Construction of a genetic linkage map and QTL analysis in bambara groundnut

Genome ◽  
2016 ◽  
Vol 59 (7) ◽  
pp. 459-472 ◽  
Author(s):  
Nariman Salih Ahmad ◽  
Endah Sri Redjeki ◽  
Wai Kuan Ho ◽  
Siise Aliyu ◽  
Katie Mayes ◽  
...  

Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous underutilized legume that has the potential to improve food security in semi-arid Africa. So far, there are a lack of reports of controlled breeding populations that could be used for variety development and genetic studies. We report here the construction of the first genetic linkage map of bambara groundnut using a F3 population derived from a “narrow” cross between two domesticated landraces (Tiga Nicuru and DipC) with marked divergence in phenotypic traits. The map consists of 238 DArT array and SSR based markers in 21 linkage groups with a total genetic distance of 608.3 cM. In addition, phenotypic traits were evaluated for a quantitative trait loci (QTL) analysis over two generations. A total of 36 significant QTLs were detected for 19 traits. The phenotypic effect explained by a single QTL ranged from 11.6% to 49.9%. Two stable QTLs were mapped for internode length and growth habit. The identified QTLs could be useful for marker-assisted selection in bambara groundnut breeding programmes.

2018 ◽  
pp. 25-30
Author(s):  
D. Torello Marinoni ◽  
N. Valentini ◽  
E. Portis ◽  
A. Acquadro ◽  
C. Beltramo ◽  
...  

2019 ◽  
Vol 99 (5) ◽  
pp. 599-610
Author(s):  
Junhuan Zhang ◽  
Haoyuan Sun ◽  
Li Yang ◽  
Fengchao Jiang ◽  
Meiling Zhang ◽  
...  

A high-density genetic map of apricot (Prunus armeniaca L.) was constructed using an F1 population constructed by crossing two main Chinese cultivars ‘Chuanzhihong’ and ‘Luotuohuang’, coupled with a recently developed reduced representation library (RRL) sequencing. The average sequencing depth was 38.97 in ‘Chuanzhihong’ (female parent), 33.05 in ‘Luotuohuang’ (male parent), and 8.91 in each progeny. Based on the sequencing data, 12 451 polymorphic markers were developed and used in the construction of the genetic linkage map. The final map of apricot comprised eight linkage groups, including 1991 markers, and covered 886.25 cM of the total map length. The average distance between adjacent markers was narrowed to 0.46 cM. Gaps larger than 5 cM only accounted for <0.33%. To our knowledge, this map is the densest genetic linkage map that is currently available for apricot research. It is a valuable linkage map for quantitative trait loci (QTLs) identification of important agronomic traits. Moreover, the high marker density and well-ordered markers that this linkage map provides will be useful for molecular breeding of apricot as well. In this study, we applied this map in the QTL analysis of an important agronomic trait, pistil abortion. Several QTLs were detected and mapped respectively to the middle regions of LG5 (51.005∼59.4 cM) and LG6 (72.884∼76.562 cM), with nine SLAF markers closely linked to pistil abortion. The high-density genetic map and QTLs detected in this study will facilitate marker-assisted breeding and apricot genomic study.


2017 ◽  
Vol 49 (3) ◽  
pp. 1293-1301 ◽  
Author(s):  
Tsubasa Uchino ◽  
Erina Hosoda ◽  
Yoji Nakamura ◽  
Motoshige Yasuike ◽  
Miyuki Mekuchi ◽  
...  

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