Development of a CAPS marker for the Pvr4 locus: A tool for pyramiding potyvirus resistance genes in pepper

Genome ◽  
1999 ◽  
Vol 42 (6) ◽  
pp. 1111-1116 ◽  
Author(s):  
Carole Caranta ◽  
Arnaud Thabuis ◽  
Alain Palloix
Keyword(s):  
Resistance Genes ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Marker Assisted Selection ◽  
Fragment Length Polymorphism ◽  
Bulked Segregant Analysis ◽  
Amplified Fragment Length Polymorphism ◽  
Aflp Markers ◽  
Caps Marker ◽  
Using Data

The Pvr4 resistance gene in pepper confers a complete resistance to the three pathotypes of potato virus Y (PVY) and to pepper mottle virus (PepMoV). In order to use this gene in a marker-assisted selection (MAS) program and to permit the pyramiding of several potyvirus resistance genes in the same cultivar, tightly linked amplified fragment length polymorphism (AFLP) markers were obtained by the bulked segregant analysis method. Eight linked AFLP markers were mapped in an interval from 2.1 ± 0.8 to 13.8 ± 2.9 cM around this locus. The closest codominant AFLP marker was converted into a codominant CAPS (cleaved amplified polymorphic sequence) marker using data from the alignment of the two allele sequences. We have further characterized the relevance of the CAPS marker for MAS programs in different pepper breeding lines.Key words: amplified fragment length polymorphism markers, bulked segregant analysis, Capsicum annuum, marker-assisted selection, potyvirus resistance.

scholarly journals Identification of Amplified Fragment Length Polymorphism Markers Linked to Gummy Stem Blight (Didymella bryoniae) Resistance in Melon (Cucumis melo L.) PI 420145

HortScience ◽  
2009 ◽  
Vol 44 (1) ◽  
pp. 32-34 ◽  
Author(s):  
Joseph N. Wolukau ◽  
Xiaohui Zhou ◽  
JinFeng Chen
Keyword(s):  
Molecular Markers ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Dominant Gene ◽  
Amplified Fragment Length Polymorphism ◽  
Aflp Markers ◽  
Didymella Bryoniae ◽  
Stem Blight ◽  
Gummy Stem Blight

Gummy stem blight (GSB) caused by the ascomycete fungus Didymella bryoniae (Auersw.) Rehm is an important disease of melon. Molecular markers linked to resistance would be useful for melon breeding programs. The amplified fragment length polymorphism (AFLP) technique and bulk segregant analysis were used to identify molecular markers linked to the resistance of melon to Didymella bryoniae. Segregation analysis of F2 progeny from a cross of PI 420145, a resistant line, and PI 136170, a susceptible line, showed that resistance to GSB was controlled by a dominant gene. One AFLP marker, E-TG/M-CTC200, was identified that is tightly linked to GSB resistance gene at a distance of 2.0 cM. To our best knowledge, this is the first report of AFLP markers linked to GSB resistance in melon. The identification of AFLP markers provides a step toward the use of marker-assisted selection and the characterization of the gene encoding resistance to GSB in melon.

scholarly journals Analysis of Genetic Heterogeneity in Chlamydia trachomatis Clinical Isolates of Serovars D, E, and F by Amplified Fragment Length Polymorphism

2000 ◽  
Vol 38 (9) ◽  
pp. 3463-3466 ◽  
Author(s):  
Servaas A. Morré ◽  
Jacobus M. Ossewaarde ◽  
Paul H. M. Savelkoul ◽  
Jeroen Stoof ◽  
Chris J. L. M. Meijer ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Length Polymorphism ◽  
Genetic Heterogeneity ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Aflp Markers ◽  
Clinical Isolates ◽  
Insight Into

Amplified fragment length polymorphism (AFLP) fingerprinting of clinical isolates of Chlamydia trachomatis serovars D, E, and F showed a low percentage of genetic heterogeneity, but clear differences were found. Isolates from index patients and partners had identical AFLP patterns and AFLP markers. Characterization of these AFLP markers could give more insight into the differences in virulence and clinical course of C. trachomatis infections.

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