Generation of expressed sequence tags and sequence-tagged sites as physical landmarks in the mosquito, Aedes aegypti, genome

Genome ◽  
1996 ◽  
Vol 39 (1) ◽  
pp. 224-229 ◽  
Author(s):  
D. W. Severson ◽  
Y. Zhang
Keyword(s):  
Aedes Aegypti ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Expressed Sequence Tags ◽  
Rapid Screening ◽  
Amino Acid Sequence Similarity ◽  
Sequence Tagged Sites ◽  
Aegypti Genome ◽  
Expressed Sequence ◽  
Restriction Fragment Length

Twenty-three clones were developed as expressed sequence tags (ESTs) and sequence-tagged sites (STSs) that provide broad coverage of the mosquito, Aedes aegypti, genome at an average spacing of about 7.2 cM. Each of these clones had been mapped previously as a restriction fragment length polymorphism (RFLP) marker. Nineteen of these clones represent anonymous cDNAs and 4 clones represent known genes. Forty-seven percent of the anonymous cDNAs showed significant deduced amino acid sequence similarity to previously described sequences from a wide variety of organisms. STSs developed from RFLPs will provide effective tools for rapid screening of YAC and cosmid libraries, and will therefore, provide anchor points for contig construction for any region in the A. aegypti genome. Also, they facilitate the simultaneous integration of physical and genetic mapping data for A. aegypti. Key words : STS, EST, RFLP, restriction fragment length polymorphisms, QTL, quantitative trait loci.

scholarly journals Sequences of Probes Revealing Mapped Restriction Fragment-length Polymorphisms in Cucumber

HortScience ◽  
2005 ◽  
Vol 40 (2) ◽  
pp. 323-324
Author(s):  
Michael J. Havey
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Expressed Sequence Tags ◽  
Restriction Fragment Length Polymorphisms ◽  
Cdna Clones ◽  
Chromosome 4 ◽  
Sequence Comparisons ◽  
Length Polymorphisms ◽  
Expressed Sequence ◽  
Restriction Fragment Length

PstI-genomic and cDNA clones revealing mapped restriction fragment-length polymorphisms (RFLP) in cucumber (Cucumis sativus L.) were sequenced in order to ensure that these clones remain available and to determine if any clones showing genetic linkage in cucumber are physically linked in Arabidopsis thaliana. Sequence comparisons using translated searches revealed that 80% of the cucumber cDNA clones showed significant (≤e-20) similarities to Arabidopsis expressed sequence tags (ESTs) or genomic sequences, as opposed to relatively few (32%) of the cucumber genomic clones. Two clones revealing RFLPs linked at 2 cM in cucumber showed significant (≤e-20) similarities to sequences separated by 347,616 basepairs on chromosome 4 of Arabidopsis.

scholarly journals Use of PCR-Restriction Fragment Length Polymorphism of inlA for Rapid Screening of Listeria monocytogenes Strains Deficient in the Ability To Invade Caco-2 Cells

2004 ◽  
Vol 70 (4) ◽  
pp. 2180-2185 ◽  
Author(s):  
S. Rousseaux ◽  
M. Olier ◽  
J. P. Lemaître ◽  
P. Piveteau ◽  
J. Guzzo
Keyword(s):  
Listeria Monocytogenes ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Rapid Screening ◽  
Pcr Rflp ◽  
Reference Strains ◽  
Restriction Fragment Length

ABSTRACT A PCR-restriction fragment length polymorphism (RFLP) method was developed in order to screen a large number of strains for impaired adhesion to epithelial cells due to expression of truncated InlA. inlA polymorphism was analyzed by PCR-RFLP in order to correlate inlA PCR-RFLP profiles and production of truncated InlA. Thirty-seven Listeria monocytogenes strains isolated from various sources, including five noninvasive and two invasive reference strains, were screened. Two endonucleases (AluI and Tsp509I) were used, and they generated five composite profiles. Thirteen L. monocytogenes isolates were characterized by two specific PCR-RFLP profiles similar to PCR-RFLP profiles of noninvasive reference strains previously described as strains that produce truncated InlA. Ten of the 13 isolates showed low abilities to invade human epithelial Caco-2 cells. However, 4 of the 13 isolates were able to invade Caco-2 cells like reference strains containing complete InlA. Sequencing of inlA and Western blot analysis confirmed that truncated InlA was expressed in the 10 L. monocytogenes strains which were isolated from food. This PCR-RFLP method allowed us to identify 10 new strains expressing a truncated internalin. Based on the results obtained in this study, the PCR-RFLP method seems to be an interesting method for rapidly screening L. monocytogenes strains deficient in the ability to invade Caco-2 cells when a sizeable number of strains are studied.

Restriction fragment length polymorphism mapping of quantitative trait loci for malaria parasite susceptibility in the mosquito Aedes aegypti.

Genetics ◽  
1995 ◽  
Vol 139 (4) ◽  
pp. 1711-1717
Author(s):  
D W Severson ◽  
V Thathy ◽  
A Mori ◽  
Y Zhang ◽  
B M Christensen
Keyword(s):  
Quantitative Trait Loci ◽  
Aedes Aegypti ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Quantitative Trait ◽  
Dominance Effect ◽  
Phenotypic Variance ◽  
Trait Loci ◽  
Two Populations ◽  
Restriction Fragment Length

Abstract Susceptibility of the mosquito Aedes aegypti to the malarial parasite Plasmodium gallinaceum was investigated as a quantitative trait using restriction fragment length polymorphisms (RFLP). Two F2 populations of mosquitoes were independently prepared from pairwise matings between a highly susceptible and a refractory strain of A. aegypti. RFLP were tested for association with oocyst development on the mosquito midgut. Two putative quantitative trait loci (QTL) were identified that significantly affect susceptibility. One QTL, pgs[2,LF98], is located on chromosome 2 and accounted for 65 and 49% of the observed phenotypic variance in the two populations, respectively. A second QTL, pgs[3,MalI], is located on chromosome 3 and accounted for 14 and 10% of the observed phenotypic variance in the two populations, respectively. Both QTL exhibit a partial dominance effect on susceptibility, wherein the dominance effect is derived from the refractory parent. No indication of epistasis between these QTL was detected. Evidence suggests that either a tightly linked cluster of independent genes or a single locus affecting susceptibility to various mosquito-borne parasites and pathogens has evolved near the LF98 locus; in addition to P. gallinaceum susceptibility, this general genome region has previously been implicated in susceptibility to the filarial nematode Brugia malayi and the yellow fever virus.

Linkage map organization of expressed sequence tags and sequence tagged sites in the mosquito, Aedes aegypti

2002 ◽  
Vol 11 (4) ◽  
pp. 371-378 ◽  
Author(s):  
D. W. Severson ◽  
J. K. Meece ◽  
D. D. Lovin ◽  
G. Saha ◽  
I. Morlais
Keyword(s):  
Aedes Aegypti ◽  
Linkage Map ◽  
Expressed Sequence Tags ◽  
Sequence Tagged Sites ◽  
Expressed Sequence
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