The identification of random amplified polymorphic DNA markers for daylength insensitivity in oat

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 910-914 ◽  
Author(s):  
Charlene P. Wight ◽  
Greg A. Penner ◽  
Louise S. O'Donoughue ◽  
Vernon D. Burrows ◽  
Stephen J. Molnar ◽  
...  
Keyword(s):  
Avena Sativa ◽  
Dna Markers ◽  
Field Data ◽  
Bulk Segregant Analysis ◽  
Segregation Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Dominant Gene ◽  
Dominant Allele ◽  
Single Dominant Gene ◽  
Polymorphic Bands

Daylength insensitive accessions of Avena sativa L. are being used to develop cultivars that will flower normally when grown under short or long photoperiods. Field data indicate that the insensitivity trait is under the control of a single dominant gene, designated Di1. The random amplified polymorphic DNA (RAPD) technique and bulk segregant analysis of daylength sensitive and insensitive plants were used to find markers for this gene. Five of 200 random decamer primers tested produced polymorphic bands, which were shown to be linked to the trait using 30 homozygous insensitive and 30 homozygous sensitive F3 individuals. Three of the markers produced a band in the presence of the dominant allele, and two in its absence. Segregation analysis showed that markers 221 and 136 could be mapped to within 9.8 ± 4.6 and 13.9 ± 5.4 cM of the trait, respectively; that is, close enough to be useful in a breeding program. A study of different cultivars suggested that the band produced by primer 136 is actually the more closely linked marker and the only one present in the original Di1 gene donor CAV2700. The possibility of using both markers in populations derived from different cultivars is discussed.Key words: RAPD, molecular marker, oat, daylength insensitivity, photoperiod, bulk segregant analysis.

Genetic analysis and molecular markers associated with multi-gynoecia (Mg) gene in Trigrain whea

2009 ◽  
Vol 89 (5) ◽  
pp. 845-850 ◽  
Author(s):  
Z Wang ◽  
D Xu ◽  
J Ji ◽  
J Wang ◽  
M Wang ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Simple Sequence Repeats ◽  
Segregation Analysis ◽  
Dominant Gene ◽  
Distal Region ◽  
Allelism Test ◽  
Single Dominant Gene ◽  
Gene Segregation ◽  
Genetic Patterns ◽  
Simple Sequence

Trigrain wheat normally produces up to three gynoecia in a single floret and forms three close-set grains. The gene conferring the multi grain phenotype was earlier designated Mg, the multiple gynoecia gene. Different genetic patterns controlling this trait have been reported. In the present work we studied the inheritance of the three grains trait and identified simple sequence repeats (SSR) markers linked to the Mg gene. Segregation analysis in the cross IGDB-TW (trigrain wheat)/Chinese Spring confirmed that a single dominant gene controlled the three grains trait. An allelism test showed that the same gene controlled the trigrain trait in line Trigrain-Yin 1. A total of 339 microsatellite markers were tested for polymorphism by bulked segregation analysis (BSA) in an F2 population. Six microsatellite markers, Xcfd233, Xgdm6, Xgdm87, Xgwm311, Xgwm349 and Xgwm539, on chromosome 2DL, were linked to Mg. Using the CS 2D deletion lines, Mg gene was localized to the distal region of chromosome 2DL. The microsatellite markers identified in this study have the potential for further mapping and map-based cloning of the gene.Key words: Simple sequence repeats, physical mapping, trigrain wheat

scholarly journals Identification of Co-1 Anthracnose Resistance and Linked Molecular Markers in Common Bean Line A193

Plant Disease ◽  
2001 ◽  
Vol 85 (3) ◽  
pp. 252-255 ◽  
Author(s):  
Azucena Mendoza ◽  
Fernando Hernández ◽  
Sanjuana Hernández ◽  
Daniel Ruíz ◽  
Octavio Martínez de la Vega ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Marker Assisted Selection ◽  
Fragment Length Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Dominant Gene ◽  
Colletotrichum Lindemuthianum ◽  
Single Dominant Gene ◽  
Selection For ◽  
Race 2

Phaseolus vulgaris line A193 has been shown to be widely resistant to Colletotrichum lindemuthianum, including race 1472, one of the most virulent races of C. lindemuthianum characterized. Resistance to C. lindemuthianum race 1472 in P. vulgaris line A193 was investigated in segregating F2 and F2.3 populations from a cross between A193 and Flor de Mayo Bajio (a commercial cultivar highly susceptible to C. lindemuthianum). Resistance to 1472 in A193 was determined to be conditioned by a single dominant gene. Inoculation of crosses between A193 and cultivars Michigan Dark Red Kidney and Perry Marrow with race 1472 suggest that resistance in A193 is conditioned by the Co-1 gene. Inoculation of the cross A193 × Perry Marrow with C. lindemuthianum race 2, demonstrated that resistance to race 2 in Perry Marrow is also conditioned by a single dominant gene and is distinct to resistance in A193 or Michigan Dark Red Kidney. Three AFLP markers (ECAG/MACC-1, EACA/MAGA-2, EAGG/MAAC-8) linked in repulsion to the Co-1 locus were identified by screening the A193 × Flor de Mayo F2 population with 314 random amplified polymorphic DNA, amplified fragment length polymorphism, and restriction fragment length polymorphism markers. The two most closely linked markers should be useful in marker-assisted selection for Co-1.

scholarly journals Mapping of resistance genes to races 1, 3 and 5 of Podosphaera xanthii in melon PI 414723

2013 ◽  
Vol 13 (4) ◽  
pp. 349-355 ◽  
Author(s):  
Ana Carolina Fazza ◽  
Leandro José Dallagnol ◽  
Ana Cristina Fazza ◽  
Carolina C. Monteiro ◽  
Bruno Marco de Lima ◽  
...  
Keyword(s):  
Linkage Group ◽  
Genetic Map ◽  
Resistance Genes ◽  
Segregation Analysis ◽  
Specific Resistance ◽  
Dominant Gene ◽  
Inheritance Of Resistance ◽  
Podosphaera Xanthii ◽  
Single Dominant Gene ◽  
First Time

The fungus Podosphaera xanthii affects melon crops and presents several races controlled by race-specific resistance genes. The accession PI 414723 is resistant to races 1, 3 and 5 and it is a suitable source of resistance genes. The inheritance of resistance to these races was analyzed on 87 F2 plants from the cross of PI 414723 × Védrantais, and resistance to all three races could be explained by the segregation of a single dominant gene, although a digenic model could also be accepted. A genetic map was assembled with 206 markers, and co-segregation analysis of resistance phenotypes indicated the existence of two linked loci in linkage group II, one conferring resistance to races 1 and 5 (denominated Pm-x1,5), and the second to race 3 (denominated Pm-x3), located 5.1 cM apart. This study reports for the first time the existence of Pm-x3 and the genetic locations of these resistance genes from PI 414723.

scholarly journals A Sequence-amplified Characterized Region Marker for a Single, Dominant Gene in Melon PI 134198 that Confers Resistance to a Unique Race of Podosphaera xanthii in China

HortScience ◽  
2010 ◽  
Vol 45 (9) ◽  
pp. 1407-1410 ◽  
Author(s):  
Longzhou Liu ◽  
Youyuan Chen ◽  
Zhenghong Su ◽  
Hui Zhang ◽  
Weiming Zhu
Keyword(s):  
Marker Assisted Selection ◽  
Bulk Segregant Analysis ◽  
Dominant Gene ◽  
Srap Marker ◽  
Podosphaera Xanthii ◽  
Single Dominant Gene ◽  
Yield Losses ◽  
Breeding Programs ◽  
Cucurbit Powdery Mildew ◽  
Selection Of

Melon production in China is threatened by cucurbit powdery mildew incited by Podosphaera xanthii, which can cause severe yield losses. Disease reactions of 10 melon P. xanthii race differentials indicated the presence of a unique race of P. xanthii, denoted pxCh1, in the Shanghai area. Resistance to P. xanthii race pxCh1 in melon PI 134198, which originated from India, was controlled by a single, dominant gene, designated Pm-8, in a cross with susceptible melon inbred S717. A search for a molecular marker linked to Pm-8 was initiated using the same cross. Bulk segregant analysis of their F2 progeny revealed one sequence-related amplified polymorphism (SRAP) marker of 148 bp linked to Pm-8, at 3.9 cM. The SRAP segment was recovered, cloned, and sequenced and converted into a sequence-amplified characterized region marker for marker-assisted selection of Pm-8 in breeding programs to develop melon cultivars resistant to P. xanthii race pxCh1.

scholarly journals Genetic and Molecular Analyses in Crosses of Race 2 and Race 7 of Albugo candida

2003 ◽  
Vol 93 (8) ◽  
pp. 959-965 ◽  
Author(s):  
Tika B. Adhikari ◽  
Jean Q. Liu ◽  
Snehlata Mathur ◽  
Chunren X. Wu ◽  
S. Roger Rimmer
Keyword(s):  
Dna Markers ◽  
Fragment Length Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Dominant Gene ◽  
Avirulence Gene ◽  
Susceptible Host ◽  
Albugo Candida ◽  
White Rust ◽  
Differential Cultivars ◽  
Race 2

The inheritance of avirulence and polymorphic molecular markers in Albugo candida, the cause of white rust of crucifers, was studied in crosses of race 2 (Ac2), using isolates MiAc2-B1 or MiAc2-B5 (metalaxyl-insensitive and virulent to Brassica juncea cv. Burgonde) with race 7 (Ac7), using isolate MsAc7-A1 (metalaxyl-sensitive and virulent to B. rapa cv. Torch). Hybrids were obtained via co-inoculation onto a common susceptible host. Putative F1 progeny were selfed to produce F2 progeny. The parents and F1 progeny were examined for virulence on the differential cultivars B. juncea cv. Burgonde and B. rapa cv. Torch. Segregation of avirulence or virulence of F2 populations was analyzed on cv. Torch. Putative F1 hybrids were confirmed by random amplified polymorphic DNA markers specific for each parent. Avirulence or virulence of F 2 progeny to B. rapa cv. Torch suggested 3:1 in each of three populations, supporting the hypothesis of a single dominant avirulence gene. Amplified fragment length polymorphism markers also segregated in regular Mendelian fashion among F2 progeny derived from two F1 hybrids (Cr2-5 and Cr2-7) of Cross-2. This first putative avirulence gene in A. candida was designated AvrAc1. These results suggest that a single dominant gene controls avirulence in race Ac2 to B. rapa cv. Torch and provides further evidence for the gene-for-gene relationship in the Albugo-Brassica pathosystem.

scholarly journals Identification and Characterization of Random Amplified Polymorphic DNA Markers Linked to a Major Gene (Cr2) for Resistance to Cronartium ribicola in Pinus monticola

2006 ◽  
Vol 96 (4) ◽  
pp. 395-399 ◽  
Author(s):  
Jun-Jun Liu ◽  
Abul K. M. Ekramoddoullah ◽  
Rich S. Hunt ◽  
Arezoo Zamani
Keyword(s):  
Dna Markers ◽  
Positional Cloning ◽  
Rapd Markers ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Dominant Gene ◽  
Cronartium Ribicola ◽  
R Genes ◽  
Pinus Monticola ◽  
White Pine

DNA markers tightly linked to resistance (R) genes provide a very powerful tool for both marker-assisted selection in plant breeding and positional cloning of R genes. In the present study, a linkage of random amplified polymorphic DNA (RAPD) markers to the single dominant gene (Cr2) for resistance to white pine blister rust fungus (Cronartium ribicola) was investigated in western white pine (Pinus monticola). A mapping population of 128 individual megagametophytes was generated from seeds of a heterozygous resistant tree (Cr2/cr2), and the corresponding seedlings of each megagametophyte were subjected to the test of phenotype segregation by inoculation with C. ribicola. Bulked segregant analysis and haploid segregation analysis identified eight robust RAPD markers linked to Cr2. This constitutes the first Cr2 genetic linkage map spanning 84.7 cM with four markers only 3.2 cM from Cr2. One sequence (U256-1385) of these linked markers was significantly similar to the Ty3/gypsy-like long terminal direct repeats retrotransposons. Another marker, U570-843, had no significant similarity to any entry in either GenBank or the loblolly genomics data bank. As presumed that the average physical distance per centimorgan is about 10 Mb in P. monticola, it is probably unrealistic to use these DNA markers for positional cloning of the Cr2 gene.

scholarly journals Identification of Markers Linked to Seedlessness in Citrus kinokuni hort. ex Tanaka and Its Progeny Using Bulked Segregant Analysis

HortScience ◽  
2011 ◽  
Vol 46 (5) ◽  
pp. 693-697 ◽  
Author(s):  
Dario J. Chavez ◽  
José X. Chaparro
Keyword(s):  
Molecular Markers ◽  
Linkage Group ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Dominant Gene ◽  
Segregation Ratio ◽  
Single Dominant Gene ◽  
Citrus Breeding

Citrus kinokuni ‘Mukaku kishu’ PI539530 and its progeny were studied to identify random amplified polymorphic DNA (RAPD) primers associated with seedlessness. Ninety-one F1 [(Robinson op) × C. kinokuni] individuals showed a 1:1 segregation ratio between seedless and seeded phenotypes with seedless as a single dominant gene. Bulked segregant analysis was used to identify markers associated with the seedless locus. Eighteen RAPD primers were mapped into a partial linkage group (≈55.8 cM length) with four RAPD primers flanking the seedless locus: OPAI11-0.8 at 8.7 cM, OPAJ19-1.0 at 8.4 cM, OPM06r-0.85 at 4.3 cM, and OPAJ04r-0.6 at 6.4 cM. The identification of molecular markers linked to C. kinokuni Fs seedless locus constitutes an important and major tool for citrus breeding and selection.

Resistance to Lettuce Infectious Yellows Virus in Melon

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 534b-534
Author(s):  
James D. McCreight
Keyword(s):  
Cucumis Melo ◽  
Dominant Gene ◽  
Field Tests ◽  
Single Dominant Gene ◽  
Limited Data ◽  
Sweetpotato Whitefly ◽  
Sources Of Resistance ◽  
Lettuce Infectious Yellows Virus ◽  
Greenhouse Tests

Yellowing of melon (Cucumis melo L.) incited by lettuce infectious yellows virus (LIYV) reduces yield and fruit quality of infected plants. LIYV is transmitted only by the sweetpotato whitefly (Bemisia tabaci Genn.). Two naturally infected field tests indicated several potential sources of resistance to LIYV. PI 124112 and `Snake Melon' had mild symptoms in both field tests whereas PI 313970 was asymptomatic in the test in which it was included. In greenhouse tests using controlled inoculation, PI 313970 was asymptomatic, had negative ELISA assays for LIYV, and was negative for LIYV in serial transfers to Chenopodium. `Top Mark' and `PMR 5' were symptomatic, had positive ELISA assays for LIYV, and were positive for LIYV in serial transfers to Chenopodium in these greenhouse tests. Limited data indicate that resistance in PI 313970 is conditioned by a single, dominant gene.

scholarly journals Independent Resistant Reactions Expressed in Root and Tuber of Potato Breeding Lines with Introgressed Resistance to Meloidogyne chitwoodi

2009 ◽  
Vol 99 (9) ◽  
pp. 1085-1089 ◽  
Author(s):  
C. R. Brown ◽  
H. Mojtahedi ◽  
L.-H. Zhang ◽  
E. Riga
Keyword(s):  
Genetic Factors ◽  
Dominant Gene ◽  
Breeding Line ◽  
Single Dominant Gene ◽  
Solanum Bulbocastanum ◽  
Chromosome 11 ◽  
Meloidogyne Chitwoodi ◽  
Tuber Resistance ◽  
Advanced Backcross ◽  
Resistance Breaking

Resistance to Meloidogyne chitwoodi was introgressed from Solanum bulbocastanum into the cultivated gene pool of potato. A single dominant gene is responsible for resistance to race 1 reproduction on the root system. An additional form of resistance was discovered in certain advanced backcross clones. A BC5 clone, PA99N82-4, resisted invasion of tubers by available nematode juveniles whether supplied by weeds or challenged by several root resistance-breaking pathotypes. This tuber resistance is inherited as a single dominant gene and is linked to RMc1(blb). Because this gene has been mapped to chromosome 11, tuber resistance genetic factors are inferred to be on the same chromosome in coupling phase. Among 153 progeny derived from crosses with PA99N82-4, 42 recombinants, comprising both resistant root/susceptible tuber and susceptible root/resistant tubers, were found while other progeny were doubly resistant (like PA99N82-4) or doubly susceptible. Therefore, the existence of two linked genetic factors controlling independently expressed traits is confirmed. The combination of the two phenotypes is likely to be a sufficient level of resistance to avoid tuber damage from circumstances that provide exogenous juveniles proximal to the tubers in the soil. These factors are weed hosts of M. chitwoodi host races and pathotypes of M. chitwoodi that overcome RMc1(blb). Under field conditions, where a resistance-breaking pathotype of M. chitwoodi was present, tuber-resistant PA99N82-4 breeding line produced tubers which were commercially acceptable and not culled. A related breeding line, root resistant but tuber susceptible, and Russet Burbank were severely tuber damaged and commercially unacceptable.

Direct comparison of levels of genetic variation in tomato detected by a GACA-containing microsatellite probe and by random amplified polymorphic DNA

Genome ◽  
1994 ◽  
Vol 37 (3) ◽  
pp. 375-381 ◽  
Author(s):  
W. Rus-Kortekaas ◽  
M. J. M. Smulders ◽  
P. Arens ◽  
B. Vosman
Keyword(s):  
Tissue Culture ◽  
Genetic Variation ◽  
Somaclonal Variation ◽  
Random Amplified Polymorphic Dna ◽  
Dna Fingerprint ◽  
Pairwise Comparisons ◽  
Lycopersicon Pennellii ◽  
Lycopersicon Species ◽  
Polymorphic Bands ◽  
Simple Sequence

In this study, a direct comparison was made of the ability of four selected random amplified polymorphic DNA (RAPD) primers and a GACA-containing microsatellite probe to detect genetic variation in Lycopersicon. Of the 89 RAPD primers initially tested, 85 showed differences between a representative of Lycopersicon pennellii and L. esculentum, but only 4 distinguished among three L. esculentum cultivars. These four primers were subsequently tested on representatives of six Lycopersicon species. In pairwise comparisons of species, all or 14 of the 15 combinations could be distinguished by single primers. When the primers were tested on 15 L. esculentum cultivars, 90 of the 105 combinations could be distinguished by the four primers together. Finally, none of 118 tested primers showed reproducible differences among calli or progeny of régénérants from tissue culture, although some of the plants had inherited morphological mutations. The probe pWVA16, which detects GACA-containing microsatellites, could distinguish in TaqI-digested DNA the representatives of Lycopersicon species as well as all the L. esculentum cultivars tested. The probe was unable to detect polymorphisms among calli and the progeny of regenerants from tissue culture. An analysis of the results showed that the four selected RAPD primers were able to detect polymorphic bands among species at a frequency of 80%, and among cultivars at a frequency of 44%. In contrast, the microsatellite probe detected polymorphic bands at a frequency of 100 and 95%, respectively. The GACA-containing probe did not detect any common bands among the representatives of the six species, while band sharing with RAPDs was 48%. These results indicate that the two methods detect two types of DNA that differ in their degree of variability.Key words: DNA fingerprint, RAPD, simple sequence, somaclonal variation, tissue culture.

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