A twenty strain survey and backcross localization of the erythrocytic GTP concentration determining locus Gtpc on mouse chromosome 9

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 447-452 ◽  
Author(s):  
G J Wiebe ◽  
E Fung ◽  
F G Biddle ◽  
F F Snyder
Keyword(s):  
Microsatellite Markers ◽  
Mouse Chromosome ◽  
Gene Order ◽  
Inbred Strains ◽  
Genetic Distances ◽  
Chromosome 9 ◽  
Atp Concentration ◽  
Inbred Strains Of Mice ◽  
Significant Difference ◽  
Kidney Liver

Erythrocyte nucleotide concentrations were surveyed among 20 inbred strains of mice in order to further assess the variability in GTP concentration. There was no significant difference in erythrocytic ATP concentration (Scheffé's test at P = 0.01), 678-1154 nmol/mL packed cells, among the strains surveyed. Two groups were distinguishable with respect to erythrocytic GTP concentration, 8 strains having high GTP, 215 ± 44 nmole/mL packed cells, and 12 strains having low GTP, 34 ± 12 nmole/mL packed cells. The erythrocytic GTP concentration determining trait Gtpc was previously shown to be linked to transferrin, Trf, on chromosome 9. Analysis of 232 [(B6 × WB) F1 × B6] backcross individuals for Gtpc and 8 microsatellite markers restricted the localization of Gtpc to a 5.6 ± 2.1 cM region. The gene order and genetic distances in cM ± SE are: (D9Mit14) 0.4 ± 0.4 (D9Mit24) 1.7 ± 0.8 (Gtpc, D9Mit51, D9Mit116, D9Mit212) 3.9 ± 1.3 (D9Mit200) 3.0 ± 1.1 (D9Mit20) 7.8 ± 1.8 (D9Mit18). The GTP concentration determining trait appears to be a property of erythrocytes as no differences were observed for GTP/ATP ratios of brain, kidney, liver, and tongue from a low GTP strain, C3H/HeHa × Pgk-1a and a high GTP strain, C57BL/6J.Key words: Gtpc, guanosine-5'-triphosphate, GTP, mouse, chromosome 9.

scholarly journals A chromosomal segment conserved since divergence of lineages leading to man and mouse: The gene order of aminoacylase-1, transferrin, and beta-galactosidase on mouse Chromosome 9

1986 ◽  
Vol 48 (3) ◽  
pp. 175-178 ◽  
Author(s):  
Joseph H. Nadeau
Keyword(s):  
Mouse Chromosome ◽  
Gene Order ◽  
Chromosome 9 ◽  
Chromosomal Segment ◽  
Beta Galactosidase

SummaryLinkage crosses were used to determine the gene order of aminoacylase-1, beta-galactosidase, and transferrin on mouse Chromosome 9. The most likely gene order, which was more than 107 times more likely than the next most likely order, was transferrin, aminoacylase-1, beta-galactosidase. These results demonstrate that aminoacylase-1 and beta-galactosidase mark a chromosomal segment that has been conserved since divergence of lineages leading to man and mouse. The length of this segment is about 11 cM and the estimate of the true length of the conserved segment marked by these two genes is 33 cM. A second aminoacylase allozyme, which requires MgCl2, is described.

Assignment of a gene that determines erythrocytic guanosine-5′-triphosphate concentration (Gtpc) to mouse chromosome 9

Genome ◽  
1994 ◽  
Vol 37 (3) ◽  
pp. 399-404 ◽  
Author(s):  
Jack P. Jenuth ◽  
Ernest Fung ◽  
Floyd F. Snyder
Keyword(s):  
Mouse Chromosome ◽  
Strain Distribution ◽  
Recombination Frequency ◽  
Inbred Mouse ◽  
Inbred Strains ◽  
Chromosome 9 ◽  
Mouse Strains ◽  
Discrete Groups ◽  
Strain Distribution Pattern ◽  
Backcross Analysis

Nine inbred mouse strains surveyed for erythrocytic guanosine-5′-triphosphate (GTP) concentration were found to segregate into two discrete groups. Strains having low GTP levels between 1.4 and 3.4 nmol/109 cells were C3H/HeJ, C3H/HeHa, A/J, and WB/ReJ. Strains having high GTP levels between 11.0 and 14.8 nmol/109 cells were AKR/J, DBA/2J, CBA/J, C57BL/6J, and C57L/J. Erythrocytic ATP levels did not vary significantly among these groups. Crosses between low and high GTP strains gave F1 progeny having intermediate levels of GTP, and the progeny of F1's backcrossed to parental strains segregated in a 1:1 ratio for GTP concentration. We designated the GTP concentration determining trait, Gtpc. Typing the C57BL/6J × C3H/HeJ (B × H) recombinant inbred strains for GTP levels revealed 0/12 strain distribution pattern differences for loci on both chromosomes 5 and 9. Backcross analysis did not provide evidence for linkage of Gtpc to W (dominant white spotting) on chromosome 5 with 15/45 recombinants. A test for linkage of Gtpc to transferrin (Trf) on chromosome 9 gave evidence of linkage with an observed recombination frequency of 14.6 ± 5.5 and a 99% confidence interval of 3.9–33.9 cM.Key words: guanosine-5′-triphosphate, GTP, mouse, chromosome 9.

scholarly journals The Hox-2 homeo box gene complex on mouse chromosome 11 is closely linked to Re.

Genetics ◽  
1988 ◽  
Vol 118 (2) ◽  
pp. 319-327
Author(s):  
C P Hart ◽  
D K Dalton ◽  
L Nichols ◽  
L Hunihan ◽  
T H Roderick ◽  
...  
Keyword(s):  
Mouse Chromosome ◽  
Inbred Strains ◽  
Gene Complex ◽  
Chromosome 11 ◽  
Homeo Box ◽  
Length Polymorphisms ◽  
Inbred Strains Of Mice ◽  
Genetic Cross ◽  
Mouse Chromosome 11 ◽  
Restriction Fragment Length

Abstract Restriction fragment length polymorphisms have been identified between inbred strains of mice for the homeo box gene complex Hox-2. These genetic markers were used to follow the segregation of different Hox-2 alleles among recombinant inbred strains of mice and among the progeny of a three point genetic cross. The results place the Hoax-2 locus approximately 1 cM from the rex (Re) locus on mouse chromosome 11.

CONSERVED LINKAGE WITHIN A 4-cM REGION OF MOUSE CHROMOSOME 9 AND HUMAN CHROMOSOME 11

Genetics ◽  
1984 ◽  
Vol 107 (3) ◽  
pp. 463-475
Author(s):  
T K Antonucci ◽  
O H Von Deimling ◽  
B B Rosenblum ◽  
L C Skow ◽  
M H Meisler
Keyword(s):  
Human Chromosome ◽  
Inbred Strain ◽  
Mouse Chromosome ◽  
Gene Order ◽  
Chromosome Region ◽  
Chromosome 9 ◽  
Chromosome 11 ◽  
Feral Populations ◽  
Apo Ai

ABSTRACT A six-point cross was carried out to determine the gene order and distances among loci on mouse chromosome 9. Our results are consistent with the following arrangement: centromere - Lap-1 - (1.2 ± 0.8) - Es-17 - (3.0 ± 1.0) - Ups - (1.3 ± 0.7) - Alp-1 - (23.1 ± 3.4) - Mod-1 - (10.9 ± 2.6) - Acy-1. This study provides the first estimate of the distances between Es-17, Ups and Alp-1. Exceptions to the preferred association of alleles of Es-17 and Ups have been found in three feral populations and one inbred strain. Evidence is presented for the homology of this chromosome region with the ESA4 - UPS - APO-AI region on the long arm of human chromosome 11.

scholarly journals Erratum

1987 ◽  
Vol 50 (1) ◽  
pp. 77-77
Author(s):  
Joseph H. Nadeau
Keyword(s):  
Mouse Chromosome ◽  
Gene Order ◽  
Chromosome 9 ◽  
Chromosomal Segment ◽  
Beta Galactosidase

Genet. Res., Camb. (1986), 48, pp. 175–178A chromosomal segment conserved since divergence of lineages leading to man and mouse: the gene order of aminoacylase-1, transferrin and beta-galactosidase on mouse chromosome 9In the summary on page 175 and in the last paragraph on page 176, the second aminoacylase locus, Acy-2, was inadvertently described as requiring MgC12. The correct compound is MnC12.

scholarly journals LINKAGE OF GENETIC DETERMINANTS FOR MOUSE β-GALACTOSIDASE ELECTROPHORESIS AND ACTIVITY

Genetics ◽  
1977 ◽  
Vol 85 (1) ◽  
pp. 73-84
Author(s):  
Gail A M Breen ◽  
Aldons J Lusis ◽  
Kenneth Paigen
Keyword(s):  
Inbred Strains ◽  
Mammalian Genome ◽  
Chromosome 9 ◽  
Structural Genes ◽  
Genetic Determinants ◽  
Developmental Program ◽  
Inbred Strains Of Mice ◽  
Close Proximity ◽  
Mendelian Factor ◽  
Electrophoretic Polymorphism

ABSTRACT An electrophoretic polymorphism for β-galactosidase has been identified among common inbred strains of mice. It is inherited as a single Mendelian factor with two alleles showing codominant expression. This structural gene, Bge, is closely linked (0/163 recombinants) with the Bgs site on chromosome 9 which regulates systemic levels of β-galactosidase. The distribution of electrophoretic and activity phenotypes among inbred strains is not concordant, indicating that they result from separate mutations. Three aspects of β-galactosidase realization, its structure, systemic regulation and developmental program, are now known to be organized in close proximity on chromosome 9. Considered in conjunction with evidence from other mammalian systems, this suggests that the mammalian genome, like Drosophila, is organized into large functional units in which relevant regulatory and developmental information is closely associated with individual structural genes.

Effects of Foster Nursing on Alcohol Selection in Inbred Strains of Mice

1972 ◽  
Vol 33 (2) ◽  
pp. 494-503 ◽  
Author(s):  
Setsuo Komura ◽  
Masao Ueda ◽  
Toshikiyo Kobayashi
Keyword(s):  
Inbred Strains ◽  
Inbred Strains Of Mice
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