Chromosomes and DNA of Anguilla anguilla: a study with restriction endonucleases

Genome ◽  
1992 ◽  
Vol 35 (5) ◽  
pp. 838-843 ◽  
Author(s):  
A. Cau ◽  
E. Coluccia ◽  
A. M. Deiana ◽  
G. Pichiri ◽  
R. Rossino ◽  
...  
Keyword(s):  
Sex Chromosomes ◽  
Anguilla Anguilla ◽  
Restriction Enzymes ◽  
Restriction Endonucleases ◽  
Repetitive Dnas ◽  
Fish Chromosomes ◽  
Dna Restriction ◽  
Dna Restriction Enzymes

We have studied fixed chromosomes and purified DNA of Anguilla anguilla L. after digestion with HaeIII, AluI, MboI, and DdeI restriction endonucleases. Our data demonstrated (i) confirmation of the heteromorphic nature of NORs, (ii) absence of detectable sex chromosomes, and (iii) presence of discrete intercalary domains in this species. Our data also permitted us to hypothesize the existence of highly repetitive DNAs, localized in specific heterochromatic regions of A. anguilla chromosomes.Key words: fish, chromosomes, DNA, restriction enzymes.

DNA mimicry by proteins

2006 ◽  
Vol 34 (2) ◽  
pp. 317-319 ◽  
Author(s):  
D.T.F. Dryden ◽  
M.R. Tock
Keyword(s):  
Double Helix ◽  
Restriction Enzymes ◽  
Repair Enzymes ◽  
Phosphate Backbone ◽  
Dna Mimicry ◽  
Associated Proteins ◽  
Bonding Properties ◽  
Dna Restriction ◽  
Dna Double Helix ◽  
Dna Restriction Enzymes

It has been discovered recently, via structural and biophysical analyses, that proteins can mimic DNA structures in order to inhibit proteins that would normally bind to DNA. Mimicry of the phosphate backbone of DNA, the hydrogen-bonding properties of the nucleotide bases and the bending and twisting of the DNA double helix are all present in the mimics discovered to date. These mimics target a range of proteins and enzymes such as DNA restriction enzymes, DNA repair enzymes, DNA gyrase and nucleosomal and nucleoid-associated proteins. The unusual properties of these protein DNA mimics may provide a foundation for the design of targeted inhibitors of DNA-binding proteins.

On the structure and operation of type I DNA restriction enzymes

1999 ◽  
Vol 290 (2) ◽  
pp. 565-579 ◽  
Author(s):  
Graham P Davies ◽  
Ina Martin ◽  
Shane S Sturrock ◽  
Andrew Cronshaw ◽  
Noreen E Murray ◽  
...  
Keyword(s):  
Restriction Enzymes ◽  
Type I ◽  
Dna Restriction ◽  
Dna Restriction Enzymes

scholarly journals Structure and operation of the DNA-translocating type I DNA restriction enzymes

2012 ◽  
Vol 26 (1) ◽  
pp. 92-104 ◽  
Author(s):  
C. K. Kennaway ◽  
J. E. Taylor ◽  
C. F. Song ◽  
W. Potrzebowski ◽  
W. Nicholson ◽  
...  
Keyword(s):  
Restriction Enzymes ◽  
Type I ◽  
Dna Restriction ◽  
Dna Restriction Enzymes

Induction of G-bands on Anguilla anguillachromosomes by the restriction endonucleases Haelll, HinfI, and MseI

1994 ◽  
Vol 65 (1-2) ◽  
pp. 79-81 ◽  
Author(s):  
A. Viñas ◽  
C. Gómez ◽  
P. Martinez ◽  
L. Sánchez
Keyword(s):  
Anguilla Anguilla ◽  
Restriction Endonucleases

Sexing beluga whales (Delphinapterus leucas) by means of DNA markers

1991 ◽  
Vol 69 (7) ◽  
pp. 1971-1976 ◽  
Author(s):  
Moira W. Brown ◽  
Ree Helbig ◽  
Peter T. Boag ◽  
David E. Gaskin ◽  
Bradley N. White
Keyword(s):  
Y Chromosome ◽  
Zinc Finger Protein ◽  
Restriction Enzymes ◽  
Delphinapterus Leucas ◽  
Gene Probe ◽  
Beluga Whales ◽  
Zinc Finger Protein Gene ◽  
Dna Restriction ◽  
Male Specific ◽  
Free Ranging

Few methods are available for determining the sex of free-ranging individual whales, dolphins, and porpoises of species that are not obviously sexually dimorphic. We have developed a technique for sexing beluga whales (Delphinapterus leucas) by using a Y-chromosome-specific DNA restriction fragment. Genomic DNA was extracted from liver samples of 18 beluga whales (9 males, 9 females) sexed at dissection. DNA from males and females was digested with five restriction enzymes, electrophoresed, and transferred to membranes by Southern blotting. When probed with the labelled human Y-chromosome zinc finger protein gene probe pDP1007, male-specific bands and bands common to both sexes, but more intense in females than in males, were observed. The DNA digested with EcoRI provided the clearest sex-discriminating banding pattern. Even when DNA of various qualities digested with EcoRI was used, all the males showed a 3.4-kilobase (kb) band, presumably from the Y-chromosome, as well as a 2.1-kb band. Females showed the 2.1-kb band, but all lacked the 3.4-kb band. This 3.4-kb EcoRI male-specific band permits unambiguous sex determination, which will facilitate examination of sex-related differences in population structure and habitat use of belugas, which have important implications for management decisions.

Mitochondrial DNA variation in Drosophila pseudoobscura and related species in Pacific Northwest populations

1985 ◽  
Vol 27 (3) ◽  
pp. 357-364 ◽  
Author(s):  
Lawrence R. Hale ◽  
Andrew T. Beckenbach
Keyword(s):  
Mitochondrial Dna ◽  
Pacific Northwest ◽  
Restriction Enzymes ◽  
The Other ◽  
Drosophila Pseudoobscura ◽  
Rich Region ◽  
Dna Variation ◽  
Restriction Sites ◽  
Dna Restriction ◽  
Tandem Duplications

We have analysed mitochondrial DNA (mtDNA) from Pacific Northwest populations of Drosophila pseudoobscura, D. persimilis, and D. miranda using six restriction enzymes. We find that HpaII restriction sites are hypervariable compared to the other enzymes used. This hypervariability allows construction of a maximum parsimony map linking each mtDNA genotype. Small insertions, possibly tandem duplications, appear to have arisen concomitantly with, or subsequent to, speciation events, perhaps within the A + T rich region. Convergence of mtDNA genotypes is also evident. Unlike findings for other populations of these species, we find little evidence of mitochondrial introgression between D. pseudoobscura and D. persimilis, despite their ability to produce fertile hybrid females.Key words: mitochondrial DNA, restriction endonucleases, Drosophila, evolution.

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