Quantitative trait loci analysis of flowering time related traits identified in recombinant inbred lines of cowpea (Vigna unguiculata)

Genome ◽  
2013 ◽  
Vol 56 (5) ◽  
pp. 289-294 ◽  
Author(s):  
Mebeasealassie Andargie ◽  
Remy S. Pasquet ◽  
Geoffrey M. Muluvi ◽  
Michael P. Timko
Keyword(s):  
Flowering Time ◽  
Vigna Unguiculata ◽  
Phenotypic Variation ◽  
Recombinant Inbred Lines ◽  
Selection Criterion ◽  
Wild Plants ◽  
Wild Accession ◽  
Flower Opening ◽  
Crop Species ◽  
Long Duration

Flowering time is a major adaptive trait in plants and an important selection criterion in the breeding for genetic improvement of crop species. QTLs for the time of flower opening and days to flower were identified in a cross between a short duration domesticated cowpea (Vigna unguiculata (L.) Walp.) variety, 524B, and a relatively long duration wild accession, 219-01. A set of 159 F7 lines was grown under greenhouse conditions and scored for the flowering time associated phenotypes of time of flower opening and days to flower. Using a LOD threshold of 2.0, putative QTLs were identified and placed on a linkage map consisting of 202 SSR markers and four morphological loci. A total of five QTLs related to the time of flower opening were identified, accounting for 8.8%–29.8% of the phenotypic variation. Three QTLs for days to flower were detected, accounting for 5.7%–18.5% of the phenotypic variation. The major QTL of days to flower and time of flower opening were both mapped on linkage group 1. The QTLs identified in this study provide a strong foundation for further validation and fine mapping for developing an efficient way to restrain the gene flow between the cultivated and wild plants.

scholarly journals Identification of genetic factors controlling domestication-related traits in cowpea (Vigna unguiculata L. Walp)

2017 ◽  
Author(s):  
Sassoum Lo ◽  
María Muñoz-Amatriaín ◽  
Ousmane Boukar ◽  
Ira Herniter ◽  
Ndiaga Cisse ◽  
...  
Keyword(s):  
Flowering Time ◽  
Candidate Genes ◽  
Vigna Unguiculata ◽  
Recombinant Inbred Lines ◽  
Warm Season ◽  
Leaf Length ◽  
Organ Size ◽  
Chromosome 8 ◽  
Nucleotide Polymorphisms ◽  
Pod Shattering

AbstractCowpea (Vigna unguiculata L. Walp) is a warm-season legume with a genetically diverse gene-pool composed of wild and cultivated forms. Cowpea domestication involved considerable phenotypic changes from the wild progenitor, including reduction of pod shattering, increased organ size, and changes in flowering time. Little is known about the genetic basis underlying these changes. In this study, 215 recombinant inbred lines derived from a cross between a cultivated and a wild cowpea accession were used to evaluate nine domestication-related traits (pod shattering, peduncle length, flower color, flowering time, 100-seed weight, pod length, leaf length, leaf width and seed number per pod). A high-density genetic map containing 17,739 single nucleotide polymorphisms was constructed and used to identify 16 quantitative trait loci (QTL) for these nine domestication-related traits. Candidate genes underlying each of those 16 QTL were identified. Four regions with clusters of QTL were identified, including one on chromosome 8 related to increased organ size. This study provides new knowledge of the genomic regions controlling domestication-related traits in cowpea as well as candidate genes underlying those QTL. This information can help to exploit wild relatives in cowpea breeding programs.Key messageThis study identified regions of the cowpea genome that played an important role in cowpea domestication, including a hotspot region for increased organ size

scholarly journals Introgression of Resistance to Leafminer (Liriomyza cicerina Rondani) from Cicer reticulatum Ladiz. to C. arietinum L. and Relationships between Potential Biochemical Selection Criteria

Agronomy ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 57
Author(s):  
Nesrine Chrigui ◽  
Duygu Sari ◽  
Hatice Sari ◽  
Tuba Eker ◽  
Mehmet Fatih Cengiz ◽  
...  
Keyword(s):  
Organic Acids ◽  
Succinic Acid ◽  
Selection Criteria ◽  
Insect Pests ◽  
Recombinant Inbred Lines ◽  
Selection Criterion ◽  
Leaf Miner ◽  
Interspecific Crosses ◽  
Visual Scale ◽  
Resistant Varieties

The chickpea leafminer, Liriomyza cicerina (Rondani), is one of the most destructive insect pests of cultivated chickpea (Cicer arietinum L.) in the Mediterranean region under field conditions. For sustainable and environmentally friendly chickpea production, efforts have been devoted to managing the leafminer via decreasing the use of insecticides. Breeding of new resistant varieties is not only an efficient and practical approach, but also cost-effective and environmentally sensitive. To improve resistant varieties, breeders need reliable biochemical selection criteria that can be used in breeding programs. The first objective was to investigate the possible introgression of resistance to the leafminer from C. reticulatum Ladiz. (resistant) to C. arietinum (susceptible), then, to estimate inheritance of resistance to the leafminer for efficient breeding strategies, and finally, to study organic acid contents as selection criteria. Recombinant inbred lines (RILs) and their parents were evaluated using a visual scale of 1–9 (1 = free from leafminer damage and 9 = mines in more than 91% of the leaflets and defoliation greater than 31%) in the field under natural infestation conditions after the susceptible parent and check had scores of >7 on the visual scale. Superior RILs were found for resistance to the leafminer, and agro-morphological traits indicating that introgression of resistance to leaf miner from C. reticulatum to C. arietinum could be possible using interspecific crosses. The inheritance pattern of resistance to the leafminer in RILs was shown to be quantitative. Organic acids, including oxalic, malic, quinic, tartaric, citric and succinic acids in RILs grown in the field under insect epidemic conditions and in the greenhouse under non-infested conditions were detected by using high performance liquid chromatography (HPLC). In general, organic acids were found to be higher in resistant RILs than susceptible RILs. Path and correlation coefficients showed that succinic acid exhibited the highest direct effects on resistance to the leafminer. Multivariate analyses, including path, correlation and factor analyses suggested that a high level of succinic acid could be used as a potential biochemical selection criterion for resistance to leafminer in chickpea. Resistant RILs with a high seed yield resembling kabuli chickpea can be grown directly in the target environments under leaf miner infestation conditions.

Identification of quantitative trait loci underlying lutein content in soybean seeds across multiple environments

2017 ◽  
Vol 155 (8) ◽  
pp. 1263-1271 ◽  
Author(s):  
W. L. TENG ◽  
W. J. FENG ◽  
J. Y. ZHANG ◽  
N. XIA ◽  
J. GUO ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Phenotypic Variation ◽  
Quantitative Trait ◽  
Average Distance ◽  
Recombinant Inbred Lines ◽  
Chromosome 9 ◽  
Soybean Seeds ◽  
Trait Loci ◽  
Breeding Programmes ◽  
Lutein Content

SUMMARYLutein benefits human health significantly, including that of the eyes, skin and heart. Therefore, increasing lutein content in soybean seeds is an important objective for breeding programmes. However, no information about soybean lutein-related quantitative trait loci (QTL) has been reported, as of 2016. The aim of the present study was to identify QTLs underlying the lutein content in soybean seeds. A population including 129 recombinant inbred lines was developed from the cross between ‘Dongnong46’ (lutein 13·10 µg/g) and ‘L-100’ (lutein 23·96 µg/g), which significantly differed in seed lutein contents. This population was grown in ten environments including Harbin in 2012, 2013, 2014 and 2015; Hulan in 2013, 2014 and 2015; and Acheng in 2013, 2014 and 2015. A total of 213 simple sequence repeat markers were used to construct the genetic linkage map, which covered approximately 3623·39 cM, with an average distance of 17·01 cM between markers. In the present study, eight QTLs associated with lutein content were found initially, which could explain 1·01–19·66% of the observed phenotypic variation in ten different tested environments. The phenotypic contribution of qLU-1 (located near BARC-Satt588 on chromosome 9 (Chr 9; linkage group (LG) K)) was >10% across seven tested environments, while qLU-2 (located near Satt192 of Chr 12 (LG H)) and qLU-3 (located near Satt353 of Chr12 (LGH)) could explain 5–10% of the observed phenotypic variation in more than seven environments, respectively. qLU-5, qLU-6, qLU-7 and qLU-8 could be detected in more than four environments. These eight QTLs were novel, and have considerable potential value for marker-assistant selection of higher lutein content in soybean lines.

scholarly journals Genome-wide association study reveals candidate genes for flowering time in cowpea (Vigna unguiculata [L.] Walp)

2021 ◽  
Author(s):  
Dev Paudel ◽  
Rocheteau Dareus ◽  
Julia Rosenwald ◽  
Maria Munoz-Amatriain ◽  
Esteban Rios
Keyword(s):  
Flowering Time ◽  
Candidate Genes ◽  
Vigna Unguiculata ◽  
Association Studies ◽  
Snp Markers ◽  
Genome Wide Association ◽  
Human Consumption ◽  
Phenotypic Variance ◽  
Genome Wide Association Studies ◽  
Genome Wide

Cowpea (Vigna unguiculata [L.] Walp., diploid, 2n = 22) is a major crop used as a protein source for human consumption as well as a quality feed for livestock. It is drought and heat tolerant and has been bred to develop varieties that are resilient to changing climates. Plant adaptation to new climates and their yield are strongly affected by flowering time. Therefore, understanding the genetic basis of flowering time is critical to advance cowpea breeding. The aim of this study was to perform genome-wide association studies (GWAS) to identify marker trait associations for flowering time in cowpea using single nucleotide polymorphism (SNP) markers. A total of 367 accessions from a cowpea mini-core collection were evaluated in Ft. Collins, CO in 2019 and 2020, and 292 accessions were evaluated in Citra, FL in 2018. These accessions were genotyped using the Cowpea iSelect Consortium Array that contained 51,128 SNPs. GWAS revealed seven reliable SNPs for flowering time that explained 8-12% of the phenotypic variance. Candidate genes including FT, GI, CRY2, LSH3, UGT87A2, LIF2, and HTA9 that are associated with flowering time were identified for the significant SNP markers. Further efforts to validate these loci will help to understand their role in flowering time in cowpea, and it could facilitate the transfer of some of this knowledge to other closely related legume species.

scholarly journals PERANAN TUMBUHAN LIAR DALAM KONSERVASI SERANGGA PENYERBUK ORDO HYMENOPTERA

2016 ◽  
Vol 10 (2) ◽  
pp. 195
Author(s):  
Erniwati Erniwati ◽  
Sih Kahono
Keyword(s):  
Flowering Time ◽  
Food Resources ◽  
Wild Plants ◽  
Cultivated Plants ◽  
Applied Study ◽  
Insect Pollinators ◽  
Cultivated Plant ◽  
Dry Seasons

The role of the wild plants in relation to the conservation of the Indonesian insectpollinators was studied at several areas of Java. Three of direct observationmethods were applied: study of biodiversity and observation on the wild flowersand the insect pollinators as well, and the behaviour of the insects. The flowersof wild plants were relatively smaller and paler in colour, however they were moreattractive to insect pollinators than cultivated plants. Flowering time of the wildplants was mostly during wet seasons, contrary to that of the cultivated plantswhich was mostly during dry seasons. Our observation indicated that these wildplants are the food resources of insect pollinators during wet seasons. Observationdata support the importance of wild plants to supply food to insect pollinatorsduring wet seasons. Management of wild and cultivated plant environments isnecessary to conserve insect pollinators.

The genetic framework of plant breeding

1981 ◽  
Vol 292 (1062) ◽  
pp. 407-419 ◽  
Keyword(s):  
Plant Breeding ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Single Seed Descent ◽  
Crop Species ◽  
Biometrical Genetics ◽  
Mendelian Genetics ◽  
Linkage Disequilibria ◽  
Random Samples

The phenotypic variation that the breeder must manipulate to produce improved genotypes typically contains contributions from both heritable and non-heritable sources as well as from interactions between them. The totality of this variation can be understood only in terms of a methodology such as that of biometrical genetics - an extension of classical Mendelian genetics that retains all of its analytical, interpretative and predictive powers but only in respect of the net or summed effects of all contributing gene loci. In biometrical genetics the statistics that describe the phenotypic distributions are themselves completely described by heritable components based on the known types of gene action and interaction in combination with nonheritable components defined by the statistical properties of the experimental design. Biometrical genetics provides a framework for investigating the genetical basis and justification for current plant breeding strategies that are typified by the production of F 1 hybrids at one extreme and recombinant inbred lines at the other. From the early generations of a cross it can extract estimates of the heritable components of the phenotypic distributions that provide all the information required to interpret the cause of F 1 heterosis and predict the properties of any generation that can subsequently be derived from the cross. Applications to crosses in experimental and crop species show that true overdominance is not a cause of F 1 heterosis, although spurious overdominance arising from linkage disequilibria and non-allelic interactions can be. Predictions of the phenotypic distributions and ranges of recombinant inbred lines that should be extractable from these crosses are confirmed by observations made on random samples of inbred families produced from them by single seed descent. Within these samples, recombinant inbred lines superior to existing inbred lines and their F 1 hybrids are observed with the predicted frequencies.

scholarly journals Convergent Evolution of the Seed Shattering Trait

Genes ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 68 ◽  
Author(s):  
Valerio Di Vittori ◽  
Tania Gioia ◽  
Monica Rodriguez ◽  
Elisa Bellucci ◽  
Elena Bitocchi ◽  
...  
Keyword(s):  
Current Knowledge ◽  
Wild Plants ◽  
Phenotypic Evolution ◽  
Crop Species ◽  
Seed Shattering ◽  
Brassicaceae Species ◽  
Different Types ◽  
Dry Conditions ◽  
Harvesting Efficiency ◽  
The Common

Loss of seed shattering is a key trait in crop domestication, particularly for grain crops. For wild plants, seed shattering is a crucial mechanism to achieve greater fitness, although in the agricultural context, this mechanism reduces harvesting efficiency, especially under dry conditions. Loss of seed shattering was acquired independently in different monocotyledon and dicotyledon crop species by ‘convergent phenotypic evolution’, leading to similar low dehiscent and indehiscent phenotypes. Here, the main aim is to review the current knowledge about seed shattering in crops, in order to highlight the tissue modifications that underlie the convergent phenotypic evolution of reduced shattering in different types of fruit, from the silique of Brassicaceae species, to the pods of legumes and spikes of cereals. Emphasis is given to legumes, with consideration of recent data obtained for the common bean. The current review also discusses to what extent convergent phenotypes arose from parallel changes at the histological and/or molecular levels. For this reason, an overview is included of the main findings relating to the genetic control of seed shattering in the model species Arabidopsis thaliana and in other important crops.

scholarly journals Identification of quantitative trait loci associated with canopy temperature in soybean

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Sumandeep K. Bazzer ◽  
Larry C. Purcell
Keyword(s):  
Quantitative Trait Loci ◽  
Phenotypic Variation ◽  
Quantitative Trait ◽  
Recombinant Inbred Lines ◽  
Canopy Temperature ◽  
Growth And Yield ◽  
Indirect Measure ◽  
Trait Loci ◽  
Genomic Regions ◽  
The Impact

Abstract A consistent risk for soybean (Glycine max L.) production is the impact of drought on growth and yield. Canopy temperature (CT) is an indirect measure of transpiration rate and stomatal conductance and may be valuable in distinguishing differences among genotypes in response to drought. The objective of this study was to map quantitative trait loci (QTLs) associated with CT using thermal infrared imaging in a population of recombinant inbred lines developed from a cross between KS4895 and Jackson. Heritability of CT was 35% when estimated across environments. QTL analysis identified 11 loci for CT distributed on eight chromosomes that individually explained between 4.6 and 12.3% of the phenotypic variation. The locus on Gm11 was identified in two individual environments and across environments and explained the highest proportion of phenotypic variation (9.3% to 11.5%) in CT. Several of these CT loci coincided with the genomic regions from previous studies associated with canopy wilting, canopy temperature, water use efficiency, and other morpho-physiological traits related with drought tolerance. Candidate genes with biological function related to transpiration, root development, and signal transduction underlie these putative CT loci. These genomic regions may be important resources in soybean breeding programs to improve tolerance to drought.

EFFECTIVE FLOWERING TIME VARIATIONS IN UPLAND COTTON (GOSSYPIUM HIRSUTUM) AT DIFFERENT PLANTING DATES AND STAND DENSITIES IN BENIN

2007 ◽  
Vol 43 (2) ◽  
pp. 173-182 ◽  
Author(s):  
EMMANUEL SEKLOKA ◽  
BERNARD HAU ◽  
ERIC GOZÉ ◽  
SYLVIE LEWICKI ◽  
GRÉGOIRE THOMAS ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Flowering Time ◽  
Growth Cycle ◽  
Planting Dates ◽  
Flower Opening ◽  
Late Planting ◽  
Breeding Programmes ◽  
Cycle Lengths ◽  
Time Variations ◽  
The Difference

Effective flowering time in Gossypium hirsutum cotton plants was studied with the aim of enhancing decision making on the best varieties to plant according to the planting date under rainfed cropping conditions. Trials were conducted at two sites in a cotton-growing area of Benin in 2002 and 2003. A split-split plot design with three replicates was used to compare 10 cotton varieties, with different growth cycle lengths and morphology, at three stand densities (42 000, 125 000, 167 000 plants ha−1) and two planting dates (standard planting in June and late planting). The flowering period was characterized by the mean first flower opening date (FF), which is an indicator of flowering earliness, and by the opening date of the last flower giving rise to a first-position boll on fruiting branches (LFP1). Effective flowering time (EFT) was calculated as the difference between LFP1 and FF. EFTs differed markedly in the 10 cotton varieties tested and this parameter could not be predicted on the basis of flowering earliness. Late planting and high planting rates delayed first-flower opening, accelerated last-boll development and reduced the effective flowering time. This latter factor should be taken into account in cotton breeding programmes so that varieties adapted to local rainfall constraints can be recommended to growers while also enhancing crop management sequences.

Sign in / Sign up

Export Citation Format

Share Document