Wheat CBF gene family: identification of polymorphisms in the CBF coding sequence

Genome ◽  
2012 ◽  
Vol 55 (12) ◽  
pp. 865-881 ◽  
Author(s):  
Sara Mohseni ◽  
Hua Che ◽  
Zakia Djillali ◽  
Estelle Dumont ◽  
Joseph Nankeu ◽  
...  
Keyword(s):  
Low Temperature ◽  
Gene Family ◽  
Hexaploid Wheat ◽  
Temperature Tolerance ◽  
Freezing Stress ◽  
Coding Region ◽  
Additional Information ◽  
Low Temperature Tolerance ◽  
Cbf Genes ◽  
Cbf Transcription Factor

Expression of cold-regulated genes needed for protection against freezing stress is mediated, in part, by the CBF transcription factor family. Previous studies with temperate cereals suggested that the CBF gene family in wheat was large, and that CBF genes were at the base of an important low temperature tolerance trait. Therefore, the goal of our study was to identify the CBF repertoire in the freezing-tolerant hexaploid wheat cultivar Norstar, and then to examine if the coding region of CBF genes in two spring cultivars contain polymorphisms that could affect the protein sequence and structure. Our analyses reveal that hexaploid wheat contains a complex CBF family consisting of at least 65 CBF genes of which 60 are known to be expressed in the cultivar Norstar. They represent 27 paralogous genes with 1–3 homeologous copies for the A, B, and D genomes. The cultivar Norstar contains two pseudogenes and at least 24 additional proteins having sequences and (or) structures that deviate from the consensus in the conserved AP2 DNA-binding and (or) C-terminal activation-domains. This suggests that in cultivars such as Norstar, low temperature tolerance may be increased through breeding of additional optimal alleles. The examination of the CBF repertoire present in the two spring cultivars, Chinese Spring and Manitou, reveals that they have additional polymorphisms affecting conserved positions in these domains. Understanding the effects of these polymorphisms will provide additional information for the selection of optimum CBF alleles in Triticeae breeding programs.

scholarly journals PsCor413pm2, a Plasma Membrane-Localized, Cold-Regulated Protein from Phlox subulata, Confers Low Temperature Tolerance in Arabidopsis

2018 ◽  
Vol 19 (9) ◽  
pp. 2579 ◽  
Author(s):  
Aimin Zhou ◽  
Enhui Liu ◽  
He Li ◽  
Yang Li ◽  
Shuang Feng ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Low Temperature ◽  
Temperature Stress ◽  
Transgenic Arabidopsis ◽  
Temperature Tolerance ◽  
Low Temperature Stress ◽  
Wild Type ◽  
Low Temperature Tolerance ◽  
Isolation And Characterization ◽  
Cbf Genes

Low temperature stress adversely affects plant growth and development. Isolation and characterization of cold response genes from cold-tolerant plants help to understand the mechanism underlying low temperature tolerance. In this study, PsCor413pm2, a cold-regulated (COR) gene isolated from Phlox subulata, was transferred to Arabidopsis plants to investigate its function. Real-time quantitative PCR analysis revealed that PsCor413pm2 expression was induced by cold. Subcellular localization revealed that the PsCor413pm2-green fluorescent protein (GFP) fusion protein localized to the plasma membrane in tobacco and Arabidopsis plants. Furthermore, overexpression of PsCor413pm2 in Arabidopsis plants enhanced tolerance to low temperature stress. Transgenic Arabidopsis roots had more influx of Ca2+ after a cold shock than wild-type plants, as shown using non-invasive micro-test technology (NMT). Moreover, the transcription abundance of five COR and two C-repeat (CRT) binding factor (CBF) genes in transgenic Arabidopsis plants was higher than that in the wild-type plants under cold stress. Taken together, our results suggest that overexpression of PsCor413pm2 enhances low temperature tolerance in Arabidopsis plants by affecting Ca2+ flux and the expression of stress-related COR and CBF genes.

scholarly journals Low temperature tolerance of human embryonic stem cells

2006 ◽  
pp. 124-129 ◽  
Author(s):  
Boon Chin Heng ◽  
Kumar Jayaseelan Vinoth ◽  
Hua Liu ◽  
Manoor Prakash Hande ◽  
Tong Cao
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Low Temperature ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Temperature Tolerance ◽  
Low Temperature Tolerance

scholarly journals Joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259455
Author(s):  
QianQian Zhuang ◽  
Shaopeng Chen ◽  
ZhiXin Jua ◽  
Yue Yao
Keyword(s):  
Signal Transduction ◽  
Low Temperature ◽  
Temperature Tolerance ◽  
Differentially Expressed ◽  
Metabolomic Analysis ◽  
Temperature Changes ◽  
Low Temperature Tolerance ◽  
Content Ratio ◽  
Temperature Damage ◽  
Hosta Ventricosa

Hosta ventricosa is a robust ornamental perennial plant that can tolerate low temperatures, and which is widely used in urban landscaping design in Northeast China. However, the mechanism of cold-stress tolerance in this species is unclear. A combination of transcriptomic and metabolomic analysis was used to explore the mechanism of low-temperature tolerance in H. ventricosa. A total of 12 059 differentially expressed genes and 131 differentially expressed metabolites were obtained, which were mainly concentrated in the signal transduction and phenylpropanoid metabolic pathways. In the process of low-temperature signal transduction, possibly by transmitting Ca2+ inside and outside the cell through the ion channels on the three cell membranes of COLD, CNGCs and CRLK, H. ventricosa senses temperature changes and stimulates SCRM to combine with DREB through the MAPK signal pathway and Ca2+ signal sensors such as CBL, thus strengthening its low-temperature resistance. The pathways of phenylpropanoid and flavonoid metabolism represent the main mechanism of low-temperature tolerance in this species. The plant protects itself from low-temperature damage by increasing its content of genistein, scopolentin and scopolin. It is speculated that H. ventricosa can also adjust the content ratio of sinapyl alcohol and coniferyl alcohol and thereby alter the morphological structure of its cell walls and so increase its resistance to low temperatures.When subjected to low-temperature stress, H. ventricosa perceives temperature changes via COLD, CNGCs and CRLK, and protection from low-temperature damage is achieved by an increase in the levels of genistein, scopolentin and scopolin through the pathways of phenylpropanoid biosynthesis and flavonoid biosynthesis.

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