A physical map of the Thinopyrum-derived Lr19 translocation

Genome ◽  
1996 ◽  
Vol 39 (5) ◽  
pp. 1013-1019 ◽  
Author(s):  
R. Prins ◽  
G. F. Marais ◽  
A. S. Marais ◽  
B. J. H. Janse ◽  
Z. A. Pretorius
Keyword(s):  
Key Words ◽  
Leaf Rust ◽  
Rust Resistance ◽  
Physical Map ◽  
Leaf Rust Resistance ◽  
Chromosome Segment ◽  
Deletion Mapping ◽  
Chromosome Arm ◽  
Marker Loci

Twenty-nine lines with deletions in the Lr19 ('Indis') translocated chromosome segment were used to physically map three Thinopyrum RFLP loci as well as the Sr25 and Sd1 loci. From the data, the relative locations of marker loci on the translocation were determined as: Sd1, Xpsr165, Xpsr105, Xps129, Lr19, Wsp-D1, Sr25/Y. The data confirmed the reported homoeology between the Lr19 segment and chromosome arm 7DL of wheat. Also, it seems that the Lr19 translocation in 'Indis' is very similar to the Lr19 segment in the T4 source and that the former may not derive from Thinopyrum distichum. Key words : deletion mapping, leaf rust resistance.

Gamma irradiation induced deletions in an alien chromosome segment of the wheat 'Indis' and their use in gene mapping

Genome ◽  
1992 ◽  
Vol 35 (2) ◽  
pp. 225-229 ◽  
Author(s):  
G. F. Marais
Keyword(s):  
Leaf Rust ◽  
Stem Rust ◽  
Rust Resistance ◽  
D1 Protein ◽  
Leaf Rust Resistance ◽  
Chromosome Segment ◽  
Translocation Line ◽  
Deletion Mapping ◽  
Stem Rust Resistance Gene ◽  
Interspecific Gene Transfer

Deletion mutants were produced in a translocated chromosome segment derived from Thinopyrum distichum (Thunb.) Löve. Spikes of the translocation line 'Indis' were irradiated with gamma rays at dosages of 15, 20, and 25 Gy. The irradiated spikes were pollinated with 'Inia 66' pollen and the F2 and F3 generations screened for translocation mutants, using the genes for leaf rust resistance and yellow endosperm pigmentation as markers. Finally, endopeptidase polymorphisms were utilized to select mutant translocation homozygotes within each of 29 families. An investigation of polymorphisms at the α-Amy-D2 and Wsp-D1 loci of chromosome arm 7DL revealed that 'Indis' did not produce an α-AMY-D2 product, but it did produce a novel WSP-D1 protein. The mutants were characterized for their leaf and stem rust resistances and the presence of WSP-D1 and yellow flour pigments. The stem rust resistance gene could not be accurately mapped. The linear order of the remaining loci on 7DL was centromere - leaf rust resistance - Wsp-D1 and yellow pigment. The data obtained suggested that the 'Indis' translocation has homo(eo)logy to the Lr19 translocation and homoeology to 7DL of common wheat.Key words: wheat, interspecific gene transfer, translocation, deletion mapping.

Characterization of an incomplete leaf rust resistance gene on chromosome 1RS and development of KASP markers for Lr47 in wheat

2020 ◽  
Author(s):  
Xiangyang Xu ◽  
Genqiao Li ◽  
Guihua Bai ◽  
Amy Bernardo ◽  
Brett F Carver ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Great Plains ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene ◽  
Breeding Lines ◽  
Chromosome Arm ◽  
Kasp Markers

Leaf rust, caused by Puccininia triticina (Pt), is one of the most common wheat diseases in the Great Plains of the USA. A population of recombinant inbred lines (RILs) from CI 17884 x Bainong 418 was evaluated for responses to leaf rust race Pt52-2 and genotyped using single nucleotide polymorphism (SNP) markers. Quantitative trait locus (QTL) analysis identified a minor gene for resistance to leaf rust, designated QLr.stars-1RS, on the 1BL.1RS translocation segment in Bainong 418, and another leaf rust resistance gene, Lr47, on chromosome 7A of CI 17884. Lr47, originally identified in CI 17884 and located in a wheat-T. speltoides translocation segment 7S#1S, remains one of only a few race-specific resistance genes still effective in the Great Plains. A set of 7A-specific simple sequence repeat (SSR) markers were developed and used to genotype CI 17884 and a pair of near-isogenic lines differing in the presence or absence of 7S#1S, PI 603918 and Pavon F76. Haplotype analysis indicated that the estimated length of 7S#1S was 157.23 to 174.42 Mb, accounting for about 23% of the 7A chromosome. Two SNPs on 7S#1S and 4 SNPs on the 1RS chromosome arm were converted to KASP markers, which were subsequently validated in a panel of cultivars and recently released elite breeding lines. Of these, one and two KASP markers are specific to the 1RS chromosome arm and 7S#1S, respectively, indicating that they can facilitate the introgression of Lr47 and QLr.stars-1BS into locally adapted wheat cultivars and breeding lines.

A novel leaf rust resistance gene introgressed from Aegilops markgrafii maps on chromosome arm 2AS of wheat

2020 ◽  
Vol 133 (9) ◽  
pp. 2685-2694 ◽  
Author(s):  
K. Rani ◽  
B. R. Raghu ◽  
S. K. Jha ◽  
Priyanka Agarwal ◽  
Niharika Mallick ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene ◽  
Chromosome Arm

Linkage relations of Gli-D1, Rg2, and Lr21 on the short arm of chromosome 1D in wheat

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 937-940 ◽  
Author(s):  
S. S. Jones ◽  
J. Dvořák ◽  
C. O. Qualset
Keyword(s):  
Triticum Aestivum ◽  
Leaf Rust ◽  
Storage Protein ◽  
Chinese Spring ◽  
Rust Resistance ◽  
Storage Proteins ◽  
Seed Storage Protein ◽  
Leaf Rust Resistance ◽  
Triticum Tauschii ◽  
Chromosome Arm

Homozygous recombinant substitution lines, derived from a cross of Triticum aestivum 'Chinese Spring' with a disomic substitution line of Triticum tauschii chromosome 1D in 'Chinese Spring', were used to investigate the linkage relationships among the loci Glu-D1, encoding high molecular weight glutenin storage proteins, Gli-D1, encoding gliadin storage proteins, Rg2, controlling glume color, and Lr21, conferring leaf-rust resistance. Gli-D1, on chromosome arm 1DS, is tightly linked to Rg2 and Lr21 (1.4 and 5.6% recombination, respectively). The order of the loci is Gli-D1–Rg2–Lr21. Glu-D1, on chromosome arm 1DL, segregates independently (P = 0.43) of Gli-D1, Rg2, and Lr21. The position of Glu-D1, Gli-D1, Rg2, and Lr21 in the genetic linkage map of chromosome 1D agrees with the position of storage protein, glume color, and rust-resistance loci on chromosomes 1A and 1B.Key words: leaf-rust resistance, seed storage protein, glutenin, gliadin, glume color, Triticum aestivum, Triticum tauschii.

scholarly journals The recognition of an alien chromosome segment translocated to a wheat chromosome

1967 ◽  
Vol 10 (3) ◽  
pp. 295-301 ◽  
Author(s):  
G. Kimber
Keyword(s):  
Leaf Rust ◽  
Rust Resistance ◽  
Wheat Variety ◽  
Wheat Chromosome ◽  
Leaf Rust Resistance ◽  
Chromosome Segment ◽  
Interference Microscopy ◽  
Alien Chromosome ◽  
Small Segment

1. Chromosome 6B of T. aestivum, to which a small segment of an Ae. umbellulata chromosome had been translocated, was examined both by interference microscopy and in stained preparations.2. In the stained preparation a constriction was observed in the long arm of chromosome 6B and also a terminal knob that was also observed on chromosome A of Ae. umbellulata.3. These features were also observed by interference microscopy and in addition the portion of the chromosome 6B of Transfer distal to the constriction had different phase-retarding properties.4. It is concluded that the part of chromosome 6B distal to the constriction is the part of Ae. umbellulata chromosome A which carries the gene for the leaf-rust resistance of that species and of the wheat variety into which it was translocated.

scholarly journals Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Vishal Dinkar ◽  
S. K. Jha ◽  
Niharika Mallick ◽  
M. Niranjana ◽  
Priyanka Agarwal ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Ssr Markers ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene ◽  
Chromosome Arm ◽  
The Cross

AbstractTSD276-2, a wheat genetic stock derived from the cross Agra Local/T. spelta 276 showed broad spectrum resistance against leaf rust pathogen. Genetic analysis was undertaken using F1, F2, F2:3 and BC1F1 generations derived from the cross TSD276-2/Agra Local. The results revealed a single recessive gene for leaf rust resistance, tentatively named as LrTs276-2, in TSD276-2. Molecular mapping of leaf rust resistance gene LrTs276-2 in TSD276-2 was done using SNP-based PCR and SSR markers. For Bulked Segregant Analysis (BSA), two bulks viz. resistant bulk and susceptible bulk, and the parents TSD276-2 and Agra Local were genotyped for SNPs using AFFYMETRIX 35K Wheat Breeders' AXIOM array. T. spelta 276 was also genotyped and used as a check. BSA indicated that the gene for leaf rust resistance in TSD276-2 is located on chromosome arm 1DS. Putatively linked SNPs on chromosome arm 1DS were converted into PCR-based markers. Polymorphic SSR markers on chromosome arm 1DS were also identified. Final linkage map was constructed using one SNP-based PCR and three SSR markers. The rust reaction and chromosomal location suggest that LrTs276-2 is a new leaf rust resistance gene which may be useful in broadening the genetic base of leaf rust resistance in wheat.

TELOCENTRIC MAPPING IN HEXAPLOID WHEAT OF GENES FOR LEAF RUST RESISTANCE AND OTHER CHARACTERS DERIVED FROM AEGILOPS SQUARROSA

1974 ◽  
Vol 16 (1) ◽  
pp. 137-144 ◽  
Author(s):  
G. G. Rowland ◽  
E. R. Kerber
Keyword(s):  
Leaf Rust ◽  
Hexaploid Wheat ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Synthetic Hexaploid Wheat ◽  
Adult Plant ◽  
Aegilops Squarrosa ◽  
Chromosome Arm ◽  
Synthetic Hexaploid ◽  
Telocentric Mapping

Telocentrics of hexaploid wheat, Triticum aestivum spp. vulgare cv. Chinese Spring, were used to establish the chromosome arm location and crossover distance from the centromere of genes controlling characters introduced into synthetic hexaploid wheat (2n = 42 = AABBDD) from Aegilops squarrosa (2n = 14 = DD). The chromosome arm location and the crossover distance from the centromere of each gene studied are as follows: synthetic hexaploid RL 5404 — brown glumes (Rg2), 1DL, 13.3 ± 3.3%; tenacious glumes (Tg), 2Dα, 39.4 ± 4.9%; inhibitor of waxy foliage (W21), 2Dα, 52.5 ± 5.0%; adult-plant leaf rust resistance (Lr22), 2Dα, 63.6 ± 4.8%; purple coleoptile (Rc3), 7DS, 10.3 ± 2.8%; synthetic hexaploid RL 5406 — Rg2, 1DL, 1.7 ± 1.0%; Tg, 2Dα, 42.9 ± 4.6%; W21, 2Dα, 58.9 ± 4.6%; Rc3, 7DS, 9.8 ± 2.8%. A gene for seedling leaf rust resistance (Lr21) found in RL 5406 is located on chromosome 1D.

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