ◽  
pp. 67-71 ◽  
Author(s):  
A. Zacharopoulou
Keyword(s):  
Salivary Gland ◽  
Key Words ◽  
Cytogenetic Analysis ◽  
Ceratitis Capitata ◽  
Polytene Chromosomes

The present investigation constitutes a first attempt to study the salivary gland chromosomes of Ceratitis capitata. A photographic representation of the polytene chromosomes from the salivary gland of this species is provided and the tips, as well as some important landmarks, are recognized in each arm. There is an XX/XY pair and five pairs of autosomes in the metaphases, but neither the X nor the Y are represented among the banded polytene chromosomes. Key words: Ceratitis capitata, chromosomes (polytene), chromosomes (mitotic), salivary gland chromosomes.

scholarly journals Malpighian tubule polytene chromosomes of Culex quinquefasciatus (Diptera, Culicinae)

2003 ◽  
Vol 98 (3) ◽  
pp. 383-386 ◽  
Author(s):  
Jairo Campos ◽  
Carlos Fernando S Andrade ◽  
Shirlei M Recco-Pimentel
Keyword(s):  
Culex Quinquefasciatus ◽  
Polytene Chromosomes ◽  
Malpighian Tubule

Mitotic and polytene chromosome analysis in Dacus oleae (Diptera: Tephritidae)

Genome ◽  
1992 ◽  
Vol 35 (3) ◽  
pp. 373-378 ◽  
Author(s):  
P. Mavragani-Tsipidou ◽  
G. Karamanlidou ◽  
A. Zacharopoulou ◽  
S. Koliais ◽  
C. Kastritsis
Keyword(s):  
Olive Oil ◽  
Polytene Chromosome ◽  
Genetic Information ◽  
Cytogenetic Analysis ◽  
Fat Body ◽  
Polytene Chromosomes ◽  
Chromosome Analysis ◽  
Olive Tree ◽  
Mitotic Chromosomes ◽  
Dacus Oleae

The present study constitutes the first attempt to construct a photographic map of the polytene chromosomes of Dacus oleae, a pest of the olive tree that causes serious financial damage in all olive oil producing countries. The map was constructed by using the larval fat body cells, the chromosomes of which are representative of the polytene chromosomes of other polytene tissues. In addition, the mitotic chromosomes of brain ganglia were examined, permitting tentative correlations between mitotic and polytene elements. This investigation shows that D. oleae is suitable for cytogenetic analysis in both mitotic and polytene chromosomes, a fact that may prove very useful for obtaining more detailed genetic information on the pest's natural populations.Key words: Dacus oleae, polytene chromosomes, mitotic chromosomes.

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