Molecular mapping of five soybean genes involved in male-sterility, female-sterility

Genome ◽  
2015 ◽  
Vol 58 (4) ◽  
pp. 143-149 ◽  
Author(s):  
Benjamin Speth ◽  
Joshua P. Rogers ◽  
Napatsakorn Boonyoo ◽  
A.J. VanMeter ◽  
Jordan Baumbach ◽  
...  
Keyword(s):  
Molecular Mapping ◽  
Female Sterility ◽  
Chromosome 11 ◽  
Male Sterile ◽  
Sterile Female ◽  
Fertility Reduction ◽  
And Function ◽  
Fertility Genes ◽  
Sterility Genes

In soybean, asynaptic and desynaptic mutants lead to abnormal meiosis and fertility reduction. Several male-sterile, female-sterile mutants have been identified and studied in soybean, however, some of these mutants have not been mapped to locations on soybean chromosomes. The objectives of this study were to molecularly map five male-sterile, female-sterile genes (st2, st4, st5, st6, and st7) in soybean and compare the map locations of these genes with already mapped sterility genes. Microsatellite markers were used in bulked segregant analyses to locate all five male-sterile, female-sterile genes to soybean chromosomes, and markers from the corresponding chromosomes were used on F2 populations to generate genetic linkage maps. The st2, st4, st5, st6, and st7 genes were located on molecular linkage group (MLG) B1 (chromosome 11), MLG D1a (chromosome 01), MLG F (chromosome 13), MLG B2 (chromosome 14), and D1b (chromosome 02), respectively. The st2, st4, st5, st6, and st7 genes were flanked to 10.3 (∼399 kb), 6.3 (∼164 kb), 3.9 (∼11.8 Mb), 11.0 (∼409 kb), and 5.3 cM (∼224 kb), and the flanked regions contained 57, 17, 362, 52, and 17 predicted genes, respectively. Future characterization of candidate genes should facilitate identification of the male- and female-fertility genes, which may provide vital insights on structure and function of genes involved in the reproductive pathway in soybean.

scholarly journals FERTILITY GENES IN NATURAL POPULATIONS OF DROSOPHILA MELANOGASTER. I. FREQUENCY, ALLELISM AND PERSISTENCE OF STERILITY GENES

Genetics ◽  
1973 ◽  
Vol 74 (2) ◽  
pp. 351-361
Author(s):  
Chozo Oshima ◽  
Takao K Watanabe
Keyword(s):  
Natural Populations ◽  
Pleiotropic Effect ◽  
Female Sterility ◽  
Male And Female ◽  
Random Combination ◽  
Cage Population ◽  
Male And Female Sterility ◽  
Fertility Genes ◽  
Sterility Genes ◽  
Lethal Genes

ABSTRACT Three or four percent of the wild flies in natural populations of D. melanogaster have been found to be sterile. An analysis of sterility associated with the second chromosome revealed a much lower frequency of genetically sterile flies. The accumulation of sterility genes in a cage population was proportional to that of lethal genes, as were their equilibrium frequencies in several natural populations. Many sterile chromosomes were associated with low viability due to pleiotropic effects. The number of chromosomes leading to sterility in both sexes was larger than the expectation based on random combination of male and female sterility genes. This suggests that there is some linkage disequilibrium between male and female sterility genes, as well as a pleiotropic effect of single sterility genes. Some sterility genes were maintained in natural and cage populations, and the patterns of persistence of the sterility genes were very similar to those of lethal genes.

scholarly journals Microscopic Characterization of a Transposon-Induced Male-Sterile, Female-Sterile Mutant in Glycine max L.

2017 ◽  
Vol 178 (8) ◽  
pp. 629-638
Author(s):  
Katherine A. Thilges ◽  
Mark A. Chamberlin ◽  
Marc C. Albertsen ◽  
Harry T. Horner
Keyword(s):  
Glycine Max ◽  
Male Sterile ◽  
Sterile Female ◽  
Glycine Max L ◽  
Sterile Mutant

Molecular mapping of three male-sterile, female-fertile mutants and generation of a comprehensive map of all known male sterility genes in soybean

Genome ◽  
2014 ◽  
Vol 57 (3) ◽  
pp. 155-160 ◽  
Author(s):  
Yang Yang ◽  
Benjamin D. Speth ◽  
Napatsakorn Boonyoo ◽  
Eric Baumert ◽  
Taylor R. Atkinson ◽  
...  
Keyword(s):  
Linkage Group ◽  
Male Sterility ◽  
Genetic Linkage ◽  
Chromosome 2 ◽  
Linkage Maps ◽  
Male Sterile ◽  
Molecular Linkage Group ◽  
Sterile Female ◽  
Genetic Linkage Maps ◽  
Sterility Genes

In soybean, an environmentally stable male sterility system is vital for making hybrid seed production commercially viable. Eleven male-sterile, female-fertile mutants (ms1, ms2, ms3, ms4, ms5, ms6, ms7, ms8, ms9, msMOS, and msp) have been identified in soybean. Of these, eight (ms2, ms3, ms5, ms7, ms8, ms9, msMOS, and msp) have been mapped to soybean chromosomes. The objectives of this study were to (i) locate the ms1, ms4, and ms6 genes to soybean chromosomes; (ii) generate genetic linkage maps of the regions containing these genes; and (iii) develop a comprehensive map of all known male-sterile, female-fertile genes in soybean. The bulked segregant analysis technique was used to locate genes to soybean chromosomes. Microsatellite markers from the corresponding chromosomes were used on F2 populations to generate genetic linkage maps. The ms1 and ms6 genes were located on chromosome 13 (molecular linkage group F) and ms4 was present on chromosome 2 (molecular linkage group D1b). Molecular analyses revealed markers Satt516, BARCSOYSSR_02_1539, and AW186493 were located closest to ms1, ms4, and ms6, respectively. The ms1 and ms6 genes, although present on the same chromosome, were independently assorting with a genetic distance of 73.7 cM. Using information from this study and compiled information from previously published male sterility genes in soybean, a comprehensive genetic linkage map was generated. Eleven male sterility genes were present on seven soybean chromosomes. Four genes were present in two regions on chromosome 2 (molecular linkage group D1b) and two genes were present on chromosome 13 (molecular linkage group F).

Influence of the soybean male-sterile gene (ms1) on the development of the female gametophyte

1985 ◽  
Vol 27 (2) ◽  
pp. 200-209 ◽  
Author(s):  
John C. Kennell ◽  
Harry T. Horner
Keyword(s):  
Glycine Max ◽  
Female Gametophyte ◽  
Field Data ◽  
Subsequent Development ◽  
Female Sterility ◽  
Male Sterile ◽  
Sterile Female ◽  
Glycine Max L

Megasporogenesis and megagametogenesis were examined in a male-sterile, female-fertile mutant (ms1) of soybean (Glycine max (L.) Merr.). Multinucleate (more than eight) megagametophytes resulted from failure of postmeiotic cytokinesis that leads to a coenomegaspore of four haploid nuclei. Fusion of some nuclei may occur. Subsequent development results in mature megagametophytes with up to four eggs, or often abortion. These results support field data that show the male-sterile ms1 gene is associated with increased frequencies of polyembryony, polyploidy, haploidy, and reduced female fertility.Key words: Glycine max, female sterility, polyembryony, polyploidy, megasporogenesis, megagametogenesis.

Molecular mapping of 36 soybean male-sterile, female-sterile mutants

2008 ◽  
Vol 117 (5) ◽  
pp. 711-719 ◽  
Author(s):  
R. G. Palmer ◽  
D. Sandhu ◽  
K. Curran ◽  
M. K. Bhattacharyya
Keyword(s):  
Molecular Mapping ◽  
Male Sterile ◽  
Sterile Female

Characterization and mapping of a male-sterility mutant, tapetum desquamation (t), in rice

Genome ◽  
2008 ◽  
Vol 51 (5) ◽  
pp. 368-374 ◽  
Author(s):  
Yi Zhang ◽  
Jin-Xiong Mao ◽  
Kun Yang ◽  
Yun-Feng Li ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Male Sterility ◽  
Recessive Gene ◽  
Physical Distance ◽  
Anther Wall ◽  
Isogenic Line ◽  
Male Sterile ◽  
The Status ◽  
Microspore Stage ◽  
And Function

A spontaneously mutated male-sterile material was found among the offspring of the indica restorer line Jinhuiyihao. To understand the status and function of the related gene and clone the gene, a near-isogenic line (NIL) of the male sterility was bred, and characterization of the mutant and gene mapping were performed. The results indicated that there are obvious differences between the male-sterile NIL and the indica maintainer line II-32B. The anther size of the NIL is smaller than that of II-32B, and the anther color is white in the NIL but yellow in II-32B. No pollen from the matured anther in the NIL was observed to be stained using KI–I2 solution. In transverse sections of the sterile anther, at early microspore stage the cytoplasm of the tapetum concentrates but the tapetum itself does not degenerate after microspores are released from the tetrads; the tapetum then desquamates from the anther wall and enwraps microspores; subsequently, the surrounded microspores collapse completely at late microspore and early bicellular pollen stages. Inheritance analysis showed that the male sterility was controlled by a single recessive gene, ostd (t). This gene was mapped between the SSR markers RM7434 and RM275 on chromosome 6, and the physical distance from RM7434 to RM275 is about 389 kb.

Functional characterization of human brown adipose tissue metabolism

2020 ◽  
Vol 477 (7) ◽  
pp. 1261-1286 ◽  
Author(s):  
Marie Anne Richard ◽  
Hannah Pallubinsky ◽  
Denis P. Blondin
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Functional Characterization ◽  
Human Infants ◽  
Positron Emission ◽  
Brown Adipose ◽  
Treatment Of Obesity ◽  
And Function

Brown adipose tissue (BAT) has long been described according to its histological features as a multilocular, lipid-containing tissue, light brown in color, that is also responsive to the cold and found especially in hibernating mammals and human infants. Its presence in both hibernators and human infants, combined with its function as a heat-generating organ, raised many questions about its role in humans. Early characterizations of the tissue in humans focused on its progressive atrophy with age and its apparent importance for cold-exposed workers. However, the use of positron emission tomography (PET) with the glucose tracer [18F]fluorodeoxyglucose ([18F]FDG) made it possible to begin characterizing the possible function of BAT in adult humans, and whether it could play a role in the prevention or treatment of obesity and type 2 diabetes (T2D). This review focuses on the in vivo functional characterization of human BAT, the methodological approaches applied to examine these features and addresses critical gaps that remain in moving the field forward. Specifically, we describe the anatomical and biomolecular features of human BAT, the modalities and applications of non-invasive tools such as PET and magnetic resonance imaging coupled with spectroscopy (MRI/MRS) to study BAT morphology and function in vivo, and finally describe the functional characteristics of human BAT that have only been possible through the development and application of such tools.

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