Amplified extrachromosomal elements containing c-Myc and Pvt 1 in a mouse plasmacytoma

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 780-785 ◽  
Author(s):  
S. Mai ◽  
J. Hanley-Hyde ◽  
A. Coleman ◽  
D. Siwarski ◽  
K. Huppi
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Key Words ◽  
Mouse Chromosome ◽  
Plasma Cell ◽  
Cell Tumor ◽  
Chromosome 15 ◽  
Chromosomal Alterations ◽  
Extrachromosomal Elements

After adaptation of a mouse plasma cell tumor, MOPC265, to culture, we have found several unique chromosomal alterations in addition to the T(12;15) translocation and trisomy 11 frequently observed in plasmacytomas. Among these alterations is a specific coamplification of the c-Myc and Pvt 1 gene loci from mouse chromosome 15. Further analysis by fluorescence in situ hybridization demonstrates that the amplicons of c-Myc and Pvt 1 exist as extrachromosomal elements as well as within intact chromosomes. Most importantly, the presence of both Pvt 1 and c-Myc in these extrachromosomal elements indicates ongoing coselection for these loci in the propagation of MOPC265.Key words: amplification, c-Myc, Pvt 1, plasmacytoma.

Cytogenetic and fluorescence in situ hybridization studies in 60 patients with multiple myeloma and plasma cell leukemia

2004 ◽  
Vol 148 (1) ◽  
pp. 71-76 ◽  
Author(s):  
Elisabet Lloveras ◽  
Isabel Granada ◽  
Lurdes Zamora ◽  
Blanca Espinet ◽  
Lourdes Florensa ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Plasma Cell ◽  
Plasma Cell Leukemia ◽  
Cell Leukemia

Fluorescence In Situ Hybridization Studies in 22 Cases of Plasma Cell Leukemia.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4461-4461
Author(s):  
Jianyong Li ◽  
Wei Xu ◽  
Lijuan Chen ◽  
Yu Zhu ◽  
Jinlan Pan ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Plasma Cell ◽  
Cell Leukemia ◽  
Molecular Cytogenetic ◽  
Cytogenetic Aberrations ◽  
Plasma Cell Disorder ◽  
Igh Rearrangement ◽  
Cell Disorder

Abstract Background Plasma cell leukemia (PCL) is a rare malignant plasma cell disorder that usually carries an aggressive course with a rapidly fatal outcome. Cytogenetic studies performed on plasma cell disorder are scarce and difficult because of the low proliferation rate of plasma cells. Fluorescence in situ hybridization (FISH) analysis is an attractive alternative for evaluation of numerical and structural chromosomal changes in PCL. Methods Interphase FISH (I-FISH) and two different specific probes for the regions containing 13q14.3 (D13S319) or 14q32 (IGHC/IGHV) were used to detect 13q14 deletion [del(13q14)] or IgH rearrangement in 22 PCL patients. For patients with IgH rearrangement, probes for IgH(JH) and 11q13 (CCND1) or 4p16 (FGFR3) were used to detect t(11;14)(q13;q32) or t(4;14)(p16;q32). Results Molecular cytogenetic aberrations were found in 19 of 22 (86.4%) PCL patients. Del(13q14) was detected in 13 cases (59.1%), and IgH rearrangement in 17 (77.3%) patients including 7 with t(11;14) and 3 with t(4;14). 14q32 rearrangement and 13q14 deletion were found concurrently in 11 cases (50%). Conclusions Chromosomal abnormalities are frequent in PCL. The most frequent aberrations among the cases was the 14q32 rearrangement and 13q14 deletion. I-FISH technique is useful to detect molecular cytogenetic aberrations and should be used in the routine evaluation of PCL.

scholarly journals Prenatal Confirmation of the Translocation between Chromosome 15 and Y-Chromosome by Fluorescence in situ Hybridization.

1999 ◽  
Vol 187 (3) ◽  
pp. 285-289 ◽  
Author(s):  
Yukihito Fukada ◽  
Takehiko Yasumizu ◽  
Atsuhito Amemiya ◽  
Keiko Kohno ◽  
Motoi Takizawa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Y Chromosome ◽  
Chromosome 15
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