Identification of RAPD, SCAR, and RFLP markers tightly linked to nematode resistance genes introgressed from Arachis cardenasii into Arachis hypogaea

Genome ◽  
1996 ◽  
Vol 39 (5) ◽  
pp. 836-845 ◽  
Author(s):  
G. M. Garcia ◽  
H. T. Stalker ◽  
E. Shroeder ◽  
G. Kochert
Keyword(s):  
Arachis Hypogaea ◽  
Resistance Genes ◽  
Bulked Segregant Analysis ◽  
Rapd Marker ◽  
Dominant Gene ◽  
Nematode Resistance ◽  
Rflp Markers ◽  
Interspecific Crosses ◽  
Meloidogyne Arenaria ◽  
Root Knot Nematode

Two dominant genes conditioning resistance to the root-knot nematode Meloidogyne arenaria were identified in a segregating F2 population derived from the cross of 4x (Arachis hypogaea × Arachis cardenasii)-GA 6 and PI 261942. Mae is proposed as the designation for the dominant gene restricting egg number and Mag is proposed as the designation for the dominant gene restricting galling. The high levels of resistance in GA 6 were introgressed from A. cardenasii and, therefore, a search to identify A. cardenasii specific RAPD markers that are tightly linked to these resistance genes was conducted utilizing bulked segregant analysis. One RAPD marker (Z3/265) was linked at 10 ± 2.5 (SE) and 14 ± 2.9 cM from Mag and Mae, respectively. The marker was mapped to linkage group 1 at 5 cM from Xuga.cr239 in the backcross map in an area where introgression from A. cardenasii had previously been reported. This fragment was cloned and used to generate a pair of primers that specifically amplified this locus (sequence characterized amplified region, SCAR) and as a RFLP probe. Their close linkage with the resistance genes will be useful in marker-based selection while transferring nematode resistance from introgression lines into elite breeding lines and cultivars. The Z3/265 marker associated with the genes Mae or Mag was not found in other highly resistant Arachis species (Arachis batizocoi or Arachis stenosperma), in progenies of interspecific crosses with A. cardenasii that were moderately resistant, or in the resistant A. hypogaea lines PI 259634 and PI 259572. These represent the first molecular markers linked with a resistant gene in peanut and the first report of two physiological responses to nematode attack associated with two genetic factors. Key words : peanut, Arachis hypogaea, Arachis cardenasii, Meloidogyne arenaria, RFLP, RAPD, SCAR, nematode resistance, bulk segregant analysis, introgression.

Timing of Aldicarb Applications to Control Meloidogyne arenaria in Peanut1

2001 ◽  
Vol 28 (2) ◽  
pp. 73-75 ◽  
Author(s):  
J. R. Rich ◽  
D. W. Gorbet
Keyword(s):  
Arachis Hypogaea ◽  
Chemical Treatment ◽  
Optimal Time ◽  
Meloidogyne Arenaria ◽  
Root Knot Nematode ◽  
Population Densities ◽  
Time Intervals ◽  
Yield Increase ◽  
Post Harvest ◽  
Chemical Treatments

Abstract Four fieldtrialswere conductedin northwest Florida to determine the efficacyofaldicarb appliedat varyingtime intervals after planting on peanut (Arachis hypogaea) to manage the peanut root-knot nematode, Meloidogyne arenaria. Initial treatments with aldicarb (Temik 15G), fenamiphos (Nemacur 15G), and phorate (Thimet 15G) were made at planting of peanut cv. Southern Runner. The chemicals were applied as 20-cm-wide bands over the open seed furrow using a tractor-mounted Gandy applicator. Post-plant treatments were made with a Gandy applicator at time intervals from 28 to 104 dafter planting as 36-cm-wide bands over the row centers. Post-harvest M. arenaria population densities were affected little by any chemical treatment compared to the control. The efficacy of the chemical treatments was variable and averaged onlya 295-kglha yield increase for the single at-plant applications of aldicarb compared to the control. Allat-plant + post-plant aldicarb treatments increased yield over the control by an average of712 kg¡ ha. Results from these trials did not establish a single optimal time for post-plant application of aldicarb on peanut. Data from these tests, however, indicated that a post-plant aldicarb treatment can be applied latter than previously recommended in Florida.

scholarly journals Introgression of a Tombusvirus Resistance Locus from Nicotiana edwardsonii var. Columbia to N. clevelandii

2006 ◽  
Vol 96 (5) ◽  
pp. 453-459 ◽  
Author(s):  
James E. Schoelz ◽  
B. Elizabeth Wiggins ◽  
William M. Wintermantel ◽  
Kathleen Ross
Keyword(s):  
Mosaic Virus ◽  
Resistance Genes ◽  
Virus Resistance ◽  
Dominant Gene ◽  
Cauliflower Mosaic Virus ◽  
Ringspot Virus ◽  
N Gene ◽  
Interspecific Crosses ◽  
Addition Line ◽  
Resistance Locus

A new variety of Nicotiana, N. edwardsonii var. Columbia, was evaluated for its capacity to serve as a new source for virus resistance genes. Columbia was developed from a hybridization between N. glutinosa and N. clevelandii, the same parents used for the formation of the original N. edwardsonii. However, in contrast to the original N. edwardsonii, crosses between Columbia and either of its parents are fertile. Thus, the inheritance of virus resistance genes present in N. glutinosa could be characterized by using Columbia as a bridge plant in crosses with the susceptible parent, N. clevelandii. To determine how virus resistance genes would segregate in interspecific crosses between Columbia and N. clevelandii, we followed the fate of the N gene, a single dominant gene that specifies resistance to Tobacco mosaic virus (TMV). Our genetic evidence indicated that the entire chromosome containing the N gene was introgressed into N. clevelandii to create an addition line, designated N. clevelandii line 19. Although line 19 was homozygous for resistance to TMV, it remained susceptible to Tomato bushy stunt virus (TBSV) and Cauliflower mosaic virus (CaMV) strain W260, indicating that resistance to these viruses must reside on other N. glutinosa chromosomes. We also developed a second addition line, N. clevelandii line 36, which was homozygous for resistance to TBSV. Line 36 was susceptible to TMV and CaMV strain W260, but was resistant to other tombusviruses, including Cucumber necrosis virus, Cymbidium ringspot virus, Lettuce necrotic stunt virus, and Carnation Italian ringspot virus.

scholarly journals Evidence for a Second RKN Resistance Gene in Peanut

2016 ◽  
Vol 43 (1) ◽  
pp. 49-51 ◽  
Author(s):  
W.D. Branch ◽  
T.B. Brenneman ◽  
J.P. Noe
Keyword(s):  
Genetic Model ◽  
Dominant Gene ◽  
Recessive Gene ◽  
Nematode Resistance ◽  
Root Knot Nematode ◽  
Vicia Villosa ◽  
Arachis Hypogaea L ◽  
Race 1 ◽  
Significant Yield ◽  
Very High

ABSTRACT Root-knot nematode (RKN), [Meloidogyne arenaria (Neal) Chitwood race 1] can result in highly significant yield losses in peanut (Arachis hypogaea L.) production. Fortunately, very high levels of RKN nematode resistance have been identified and incorporated from wild species into newly developed peanut cultivars. In 2011-12 at Tifton, GA, a field site was artificially inoculated with M. arenaria race 1. A susceptible cultivar was used to uniformly increase the peanut-specific race 1 nematode population during the summer and fall; whereas, hairy vetch (Vicia villosa Roth) was used for the same purpose each winter as a susceptible cover crop. During 2013 and 2014, space-planted F2 and F3 populations from cross combinations involving A. hypogaea susceptible × resistant parental lines derived from ‘COAN’ were evaluated, respectively. Several past inheritance studies had suggested a single dominant gene, Rma, controlled the resistance. However in this study, the occurrence of a second recessive gene (rma2) was also found to be involved in very high peanut RKN resistance. Inheritance data fit a 13:3 genetic model and confirmed an earlier report for two RKN-resistance genes (Rma1 and rma2) found in TxAG-6 and now COAN.

RFLP markers linked to the root knot nematode resistance gene Mi in tomato

1991 ◽  
Vol 81 (5) ◽  
pp. 661-667 ◽  
Author(s):  
R. Klein-Lankhorst ◽  
P. Rietveld ◽  
B. Machiels ◽  
R. Verkerk ◽  
R. Weide ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Nematode Resistance ◽  
Rflp Markers ◽  
Root Knot Nematode ◽  
Nematode Resistance Gene

scholarly journals A High-Resolution Linkage Map of the Citrus Tristeza Virus Resistance Gene Region in Poncirus trifoliata (L.) Raf.

Genetics ◽  
1998 ◽  
Vol 150 (2) ◽  
pp. 883-890
Author(s):  
D Q Fang ◽  
C T Federici ◽  
M L Roose
Keyword(s):  
Resistance Gene ◽  
Plant Disease Resistance ◽  
Citrus Tristeza Virus ◽  
Bulked Segregant Analysis ◽  
Dominant Gene ◽  
Artificial Chromosome ◽  
Poncirus Trifoliata ◽  
Rflp Markers ◽  
Tristeza Virus ◽  
Citrus Tristeza

Abstract Resistance to citrus tristeza virus (CTV) was evaluated in 554 progeny of 10 populations derived from Poncirus trifoliata. A dominant gene (Ctv) controlled CTV resistance in P. trifoliata. Twenty-one dominant PCR-based DNA markers were identified as linked to Ctv by bulked segregant analysis. Of the 11 closest markers to Ctv, only 2 segregated in all populations. Ten of these markers were cloned and sequenced, and codominant RFLP markers were developed. Seven RFLP markers were then evaluated in 10 populations. Marker orders were consistent in all linkage maps based on data of single populations or on combined data of populations with similar segregation patterns. In a consensus map, the six closest marker loci spanned 5.3 cM of the Ctv region. Z16 cosegregated with Ctv. C19 and AD08 flanked Ctv at distances of 0.5 and 0.8 cM, respectively. These 3 markers were present as single copies in the Poncirus genome, and could be used directly for bacterial artificial chromosome library screening to initiate a walk toward Ctv. BLAST searches of the GenBank database revealed high sequence similarities between 2 markers and known plant disease resistance genes, indicating that a resistance gene cluster exists in the Ctv region in P. trifoliata.

Greenhouse and field resistance in cucumber to root-knot nematodes

Nematology ◽  
1999 ◽  
Vol 1 (3) ◽  
pp. 279-284 ◽  
Author(s):  
S. Alan Walters ◽  
Todd C. Wehner ◽  
Kenneth R. Barker
Keyword(s):  
Cucumis Sativus ◽  
Multiple Root ◽  
Nematode Species ◽  
Nematode Resistance ◽  
Field Resistance ◽  
Field Conditions ◽  
Meloidogyne Arenaria ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Race 2

Abstract Ten cultigens were evaluated for resistance to Meloidogyne arenaria races 1 and 2, and M. javanica under greenhouse and field conditions. Resistance to M. arenaria races 1 and 2, and M. javanica was verified in Cucumis sativus var. hardwickii line LJ 90430 and to M. arenaria race 2 in C. sativus var. sativus Southern Pickler and Mincu in a greenhouse test. Another cultigen of C. sativus var. hardwickii (PI 215589) was found to be resistant to M. arenaria race 2 but not to other root-knot nematode species tested. LJ 90430 is the cultigen of choice to develop root-knot nematode resistant cucumbers, since it has multiple root-knot nematode resistance and is cross-compatible with cucumber. Greenhouse and field data were positively correlated (r = 0.74) over both years. Experiment repeatabilities were calculated from the cultigens infected with root-knot nematodes under both greenhouse and field conditions. Four environments (greenhouse and field over 2 years) were used in the analysis. Repeatabilities were high in all instances (ranging from 0.83-0.99) and indicated that the environment (field or greenhouse) was not an important factor in assessing root-knot nematode resistance for the cultigens evaluated. Resistenz von Gurkengegen Wurzelgallennematoden im Gewachshaus undim Freiland - Unter Gewachshausund Freilandbedingungen wurden zehn Cultigene auf ihre Resistenz gegen Meloidogyne arenaria Rassen 1 und 2 und gegen M. javanica gepruft. Bei Cucumis sativus var. hardwickii Linie LJ 90430 wurde im Gewachshausversuch Resistenz gegen M. arenaria Rassen 1 und 2 sowie gegen M. javanica nachgewiesen, und in C. sativus var. sativus "Southern Pickler" und "Mincu" Resistenz gegen M. arenaria Rasse 2. Cultigen C. sativus var. hardwickii (PI 215589) war resistent gegen M. arenaria Rasse 2 aber nicht gegen die anderen gepruften Arten von Wurzelgallennematoden. LJ 90430 ist das Cultigen der Wahl bei der Entwicklung von Gurken, die gegen Wurzelgallennematoden resistent sind, da es multiple Resistenzen gegen Wurzelgallennematoden besitzt und kreuzungsvertraglich mit Gurke ist. Die Ergebnisse der Gewachshaus- und Feldversuche waren uber beide Versuchsjahre hin positiv korreliert (r = 0,74). Ausgehend von den Cultigenen, die im Gewachshaus und im Freiland mit Wurzelgallennematoden infiziert waren, wurden die Wiederholbarkeiten der Versuche berechnet. Dabei wurden vier verschiedene Umweltbedingungen (Gewachshaus und Freiland uber zwei Jahre) verwendet. Die Wiederholbarkeiten waren in allen Fallen hoch (0,83-0,99) und zeigten an, dass die Umwelt (Freiland oder Gewachshaus) kein wichtiger Faktor bei der Bestimmung der Resistenz gegen Wurzelgallennematoden bei den gepruften Cultigenen war.

New data on the specificity of the root-knot nematode resistance genes Me1 and Me3 in pepper

2001 ◽  
Vol 120 (5) ◽  
pp. 429-433 ◽  
Author(s):  
P. Castagnone-Sereno ◽  
M. Bongiovanni ◽  
C. Djian-Caporalino
Keyword(s):  
Resistance Genes ◽  
Nematode Resistance ◽  
Root Knot Nematode

Location of independent root-knot nematode resistance genes in plum and peach

2003 ◽  
Vol 108 (4) ◽  
pp. 765-773 ◽  
Author(s):  
M. Claverie ◽  
N. Bosselut ◽  
A. C. Lecouls ◽  
R. Voisin ◽  
B. Lafargue ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Nematode Resistance ◽  
Root Knot Nematode
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