scholarly journals Rapid and targeted introgression of fgr gene through marker-assisted backcrossing in rice (Oryza sativa L.)

Genome ◽  
2017 ◽  
Vol 60 (12) ◽  
pp. 1045-1050 ◽  
Author(s):  
Acga Cheng ◽  
Ismanizan Ismail ◽  
Mohamad Osman ◽  
Habibuddin Hashim ◽  
Nur Samahah Mohd Zainual
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Cost Effective ◽  
Yield Potential ◽  
Recurrent Parent ◽  
Rice Varieties ◽  
Fragrant Rice ◽  
Self Sufficiency ◽  
Effective Manner ◽  
Fgr Gene

While it is crucial for developing countries like Malaysia to achieve self-sufficiency in rice (Oryza sativa L.), it is equally critical to be able to produce high-quality rice, specifically fragrant rice, which demands are often met through importation. The present study was aimed at developing high-yielding fragrant rice, in a timely and cost-effective manner. A marker-assisted backcross (MABC) approach was optimised to introgress the fragrance gene (fgr) into two high-yielding Malaysian varieties, MR84 and MR219, within two years utilising less than 50 molecular markers. Coupled with phenotypic screening, one single foreground marker (fgr-SNP) and 48 background markers were selected and utilised, revealing recovery of at least 90% of recurrent parent genome (RPG) in merely two backcross generations. Collectively, the yield potential of the developed BC2F2 lines (BLs) was higher (P > 0.05) than the donor parent, MRQ74, and similar (P < 0.05) to both the recurrent parents, MR84 and MR219. In addition, some of the developed BLs showed good grain quality, such as having long grain. We believe that this is the first report comprising the validation and utilisation of the single functional marker system (fgr-SNP) in introgressing the fgr gene into different rice varieties.

scholarly journals Identification of fgr gene and preliminary evaluation for agronomic traits of pandan sticky rice variety (Oryza sativa L.)

2019 ◽  
Vol 02 ◽  
pp. 39-48
Author(s):  
Toan D. Pham
Keyword(s):  
Oryza Sativa ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Rice Production ◽  
Molecular Techniques ◽  
High Yield ◽  
Preliminary Evaluation ◽  
Rice Varieties ◽  
Fgr Gene

Rice (Oryza sativa L.) is the most important food crop in Vietnam particularly in the Mekong Delta. Screening of good quality and high yield rice varieties are needed for rice production in Vietnam. The purpose of this study was to use molecular techniques to identify fgr gene and to evaluate preliminarily agronomic traits of pandan sticky rice variety. The results showed that this variety contained fgr gene. The fragrant allele was amplified by ESP - IFAP primers with a product of 255 bp in size. Similarly, evaluation of agronomic traits showed that the pandan sticky rice variety displayed many desirable characteristics such as plant height of 108 cm, panicle length of 25.6 cm, seed/panicle 135, 100-grain weight 2.07 g, pandan smell, level 2 of alkali digestion, gel consistency 93 mm. These results were useful information and could be applied for improving and providing pandan sticky rice variety for rice production.

scholarly journals Breeding of High Cooking and Eating Quality in Rice by Marker-Assisted Backcrossing (MABc) Using KASP Markers

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 804
Author(s):  
Me-Sun Kim ◽  
Ju-Young Yang ◽  
Ju-Kyung Yu ◽  
Yi Lee ◽  
Yong-Jin Park ◽  
...  
Keyword(s):  
Genetic Background ◽  
Amylose Content ◽  
Yield Potential ◽  
Eating Quality ◽  
Breeding Cycle ◽  
Recurrent Parent ◽  
Rice Varieties ◽  
Breeding Programs ◽  
Genome Wide ◽  
Cooking And Eating Quality

The primary goals of rice breeding programs are grain quality and yield potential improvement. With the high demand for rice varieties of premium cooking and eating quality, we developed low-amylose content breeding lines crossed with Samgwang and Milkyqueen through the marker-assisted backcross (MABc) breeding program. Trait markers of the SSIIIa gene referring to low-amylose content were identified through an SNP mapping activity, and the markers were applied to select favorable lines for a foreground selection. To rapidly recover the genetic background of Samgwang (recurrent parent genome, RPG), 386 genome-wide markers were used to select BC1F1 and BC2F1 individuals. Seven BC2F1 lines with targeted traits were selected, and the genetic background recovery range varied within 97.4–99.1% of RPG. The amylose content of the selected BC2F2 grains ranged from 12.4–16.8%. We demonstrated the MABc using a trait and genome-wide markers, allowing us to efficiently select lines of a target trait and reduce the breeding cycle effectively. In addition, the BC2F2 lines confirmed by molecular markers in this study can be utilized as parental lines for subsequent breeding programs of high-quality rice for cooking and eating.

Genetic Diversity of Local Upland Rice (Oryza sativa L.) Genotypes Based on Agronomic Traits and Yield Potential in North Buton, Indonesia

2017 ◽  
Vol 9 (4) ◽  
pp. 109-117 ◽  
Author(s):  
Budianti Kadidaa ◽  
Gusti Ray Sadimantar ◽  
Suaib . ◽  
La Ode Safuan ◽  
Muhidin .
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Agronomic Traits ◽  
Upland Rice ◽  
Oryza Sativa L ◽  
Yield Potential

Breeding for Improving Human Nutrition and Grain Quality through Biofortification of Traditional Land Races and Improved Cultivars in Rice (Oryza sativa L.)

2017 ◽  
pp. 126-148
Author(s):  
P. Savitha ◽  
R. Usha Kumari
Keyword(s):  
Oryza Sativa ◽  
Tamil Nadu ◽  
Oryza Sativa L ◽  
Farming Systems ◽  
Total Production ◽  
Micronutrient Deficiencies ◽  
Rice Varieties ◽  
The Family ◽  
Iron And Zinc

Rice (Oryza sativa L.) which belongs to the family poaceae, is the life and the prince among cereals as this unique grain helps to sustain two thirds of the world's population. India have traditionally been used or rice cultivation due to availability of water and conductive soil (Nadesa panic). Rice was grown in 155.30 million hectares and around 600 million tonnes of rice harvested each year globally. About 114 countries produce rice with China and India supporting 50 per cent of total production. India stands first in area under cultivation (41.85 mha) and ranks second in production, where as China ranks first in production with just half of the area. India's production is 89.13 million tonnes with a productivity of 2130 kg/ha. Among the rice producing states of India, Tamil Nadu ranks sixth in production (6.53 million tonnes) and second in productivity of 3918 kg/ha with an area of 20.16 lakh ha (Season and Crop Report, 2012). Local farmers in such farming systems retain the traditional varieties based on their knowledge of the quality of the rice varieties and their adaptation to varying agro-ecological situations created by diverse seasons and land situations (Tapasi das & Ashesh Kumar Das, 2014). Among the major micronutrient deficiencies common in rice consuming countries, iron and zinc deficiencies (“hidden hunger”), affect over three billion people worldwide, mostly in developing countries (Welch & Graham, 2004).

Proline and carbohydrate metabolism in rice varieties (Oryza sativa L.) under various drought and recovery conditions

2019 ◽  
Vol 24 (3) ◽  
pp. 376-387 ◽  
Author(s):  
Doan Cong Dien ◽  
Thieu Thi Phong Thu ◽  
Kyi Moe ◽  
Takeo Yamakawa
Keyword(s):  
Oryza Sativa ◽  
Carbohydrate Metabolism ◽  
Oryza Sativa L ◽  
Rice Varieties

scholarly journals Optimization of Malting Conditions for Two Black Rice Varieties, Black Non-Waxy Rice and Black Waxy Rice (Oryza sativa L. Indica)

2011 ◽  
Vol 117 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Ulaiwan Usansa ◽  
Felix Burberg ◽  
Eberhard Geiger ◽  
Werner Back ◽  
Chokchai Wanapu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Black Rice ◽  
Rice Varieties ◽  
Waxy Rice
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