Quantitative trait loci influencing chemical and sensory characteristics of eating quality in sweet corn

Genome ◽  
1996 ◽  
Vol 39 (1) ◽  
pp. 40-50 ◽  
Author(s):  
F. Azanza ◽  
Y. Tadmor ◽  
B. P. Klein ◽  
T. R. Rocheford ◽  
J. A. Juvik
Keyword(s):  
Quantitative Trait Loci ◽  
Sensory Evaluation ◽  
Quantitative Trait ◽  
Dimethyl Sulfide ◽  
Sweet Corn ◽  
Sensory Response ◽  
Rflp Markers ◽  
Eating Quality ◽  
Trait Loci ◽  
Single Factor Analysis

This study was conducted to ascertain the chromosomal location and magnitude of effect of quantitative trait loci (QTL) associated with the chemical and sensory properties of sweet corn (Zea mays L.) eating quality. Eighty-eight RFLPs, 3 cloned genes (sh1, sh2, and dhn1), and 2 morphological markers (a2 and se1) distributed throughout the sweet corn genome were scored in 214 F2:3 families derived from a cross between the inbreds W6786su1Se1 and IL731Asu1se1. Kernel properties associated with eating quality (kernel tenderness and starch, phytoglycogen, sucrose, and dimethyl sulfide concentrations) were quantified on F2:3 sib-pollinated ears harvested at 20 days after pollination. Sensory evaluation was conducted on a subset of 103 F2:3 families to determine intensity of attributes associated with sweet corn eating quality (corn aroma, grassy aroma, sweetness, starchiness, grassy flavor, crispness, tenderness, and juiciness) and overall liking. Single factor analysis of variance revealed significant QTL for all these traits, which accounted for from 3 to 42% of the total phenotypic variation. A proportion of the RFLP markers associated with human sensory response were also found to be associated with kernel characteristics. To our knowledge this is the first report of the identification of QTL associated with human flavor preferences in any food crop. Key words : sweet corn, RFLP, quantitative trait loci, eating quality, sensory evaluation.

Detection of Quantitative Trait Loci for Stickiness and Appearance Based on Eating Quality Test in japonica Rice Cultivar

2006 ◽  
Vol 8 (2) ◽  
pp. 39-47 ◽  
Author(s):  
Isao Tanaka ◽  
Asako Kobayashi ◽  
Katsura Tomita ◽  
Yoshinobu Takeuchi ◽  
Masumi Yamagishi ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Rice Cultivar ◽  
Eating Quality ◽  
Japonica Rice ◽  
Quality Test ◽  
Trait Loci

Molecular mapping of quantitative trait loci in japonica rice

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 395-403 ◽  
Author(s):  
Edilberto D. Redoña ◽  
David J. Mackill
Keyword(s):  
Oryza Sativa ◽  
Quantitative Trait Loci ◽  
Qtl Mapping ◽  
Phenotypic Variation ◽  
Quantitative Trait ◽  
Rflp Markers ◽  
Seedling Vigor ◽  
Trait Loci ◽  
Rice Chromosomes ◽  
Molecular Maps

Rice (Oryza sativa L.) molecular maps have previously been constructed using interspecific crosses or crosses between the two major subspecies: indica and japonica. For japonica breeding programs, however, it would be more suitable to use intrasubspecific crosses. A linkage map of 129 random amplified polymorphic DNA (RAPD) and 18 restriction fragment length polymorphism (RFLP) markers was developed using 118 F2 plants derived from a cross between two japonica cultivars with high and low seedling vigor, Italica Livorno (IL) and Labelle (LBL), respectively. The map spanned 980.5 cM (Kosambi function) with markers on all 12 rice chromosomes and an average distance of 7.6 cM between markers. Codominant (RFLP) and coupling phase linkages (among RAPDs) accounted for 79% of total map length and 71% of all intervals. This map contained a greater percentage of markers on chromosome 10, the least marked of the 12 rice chromosomes, than other rice molecular maps, but had relatively fewer markers on chromosomes 1 and 2. We used this map to detect quantitative trait loci (QTL) for four seedling vigor related traits scored on 113 F3 families in a growth chamber slantboard test at 18 °C. Two coleoptile, five root, and five mesocotyl length QTLs, each accounting for 9–50% of the phenotypic variation, were identified by interval analysis. Single-point analysis confirmed interval mapping results and detected additional markers significantly influencing each trait. About two-thirds of alleles positive for the putative QTLs were from the high-vigor parent, IL. One RAPD marker (OPAD13720) was associated with a IL allele that accounted for 18.5% of the phenotypic variation for shoot length, the most important determinant of seedling vigor in water-seeded rice. Results indicate that RAPDs are useful for map development and QTL mapping in rice populations with narrow genetic base, such as those derived from crosses among japonica cultivars. Other potential uses of the map are discussed. Key words : QTL mapping, RAPD, RFLP, seedling vigor, japonica, Oryza sativa.

Mapping quantitative trait loci underlying the cooking and eating quality of rice using a DH population

2005 ◽  
Vol 15 (2) ◽  
pp. 117-124 ◽  
Author(s):  
Rui Tian ◽  
Gong-Hao Jiang ◽  
Li-Huan Shen ◽  
Ling-Qiang Wang ◽  
Yu-Qing He
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Eating Quality ◽  
Dh Population ◽  
Trait Loci ◽  
Cooking And Eating Quality

Genetic resolution and verification of quantitative trait loci for flowering and plant height with recombinant inbred lines of maize

Genome ◽  
1996 ◽  
Vol 39 (5) ◽  
pp. 957-968 ◽  
Author(s):  
David F. Austin ◽  
Michael Lee
Keyword(s):  
Quantitative Trait Loci ◽  
Plant Height ◽  
Quantitative Trait ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Length Polymorphisms ◽  
Trait Loci ◽  
Ear Height ◽  
Single Factor Analysis

Recombinant inbred (RI) lines offer several advantages for detecting quantitative trait loci (QTLs), including increased precision of trait measurements, power for detection of additive effects, and resolution of linked QTLs. This study was conducted to detect and characterize QTLs in maize for flowering and plant height and to compare QTL detection in an early (F2:3) generation of the same population. One hundred and eighty-six RIs from a cross between inbred lines Mo17 and H99 were evaluated in a replicated field experiment and analyzed at 101 loci detected by restriction fragment length polymorphisms. QTLs were identified by single-factor analysis of variance. A total of 59 QTLs were detected for plant height, ear height, top height, anthesis, silk emergence, and anthesis to silk interval. Individual QTLs explained 2.2–15.4% of trait variation, and multiple models including all QTLs detected for a trait explained up to 52.5% of the phenotypic variation. Comparison of QTLs detected with 150 F2:3 lines from the same population indicated that 16 (70%) of the 23 F2:3 QTLs were also observed in the F6:7 generation. Parental effects were consistent across generations. At 14 of the 16 QTLs detected in both generations, genetic effects were smaller in the F6:7. Also, some QTLs detected in the F2:3 were resolved into multiple linked QTLs in the F6:7, indicating the additional power of RI populations for mapping, with important implications for marker-assisted selection as well as map-based cloning of QTLs. Key words : Zea mays, RFLP, plant breeding, genetics, recombination.

Identification of stably expressed quantitative trait loci for cooked rice elongation in non-Basmati varieties

Genome ◽  
2008 ◽  
Vol 51 (2) ◽  
pp. 104-112 ◽  
Author(s):  
L. L. Liu ◽  
X. Y. Yan ◽  
L. Jiang ◽  
W. W. Zhang ◽  
M. Q. Wang ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Recombinant Inbred Lines ◽  
Basmati Rice ◽  
Eating Quality ◽  
Chromosome 2 ◽  
Elongation Ratio ◽  
Substitution Lines ◽  
Chromosome Segment Substitution Lines ◽  
Trait Loci

The elongation of the cooked grain determines the cooking and eating quality of Basmati rice. The identification of stable quantitative trait loci (QTLs), especially those from non-Basmati types, will extend the genetic basis of the Basmati type and facilitate the breeding of high-quality varieties. A set of recombinant inbred lines derived from an indica × japonica hybrid was used to identify QTLs controlling the elongation ratio (ER), elongation index (EI), and water absorption (WA) of the cooked grain. Three ER QTLs on chromosomes 2, 4, and 12, two EI QTLs on chromosomes 2 and 5, and two WA QTLs on chromosomes 2 and 6 were detected. Four of these QTLs were validated using a set of established chromosome segment substitution lines. The genetic effect of qER-2 was explored in an analysis of segregating generations, using 8 newly developed simple sequence repeat markers. Two tightly linked loci (qER-2a and qER-2b) were identified on chromosome 2.

Dynamic quantitative trait loci for salt stress components on chromosome 1 of rice

2010 ◽  
Vol 37 (7) ◽  
pp. 634 ◽  
Author(s):  
Tanveer Ul Haq ◽  
John Gorham ◽  
Javaid Akhtar ◽  
Nasim Akhtar ◽  
Katherine A. Steele
Keyword(s):  
Salt Stress ◽  
Quantitative Trait Loci ◽  
Linkage Map ◽  
Quantitative Trait ◽  
Recombinant Inbred Lines ◽  
Leaf Age ◽  
Chromosome 1 ◽  
Rflp Markers ◽  
Rice Varieties ◽  
Trait Loci

Rice varieties Co39 and Moroberekan differ for leaf Na+ concentrations when grown at moderate salinity (100–150 mol m–3 NaCl; 10 : 1 or 20 : 1 Na+ to Ca2+ ratio). Recombinant inbred lines (RILs) from a cross between them were used to map quantitative trait loci (QTL) under salt stress over several weeks. Two experiments (conducted with 170 and 96 RILs, and a linkage map of 126 RFLP markers) identified a major effect on QTL for leaf Na+ concentration and K+ : Na+ ratio on chromosome 1 in a region corresponding to 11.07–14.6 Mbp. No leaf Cl– QTL were detected. In a third experiment, leaves and sheaths were harvested after 7 and 21 days at 100 mol m–3 NaCl. The linkage map of chromosome 1 was improved by the addition of 28 microsatellite markers, which resolved distinct QTL for Na+ and K+ concentrations, and K+ : Na+ ratio. After 7 days’ stress, the most significant QTL were in the region of 11.56–12.66 Mbp. The highest Na+ concentrations were recorded in the sheaths. Na+ concentration QTL were detected for leaves, but not for sheaths. After 21 days’ stress, the region containing the most significant QTL extended to 11.07 Mbp in leaves and in sheaths. A QTL for the ratio of leaf Na+ to sheath Na+ concentrations was found at 11.39–12.39 Mbp. These findings suggest that multiple genes in this region are involved in the response to salinity, and their impact is dynamic according to stress duration, and leaf age and type.

Molecular Markers Linked to Quantitative Trait Loci for Anthracnose Resistance in Tomato

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 515a-515 ◽  
Author(s):  
John R. Stommel ◽  
Yiping Zhang
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Marker Assisted Breeding ◽  
Anthracnose Resistance ◽  
Trait Loci ◽  
Marker Loci ◽  
Single Factor Analysis ◽  
Potential Use

Random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers linked to quantitative trait loci (QTL) involved in tomato anthracnose resistance were identified in an F2 population of tomato (Lycopersicon esculentum) segregating for anthracnose resistance. The F2 population was developed from the cross of an unadapted and small-fruited, but highly anthracnose-resistant L. esculentum accession and an adapted, but anthracnose-susceptible processing type tomato. Resistance to anthracnose caused by the fungal pathogen Colletotrichum coccodes is estimated to be controlled by at least three genes or chromosomal regions in this cross. One-thousand RAPD random primers and 64 AFLP primer pairs were screened for polymorphisms between the parental lines. Primers or primer pairs which differentiated the anthracnose resistant and susceptible parents were utilized to screen the F2 population for detection of QTL. Using single-factor analysis of variance, a number of markers, including six unmapped RAPD markers were identified that were significantly associated with resistance. Mapping of marker loci and their potential use in marker assisted breeding will be discussed.

scholarly journals Quantitative Trait Loci in Sweet Corn Associated with Partial Resistance to Stewart's Wilt, Northern Corn Leaf Blight, and Common Rust

2001 ◽  
Vol 91 (3) ◽  
pp. 293-300 ◽  
Author(s):  
A. F. Brown ◽  
J. A. Juvik ◽  
J. K. Pataky
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Partial Resistance ◽  
Sweet Corn ◽  
Phenotypic Variability ◽  
Leaf Blight ◽  
Northern Corn Leaf Blight ◽  
Trait Loci ◽  
Stewart's Wilt ◽  
Corn Leaf Blight

Partial resistance to Stewart's wilt (Erwina stewartii, syn. Pantoea stewartii), northern corn leaf blight (NCLB) (Exserohilum turcicum), and common rust (Puccinia sorghi) was observed in an F2:3 population developed from a cross between the inbred sweet corn lines IL731a and W6786. The objective of this study was to identify quantitative trait loci (QTL) associated with partial resistance using restriction fragment length polymorphic markers. Phenotypic data were collected for 2 years for Stewart's wilt, NCLB, and common rust but, due to significant family-environment interaction, analysis was conducted individually on data from each year. In 2 years of evaluation for the three diseases, a total of 33 regions in the maize genome were associated with partial resistance describing from 5.9 to 18% of the total phenotypic variability. Of six regions common in both years, three were associated with partial resistance to Stewart's wilt (chromosomes 4:07, 5:03, and 6:04), one was associated with NCLB (chromosome 9:05), and two were associated with common rust (chromosomes 2:04 and 3:04). The rust QTL on 3S mapped to within 20 cM of the rp3 locus and explained 17.7% of the phenotypic variability. Some of the QTL associated with partial resistance to the three diseases have been reported previously, and some are described here for the first time. Results suggest it may be possible to consolidate QTL from various elite backgrounds in a manner analogous to the pyramiding of major resistance genes. We also report here on two QTL associated with anthocyanin production on chromosomes 10:6 and 5:03 in the general location of the a2 gene.

scholarly journals RFLP mapping in soybean: association between marker loci and variation in quantitative traits.

Genetics ◽  
1990 ◽  
Vol 126 (3) ◽  
pp. 735-742 ◽  
Author(s):  
P Keim ◽  
B W Diers ◽  
T C Olson ◽  
R C Shoemaker
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Quantitative Traits ◽  
Glycine Soja ◽  
Interspecific Cross ◽  
Field Trials ◽  
Rflp Markers ◽  
Progeny Testing ◽  
Trait Loci ◽  
Marker Loci

Abstract We have constructed a genetic map for soybean and identified associations between genetic markers and quantitative trait loci. One-hundred-fifty restriction fragment length polymorphisms (RFLPs) were used to identify genetic linkages in an F2 segregating population from an interspecific cross (Glycine max x Glycine soja). Twenty-six genetic linkage groups containing ca. 1200 recombination units are reported. Progeny-testing of F2-derived families allowed quantitative traits to be evaluated in replicated field trials. Genomic regions, which accounted for a portion of the genetic variation (R2 = 16 to 24%) in several reproductive and morphological traits, were linked to RFLP markers. Significant associations between RFLP markers and quantitative trait loci were detected for eight of nine traits evaluated. The ability to identify genes within a continuously varying trait has important consequences for plant breeding and for understanding evolutionary processes.

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