Genetic control of immunity to Turnip mosaic virus (TuMV) pathotype 1 in Brassica rapa (Chinese cabbage)

Genome ◽  
2014 ◽  
Vol 57 (8) ◽  
pp. 419-425 ◽  
Author(s):  
Derek J. Lydiate ◽  
Rachel L. Rusholme Pilcher ◽  
Erin E. Higgins ◽  
John A. Walsh
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Turnip Mosaic Virus ◽  
Rflp Markers ◽  
Resistance Locus ◽  
Chromosome 6 ◽  
A Genome ◽  
Virus Interactions

Turnip mosaic virus (TuMV) is the major virus infecting crops of the genus Brassica worldwide. A dominant resistance gene, TuRB01b, that confers immunity to the virus isolate UK 1 (a representative pathotype 1 isolate of TuMV) on Brassica rapa was identified in the Chinese cabbage cultivar Tropical Delight. The TuRB01b locus was mapped to a 2.9-cM interval on B. rapa chromosome 6 (A6) that was flanked by RFLP markers pN101e1 and pW137e1. This mapping used a first backcross (B1) population segregating for the resistance gene at TuRB01b and sets of RFLP markers employed in previous mapping experiments in Brassica. Virus–plant interaction phenotypes were assayed in inbred progeny derived from B1 individuals to allow different virus isolates to be tested. Comparative mapping confirmed that A6 of B. rapa was equivalent to chromosome 6 of Brassica napus (A6) and that the map position of TuRB01b in B. rapa could be identical to that of TuRB01 in B. napus. Detailed evaluation of plant–virus interactions showed that TuRB01 and TuRB01b had indistinguishable specificities to a range of TuMV isolates. The possibility that TuRB01 and TuRB01b represent similar or identical alleles at the same A genome resistance locus suggests that B. napus acquired TuRB01 from the B. rapa gene pool.

Identification and mapping of a novel dominant resistance gene, TuRB07 to Turnip mosaic virus in Brassica rapa

2013 ◽  
Vol 127 (2) ◽  
pp. 509-519 ◽  
Author(s):  
Mina Jin ◽  
Soo-Seong Lee ◽  
Lin Ke ◽  
Jung Sun Kim ◽  
Mi-Suk Seo ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Brassica Rapa ◽  
Turnip Mosaic Virus

Inheritance and development of EST-SSR marker associated with turnip mosaic virus resistance in Chinese cabbage

2011 ◽  
Vol 91 (4) ◽  
pp. 707-715 ◽  
Author(s):  
Qiaoyun Li ◽  
Haishen Tong ◽  
Zhigang Zhang ◽  
Zhizhong Zhao ◽  
Xiyun Song
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Chinese Cabbage ◽  
Virus Resistance ◽  
Ssr Marker ◽  
Recessive Gene ◽  
Turnip Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
The Cross ◽  
Tumv Resistance

Li, Q., Tong, H., Zhang, Z., Zhao, Z. and Song, X. 2011. Inheritance and development of EST-SSR marker associated with turnip mosaic virus resistance in Chinese cabbage. Can. J. Plant Sci. 91: 707–715. Turnip mosaic virus (TuMV) is one of the major pathogens infecting Brassica crops (including Chinese cabbage), and often causes serious reductions in yield and quality. Breeding for resistant cultivars is complicated by the existence of numerous TuMV strains and isolates. The objective of this research was to determine the mode of inheritance and to develop molecular markers associated with TuMV resistance in Chinese cabbage. F1 and F2 populations were developed from the cross between 71-36-2 (susceptible) and 73 (resistant) lines and mechanically inoculated with TuMV-C4. Inheritance analysis by visual scoring and enzyme-linked immunosorbent assay (ELISA) indicated that resistance to TuMV-C4 in this cross was controlled by one recessive gene. A total of 132 EST-SSR primers were designed from EST sequences available in public databases. Seven primers detected polymorphism between parental genotypes. Marker HCC259 was associated with the TuMV resistance Ph-retr02. The distance between the marker and the TuMV resistance gene retr02 was 3.8 cM. This is the first co-dominant marker linked to the TuMV resistance gene with a distance less than 5.0 cM in Chinese cabbage. This marker was suitable for TuMV-C4 resistance screening in progenies from the cross between lines 73 (resistant) and susceptible line, 71-36-2. The usefulness of this marker was validated in 21 additional resistant and susceptible lines. This marker has the potential to simplify and accelerate breeding Chinese cabbage cultivars resistant to TuMV-C4.

Genetic mapping of the novel Turnip mosaic virus resistance gene TuRB03 in Brassica napus

2003 ◽  
Vol 107 (7) ◽  
pp. 1169-1173 ◽  
Author(s):  
S. L. Hughes ◽  
P. J. Hunter ◽  
A. G. Sharpe ◽  
M. J. Kearsey ◽  
D. J. Lydiate ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Mapping ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Turnip Mosaic Virus ◽  
The Novel ◽  
Virus Resistance Gene

scholarly journals High-resolution mapping of Rym14Hb, a wild relative resistance gene to barley yellow mosaic disease

2020 ◽  
Author(s):  
Hélène Pidon ◽  
Neele Wendler ◽  
Antje Habekuβ ◽  
Anja Maasberg ◽  
Brigitte Ruge-Wehling ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Resistance Mechanism ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Linkage Drag ◽  
Resistance Locus ◽  
Wild Relative ◽  
Yellow Mosaic Disease ◽  
Barley Breeding

Abstract Key message We mapped the Rym14Hb resistance locus to barley yellow mosaic disease in a 2Mbp interval. The co-segregating markers will be instrumental for marker-assisted selection in barley breeding. Abstract Barley yellow mosaic disease is caused by Barley yellow mosaic virus and Barley mild mosaic virus and leads to severe yield losses in barley (Hordeum vulgare) in Central Europe and East-Asia. Several resistance loci are used in barley breeding. However, cases of resistance-breaking viral strains are known, raising concerns about the durability of those genes. Rym14Hb is a dominant major resistance gene on chromosome 6HS, originating from barley’s secondary genepool wild relative Hordeum bulbosum. As such, the resistance mechanism may represent a case of non-host resistance, which could enhance its durability. A susceptible barley variety and a resistant H. bulbosum introgression line were crossed to produce a large F2 mapping population (n = 7500), to compensate for a ten-fold reduction in recombination rate compared to intraspecific barley crosses. After high-throughput genotyping, the Rym14Hb locus was assigned to a 2Mbp telomeric interval on chromosome 6HS. The co-segregating markers developed in this study can be used for marker-assisted introgression of this locus into barley elite germplasm with a minimum of linkage drag.

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