Mapping quantitative powdery mildew resistance of barley using a restriction fragment length polymorphism map

Genome ◽  
1992 ◽  
Vol 35 (6) ◽  
pp. 1019-1025 ◽  
Author(s):  
M. Heun
Keyword(s):  
Quantitative Trait Loci ◽  
Powdery Mildew ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Quantitative Trait ◽  
Fragment Length Polymorphism ◽  
Trait Loci ◽  
Restriction Fragment Length

To map the genes underlying the quantitative resistance (QR) of the spring barley cultivars Proctor and Nudinka to powdery mildew, the relative pustule number of F1-derived doubled haploids (DHs) was determined and combined with the restriction fragment length polymorphism (RFLP) mapping data of these DHs via MAPMAKER/QTL. Applying a log-likelihood (LOD) threshold of 2.50, two putative quantitative trait loci (QTLs) were found in two different genome areas that accounted for 19.8% of the observed variance. One of the QR-related RFLPs (Xcnl.CDO36) is located on chromosome 1S, the other one (Xcnl.CDO749) is tightly linked with the nucleolus organizer region on chromosome 7. By comparing the means of subpopulations of DHs carrying alternative parental marker alleles, it was found that both RFLPs were also linked with some genes causing thousand kernel weight differences in this population. For the QR trait measured, LOD scores < 1.00 were found for the Mla12 region on chromosome 5S indicating that the Mla12 virulent isolate used neutralized this major resistance gene and that no residual effect of that locus occurred.Key words: barley, powdery mildew, restriction fragment length polymorphism, quantitative trait loci.

QTL analysis of morphological traits in a tomato recombinant inbred line population

Genome ◽  
1997 ◽  
Vol 40 (2) ◽  
pp. 242-248 ◽  
Author(s):  
I. Paran ◽  
I. Goldman ◽  
D. Zamir
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Quantitative Trait ◽  
Recombinant Inbred ◽  
Morphological Traits ◽  
Fragment Length Polymorphism ◽  
Recombinant Inbred Lines ◽  
Restriction Fragment Length

Quantitative trait loci (QTLs) influencing morphological traits were identified by restriction fragment length polymorphism (RFLP) analysis in a population of recombinant inbred lines (RILs) derived from a cross of the cultivated tomato Lycopersicon esculentum with a related wild species, Lycopersicon cheesmanii. One hundred and thirty-two RFLP loci spaced throughout the tomato genome were used as DNA probes on genomic DNA from 97 RIL families. Morphological traits, including plant height, plant fresh mass, number of branches, number of nodes, first flower-bearing node, and leaf length, were evaluated in two controlled environment trials in 1992 and 1993. QTLs were detected via regression analyses at multiple marker loci for each morphological trait. A total of 41 markers were significantly associated with the traits examined. Large additive effects were measured at many of these loci. QTLs for multiple traits were detected on chromosomes 3 (TG74) and 4 (CT188), suggesting the possible association of these chromosome segments with genes controlling growth and development in tomato. These chromosomal regions were also associated with multiple morphological traits in a L. esculentum × Lycopersicon pennellii cross. A total of 13% of the QTLs identified for traits common to both studies occupied similar map positions.Key words: Lycopersicon, quantitative trait, restriction fragment length polymorphism.

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