Identification of rpaP1-5 and rpaP2-6 genes encoding two additional variants of the 60S acidic ribosomal proteins of Schizosaccharomyces pombe

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 205-207 ◽  
Author(s):  
Christine Bonnet ◽  
Eric Perret ◽  
Odile Bonnin ◽  
André Picard ◽  
Daniel Caput ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Schizosaccharomyces Pombe ◽  
Key Words ◽  
Fission Yeast ◽  
Sequence Comparison ◽  
Ribosomal Proteins ◽  
Genes Encoding ◽  
Acidic Ribosomal Protein

In the fission yeast, four genes (rpaP1-1, rpaP1-3,rpaP2-2, and rpaP2-4) encoding two variants of the RpaP1 and RpaP2 ribosomal proteins (rp) have been characterized. We have identified cDNA for additional variants called RpaP1.5 and RpaP2.6. Sequence comparison suggests that RpaP1.5 diverged before RpaP1.1 and RpaP1.3 and that RpaP2.6 is closer to RpaP2.2 than to RpaP2.4. The corresponding genes, rpaP1-5 and rpaP2-6, are transcribed coordinately with other rp genes. Key words: acidic ribosomal protein, RpaP1.5, RpaP2.6, rpaP1-5, rpaP2-6, variants.

Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

Ribosomal proteins S0 and S21 are involved in the stability of 18S rRNA in fission yeast, Schizosaccharomyces pombe

2003 ◽  
Vol 311 (4) ◽  
pp. 942-947 ◽  
Author(s):  
Manabu Sato ◽  
Cheong Jit Kong ◽  
Hideji Yoshida ◽  
Taro Nakamura ◽  
Akira Wada ◽  
...  
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Ribosomal Proteins ◽  
18S Rrna ◽  
The Stability

scholarly journals Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3.

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905 ◽  
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

scholarly journals SSB, Encoding a Ribosome-Associated Chaperone, Is Coordinately Regulated with Ribosomal Protein Genes

1999 ◽  
Vol 181 (10) ◽  
pp. 3136-3143 ◽  
Author(s):  
Nelson Lopez ◽  
John Halladay ◽  
William Walter ◽  
Elizabeth A. Craig
Keyword(s):  
Heat Shock ◽  
Ribosomal Protein ◽  
Ribosomal Proteins ◽  
Chain Complex ◽  
Mrna Levels ◽  
Ribosomal Protein Genes ◽  
Genes Encoding ◽  
Synthetic Capacity ◽  
Translational Apparatus ◽  
Temperature Upshift

ABSTRACT Genes encoding ribosomal proteins and other components of the translational apparatus are coregulated to efficiently adjust the protein synthetic capacity of the cell. Ssb, a Saccharomyces cerevisiae Hsp70 cytosolic molecular chaperone, is associated with the ribosome-nascent chain complex. To determine whether this chaperone is coregulated with ribosomal proteins, we studied the mRNA regulation of SSB under several environmental conditions. Ssb and the ribosomal protein rpL5 mRNAs were up-regulated upon carbon upshift and down-regulated upon amino acid limitation, unlike the mRNA of another cytosolic Hsp70, Ssa. Ribosomal protein and Ssb mRNAs, like many mRNAs, are down-regulated upon a rapid temperature upshift. The mRNA reduction of several ribosomal protein genes and Ssb was delayed by the presence of an allele, EXA3-1, of the gene encoding the heat shock factor (HSF). However, upon a heat shock theEXA3-1 mutation did not significantly alter the reduction in the mRNA levels of two genes encoding proteins unrelated to the translational apparatus. Analysis of gene fusions indicated that the transcribed region, but not the promoter of SSB, is sufficient for this HSF-dependent regulation. Our studies suggest that Ssb is regulated like a core component of the ribosome and that HSF is required for proper regulation of SSB and ribosomal mRNA after a temperature upshift.

Molecular cloning of a ribosomal protein gene from the fission yeast Schizosaccharomyces pombe

1986 ◽  
Vol 10 (5) ◽  
pp. 365-370 ◽  
Author(s):  
Roswitha Nischt ◽  
Eduardo Th�roff ◽  
Norbert F. K�ufer
Keyword(s):  
Ribosomal Protein ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Molecular Cloning ◽  
Protein Gene ◽  
Ribosomal Protein Gene
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