A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 137-142 ◽  
Author(s):  
B Moury ◽  
S Pflieger ◽  
A Blattes ◽  
V Lefebvre ◽  
A Palloix
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Bulked Segregant Analysis ◽  
Rapd Marker ◽  
Dominant Gene ◽  
Caps Marker ◽  
Hypersensitive Resistance ◽  
Pcr Marker ◽  
Tomato Spotted Wilt ◽  
Wide Range ◽  
Wilt Virus

The hypersensitive resistance to tomato spotted wilt virus (TSWV) in pepper is determined by a single dominant gene (resistant allele: Tsw) in several Capsicum chinense genotypes. In order to facilitate the selection for this resistance, four RAPD (among 250 10-mer primers tested) were found linked to the Tsw locus using the bulked segregant analysis and 153 F2 individuals. A close RAPD marker was converted into a codominant cleaved amplified polymorphic sequence (CAPS) using specific PCR primers and restriction enzymes. This CAPS marker is tightly linked to Tsw (0.9 ± 0.6 cM) and is helpful for marker-assisted selection in a wide range of genetic intercrosses. Key words: Capsicum sp., PCR marker, bulked segregant analysis, Tospovirus, resistance gene.

scholarly journals Importance and epidemiology of tomato spotted wilt virus

Biljni lekar ◽  
2021 ◽  
Vol 49 (2) ◽  
pp. 148-157
Author(s):  
Marina Ćuk ◽  
Zagorka Savić ◽  
Renata Iličić ◽  
Ferenc Bagi
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Frankliniella Occidentalis ◽  
Wide Distribution ◽  
Plant Viruses ◽  
Agricultural Crops ◽  
Agricultural Producers ◽  
Larval Stages ◽  
Tomato Spotted Wilt ◽  
Wide Range ◽  
Wilt Virus

Tomato spotted wilt virus (TSWV) is the most economically important plant viruses from genus Tospovirus. It has a polyphagous character and infects a wide range of very significant agricultural crops. Vectors of viruses are insects from order Thysanoptera (Thripidae) and till know eight species are known to transmit tospoviruses of which Frankliniella occidentalis is considered to be economically most important vector. TSWV is transmitted by thrips in a persistent and propagative manner. Relationship between vector and TSWV is very specific because vectors acquire the virus in the larval stages, while imago plays a key role in transmission of the virus. TSWV causes wide range of symptoms depending on host plant, external environmental conditions and type of viruses. In addition to affecting the fruit quality of cultivated crops, greatly reduces the yield to agricultural producers. Tomato is the most commonly attacked by TSWV, and after the symptoms manifested on leaves in the form of a bronze color, the virus was name. Protection of agricultural crops is very challenging and difficult due to wide distribution of viruse vectors, their hidden way of life as well as wide range of TSWV hosts.

Resistance-breaking tomato spotted wilt virus variant that recently occurred in pepper in South Korea is a genetic reassortant

Plant Disease ◽  
2021 ◽  
Author(s):  
Sun-Jung Kwon ◽  
Young-Eun Cho ◽  
Oh-Hun Kwon ◽  
Hyung-Gon Kang ◽  
Jang-Kyun Seo
Keyword(s):  
South Korea ◽  
Genome Sequence ◽  
Tomato Spotted Wilt Virus ◽  
Dominant Gene ◽  
Potential Threat ◽  
Viral Pathogen ◽  
South Korean ◽  
Tomato Spotted Wilt ◽  
Resistance Breaking ◽  
Wilt Virus

Tomato spotted wilt virus (TSWV) is a destructive viral pathogen in various crops, including pepper. While the single dominant gene Tsw has been utilized in pepper breeding to confer resistance to TSWV, the occurrence of TSWV variants that overcome Tsw-mediated resistance has been reported in various countries after several years of growing resistant cultivars. In this study, we determined the complete genome sequence of a resistance-breaking TSWV variant (TSWV-YI) that recently emerged in pepper in South Korea. TSWV-YI infected all the resistant pepper cultivars tested. The phylogenetic and recombination analyses of the complete TSWV-YI genome sequence showed that it is a reassortant that acquired its L and M RNA segments from the existing South Korean TSWV population and its S RNA in an isolate from another country. Given that TSWV-YI is a resistance-breaking variant, it appears that reassortment of the S RNA led to the emergence of this variant that breaks the Tsw gene in pepper grown in South Korea. Our results suggest that resistance-breaking TSWV variants are a potential threat to pepper production in South Korea and that strategies to manage these variants should be developed to ensure sustainable pepper production.

scholarly journals Laboratory diagnostics of tomato spotted wilt virus by PCR

2021 ◽  
Author(s):  
O.N. Morozova ◽  
D. D. Zvyagintseva ◽  
O.O. Beloshapkina
Keyword(s):  
Self Assembly ◽  
Tomato Spotted Wilt Virus ◽  
Test System ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Laboratory Diagnostics ◽  
Tomato Spotted Wilt ◽  
Specificity And Sensitivity ◽  
Wide Range ◽  
Wilt Virus

AbstractTomato spotted wilt virus is a widespread and harmful virus. It affects a wide range of host plants. The outward signs of TSWV lesions are different on different cultures. For the early diagnosis of TSWV, molecular diagnostic methods such as PCR must be used. In the course of this work, primers were developed for the diagnosis of tomato bronzing virus by real-time PCR and classical PCR. We also compared the specificity and sensitivity of various test systems for the diagnosis of tomato bronzing virus. In the course of this comparison, it was found that the self-assembly test system and the Syntol test system can be recommended for laboratory diagnostics of tomato spotted wilt virus.

scholarly journals Non-Structural Protein NSm of Tomato Spotted Wilt Virus Is an Avirulence Factor Recognized by Resistance Genes of Tobacco and Tomato via Different Elicitor Active Sites

Viruses ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 660 ◽  
Author(s):  
Changjun Huang ◽  
Yong Liu ◽  
Haiqin Yu ◽  
Cheng Yuan ◽  
Jianmin Zeng ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Active Sites ◽  
Tomato Spotted Wilt Virus ◽  
Dominant Gene ◽  
Structural Protein ◽  
Viral Pathogens ◽  
Tomato Spotted Wilt ◽  
Movement Function ◽  
Wilt Virus ◽  
Avr Gene

Tomato spotted wilt virus (TSWV) is one of the most destructive viral pathogens of plants. Recently, a single dominant gene conferring complete resistance to TSWV (RTSW) was identified in Nicotina alata and introgressed into cultivated tobacco (N. tabacum). However, whether the TSWV carries an avirulence (Avr) factor directed against RTSW remains obscure. In the present study, we identified the non-structural protein (NSm), the movement protein of TSWV, which is an RTSW-specific Avr factor, by using two different transient expression systems. Using amino acid (aa) substitution mutants, we demonstrated the ability to induce RTSW-mediated hypersensitive response (HR) of NSm is independent of its movement function. Moreover, key substitutions (C118Y and T120N), a 21-aa viral effector epitope, and different truncated versions of NSm, which are responsible for the recognition of the Sw-5b resistance gene of tomato, were tested for their ability to trigger HR to TSWV in tobacco. Together, our results demonstrated that RTSW-mediated resistance is triggered by NSm in the same way as by Sw-5b, however, via different elicitor active sites. Finally, an Avr gene-based diagnostic approach was established and used to determine the presence and effectiveness of resistance genes in tobacco.

FEEDING PREFERENCES OF THE WESTERN FLOWER THRIPS, FRANKLINIELLA OCCIDENTALIS (PERGANDE) (THYSANOPTERA: THRIPIDAE), AND INCIDENCE OF TOMATO SPOTTED WILT VIRUS AMONG CULTIVARS OF FLORIST’S CHRYSANTHEMUM

1990 ◽  
Vol 122 (6) ◽  
pp. 1111-1117 ◽  
Author(s):  
A.B. Broadbent ◽  
J.A. Matteoni ◽  
W.R. Allen
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Frankliniella Occidentalis ◽  
Feeding Activity ◽  
Western Flower Thrips ◽  
Feeding Damage ◽  
Flower Thrips ◽  
Tomato Spotted Wilt ◽  
Wide Range ◽  
Growth Room ◽  
Wilt Virus

AbstractA wide range in feeding damage as defined by leaf scars among 27 cultivars of florist’s chrysanthemum was apparent for the western flower thrips, Frankliniella occidentalis (Pergande). Assessments based on numbers of feeding scars or ranking of cultivars on the basis of the leaf area damaged by feeding were in close agreement. Cultivars with the most feeding damage under growth room conditions, such as cvs. White Marble and Polaris, were the same as those in plots within a naturally infested commercial greenhouse. Under growth room conditions, a 2- to 4-week exposure of plants to a population of thrips was sufficient to evaluate feeding damage. Closely related cultivars had similar levels of feeding damage, and foliage of yellow-flowered cultivars was significantly more attractive to thrips for feeding than foliage of white-flowered sister cultivars during the pre-bloom period. There was a low correlation between feeding damage and incidence of the tomato spotted wilt virus among cultivars in both growth room and greenhouse tests, indicating that virus susceptibility of cultivars was epidemiologically more significant than the feeding activity of western flower thrips.

scholarly journals A New Dominantly Inherited Source of TSWV Resistance in Tomato Derived from L. chilense, which Resists Isolates that Overcome Sw-5

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 991A-991 ◽  
Author(s):  
Mikel R. Stevens ◽  
John W. Scott ◽  
John J. Cho ◽  
Bradley D. Geary ◽  
Frederic D. Memmott
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Single Gene ◽  
Dominant Gene ◽  
Field Studies ◽  
Limiting Factor ◽  
Tomato Production ◽  
Tomato Spotted Wilt ◽  
Greenhouse Screening ◽  
Production Areas ◽  
Wilt Virus

Tomato spotted wilt virus (TSWV), a tospovirus, is a thrips-vectored disease infecting more than 1000 species of both monocots and dicots, including many species of agriculture importance. TSWV is the limiting factor for tomato (Lycopersicum esculentum Mill.) production in several areas of the world. For a number of years, the Sw-5 gene (derived from L. peruvianum Mill.) has provided acceptable control of this disease. Recently, Sw-5 derived resistance has been overcome by virulent pathogen isolate(s) in tomato production areas such as Spain and Italy. In earlier studies, we identified a potential new source of resistance to TSWV derived from L. chilense Dun. accession LA 1938. In a set of recent field studies, it was demonstrated that this putative new source of resistance was highly resistant to TSWV in Hawaii, Florida/Georgia, and South Africa. Furthermore, greenhouse screening trials have clearly demonstrated that the L. chilense source of TSWV resistance is resistant to isolates that overcome tomatoes homozygous for Sw-5. In these same greenhouse and field studies, there is uniform evidence that this resistance is dominant. Subsequent greenhouse studies suggest that this resistance is controlled by a single gene. Studies have been initiated to verify the inheritance of the gene(s) and to develop linked molecular markers. Furthermore, studies are under way in Australia to test this resistance on non-TSWV tospoviruses. If the data demonstrate that this is a single dominant gene we suggest this gene be designated Sw-7.

scholarly journals Current Status and Potential of RNA Interference for the Management of Tomato Spotted Wilt Virus and Thrips Vectors

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 320
Author(s):  
Alexander Nilon ◽  
Karl Robinson ◽  
Hanu R. Pappu ◽  
Neena Mitter
Keyword(s):  
Rna Interference ◽  
Tomato Spotted Wilt Virus ◽  
Cultural Practices ◽  
Management Strategies ◽  
Current Status ◽  
Horticultural Crops ◽  
Yield And Quality ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Tomato spotted wilt virus (TSWV) is the type member of the genus Orthotospovirus in the family Tospoviridae and order Bunyavirales. TSWV, transmitted by several species of thrips, causes significant disease losses to agronomic and horticultural crops worldwide, impacting both the yield and quality of the produce. Management strategies include growing virus-resistant cultivars, cultural practices, and managing thrips vectors through pesticide application. However, numerous studies have reported that TSWV isolates can overcome host-plant resistance, while thrips are developing resistance to pesticides that were once effective. RNA interference (RNAi) offers a means of host defence by using double-stranded (ds) RNA to initiate gene silencing against invading viruses. However, adoption of this approach requires production and use of transgenic plants and thus limits the practical application of RNAi against TSWV and other viruses. To fully utilize the potential of RNAi for virus management at the field level, new and novel approaches are needed. In this review, we summarize RNAi and highlight the potential of topical or exogenous application of RNAi triggers for managing TSWV and thrips vectors.

Lipid in a Purified Preparation of Tomato Spotted Wilt Virus

Nature ◽  
1964 ◽  
Vol 203 (4945) ◽  
pp. 671-672 ◽  
Author(s):  
RUPERT J. BEST ◽  
GERARD F. KATEKAR
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Tomato Spotted Wilt ◽  
Wilt Virus
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