Genetic and cytological mapping of a "Y–2" translocation in the Mediterranean fruit fly Ceratitis capitata

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1091-1097 ◽  
Author(s):  
Jorge L. Cladera ◽  
M. Alejandra Delprat
Keyword(s):  
Y Chromosome ◽  
Polytene Chromosome ◽  
Large Scale ◽  
Fruit Fly ◽  
Chromosome 2 ◽  
Genetic Sexing ◽  
Male Recombination ◽  
Late Stages ◽  
Breakpoint Mapping ◽  
Determining Factor

In this paper we analyze genetically and cytologically a Y – chromosome 2 translocation with several markers, some of which are potentially useful for large scale sex separation. The breakpoint of this Y–2 translocation is located at region 6B on the trichogene polytene chromosome map. It was found that, in strains carrying this TY–2, only 40% of the fertilized eggs survived to the adult stage, 26% of them dying as embryos, 27% as larvae, and 7% as pupae. Early lethality is explained by the nonviability of adjacent-1 products of meiosis containing a deletion of section 1A–6B. The reciprocal segregation products, carrying this chromosome segment in triplicate, survive until late stages. By analyzing the phenotype of these individuals we conclude that all markers used in this study are located outside the triplicated region and that the male determining factor is not included in the piece of the Y chromosome translocated to chromosome 2. The male recombination frequencies of several genes located on chromosome 2 relative to the breakpoint of translocation T5038 have also been studied here.Key words: genetic sexing strain, Medfly polytene chromosome, adjacent-1 product, translocation breakpoint mapping.

Cytogenetic characterization of a genetic sexing strain in Ceratitis capitata

Genome ◽  
1991 ◽  
Vol 34 (4) ◽  
pp. 606-611 ◽  
Author(s):  
A. Zacharopoulou ◽  
M. E. Riva ◽  
A. Malacrida ◽  
G. Gasperi
Keyword(s):  
Y Chromosome ◽  
Ceratitis Capitata ◽  
Chromosomal Rearrangements ◽  
Polytene Chromosomes ◽  
Chromosome 2 ◽  
Genetic Sexing ◽  
Cytogenetic Characterization ◽  
Cytological Characteristics ◽  
Genetic Sexing Strain

A genetic sexing strain of Ceratitis capitata, carrying a null mutation for ADH activity linked to the Y chromosome, was analysed cytogenetically. In addition to an insertion of a large part of the Y chromosome into chromosome 2, this strain carries two other chromosomal rearrangements, a deletion in the second chromosome and a reciprocal translocation involving chromosomes 2 and 4. The progeny of the T(2;4) translocation heterozygote with unbalanced chromosome constitution can survive up to the larval and (or) to the adult stage. These cytological characteristics are discussed in relation to the genetic sexing behaviour of this line.Key words: Ceratitis capitata, polytene chromosomes, genetic sexing.

Sex chromosome aneuploidy in a male-linked translocation in Ceratitis capitata

1986 ◽  
Vol 28 (1) ◽  
pp. 161-167 ◽  
Author(s):  
M. Zapater ◽  
A. S. Robinson
Keyword(s):  
Alcohol Dehydrogenase ◽  
Sex Determination ◽  
Y Chromosome ◽  
Ceratitis Capitata ◽  
Sex Chromosome ◽  
Translocation Line ◽  
Chromosome 2 ◽  
Genetic Sexing ◽  
Sex Chromosome Aneuploidy ◽  
Cytological Data

A translocation line in Ceratitis capitata (2n = 12) linking the male-determining Y chromosome with the autosome carrying the alcohol dehydrogenase (Adh) locus was examined to assign the locus to its chromosome. Adh was assigned to chromosome 2, the largest autosome, representing the first correlation between genetic and cytological data in this species. During the study, male adults with 11 and 12 chromosomes were identified in the population and a model was proposed to account for the appearance of these individuals. Predictions made from the model were in general verified by the subsequent experiments. It was shown that 11-chromosome males were more fertile and produced more males than 12-chromosome males. Eleven-chromosome males produced 11- and 12-chromosome progeny, whereas 12-chromosome males produced only 12-chromosome male progeny. The results are discussed with relation to sex determination in this species and to the use of male-linked translocations for the development of genetic sexing techniques.Key words: Ceratitis, male determination, translocation, aneuploidy.

Improved stability of genetic sex-separation strains for the Mediterranean fruit fly, Ceratitis capitata

Genome ◽  
1994 ◽  
Vol 37 (1) ◽  
pp. 72-82 ◽  
Author(s):  
G. Franz ◽  
E. Gencheva ◽  
Ph. Kerremans
Keyword(s):  
Ceratitis Capitata ◽  
Recombination Frequency ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Temperature Sensitive ◽  
Genetic Sexing ◽  
Male Recombination ◽  
Comparable Level ◽  
Improved Stability ◽  
Genetic Sexing Strains

In the existing genetic sexing strains for the medfly, Ceratitis capitata, male recombination leads to breakdown of the sexing mechanism under mass rearing conditions. The rate of breakdown depends on the recombination frequency and on the fitness of the recombinants. We have tested two different sexing genes, white pupa and a temperature sensitive lethal, in combination with the translocation T(Y;5)30C. Both sexing strains broke down, although at very different rates. In the case of the white pupa strain, 3.5% recombinants were observed after rearing the strain for 15 generations. The second strain, utilizing white pupa and the temperature sensitive lethal as selectable markers, already reached a comparable level after six generations and was broken down completely in the ninth generation. In these strains the frequency of recombination is high because the breakpoint of T(Y;5)30C and the sexing gene(s) are far apart. To remedy the situation, we have isolated four new translocations with breakpoints located closer to the sexing genes. Mass rearing was simulated for several generations with strains based on these translocations and no breakdown was observed under the conditions used.Key words: medfly, sterile insect technique, genetic sexing, recombination.

scholarly journals Slow Larvae Mutant and Its Potential to Improve the Pupal Color-Based Genetic Sexing System in Mexican Fruit Fly, (Diptera: Tephritidae)

2019 ◽  
Vol 112 (4) ◽  
pp. 1604-1610
Author(s):  
José S Meza ◽  
Carlos Cáceres ◽  
Kostas Bourtzis
Keyword(s):  
Large Scale ◽  
Principal Component ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Anastrepha Ludens ◽  
Wild Type ◽  
Mexican Fruit Fly ◽  
Genetic Sexing ◽  
Scale Population ◽  
Fly Control

Abstract For many years, an area-wide fruit fly control campaign against the Mexican fruit fly, Anastrepha ludens (Loew) has been implemented in some regions of Mexico and Texas, using the sterile insect technique (SIT) as its principal component. To improve the efficiency of the SIT, a genetic sexing strain based on black pupae mutation (bp) was developed for A. ludens, namely, ‘Tapachula-7’ (Tap-7 genetic sexing strains [GSSs]). This strain was introduced into the AW-IPM program recently and allows male-only releases for SIT applications. Here, we report the genetic and biological characterization of a new mutation, slow larvae (sl), which was introduced to the original translocation of the Tap-7 GSS resulting in two new GSS (slow-7 and Tap/slow-7). In both GSSs, the translocated wild-type males emerge from brown pupae that develop faster than females. The females are homozygous for sl mutation in the slow-7 GSS and homozygous for sl and bp mutations in the Tap/slow-7 GSS, reaching larval maturity 2 d after most of the wild-type males, allowing the separation of most males during pupariation. The potential use of the slow-7 and Tap/slow-7 GSSs in mass rearing and large-scale population suppression programs is discussed.

scholarly journals Genetic analysis of medfly populations in an area of sterile insect technique applications

2021 ◽  
Author(s):  
Rubén Sancho ◽  
Ana Guillem-Amat ◽  
Elena López-Errasquín ◽  
Lucas Sánchez ◽  
Félix Ortego ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Sterile Insect Technique ◽  
Ceratitis Capitata ◽  
Natural Populations ◽  
Low Frequency ◽  
Fruit Fly ◽  
Wild Populations ◽  
Genetic Sexing ◽  
Environmental Implications ◽  
Genetic Sexing Strains

AbstractThe sterile insect technique (SIT) is widely used in integrated pest management programs for the control of the Mediterranean fruit fly (medfly), Ceratitis capitata. The genetic interactions between the released individuals from the genetic sexing strains (GSS), used for SIT applications worldwide, and wild individuals have not been studied. Under the hypothesis that a number of Vienna GSS individuals released to the field might not be completely sterile and may produce viable offspring, we have analyzed medfly Spanish field populations to evaluate the presence of Vienna strain genetic markers. To this goal, we have used contrasted nuclear and mitochondrial genetic markers, and two novel sets of nuclear polymorphisms with the potential to be markers to discriminate between Vienna and wild individuals. Nuclear Vienna markers located on the 5th chromosome of Vienna males have been found in 2.2% (19 from 875) of the Spanish wild medfly females captured at the area where SIT is applied. In addition, a female-inherited mitochondrial Vienna marker has been found in two from the 19 females showing nuclear Vienna markers. The detection of several of these markers in single individuals represents evidence of the introgression of Vienna strain into natural populations. However, alternative explanations as their presence at low frequency in wild populations in the studied areas cannot be fully discarded. The undesired release of non-fully sterile irradiated GSS individuals into the field and their interactions with wild flies, and the potential environmental implications should be taken into account in the application of the SIT.

scholarly journals Determining the Sterilization Doses under Hypoxia for the Novel Black Pupae Genetic Sexing Strain of Anastrepha fraterculus (Diptera, Tephritidae)

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 308
Author(s):  
Paloma Della Giustina ◽  
Thiago Mastrangelo ◽  
Sohel Ahmad ◽  
Gabriel Mascarin ◽  
Carlos Caceres
Keyword(s):  
Fruit Fly ◽  
South American ◽  
Response Curves ◽  
Genetic Sexing ◽  
Egg Hatch ◽  
Anastrepha Fraterculus ◽  
Common Strategy ◽  
Genetic Sexing Strain ◽  
South American Fruit Fly ◽  
First Time

A common strategy used to maintain sterile fly quality without sacrificing sterility is to irradiate the insects under an oxygen-reduced atmosphere. So far, sterilizing doses for the South American fruit fly Anastrepha fraterculus have only been determined under normoxia. Our study reports for the first time the dose-sterility response under hypoxia for two different A. fraterculus strains. The pupae were derived from a bisexual strain (a Brazilian-1 population) and a recently developed genetic sexing strain (GSS-89). Two hours prior to irradiation, pupae were transferred to sealed glass bottles and irradiated when oxygen concentration was below 3%. Four types of crosses with nonirradiated flies of the bisexual strain were set to assess sterility for each radiation dose. For males from both strains, Weibull dose–response curves between radiation doses and the proportion of egg hatch, egg-to-pupa recovery, and recovery of adults were determined. The GSS males revealed high sterility/mortality levels compared to males from the bisexual strain at doses < 40 Gy, but a dose of 74 Gy reduced egg hatch by 99% regardless of the male strain and was considered the sterilizing dose. The fertility of irradiated females was severely affected even at low doses under hypoxia.

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