scholarly journals Contrasting morphological and DNA barcode-suggested species boundaries among shallow-water amphipod fauna from the southern European Atlantic coast

Genome ◽  
2017 ◽  
Vol 60 (2) ◽  
pp. 147-157 ◽  
Author(s):  
Jorge Lobo ◽  
Maria S. Ferreira ◽  
Ilisa C. Antunes ◽  
Marcos A.L. Teixeira ◽  
Luisa M.S. Borges ◽  
...  

In this study we compared DNA barcode-suggested species boundaries with morphology-based species identifications in the amphipod fauna of the southern European Atlantic coast. DNA sequences of the cytochrome c oxidase subunit I barcode region (COI-5P) were generated for 43 morphospecies (178 specimens) collected along the Portuguese coast which, together with publicly available COI-5P sequences, produced a final dataset comprising 68 morphospecies and 295 sequences. Seventy-five BINs (Barcode Index Numbers) were assigned to these morphospecies, of which 48 were concordant (i.e., 1 BIN = 1 species), 8 were taxonomically discordant, and 19 were singletons. Twelve species had matching sequences (<2% distance) with conspecifics from distant locations (e.g., North Sea). Seven morphospecies were assigned to multiple, and highly divergent, BINs, including specimens of Corophium multisetosum (18% divergence) and Dexamine spiniventris (16% divergence), which originated from sampling locations on the west coast of Portugal (only about 36 and 250 km apart, respectively). We also found deep divergence (4%–22%) among specimens of seven species from Portugal compared to those from the North Sea and Italy. The detection of evolutionarily meaningful divergence among populations of several amphipod species from southern Europe reinforces the need for a comprehensive re-assessment of the diversity of this faunal group.

2018 ◽  
Vol 61 (4) ◽  
pp. 365-371 ◽  
Author(s):  
Jessica Schiller ◽  
Dagmar Lackschewitz ◽  
Christian Buschbaum ◽  
Karsten Reise ◽  
Shaojun Pang ◽  
...  

Abstract The kelp Undaria pinnatifida, native to East Asian shores, was unintentionally introduced with Pacific oysters into the Mediterranean in 1971. Intentional introduction from there to the French Atlantic coast 12 years later led to a gradual spread to the British Isles and the North Sea. Here, we report on the northernmost established population in continental Europe, and suggest a further spread into Scandinavian waters to be almost inevitable. In 2016, several thalli were found washed ashore at the eastern side of the island of Sylt in the northern Wadden Sea (German Bight, Eastern North Sea). Most specimens bore fertile sporophylls and thallus lengths of >1 m were common. In June 2017, 91 sporophytes were found attached to a mixed bed of Pacific oysters and native blue mussels, located just below low tide level in a moderately sheltered position. Mean thallus length was 0.2 m and the longest 0.7 m. Most had distinctive sporophylls and released spores in the laboratory. From sporophylls collected in the previous year, we successfully reared a new generation, demonstrating the kelp’s potential for further spread by natural means or human vectors.


2021 ◽  
Vol 4 ◽  
Author(s):  
Magdalini Christodoulou ◽  
Berry van der Hoorn ◽  
Laure Van den Bulcke ◽  
Sofie Derycke ◽  
Annelies De Backer ◽  
...  

Benthic macroinvertebrates are key components in environmental impact assessments. Nevertheless, their use as bioindicators can be constrained by the time- and cost-consuming processes needed for their morphological identification. The recent advances in high-throughput sequencing, particularly DNA metabarcoding can provide an alternative to morphology-based approaches. The main limitation for DNA-based tools to be implemented in biomonitoring projects is the considerable investment needed to build high-quality and curated taxonomic reference DNA sequence libraries for species identification. To begin addressing this shortage, Interreg NSR project GEANS aim in developing a curated DNA reference library based on mitochondrial cytochrome c oxidase subunit I (COI) for the North Sea macrobenthos that will serve as the backbone of all the molecular protocols. For the collection of the macroinvertebrates, a targeted sampling campaign is underway across the North Sea Region, while existing collections are being scanned. Macrobenthic specimens are being processed following a series of standardised work flows covering sampling, morphological identifications, molecular lab processing and data handling. GEANS' reference library, currently holds DNA barcodes for 3443 specimens collected from various areas of the North Sea. In total 11 phyla, 28 classes, 80 orders, 280 families, 428 genera and 586 species (37 non-indigenous species) are represented within the library. Species-level identification is taxonomically verified for over 90 % of the species, while genus-level identifications are verified for almost 100 % of the genera. GEANS reference library covers so far over 30 % of North Sea species and its constantly enriched. All specimens are photographed and together with the accompanying sequences, collection and taxonomic data are archived in a dedicate BOLD project. Voucher specimens and DNA extracts are archived at three different institutes in Germany (Senckenberg am Meer), the Netherlands (Naturalis) and Belgium (ILVO). Once available, this DNA-library will be based on taxonomically well-curated specimens and it will support the implementation of fast, cost-efficient and reliable DNA-based identifications and subsequently environmental health assessments in the North Sea.


2001 ◽  
Vol 80 (1) ◽  
pp. 33-52 ◽  
Author(s):  
K.W. Glennie

AbstractOnce the great size of the Groningen Field was fully realized late in 1963, exploration in the southern North Sea was a natural development as the reservoir bedding dipped westward. The origin of that bedding was not certain, one possibility, dune sands, led immediately to a program of desert studies.Licensing regulations for Netherlands waters were not finalized until 1967, offshore exploration beginning with the award of First Round licenses in March 1968. In the UK area, the Continental Shelf Act came into force in May 1964, paving the way for offshore seismic, the first well being spudded late in that year. The first two wells were drilled on the large Mid North Sea High; both were dry, the targeted Rotliegend sandstones being absent. Then followed a series of Rotliegend gas discoveries, large and small, west of Groningen, so that by the time exploration began in Netherlands waters the UK monopoly market was saturated and exploration companies were already looking north for other targets including possible oil.The Rotliegend was targeted in the earliest wells of the UK central North Sea even though there had already been a series of intriguing oil shows in Chalk and Paleocene reservoirs in Danish and Norwegian waters. These were followed early in 1968 by the discovery of gas in Paleocene turbidites at Cod, near the UK-Norway median line. The first major discovery was Ekofisk in 1969, a billion-barrel Maastrichtian to Danian Chalk field. Forties (1970) confirmed the potential of the Paleocene sands as another billion barrel find, while the small Auk Field extended the oil-bearing stratigraphy down to the Permian. In 1971, discovery of the billion-barrel Brent field in a rotated fault block started a virtual ‘stampede’ to prove-up acreage awarded in the UK Fourth Round (1972) before the 50% statutory relinquishment became effective in 1978.Although the geology of much of the North Sea was reasonably well known by the end of the 1970s, new oil and gas reservoirs continued to be discovered during the next two decades. Exploration proved the Atlantic coast of Norway to be a gas and gas-condensate area. The stratigraphiC range of reservoirs extended down to the Carboniferous (gas) and Devonian (oil), while in the past decade, forays into the UK Atlantic Margin and offshore Ireland met with mixed success. During this hectic activity, Netherlands exploration confirmed a range of hydrocarbon-bearing reservoirs; Jurassic oil in the southern Central Graben, Jurassic-Cretaceous oil derived from a Liassic source mainly onshore and, of course, more gas from the Rotliegend. German exploration had mixed fortunes, with no commercial gas in the North Sea and high nitrogen content in Rotliegend gas in the east. Similarly in Poland, where several small Zechstein oil fields were discovered, the Rotliegend gas was nitrogen rich. The discovery of some 100 billion barrels of oil and oil equivalent beneath the waters of the North Sea since 1964 led to an enormous increase in geological knowledge, making it probably the best known area of comparable size in the World. The area had a varied history over the past 500 million years: platete-tonic movement, faulting, igneous activity, climatic change, and deposition in a variety of continental and marine environments, leading to complex geometrical relationships between source rock, reservoir and seal, and to the reasons for diagenetic changes in the quality of the reservoir sequences. Led by increasingly sophisticated seismic, drilling and wireline logging, and coupled with academic research, the North Sea developed into a giant geological laboratory where ideas were tested and extended industry-wide.


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