Agrobacterium-mediated transformation of large-flowered purslane (Portulaca grandiflora H.)

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 752-756 ◽  
Author(s):  
Badr-Din Rossi-Hassani ◽  
Fadoua Bennani ◽  
Jean-Pierre Zryd
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Dna Hybridization ◽  
Segregation Analysis ◽  
Binary Vector ◽  
Dna Transfer ◽  
Antibiotic Resistant ◽  
Portulaca Grandiflora ◽  
Resistant Progeny ◽  
Vector Dna ◽  
Selection Of

Transformation of Portulaca grandiflora has been developed with Agrobacterium tumefaciens strains A281 and T1272. Transformation was assessed by the following criteria: selection of hormone independent callus, antibiotic-resistant callus, and transgenic antibiotic-resistant plants. In addition, DNA hybridization analysis demonstrated that the DNA from tumor lines contained sequences homologous to binary vector T-DNA of strain A281. Following transformation with strain T1272, segregation analysis of the progeny of transgenic plants showed that the transgene was inherited in a Mendelian manner. The kanamycin-resistant progeny tested contained the T-DNA sequence of the strain T1272.Key words: Agrobacterium tumefaciens, binary vector, DNA transfer, regeneration, Portulaca grandiflora.

scholarly journals Tetracycline-Resistant Bacteria Selected from Water and Zebrafish after Antibiotic Exposure

2021 ◽  
Vol 18 (6) ◽  
pp. 3218
Author(s):  
Ana Rita Almeida ◽  
Marta Tacão ◽  
Joana Soares ◽  
Inês Domingues ◽  
Isabel Henriques
Keyword(s):  
Statistical Significance ◽  
Recovery Period ◽  
Antibiotic Resistance Genes ◽  
Antibiotic Use ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Potential Impact ◽  
Susceptibility Profiles ◽  
Selection Of ◽  
Significant Mortality

The emergence of antibiotic-resistant pathogens due to worldwide antibiotic use is raising concern in several settings, including aquaculture. In this work, the selection of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) was evaluated after exposure of zebrafish to oxytetracycline (OTC) for two months, followed by a recovery period. The selection of ARB in water and fish was determined using selective media. The abundance of tetA genes was estimated through qPCR. Higher prevalence of ARB was measured in all samples exposed to the antibiotic when compared to control samples, although statistical significance was only achieved five days after exposure. Isolates recovered from samples exposed to the antibiotic were affiliated with Pseudomonas and Stenotrophomonas. Various antibiotic susceptibility profiles were detected and 37% of the isolates displayed multidrug resistance (MDR). The selection of the tetA gene was confirmed by qPCR at the highest OTC concentration tested. Two MDR isolates, tested using zebrafish embryos, caused significant mortality, indicating a potential impact on fish health and survival. Overall, our work highlights the potential impact of antibiotic contamination in the selection of potential pathogenic ARB and ARGS.

Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens

Nature ◽  
1985 ◽  
Vol 318 (6047) ◽  
pp. 624-629 ◽  
Author(s):  
Scott E. Stachel ◽  
Eric Messens ◽  
Marc Van Montagu ◽  
Patricia Zambryski
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Cells ◽  
Dna Transfer ◽  
Signal Molecules

Factors Influencing T-DNA Transfer into Wheat and Barley Cells by Agrobacterium Tumefaciens

1998 ◽  
Vol 26 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Guangqin Guo ◽  
Frank Maiwald ◽  
Petra Lorenzen ◽  
Hans-Henning Steinbiss
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Dna Transfer ◽  
Factors Influencing

Interactions between micro-organisms on skin

1980 ◽  
Vol 79 (1-3) ◽  
pp. 185-191
Author(s):  
W. C. Noble
Keyword(s):  
Skin Lesion ◽  
Secondary Metabolites ◽  
Bacterial Flora ◽  
Antibiotic Resistant ◽  
General Terms ◽  
Micro Organisms ◽  
Selection Of

SynopsisThe mechanisms of interaction are discussed in general terms; this is followed by a review of interaction which may occur in tinea paedis.The production of penicillin-like and other antibiotics by dermatophyte fungi in lesions of athletes foot may result in the selection of an antibiotic-resistant bacterial flora which, perhaps by production of antibiotic or other secondary metabolites, can reduce the viability of the fungi. The effect on the host is to bring about a change from a fungal skin lesion which is dry and scaly to a bacterial lesion which is macerated and malodorous.

Phylogeny of the family Pasteurellaceae based on rpoB sequences

2004 ◽  
Vol 54 (4) ◽  
pp. 1393-1399 ◽  
Author(s):  
Bożena Korczak ◽  
Henrik Christensen ◽  
Stefan Emler ◽  
Joachim Frey ◽  
Peter Kuhnert
Keyword(s):  
16S Rdna ◽  
Dna Hybridization ◽  
Gene Encoding ◽  
Phylogenetic Studies ◽  
Study Selection ◽  
Gene Sequence Analysis ◽  
Rdna Sequencing ◽  
The Family ◽  
Type Strains ◽  
Selection Of

Sequences of the gene encoding the β-subunit of the RNA polymerase (rpoB) were used to delineate the phylogeny of the family Pasteurellaceae. A total of 72 strains, including the type strains of the major described species as well as selected field isolates, were included in the study. Selection of universal rpoB-derived primers for the family allowed straightforward amplification and sequencing of a 560 bp fragment of the rpoB gene. In parallel, 16S rDNA was sequenced from all strains. The phylogenetic tree obtained with the rpoB sequences reflected the major branches of the tree obtained with the 16S rDNA, especially at the genus level. Only a few discrepancies between the trees were observed. In certain cases the rpoB phylogeny was in better agreement with DNA–DNA hybridization studies than the phylogeny derived from 16S rDNA. The rpoB gene is strongly conserved within the various species of the family of Pasteurellaceae. Hence, rpoB gene sequence analysis in conjunction with 16S rDNA sequencing is a valuable tool for phylogenetic studies of the Pasteurellaceae and may also prove useful for reorganizing the current taxonomy of this bacterial family.

scholarly journals An Improved Binary Vector and Escherichia coli Strain for Agrobacterium tumefaciens-Mediated Plant Transformation

2016 ◽  
Vol 6 (7) ◽  
pp. 2195-2201 ◽  
Author(s):  
Michael R. Watson ◽  
Yu-fei Lin ◽  
Elizabeth Hollwey ◽  
Rachel E. Dodds ◽  
Peter Meyer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Agrobacterium Tumefaciens ◽  
Plant Transformation ◽  
Binary Vector ◽  
Coli Strain

scholarly journals Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae.

1995 ◽  
Vol 14 (13) ◽  
pp. 3206-3214 ◽  
Author(s):  
P. Bundock ◽  
A. den Dulk-Ras ◽  
A. Beijersbergen ◽  
P.J. Hooykaas
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Agrobacterium Tumefaciens ◽  
Dna Transfer
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