scholarly journals Survey of microsatellite DNA in pine

Genome ◽  
1997 ◽  
Vol 40 (1) ◽  
pp. 9-17 ◽  
Author(s):  
C. S. Echt ◽  
P. May-Marquardt
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Genomic Library ◽  
Pinus Strobus ◽  
White Pine ◽  
Eastern White Pine ◽  
Dinucleotide Repeats ◽  
Tetranucleotide Repeats ◽  
Nucleotide Repeats ◽  
Simple Sequence

A large insert genomic library from eastern white pine (Pinus strobus) was probed for the microsatellite motifs (AC)n and (AG)n, all 10 trinucleotide motifs, and 22 of the 33 possible tetranucleotide motifs. For comparison with a species from a different subgenus, a loblolly pine (Pinus taeda) genomic library was also probed with the same set of di- and tri-nucleotide repeats and 11 of the tetranucleotide repeats. The four most abundant microsatellite motifs in both species were (AC)n, (AG)n, (AAT)n, and (ATC)n, which as a group accounted for over half the microsatellite sites investigated. The two dinucleotide repeats were the most abundant microsatellite motifs tested in both species, each at 2–4.5 sites/megabase pair (Mbp), but the two trinucleotide motifs were nearly as abundant and are considered good candidates for pine microsatellite marker development efforts. Eastern white pine had more than twice as many (AC)n as (AG)n loci, in contrast with loblolly pine and most other plant species in which (AG)n is more abundant. In both pine species the minimum estimated genome density for all microsatellites, excluding (AT)n repeats, was 16 sites/Mbp.Key words: Pinus strobus, Pinus taeda, eastern white pine, loblolly pine, simple sequence repeats.

scholarly journals Developing Microsatellite DNA Markers in Pecan

2003 ◽  
Vol 128 (3) ◽  
pp. 374-380 ◽  
Author(s):  
L.J. Grauke ◽  
Muhammad J. Iqbal ◽  
Avutu S. Reddy ◽  
Tommy E. Thompson
Keyword(s):  
Dna Markers ◽  
Microsatellite Dna ◽  
Southern Hybridization ◽  
Genomic Library ◽  
Carya Illinoinensis ◽  
Enriched Library ◽  
Microsatellite Dna Markers ◽  
Ssr Primers ◽  
Nucleotide Repeats ◽  
Simple Sequence

A microsatellite-enriched library was developed from `Halbert', a native pecan [Carya illinoinensis (Wangenh.) K. Koch] selection from Coleman County, Texas. A genomic library enriched for simple sequence repeats (SSR) containing 6144 clones was archived in 384 well plates for screening. In total, 439 clones were identified after Southern hybridization using di- and tri-nucleotide repeats as probes. In total, 125 positive clones were sequenced and primers were designed for 24 repeats. The SSR markers chosen for analysis include di-(CT and GA) and tri-nucleotide repeats (CTT, GAA and GAT). Of the 24 primer pairs tested, 19 successfully amplified microsatellites from `Halbert'. DNA was isolated from 48 pecan and hickory accessions selected to strategically represent the genetic diversity of the National Clonal Germplasm Repository (NCGR) Carya collections. The 19 SSR primers that produced good amplification products in `Halbert' were used to evaluate the collection, with 11 revealing polymorphism. The number of fragments amplified with different primer combinations ranged from 4 to 32 in the 48 genotypes tested. Evaluation of the data confirms the utility of the microsatellites in delimiting known relationships.

scholarly journals AIR-LAYERING OF EASTERN WHITE PINE (Pinus strobus L.)

1959 ◽  
Vol 35 (1) ◽  
pp. 19-21 ◽  
Author(s):  
J. L. Farrar ◽  
W. D. McJannet
Keyword(s):  
Pinus Strobus ◽  
White Pine ◽  
Eastern White Pine

scholarly journals Dendrochronological Analyses and Whole-Tree Dissections Reveal Caliciopsis Canker (Caliciopsis pinea) Damage Associated with the Declining Growth and Climatic Stressors of Eastern White Pine (Pinus strobus)

Forests ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 347
Author(s):  
Kara K.L. Costanza ◽  
William H. Livingston ◽  
Shawn Fraver ◽  
Isabel A. Munck
Keyword(s):  
New England ◽  
Tree Growth ◽  
Economic Value ◽  
Pinus Strobus ◽  
White Pine ◽  
Eastern White Pine ◽  
Management Options ◽  
Biological Impacts ◽  
Insight Into ◽  
Whole Tree

Eastern white pine (Pinus strobus) is considered a signature species in eastern North America, particularly in New England. In recent years, however, white pine has experienced increased damage due to native pathogens that reduce the species’ growth, productivity, and economic value. One disease of concern is Caliciopsis canker, caused by the fungal pathogen Caliciopsis pinea, which is associated with excessive resin production, cankers, rough bark, bark fissures/cracks, and reduced growth in white pine. Recent studies have documented the extent of Caliciopsis canker in New England and its association with soil and stocking conditions, yet few studies have focused on the biological impacts of the disease. This study used dendrochronology and whole-tree dissections to reconstruct Caliciopsis canker history in three New England white pine sites, quantify its impact on tree growth and vigor, identify pre-disposing factors, and assess potential silvicultural management options. Dendrochronology and whole-tree dissections provided a unique insight into canker damage throughout trees’ development. Canker damage was first reported in New Hampshire in the mid-1990s, yet cankers were present as far back as 1967 and have steadily increased since the mid-1980s. Increased canker damage was significantly associated with decreased live crown ratios and declining tree growth. Trees maintaining a 30% live crown ratio or greater generally experienced the least canker damage. Furthermore, peaks in canker occurrence were consistent across sites, indicating a regional synchronization of infection and damage. Canker damage was closely associated with climatic events such as droughts and a New England hurricane. The results suggest that Caliciopsis canker has been affecting white pine health over the last 40 years, and that the disease has become more prevalent in the past 20–30 years. Yet, our results suggest that if silvicultural prescriptions target low density thinnings that favor trees with higher live crown ratios (>30%) and low Caliciopsis symptom severity ratings, the risk of canker damage can be reduced in white pine stands.

Winter Leaf Conductance Rates of Pinus Strobus L. (Eastern White Pine)

2008 ◽  
Vol 29 (5) ◽  
pp. 474-482
Author(s):  
Delphis F. Levia ◽  
Lydia A. Leclair
Keyword(s):  
Pinus Strobus ◽  
Leaf Conductance ◽  
White Pine ◽  
Eastern White Pine

Breeding eastern white pine for blister rust resistance: A review of progress in Ontario

2009 ◽  
Vol 85 (5) ◽  
pp. 745-755 ◽  
Author(s):  
Pengxin Lu ◽  
Darren Derbowka
Keyword(s):  
Rust Resistance ◽  
Genetic Resistance ◽  
Pinus Strobus ◽  
White Pine Blister Rust ◽  
White Pine ◽  
Poor Survival ◽  
Eastern White Pine ◽  
Blister Rust ◽  
Survival And Growth ◽  
Major Resistance Genes

White pine blister rust (caused by Cronartium ribicola J.C. Fisch.) is a primary cause of poor survival and growth of eastern white pine (Pinus strobus L.) and has contributed to the species’ decline across its range. Genetic resistance to C. ribicola is generally weak in eastern white pine, possibly owing to the absence of meaningful resistance alleles. Integrating major resistance genes from Eurasian 5-needle pine species to P. strobus has been demonstrated to be effective through traditional plant breeding approaches. This paper provides a review of progress after more than half a century of breeding and testing in Ontario. Key words: Pinus strobus, white pine blister rust, genetic resistance, interspecific hybridization

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