Generation and identification of Brassica alboglabra – Brassica campestris monosomic alien addition lines

Genome ◽  
2013 ◽  
Vol 56 (3) ◽  
pp. 171-177 ◽  
Author(s):  
Xiao-Feng Li ◽  
Shu-Xin Xuan ◽  
Jun-Ling Wang ◽  
Shu-Ling Zhang ◽  
Yan-Hua Wang ◽  
...  
Keyword(s):  
Simple Sequence Repeats ◽  
Morphological Traits ◽  
Brassica Campestris ◽  
Recurrent Parent ◽  
Addition Lines ◽  
Nomenclature System ◽  
Brassica Alboglabra ◽  
Male Gametes ◽  
Simple Sequence ◽  
Alien Addition Lines

Four monosomic alien addition lines (MAALs) for Brassica alboglabra – Brassica campestris were developed through digenomic triploid (ACC) backcrossing with the recurrent parent B. alboglabra (CC). The objectives of this study were to compare morphological traits, microsatellite markers (simple sequence repeats), chromosomal karyotypes, and meiotic behaviors. Based on the new chromosome nomenclature system established for Brassica, we preliminarily identified these MAALs as CC+A1, CC+A3, CC+A6, and CC+A7. Their alien chromosomes were transmittable through both female and male gametes at rates of 11.46%–26.53% and 4.88%–12.90%, respectively.

scholarly journals The Genetic Structure of the Field Pea Landrace “Roveja di Civita di Cascia”

2019 ◽  
Vol 11 (22) ◽  
pp. 6493 ◽  
Author(s):  
Nicoletta Ferradini ◽  
Renzo Torricelli ◽  
Niccolò Terzaroli ◽  
Emidio Albertini ◽  
Luigi Russi
Keyword(s):  
Genetic Structure ◽  
Pisum Sativum ◽  
Simple Sequence Repeats ◽  
Morphological Traits ◽  
Central Italy ◽  
Marginal Land ◽  
Local Varieties ◽  
On Farm ◽  
Simple Sequence ◽  
Private Alleles

“Roveja di Civita di Cascia” is a landrace of Pisum sativum grown in marginal land habitats of the Apennines, Central Italy, and is one of the eleven herbaceous crop landraces listed in the Regional Register of local varieties. The objective of the present paper was to assess its genetic structure using 62 morphological traits and five microsatellites. As many as 55 traits showed significant differences with the control entries (P. sativum subsp. sativum var. arvense and var. sativum). We tested P. sativum Simple Sequence Repeats (SSRs) for their transferability to “Roveja”, and found that only 12 out of 35 performed well. Of these, we demonstrated that five were sufficient to assess the genetic structure of this landrace, characterized by several private alleles, differentiating it from Paladio and Bluemoon, which were used as controls. Phenotypic and genotypic data evidenced a genetic structure based on a blend of several pure-bred lines. The sustainability of on-farm landrace conservation is discussed.

scholarly journals Genetic Diversity in <i>Jatropha platyphylla</i> Accessions Based on Morphological Traits and Inter-Simple Sequence Repeats Molecular Markers

2021 ◽  
Vol 12 (11) ◽  
pp. 1658-1672
Author(s):  
Edith Salazar-Villa ◽  
Lilia Alcaraz-Meléndez ◽  
Josefina León-Félix ◽  
José Basilio Heredia ◽  
Federico Soto-Landeros ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Morphological Traits ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

scholarly journals Morphological and Molecular Characterization of Iranian Wild Blackberry Species Using Multivariate Statistical Analysis and Inter-Simple Sequence Repeats (ISSR) Markers 

2021 ◽  
Author(s):  
Ali Gharaghani ◽  
Mehdi Garazhian ◽  
Saeid Eshghi ◽  
Ahmad Tahmasebi
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeats ◽  
Morphological Traits ◽  
Diversity Index ◽  
Issr Markers ◽  
Multivariate Statistical ◽  
Principal Coordinates ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence ◽  
Issr Primers

Abstract This study was carried out to estimate the genetic diversity and relationships of 74 Iranian blackberry genotypes assigned to 5 different species using inter-simple sequence repeats (ISSR) marker analysis and morphological trait characterization. Sixteen traits including phenological, vegetative and reproductive attributes were recorded, and 10 ISSR primers were screened. Results showed that yield and leaf width have the highest and lowest genetic diversity, (diversity index = 62.57 and 13.74), respectively. Flowering and ripening date recorded as traits having the strongest correlations (r = 0.98). The selected 10 ISSR primers produced a total of 161 amplified fragments (200 to 3500 bp) of which 113 were polymorphic. The highest, lowest and average PIC values were 0.53, 0.38 and 0.44, respectively. Principle component analysis (PCA) based on morphological traits showed that the first six components explained 84.9% of the variations of traits studied, whilst the principal coordinate analysis (PCoA) based on ISSR data implied the first eight principal coordinates explained 67.06% of the total variation. Cluster analysis based on morphological traits and ISSR data classified all genotypes into two and three major groups, respectively, and the distribution pattern of genotypes was mainly based on species and the geographic origins.

scholarly journals Linkage of RAPD and SSR Markers to Thorniness and Floricane Fruiting in Blackberry

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1147B-1147
Author(s):  
Eric Stafne ◽  
John Clark ◽  
Kim Lewers
Keyword(s):  
Molecular Markers ◽  
Random Sample ◽  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Marker Assisted Selection ◽  
Morphological Traits ◽  
Rapd Markers ◽  
Randomly Amplified Polymorphic Dna ◽  
Simple Sequence

Molecular markers have been used previously to identify linkages to important traits of interest. In this study two marker types, randomly amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR), were used to find molecular markers linked to two morphological traits in blackberry (Rubus L. subgenus Rubus). Thorniness and floricane fruiting are both qualitative, recessive traits that are inherited tetrasomically. A cross of `Prime-Jim'® × `Arapaho' was made to create a population that segregated for the two traits. A random sample of 98 plants from a population of 200 were assayed to find molecular markers that co-segregate with the two traits. Three putative markers were identified for the floricane fruiting trait (two SSRs and one RAPD; χ2 = 4.09 to 9.99, P < 0.001 to 0.043). Five potential RAPD markers were found for the thorny trait (χ2 = 3.88 to 10.23, P < 0.001 to 0.048). Identification of markers linked to these traits could potentially be useful in marker-assisted selection.

Polymorphism revealed by simple sequence repeats

1996 ◽  
Vol 1 (7) ◽  
pp. 215-222 ◽  
Author(s):  
W POWELL ◽  
G MACHRAY ◽  
J PROVAN
Keyword(s):  
Simple Sequence Repeats ◽  
Simple Sequence

scholarly journals Alien introgression and morpho-agronomic characterization of diploid progenies of Solanum lycopersicoides monosomic alien addition lines (MAALs) toward pre-breeding applications in tomato (S. lycopersicum)

2021 ◽  
Author(s):  
Puneet Kaur Mangat ◽  
Junghyun Shim ◽  
Ritchel B. Gannaban ◽  
Joshua J. Singleton ◽  
Rosalyn B. Angeles-Shim
Keyword(s):  
Chromosome Complement ◽  
Introgression Lines ◽  
Addition Lines ◽  
Solanum Lycopersicoides ◽  
Drought Tolerant ◽  
Use Efficiency ◽  
Tomato Breeding ◽  
Chromosome Segments ◽  
Alien Addition Lines

Abstract Key message Alien introgressions that were captured in the genome of diploid plants segregating from progenies of monosomic alien addition lines of S. lycopersicoides confer novel phenotypes with commercial and agronomic value in tomato breeding. Abstract Solanum lycopersicoides is a wild relative of tomato with a natural adaptation to a wide array of biotic and abiotic challenges. In this study, we identified and characterized diploid plants segregating from the progenies of monosomic alien addition lines (MAALs) of S. lycopersicoides to establish their potential as donors in breeding for target trait improvement in tomato. Molecular genotyping identified 28 of 38 MAAL progenies having the complete chromosome complement of the cultivated tomato parent and limited chromosome introgressions from the wild S. lycopersicoides parent. Analysis of SSR and indel marker profiles identified 34 unique alien introgressions in the 28 MAAL-derived introgression lines (MDILs) in the genetic background of tomato. Conserved patterns of alien introgressions were detected among sibs of MDILs 2, 3, 4 and 8. Across MDILs, a degree of preferential transmission of specific chromosome segments was also observed. Morphologically, the MDILs closely resembled the cultivated tomato more than S. lycopersicoides. The appearance of novel phenotypes in the MDILs that are lacking in the cultivated parent or the source MAALs indicates the capture of novel genetic variation by the diploid introgression lines that can add commercial and agronomic value to tomato. In particular, screening of representative MDILs for drought tolerance at the vegetative stage identified MDIL 2 and MDIL 11III as drought tolerant based on visual scoring. A regulated increase in stomatal conductance of MDIL 2 under drought stress indicates better water use efficiency that allowed it to survive for 7 days under 0% moisture level.

scholarly journals Implications of genome simple sequence repeats signature in 98 Polyomaviridae species

3 Biotech ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rezwanuzzaman Laskar ◽  
Md Gulam Jilani ◽  
Safdar Ali
Keyword(s):  
Simple Sequence Repeats ◽  
Simple Sequence

Instability of simple sequence repeats in a mammalian cell line

1994 ◽  
Vol 3 (2) ◽  
pp. 253-256 ◽  
Author(s):  
Rosann A. Farber ◽  
Thomas D. Petes ◽  
Margaret Dominska ◽  
Sarah S. Hudgens ◽  
R.Michael Liskay
Keyword(s):  
Cell Line ◽  
Mammalian Cell ◽  
Simple Sequence Repeats ◽  
Mammalian Cell Line ◽  
Simple Sequence
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