Construction of an intraspecific integrated linkage map of pepper using molecular markers and doubled-haploid progenies

Genome ◽  
1995 ◽  
Vol 38 (1) ◽  
pp. 112-121 ◽  
Author(s):  
Véronique Lefebvre ◽  
Alain Palloix ◽  
Carole Caranta ◽  
Edmond Pochard
Keyword(s):  
Linkage Map ◽  
Capsicum Annuum ◽  
Doubled Haploid ◽  
Linkage Groups ◽  
Hypersensitive Resistance ◽  
Integrated Linkage Map ◽  
New Genes ◽  
Rflp Rapd ◽  
Pepper Genome ◽  
Genomic Regions

An integrated molecular linkage map of pepper (Capsicum annuum L.), including mainly RFLP and RAPD markers, has been constructed by alignment of three intraspecific linkage maps generated by segregating doubled-haploid progenies. A total of 85 markers covered approximately 820 cM in 14 linkage groups. Four linkage groups were assigned to 4 chromosomes. Two new genes of agronomic interest were located: L controlling hypersensitive resistance to TMV and up controlling the erect habit of the fruits. The C gene controlling the fruit pungency was more precisely located. This map is estimated to represent from 36 to 59% of the total pepper genome. An examination of segregation data has revealed several genomic regions with aberrant segregation ratios often favouring the agronomic big-fruited parents, particularly in crosses involving the exotic parent CM334, suggesting that these genome regions are subjected to selection during the process of doubled-haploid production. The suitability of doubled-haploid progenies for mapping projects and the differences observed between this intraspecific integrated map with earlier published interspecific pepper maps are discussed.Key words: Capsicum annuum, RFLP, RAPD, integrated linkage map, doubled-haploid progenies.

scholarly journals Genetic linkage map of Phaseolus vulgaris and identification of QTLs responsible for resistance to Xanthomonas axonopodis pv. phaseoli

2003 ◽  
Vol 28 (1) ◽  
pp. 5-10 ◽  
Author(s):  
Amaury S. Santos ◽  
Ricardo E. Bressan-Smith ◽  
Messias G. Pereira ◽  
Rosana Rodrigues ◽  
Claudia F. Ferreira
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Linkage Map ◽  
Linkage Groups ◽  
Resistant Genotype ◽  
Xanthomonas Axonopodis ◽  
Low Degree ◽  
Phenotypic Variations ◽  
Genomic Regions ◽  
High Degree

Common bean (Phaseolus vulgaris) cultivars with a high degree of resistance to Xanthomonas axonopodis pv. phaseoli (Xap) are not available in Brazil. Despite many studies, a low degree of resistance to Xap continues to exist due to its complex genetic inheritance, which is not well known. The objectives of this research were to complement a common bean genetic map based on the cross between a susceptible genotype 'HAB-52' and a resistant genotype 'BAC-6', and to map and analyze genomic regions (quantitative trait loci – QTLs) related to Xap resistance. Eleven linkage groups were determined using 143 RAPD markers, covering 1,234.5 cM of the genome. This map was used to detect QTLs associated with Xap resistance on leaves and pods. The averages of disease severity on leaves (represented by the transformed disease index – TDI) and pods (represented by the diameter of lesion on pods – DLP) were added to the data of the linkage map. Five TDI QTLs and only one LDP QTL were detected. The TDI QTLs were placed in the A, B, G and J linkage groups, with phenotypic variations ranging from 12.7 to 71.6%. The DLP QTL explained 12.9% of the phenotypic variation and was mapped in a distinct linkage group. These results indicate that there are different genes involved in the control of resistance on leaves and pods.

An updated doubled haploid oat linkage map and QTL mapping of agronomic and grain quality traits from Canadian field trials

Genome ◽  
2012 ◽  
Vol 55 (4) ◽  
pp. 289-301 ◽  
Author(s):  
Pirjo Tanhuanpää ◽  
Outi Manninen ◽  
Aaron Beattie ◽  
Peter Eckstein ◽  
Graham Scoles ◽  
...  
Keyword(s):  
Linkage Map ◽  
Doubled Haploid ◽  
Avena Sativa ◽  
Dna Markers ◽  
Grain Quality ◽  
Field Trials ◽  
Diversity Array Technology ◽  
Linkage Groups ◽  
Array Technology ◽  
Reference Map

The first doubled haploid oat linkage map constructed at MTT Agrifood Research Finland was supplemented with additional microsatellites and Diversity Array Technology (DArT) markers to produce a map containing 1058 DNA markers and 34 linkage groups. The map was used to locate quantitative trait loci (QTLs) for 11 important breeding traits analyzed from Finnish and Canadian field trials. The new markers enabled most of the linkage groups to be anchored to the ‘Kanota’ × ‘Ogle’ oat ( Avena sativa L.) reference map and allowed comparison of the QTLs located in this study with those found previously. Two to 12 QTLs for each trait were discovered, of which several were expressed consistently across several environments.

Development of an AFLP-based linkage map and localization of QTLs for seed fatty acid content in condiment mustard (Brassica juncea)

Genome ◽  
2002 ◽  
Vol 45 (6) ◽  
pp. 1203-1215 ◽  
Author(s):  
E Lionneton ◽  
S Ravera ◽  
L Sanchez ◽  
G Aubert ◽  
R Delourme ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linkage Map ◽  
Brassica Juncea ◽  
Doubled Haploid ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Major Gene ◽  
Fatty Acid Content ◽  
Eicosenoic Acid ◽  
Linkage Groups

A genetic linkage map of Brassica juncea based on AFLP and RAPD markers was constructed using 131 F1-derived doubled-haploid (DH) plants from a cross between two mustard lines. The map included 273 markers (264 AFLP, 9 RAPD) arranged on 18 linkage groups, and covered a total genetic distance of 1641 cM; 18.3% of the AFLP markers showed a segregation distortion (P < 0.01). The markers with biased segregation were clustered on seven linkage groups. QTLs for oil contents, palmitic acid (16:0), stearic acid (18:0), oleic acid (18:1), linoleic acid (18:2), linolenic acid (18:3), eicosenoic acid (20:1), and erucic acid (22:1), were mapped on the AFLP linkage map. Correlation studies among fatty acids in the DH population and the localization of QTLs involved in their control indicated that a major gene located on linkage group (LG) 2 controlled the elongation step of erucic acid.Key words: Brassica juncea, doubled haploid, AFLP genetic linkage map, fatty acids, QTL.

scholarly journals Secondary Trisomics and Telotrisomics of Rice: Origin, Characterization, and Use in Determining the Orientation of Chromosome Map

Genetics ◽  
1996 ◽  
Vol 143 (1) ◽  
pp. 517-529
Author(s):  
Kuldeep Singh ◽  
D S Multani ◽  
Gurdev S Khush
Keyword(s):  
Linkage Map ◽  
Large Population ◽  
Marker Genes ◽  
Linkage Groups ◽  
Breeding Behavior ◽  
Specific Chromosome ◽  
Chromosome Map ◽  
Primary Trisomics ◽  
Correct Orientation ◽  
Genetic Segregation

Abstract Secondary trisomics and telotrisomics representing the 12 chromosomes of rice were isolated from the progenies of primary trisomics. A large population of each primary trisomic was grown. Plants showing variation in gross morphology compared to the primary trisomics and disomic sibs were selected and analyzed cytologically at diakinesis and pachytene. Secondary trisomics for both arms of chromosomes 1, 2, 6, 7 and 11 and for one arm of chromosomes 4, 5, 8, 9 and 12 were identified. Telotrisomics for short arm of chromosomes 1, 8, 9 and 10 and for long arms of chromosomes 2, 3 and 5 were isolated. These secondary and telotrisomics were characterized morphologically and for breeding behavior. Secondary trisomics 2n + 1S · 1S, 2n + 1L · 1L, 2n + 2S · 2S, 2n + 2L · 2L, 2n + 6S · 6S, 2n + 6L · 6L and 2n + 7L · 7L are highly sterile, and 2n + 1L · 1L, 2n + 2L · 2L and 2n + 7L · 7L do not set any seed even upon backcrossing. Telotrisomics are fertile and vigorous. Genetic segregation of 43 marker genes was studied in the F2 or backcross progenies. On the basis of segregation data, these genes were delimited to specific chromosome arms. Correct orientation of 10 linkage groups was determined and centromere positions on nine linkage groups were approximated. A revised linkage map of rice is presented.

scholarly journals Mapping QTL for spike fertility and related traits in two doubled haploid wheat (Triticum aestivum L.) populations

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nicole Pretini ◽  
Leonardo S. Vanzetti ◽  
Ignacio I. Terrile ◽  
Guillermo Donaire ◽  
Fernanda G. González
Keyword(s):  
Doubled Haploid ◽  
Marker Assisted Selection ◽  
Triticum Aestivum L ◽  
Physiological Model ◽  
Yield Potential ◽  
Breeding Programs ◽  
Per Se ◽  
Genomic Regions ◽  
Fertility Traits ◽  
Selection Of

Abstract Background In breeding programs, the selection of cultivars with the highest yield potential consisted in the selection of the yield per se, which resulted in cultivars with higher grains per spike (GN) and occasionally increased grain weight (GW) (main numerical components of the yield). In this study, quantitative trait loci (QTL) for GW, GN and spike fertility traits related to GN determination were mapped using two doubled haploid (DH) populations (Baguette Premium 11 × BioINTA 2002 and Baguette 19 × BioINTA 2002). Results In total 305 QTL were identified for 14 traits, out of which 12 QTL were identified in more than three environments and explained more than 10% of the phenotypic variation in at least one environment. Eight hotspot regions were detected on chromosomes 1A, 2B, 3A, 5A, 5B, 7A and 7B in which at least two major and stable QTL sheared confidence intervals. QTL on two of these regions (R5A.1 and R5A.2) have previously been described, but the other six regions are novel. Conclusions Based on the pleiotropic analysis within a robust physiological model we conclude that two hotspot genomic regions (R5A.1 and R5A.2) together with the QGW.perg-6B are of high relevance to be used in marker assisted selection in order to improve the spike yield potential. All the QTL identified for the spike related traits are the first step to search for their candidate genes, which will allow their better manipulation in the future.

scholarly journals Chromosomal Map of the Model LegumeLotus japonicus

Genetics ◽  
2002 ◽  
Vol 161 (4) ◽  
pp. 1661-1672 ◽  
Author(s):  
Andrea Pedrosa ◽  
Niels Sandal ◽  
Jens Stougaard ◽  
Dieter Schweizer ◽  
Andreas Bachmair
Keyword(s):  
Repetitive Sequences ◽  
Lotus Japonicus ◽  
Chromosome Complement ◽  
Linkage Groups ◽  
Closely Related Species ◽  
F1 Hybrid ◽  
Bac Clones ◽  
Genomic Regions ◽  
Chromosomal Map

AbstractLotus japonicus is a model plant for the legume family. To facilitate map-based cloning approaches and genome analysis, we performed an extensive characterization of the chromosome complement of the species. A detailed karyotype of L. japonicus Gifu was built and plasmid and BAC clones, corresponding to genetically mapped markers (see the accompanying article by Sandal  et al. 2002, this issue), were used for FISH to correlate genetic and chromosomal maps. Hybridization of DNA clones from 32 different genomic regions enabled the assignment of linkage groups to chromosomes, the comparison between genetic and physical distances throughout the genome, and the partial characterization of different repetitive sequences, including telomeric and centromeric repeats. Additional analysis of L. filicaulis and its F1 hybrid with L. japonicus demonstrated the occurrence of inversions between these closely related species, suggesting that these chromosome rearrangements are early events in speciation of this group.

scholarly journals QTL analysis of seed germination traits in tobacco (Nicotiana tabacum L.)

2021 ◽  
Author(s):  
Monika Agacka-Mołdoch ◽  
Mian Abdur Rehman Arif ◽  
Ulrike Lohwasser ◽  
Teresa Doroszewska ◽  
Ramsey S. Lewis ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Seed Germination ◽  
Doubled Haploid ◽  
Mapping Population ◽  
Area Under The Curve ◽  
Linkage Groups ◽  
Polygenic Control ◽  
Nicotiana Tabacum L ◽  
Controlled Deterioration ◽  
Germination Traits

AbstractGenetic mapping of seed germination traits has been performed with many plant species. In tobacco, however, investigations are rare. In the present study, a bi-parental mapping population consisting of 118 doubled haploid lines and derived from a cross between ‘Beinhart-1000’ and ‘Hicks’ was investigated. Four germination-related traits, total germination (TG), normal germination (NG), time to reach 50% of total germination (T50), and the area under the curve after 200 h of germination (AUC) were considered by examining seeds either untreated or after a moderate controlled deterioration (CD). Quantitative trait loci were found for all traits distributed on 11 out of the 24 linkage groups. It was demonstrated that, as in many other species, germination-related traits are very complex and under polygenic control.

Development of simple sequence repeat markers and construction of a high-density linkage map of Capsicum annuum

2013 ◽  
Vol 31 (4) ◽  
pp. 909-920 ◽  
Author(s):  
Toru Sugita ◽  
Yukari Semi ◽  
Hiromasa Sawada ◽  
Yumi Utoyama ◽  
Yuko Hosomi ◽  
...  
Keyword(s):  
Linkage Map ◽  
Capsicum Annuum ◽  
Simple Sequence Repeat ◽  
High Density ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
High Density Linkage Map ◽  
Simple Sequence

scholarly journals An enhanced molecular marker based genetic map of perennial ryegrass (Lolium perenne) reveals comparative relationships with other Poaceae genomes

Genome ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 282-295 ◽  
Author(s):  
Elizabeth S Jones ◽  
Natalia L Mahoney ◽  
Michael D Hayward ◽  
Ian P Armstead ◽  
J Gilbert Jones ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Linkage Map ◽  
Genetic Map ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Genetic Linkage Map ◽  
Population Based ◽  
Genetic Maps ◽  
Linkage Groups ◽  
Integrated Genetic Map

A molecular-marker linkage map has been constructed for perennial ryegrass (Lolium perenne L.) using a one-way pseudo-testcross population based on the mating of a multiple heterozygous individual with a doubled haploid genotype. RFLP, AFLP, isoenzyme, and EST data from four collaborating laboratories within the International Lolium Genome Initiative were combined to produce an integrated genetic map containing 240 loci covering 811 cM on seven linkage groups. The map contained 124 codominant markers, of which 109 were heterologous anchor RFLP probes from wheat, barley, oat, and rice, allowing comparative relationships between perennial ryegrass and other Poaceae species to be inferred. The genetic maps of perennial ryegrass and the Triticeae cereals are highly conserved in terms of synteny and colinearity. This observation was supported by the general agreement of the syntenic relationships between perennial ryegrass, oat, and rice and those between the Triticeae and these species. A lower level of synteny and colinearity was observed between perennial ryegrass and oat compared with the Triticeae, despite the closer taxonomic affinity between these species. It is proposed that the linkage groups of perennial ryegrass be numbered in accordance with these syntenic relationships, to correspond to the homoeologous groups of the Triticeae cereals.Key words: Lolium perenne, genetic linkage map, RFLP, AFLP, conserved synteny.

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