Primed in situ labeling (PRINS): a method for rapid identification and quantification of human chromosomes in both lymphocytes and sperm nuclei

Genome ◽  
1998 ◽  
Vol 41 (5) ◽  
pp. 739-741 ◽  
Author(s):  
Antonio Musio ◽  
Paolo Baroli ◽  
Isabella Sbrana
Keyword(s):  
Satellite Dna ◽  
Human Sperm ◽  
Rapid Identification ◽  
Human Chromosomes ◽  
Chromosome X ◽  
Specific Primer ◽  
Sperm Nuclei ◽  
Identification And Quantification

In this work, a specific primer for X alphoid satellite DNA was used to detect chromosome X through primed in situ labeling (PRINS). The method allows the rapid identification of chromosome X in metaphase and its quantification in interphase. PRINS is equally applicable to both lymphocytes and sperm nuclei.Key words: PRINS, human sperm, chromosome X.

scholarly journals The impact of StuI digestion in situ on FISH to human chromosomes with satellite DNA probes

Heredity ◽  
2003 ◽  
Vol 90 (4) ◽  
pp. 298-301 ◽  
Author(s):  
M Nieddu ◽  
G Pichiri ◽  
V Melis ◽  
R Mezzanotte
Keyword(s):  
Satellite Dna ◽  
Dna Probes ◽  
Human Chromosomes ◽  
The Impact

scholarly journals Effects of Mutations in the Drosophila melanogaster Rif1 Gene on the Replication and Underreplication of Pericentromeric Heterochromatin in Salivary Gland Polytene Chromosomes

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1501
Author(s):  
Tatyana D. Kolesnikova ◽  
Alexandra V. Kolodyazhnaya ◽  
Galina V. Pokholkova ◽  
Veit Schubert ◽  
Viktoria V. Dovgan ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Satellite Dna ◽  
Polytene Chromosomes ◽  
Replication Timing ◽  
Wild Type ◽  
Chromosome X ◽  
Substantial Fraction ◽  
In The Wild ◽  
Specific Control

In Drosophila salivary gland polytene chromosomes, a substantial portion of heterochromatin is underreplicated. The combination of mutations SuURES and Su(var)3-906 results in the polytenization of a substantial fraction of unique and moderately repeated sequences but has almost no effect on satellite DNA replication. The Rap1 interacting factor 1 (Rif) protein is a conserved regulator of replication timing, and in Drosophila, it affects underreplication in polytene chromosomes. We compared the morphology of pericentromeric regions and labeling patterns of in situ hybridization of heterochromatin-specific DNA probes between wild-type salivary gland polytene chromosomes and the chromosomes of Rif1 mutants and SuUR Su(var)3-906 double mutants. We show that, despite general similarities, heterochromatin zones exist that are polytenized only in the Rif1 mutants, and that there are zones that are under specific control of Su(var)3-9. In the Rif1 mutants, we found additional polytenization of the largest blocks of satellite DNA (in particular, satellite 1.688 of chromosome X and simple satellites in chromosomes X and 4) as well as partial polytenization of chromosome Y. Data on pulsed incorporation of 5-ethynyl-2′-deoxyuridine (EdU) into polytene chromosomes indicated that in the Rif1 mutants, just as in the wild type, most of the heterochromatin becomes replicated during the late S phase. Nevertheless, a significantly increased number of heterochromatin replicons was noted. These results suggest that Rif1 regulates the activation probability of heterochromatic origins in the satellite DNA region.

Incidence of chromosome 18 disomy in human sperm nuclei as detected by nonisotopic in situ hybridization

1994 ◽  
Vol 93 (4) ◽  
Author(s):  
Martina Guttenbach ◽  
Renate Schakowski ◽  
Michael Schmid
Keyword(s):  
In Situ Hybridization ◽  
Human Sperm ◽  
Chromosome 18 ◽  
Sperm Nuclei
Sign in / Sign up

Export Citation Format

Share Document