A novel RNA species from the turtle mitochondrial genome: induction and regulation of transcription and processing under anoxic and freezing stresses

Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 534-543 ◽  
Author(s):  
Qinyin Cai ◽  
Kenneth B. Storey
Keyword(s):  
Gene Cluster ◽  
Rna Processing ◽  
Trna Gene ◽  
Trachemys Scripta ◽  
The Other ◽  
Physiological Adaptation ◽  
Regulation Of Transcription ◽  
External Stresses ◽  
Cloned Cdna ◽  
Turtle Heart

The present study identifies a previously cloned cDNA, pBTaR914, as homologous to the mitochondrial WANCY (tryptophan, alanine, asparagine, cysteine, and tyrosine) tRNA gene cluster. This cDNA clone has a 304-bp sequence and its homologue, pBTaR09, has a 158-bp sequence with a long poly(A)+ tail (more than 60 adenosines). RNA blotting analysis using pBTaR914 probe against the total RNA from the tissues of adult and hatchling turtles revealed five bands: 540, 1800, 2200, 3200, and 3900 nucleotides (nt). The 540-nt transcript is considered to be an intact mtRNA unit from a novel mtDNA gene designated WANCYHP that overlaps the WANCY tRNA gene cluster region. This transcript was highly induced by both anoxic and freezing stresses in turtle heart. The other transcripts are considered to be the processed intermediates of mtRNA transcripts with WANCYHP sequence. All these transcripts were differentially regulated by anoxia and freezing in different organs. The data suggest that mtRNA processing is sensitive to regulation by external stresses, oxygen deprivation, and freezing. Furthermore, the fact that the WANCYHP transcript is highly induced during anoxic exposure suggests that it may play an important role in the regulation of mitochondrial activities to coordinate the physiological adaptation to anoxia.Key words: mitochondria, RNA processing, anoxia, freezing, Trachemys scripta.

scholarly journals Transcription initiation and RNA processing of a yeast mitochondrial tRNA gene cluster

1987 ◽  
Vol 15 (18) ◽  
pp. 7381-7394 ◽  
Author(s):  
Rémy Borodonné ◽  
Guy Dirheimer ◽  
Robert P. Martin
Keyword(s):  
Gene Cluster ◽  
Rna Processing ◽  
Transcription Initiation ◽  
Trna Gene ◽  
Mitochondrial Trna ◽  
Mitochondrial Trna Gene

scholarly journals Characterization of a rat tRNA gene cluster containing the genes for tRNAAsp, tRNAGlyand tRNAGhi, and pseudogenes

1982 ◽  
Vol 10 (14) ◽  
pp. 4441-4448 ◽  
Author(s):  
Kyoko Shibuya ◽  
Shigeru Noguchi ◽  
Susuma Nishimura ◽  
Takao Sekiya
Keyword(s):  
Gene Cluster ◽  
Trna Gene

scholarly journals A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c

2009 ◽  
Vol 191 (21) ◽  
pp. 6612-6617 ◽  
Author(s):  
Robert M. Stagg ◽  
Swee-Seong Tang ◽  
Nils I. A. Carlin ◽  
Kaisar A. Talukder ◽  
Phung D. Cam ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Shigella Flexneri ◽  
Functional Expression ◽  
Transposon Mutagenesis ◽  
The Other ◽  
Bacterial Chromosome ◽  
Gene Encoding ◽  
O Antigen ◽  
Species Analysis

ABSTRACT The O antigen of serotype 1c differs from the unmodified O antigen of serotype Y by the addition of a disaccharide (two glucosyl groups) to the tetrasaccharide repeating unit. It was shown here that addition of the first glucosyl group is mediated by the previously characterized gtrI cluster, which is found within a cryptic prophage at the proA locus in the bacterial chromosome. Transposon mutagenesis was performed to disrupt the gene responsible for addition of the second glucosyl group, causing reversion to serotype 1a. Colony immunoblotting was used to identify the desired revertants, and subsequent sequencing, cloning, and functional expression successfully identified the gene encoding serotype 1c-specific O-antigen modification. This gene (designated gtrIC) was present as part of a three-gene cluster, similar to other S. flexneri glucosyltransferase genes. Relative to the other S. flexneri gtr clusters, the gtrIC cluster is more distantly related and appears to have arrived in S. flexneri from outside the species. Analysis of surrounding sequence suggests that the gtrIC cluster arrived via a novel bacteriophage that was subsequently rendered nonfunctional by a series of insertion events.

scholarly journals HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes

Cancers ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 4686
Author(s):  
Aida Barreiro-Alonso ◽  
Mónica Lamas-Maceiras ◽  
Lidia Lorenzo-Catoira ◽  
Mercedes Pardo ◽  
Lu Yu ◽  
...  
Keyword(s):  
Cancer Progression ◽  
Protein Interactions ◽  
Rna Processing ◽  
Ribosome Biogenesis ◽  
Affinity Purification ◽  
Regulation Of Transcription ◽  
Nurd Complex ◽  
Hmgb1 Protein ◽  
Ovary Cancer ◽  
Public Data

This study reports the HMGB1 interactomes in prostate and ovary cancer cells lines. Affinity purification coupled to mass spectrometry confirmed that the HMGB1 nuclear interactome is involved in HMGB1 known functions such as maintenance of chromatin stability and regulation of transcription, and also in not as yet reported processes such as mRNA and rRNA processing. We have identified an interaction between HMGB1 and the NuRD complex and validated this by yeast-two-hybrid, confirming that the RBBP7 subunit directly interacts with HMGB1. In addition, we describe for the first time an interaction between two HMGB1 interacting complexes, the septin and THOC complexes, as well as an interaction of these two complexes with Rab11. Analysis of Pan-Cancer Atlas public data indicated that several genes encoding HMGB1-interacting proteins identified in this study are dysregulated in tumours from patients diagnosed with ovary and prostate carcinomas. In PC-3 cells, silencing of HMGB1 leads to downregulation of the expression of key regulators of ribosome biogenesis and RNA processing, namely BOP1, RSS1, UBF1, KRR1 and LYAR. Upregulation of these genes in prostate adenocarcinomas is correlated with worse prognosis, reinforcing their functional significance in cancer progression.

scholarly journals SlyA and H-NS Regulate Transcription of the Escherichia coli K5 Capsule Gene Cluster, and Expression of slyA in Escherichia coli Is Temperature-dependent, Positively Autoregulated, and Independent of H-NS

2007 ◽  
Vol 282 (46) ◽  
pp. 33326-33335 ◽  
Author(s):  
David Corbett ◽  
Hayley J. Bennett ◽  
Hamdia Askar ◽  
Jeffrey Green ◽  
Ian S. Roberts
Keyword(s):  
Escherichia Coli ◽  
Gene Cluster ◽  
Regulation Of Transcription ◽  
Temperature Dependent ◽  
Mobility Shift ◽  
E Coli ◽  
Dnase I Footprinting ◽  
Nucleoprotein Complex ◽  
Electrophoretic Mobility Shift Assays

In this paper, we present the first evidence of a role for the transcriptional regulator SlyA in the regulation of transcription of the Escherichia coli K5 capsule gene cluster and demonstrate, using a combination of reporter gene fusions, DNase I footprinting, and electrophoretic mobility shift assays, the dependence of transcription on the functional interplay between H-NS and SlyA. Both SlyA and H-NS bind to multiple overlapping sites within the promoter in vitro, but their binding is not mutually exclusive, resulting in a remodeled nucleoprotein complex. In addition, we show that expression of the E. coli slyA gene is temperature-regulated, positively autoregulated, and independent of H-NS.

Hosts of Amblyomma dissimile Koch, 1844 and Amblyomma rotundatum Koch, 1844 (Acari: Ixodidae)

Zootaxa ◽  
2010 ◽  
Vol 2541 (1) ◽  
pp. 27 ◽  
Author(s):  
ALBERTO A. GUGLIELMONE ◽  
SANTIAGO NAVA
Keyword(s):  
Host Range ◽  
Tick Species ◽  
Wide Distribution ◽  
Trachemys Scripta ◽  
The Other ◽  
Dasypus Novemcinctus ◽  
Number Of Species ◽  
Boa Constrictor ◽  
Hydrochoerus Hydrochaeris ◽  
Natural Hosts

Host records of Amblyomma dissimile Koch, 1844 and Amblyomma rotundatum Koch, 1844 from the literature were critically reviewed. A total of 417 records on 101 species of tetrapods, and 193 records in 74 species of tetrapods were determined for A. dissimile and A. rotundatum, respectively. Aves have been found only once infested with A. dissimile. This tick has been detected on four species of Bufonidae, while A. rotundatum has been recorded on 13 species from six families of Anura. Crocodilia has been recorded infested by A. rotundatum (captive host, one species) and A. dissimile (two species). Sixteen species of Mammalia from ten families and eight species from eight families have been found infested with A. dissimile and A. rotundatum, including humans, respectively. A total of 63 species of Squamata (10 families) were found infested with A. dissimile, while the corresponding numbers for A. rotundatum are 45 species in nine families. A total of 15 species of Testudines (four families) and nine species (three families) have been found infested with A. dissimile and A. rotundatum, respectively. When infestation on captive and laboratory hosts were excluded from the analysis the number of species naturally infested with A. dissimile diminished to 88 and 58 for A. rotundatum. However, natural hosts infested with larvae, nymphs and adults of A. dissimile are Bufo marinus (Linnaeus), Bufo peltocephalus Tschudi, Proechimys semispinosus (Tomes), Boa constrictor Linnaeus, Epicrates striatus (Fischer), Oxybelis aeneus (Wagler), Cyclura cychlura (Cuvier), Iguana iguana (Linnaeus), Tupinambis teguixin (Linnaeus) and Trachemys scripta (Thunberg), but the commonest hosts harbouring all parasitic stages are B. marinus, B. constrictor and I. iguana. Hosts for all parasitic stages of A. rotundatum are B. marinus, Bufo schneideri Werner and B. constrictor, although records on B. marinus are considerably higher than the records on the other two hosts. The contribution of sheep and Hydrochoerus hydrochaeris (Linnaeus) as hosts of A. dissimile, and Dasypus novemcinctus Linnaeus as host of A. rotundatum, were overestimated in previous studies. The ample host-range of these tick species may partly explain their wide distribution from southern U.S.A. to northern Argentina, but there are also chances that more than one species are represented under the names A. dissimile and A. rotundatum.

Characterization of a Rat tRNA Gene Cluster: Comparison of Nucleotide Sequences of the Gene for tRNALeu Newly Found in Repeating Units1

1985 ◽  
Vol 97 (6) ◽  
pp. 1719-1725 ◽  
Author(s):  
Kyoko SHIBUYA ◽  
Shigeru NOGUCHI ◽  
Mineo YAMAKI ◽  
Susumu NISHIMURA ◽  
Takao SEKIYA
Keyword(s):  
Gene Cluster ◽  
Trna Gene ◽  
Nucleotide Sequences

Analysis of a drosophila tRNA gene cluster

Cell ◽  
1980 ◽  
Vol 19 (4) ◽  
pp. 889-895 ◽  
Author(s):  
Bernd Hovemann ◽  
Stephen Sharp ◽  
Hirotomo Yamada ◽  
Dieter Söll
Keyword(s):  
Gene Cluster ◽  
Trna Gene

Characterization of a human tRNA gene cluster

1990 ◽  
Vol 18 (4) ◽  
pp. 602-602 ◽  
Author(s):  
DAVID BOURN ◽  
TOM CARR ◽  
DAVID LIVINGSTONE ◽  
AILEEN McLAREN ◽  
JOHN P. GODDARD
Keyword(s):  
Gene Cluster ◽  
Trna Gene
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