Genetic variability and segregation analysis in Glossina morsitans morsitans (Diptera: Glossinidae) using DNA fingerprinting

Genome ◽  
1994 ◽  
Vol 37 (2) ◽  
pp. 289-295 ◽  
Author(s):  
A. Blanchetot ◽  
R. H. Gooding
Keyword(s):  
Genetic Variability ◽  
Dna Hybridization ◽  
Segregation Analysis ◽  
Marker Gene ◽  
Glossina Morsitans Morsitans ◽  
Inbred Lines ◽  
Tsetse Fly ◽  
Glossina Morsitans ◽  
Band Frequency ◽  
The Mean

DNA hybridization, using the M13 sequence as a probe, was used to analyze the genetic variability in four inbred lines of the tsetse fly Glossina morsitans morsitans Westwood. An average of 11.2 bands (ranging from 2 to 10 kb) were found per fly. An average of nine loci were detected in each line; 40% of the loci were polymorphic and the mean heterozygosity per locus varied from 0.098 to 0.29. Averaging the data across the four inbred lines, the band sharing estimates were 82.5% in males and 81.2% in females, and the mean band frequency estimates were 0.71 and 0.70 for males and females, respectively. Segregation of fragments, determined in "three generation" pedigrees, conformed to expected Mendelian ratios and two of seven fragments studied were linked to an X chromosome marker gene, ocra (body color).Key words: Glossina morsitans morsitans, DNA hybridization, genetic variability, segregation analysis.

Genetic relationships between subspecies of the tsetse fly Glossina morsitans inferred from variation in mitochondrial DNA sequences

1984 ◽  
Vol 26 (6) ◽  
pp. 692-697 ◽  
Author(s):  
Martin Trick ◽  
Gabriel A. Dover
Keyword(s):  
Mitochondrial Dna ◽  
Dna Sequences ◽  
Dna Hybridization ◽  
Genetic Relationships ◽  
Glossina Morsitans Morsitans ◽  
Tsetse Fly ◽  
Tsetse Flies ◽  
Glossina Morsitans ◽  
Site Variation ◽  
Local Restriction

A 750 base pair segment of DNA from the tsetse fly Glossina morsitans morsitans was isolated by means of molecular cloning. It was shown by DNA hybridization to have substantial sequence homology with a defined region of the mitochondrial genomes of several Drosophila species. When used as a probe against DNA prepared from single tsetse flies, the cloned sequence revealed local restriction site variation between members of the G. morsitans subspecies complex. This feature was used to demonstrate maternal inheritance of the sequence in progeny of hybrid crosses and to assemble comparative restriction maps for a 3-kilobase segment of each mitochondrial genome. The data obtained from these exercises point to a higher genetic identity between G. m. morsitans and G. m. centralis than between either form and G. m. submorsitans.Key words: mitochondrial DNA, tsetse fly species, Glossina morsitans.

scholarly journals IDENTITY COEFFICIENTS IN FINITE POPULATIONS. I. EVOLUTION OF IDENTITY COEFFICIENTS IN A RANDOM MATING DIPLOID DIOECIOUS POPULATION

Genetics ◽  
1977 ◽  
Vol 86 (3) ◽  
pp. 697-713
Author(s):  
C Chevalet ◽  
M Gillois ◽  
R F Nassar
Keyword(s):  
Genetic Variability ◽  
Population Size ◽  
Effective Population Size ◽  
Random Mating ◽  
Matrix Multiplication ◽  
Inbred Lines ◽  
Inbreeding Coefficient ◽  
Effective Population ◽  
The Mean ◽  
Over Time

ABSTRACT Properties of identity relation between genes are discussed, and a derivation of recurrent equations of identity coefficients in a random mating, diploid dioecious population is presented. Computations are run by repeated matrix multiplication. Results show that for effective population size (Ne) larger than 16 and no mutation, a given identity coefficient at any time t can be expressed approximately as a function of (1—f), (1—f)3 and (1—f)6, where f is the mean inbreeding coefficient at time t. Tables are presented, for small Ne values and extreme sex ratios, showing the pattern of change in the identity coefficients over time. The pattern of evolution of identity coefficients is also presented and discussed with respect to N eu, where u is the mutation rate. Applications of these results to the evolution of genetic variability within and between inbred lines are discussed.

scholarly journals Host-seeking efficiency can explain population dynamics of the tsetse fly Glossina morsitans morsitans in response to host density decline

2017 ◽  
Vol 11 (7) ◽  
pp. e0005730 ◽  
Author(s):  
Jennifer S. Lord ◽  
Zinhle Mthombothi ◽  
Vitalis K. Lagat ◽  
Fatumah Atuhaire ◽  
John W. Hargrove
Keyword(s):  
Population Dynamics ◽  
Host Density ◽  
Glossina Morsitans Morsitans ◽  
Tsetse Fly ◽  
Glossina Morsitans ◽  
Host Seeking

scholarly journals Two Tsetse Fly Species, Glossina palpalis gambiensis and Glossina morsitans morsitans, Carry Genetically Distinct Populations of the Secondary Symbiont Sodalis glossinidius

2005 ◽  
Vol 71 (12) ◽  
pp. 8941-8943 ◽  
Author(s):  
Anne Geiger ◽  
Gérard Cuny ◽  
Roger Frutos
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Glossina Morsitans Morsitans ◽  
Tsetse Fly ◽  
Glossina Morsitans ◽  
Glossina Palpalis Gambiensis ◽  
Sodalis Glossinidius ◽  
Glossina Palpalis

ABSTRACT Genetic diversity among Sodalis glossinidius populations was investigated using amplified fragment length polymorphism markers. Strains collected from Glossina palpalis gambiensis and Glossina morsitans morsitans flies group into separate clusters, being differentially structured. This differential structuring may reflect different host-related selection pressures and may be related to the different vector competences of Glossina spp.

Sterilising effects of benzyl-l,3-benzodioxoles on the tsetse fly Glossina morsitans morsitans Westwood (Diptera: Glossinidae)

1982 ◽  
Vol 72 (3) ◽  
pp. 473-481 ◽  
Author(s):  
P. A Langley ◽  
M. A Trewern ◽  
L. Jurd
Keyword(s):  
Topical Application ◽  
Glossina Morsitans Morsitans ◽  
Mature Oocyte ◽  
Tsetse Fly ◽  
Glossina Morsitans ◽  
Plant Materials ◽  
Female Survival ◽  
Low Solubility ◽  
Blood Meals ◽  
Contact Insecticides

AbstractThree benzyl-l,3-benzodioxoles and three benzylphenols were tested for their ability to sterilise Glossina morsitans morsitans Westw. following topical application in acetone. Two of the former compounds, 5-ethoxy-6- (4-methoxyphenylmethyl)-l,3-benzodioxole and 5-ethoxy-6-[l-(4-meth-oxyphenyl)ethyl]-l,3-benzodioxole, caused total sterility in females at a dose of 10 μg per fly, and remained effective even after one year's storage at 4°C. Dose-fecundity data indicate a reduction to 50% fecundity (ED50) was achieved with 2·5 μg of the former or 0·5 μg of the latter per fly. An ED90 of 8·5 μg and 2·5 μg, respectively, is indicated. The latter compound reduced the survival of treated females and males and surviving males were incapable of inseminating even though copulation appeared normal. The effects on female survival and fecundity, of feeding the compounds at 0·25 and 1·0% in blood, were variable, owing to their low solubility and variations in the size of blood-meals. The highest doses of the former compound led to ovarian atrophy in females, which can be described as an effect on fecundity. Lower doses induced sterility through egg death and atrophy after ovulation. The latter compound acted more rapidly than the former, preventing ovulation of the first mature oocyte in a higher proportion of flies. The activity of these compounds, which were derived by modification of plant materials, and are non-mutagenic, renders them likely candidates for evaluation as contact insecticides for Glossina in the field.

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