Sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.)

Genome ◽  
1999 ◽  
Vol 42 (2) ◽  
pp. 210-217 ◽  
Author(s):  
Bruno Hüttel ◽  
Peter Winter ◽  
Kurt Weising ◽  
Wafa Choumane ◽  
Franz Weigand ◽  
...  
Keyword(s):  
Cicer Arietinum ◽  
Gene Diversity ◽  
Recombinant Inbred Lines ◽  
Mendelian Inheritance ◽  
Genomic Libraries ◽  
Cicer Arietinum L ◽  
Microsatellites Dna ◽  
Pcr Products ◽  
Small Test ◽  
Site Markers

Two small-insert genomic libraries of chickpea (Cicer arietinum L.) were screened with a set of microsatellite-specific oligonucleotide probes. A total of 121 positive clones were identified among 13 000 plated colonies. Thirty-nine clones were recognized by (TAA)5, 26 by (GA)8, 18 by (GT)8, 27 by a pool of AT-rich trinucleotide repeats [(CAA)5, (CAT)5, and (GAA)5], and 11 by a pool of GC-rich trinucleotides [(TCC)5, (CAC)5, (CAG)5, and (CGA)5]. Of 53 clones selected for sequencing, 43 carried a microsatellite. Flanking primer pairs were designed for 28 loci, and used on a small test-set comprising one C. reticulatum and four C. arietinum accessions. Separation of the PCR products on agarose or polyacrylamide gels revealed single bands of the expected size with 22 of the primer pairs. Sixteen of these "Cicer arietinum sequence-tagged microsatellite site" (CaSTMS) markers were polymorphic at an intraspecific level, detecting 2-4 alleles within the four accessions examined. Primer pairs CaSTMS10 and CaSTMS15 revealed 25 and 16 alleles among 63 C. arietinum accessions from different geographic locations, reflecting gene diversity values of 0.937 and 0.922, respectively. Mendelian inheritance of CaSTMS markers was demonstrated using a set of recombinant inbred lines and their parents.Key words: chickpea, molecular markers, STMS, microsatellites, DNA polymorphism.

scholarly journals Genetic Diversity Analysis in Chickpea (Cicer arietinum L) Genotypes Grown in Northwestern Algeria using Microsatellite Markers (SSR)

2019 ◽  
Author(s):  
Bouri Amina ◽  
Mediouni Mohammed Rida ◽  
Ameur Ameur Abdelkader ◽  
Udupa Sripada ◽  
Gaouar Souheil Bechir Semir
Keyword(s):  
Genetic Diversity ◽  
Information Content ◽  
Cicer Arietinum ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Geographic Origin ◽  
Diversity Analysis ◽  
Factorial Correspondence Analysis ◽  
Genetic Diversity Analysis ◽  
Cicer Arietinum L

The present study aimed to characterize a subset of 10 selected chickpea accessions (Cicer arietinum L.) using SSR. The result indicated a presence of a total of 59 alleles. The genetic diversity at the 15 microsatellites loci was varied from 0, 32 for TA22 to 0.78 for TA72 and TA117 with an average of 0.66. Polymorphic information content (PIC) values ranged from 0.27 to 0.74. This study also detected a high significant (P less than 0.01) positive correlation between alleles per locus, gene diversity (H) and polymorphism information content (PIC). In the dendrogram and on the PCoA bi-plots, chickpea genotypes were adjoined according to their geographic origin, type of chickpea (Kabuli/ Desi). Nevertheless, the distribution of the different grouping through the factorial correspondence analysis (AFC) is due to the genetic variability.

Development of sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.)

2006 ◽  
Vol 6 (1) ◽  
pp. 93-95 ◽  
Author(s):  
SHALU CHOUDHARY ◽  
NIROJ K. SETHY ◽  
BHUMIKA SHOKEEN ◽  
SABHYATA BHATIA
Keyword(s):  
Cicer Arietinum ◽  
Cicer Arietinum L ◽  
Site Markers

DIFERENCIACIÓN DE ACCESIONES DE GARBANZO MEDIANTE CARACTERES MORFOLÓGICOS Y MOLECULARES

2018 ◽  
Vol 41 (4) ◽  
pp. 469-476
Author(s):  
Ernestina Valadez-Moctezuma ◽  
Anselmo de J. Cabrera-Hidalgo
Keyword(s):  
Cicer Arietinum ◽  
Cicer Arietinum L

El garbanzo (Cicer arietinum L.) es una de las principales leguminosas de grano cultivadas en el mundo. México es de los diez principales productores a nivel mundial con una producción total de 171 mil toneladas de grano. Este cultivo presenta alto nivel de autogamia y de monotonía genética, lo que dificulta la diferenciación de genotipos élite. En este estudio preliminar se evaluó la variabilidad de 57 genotipos de diferentes áreas geográficas mediante caracteres morfológicos y marcadores moleculares tipo RAPD e ISSR. El análisis de correspondencia múltiple mostró que los mayores valores discriminantes fueron el color de la flor azul y semillas grandes y de forma redondeada, pero el agrupamiento respectivo no diferenció a las accesiones, incluyendo las especies silvestres; sin embargo, el análisis UPGMA logró una mejor separación. Los marcadores RAPD aun cuando generaron perfiles de ADN, no fueron informativos, mientras que los ISSR diferenciaron a las 57 accesiones de C. arietinum utilizadas y a la especie silvestre C. reticulatum, lo que los hace buenos candidatos para caracterizar este cultivo. Este estudio sirvió como base para desarrollar otro sistema de marcadores moleculares más eficiente en esta especie.

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