Application of chromosome-specific monoclonal antibodies in wheat genetics

Genome ◽  
1992 ◽  
Vol 35 (5) ◽  
pp. 831-837 ◽  
Author(s):  
R. E. Knox ◽  
N. K. Howes ◽  
T. Aung
Keyword(s):  
Monoclonal Antibodies ◽  
Gel Electrophoresis ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Polyacrylamide Gel ◽  
Root Tip ◽  
Chromosome Marker

A monoclonal antibody (P24B) to a wheat gliadin protein coded by a gene on the short arm of chromosome 1B was used as a chromosome marker. Somatic chromosome number for the 1B chromosome, as predicted by the level of binding of the antibody to extracts from seeds of single cross F2 and testcross F1 populations, was confirmed with sodium dodecyl sulfate – polyacrylamide gel electrophoresis and root-tip analysis. The implications of monoclonal antibodies as tools for cytogenetic analysis are discussed.Key words: polyacrylamide gel electrophoresis, aneuploid, cytogenetics, seed storage proteins.

Diversity of seed storage proteins in common wheat (Triticum aestivum L.)

2011 ◽  
Vol 9 (2) ◽  
pp. 256-259
Author(s):  
Zuzana Šramková ◽  
Edita Gregová ◽  
Svetlana Šliková ◽  
Ernest Šturdík
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Gel Electrophoresis ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Aestivum L ◽  
Polyacrylamide Gel

The objective of our study was to determine the composition of high-molecular weight-glutenin subunits (HMW-GS) in 120 cultivars of common wheat (Triticum aestivum L.). Fourteen alleles and 34 allelic compositions were detected using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The most frequent HMW-GS alleles at the Glu-A1, Glu-B1 and Glu-D1 loci were null (57.1%), 7+9 (43.3%) and 5+10 (61.9%), respectively. However, low-frequency HMW-GS alleles were also observed, such as 13+16, 20, 21, 7 and 18, encoded by the Glu-B1 locus, and 4+12, encoded by the Glu-D1 locus. The wheat–rye 1BL.1RS translocation was identified in 25 cultivars, using acid polyacrylamide gel electrophoresis. The Glu-score varied greatly, and some lines reached the maximum value of 10.

Revealing genetic diversity in land races and wild accessions of mungbean [Vigna radiata (L.) Wilczek] using SDS-PAGE of seed storage proteins

2015 ◽  
Author(s):  
Ananya Panda ◽  
Swapan K. Tripathy
Keyword(s):  
Storage Proteins ◽  
Seed Storage Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Land Races ◽  
Sds Page ◽  
Wild Accessions ◽  
Total Seed

Total seed storage protein profiles of 74 mungbean land races, three wild accessions and a popular variety ‘Jyoti’ of Odisha were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 32 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into eight protein types. Genotypes included in each protein type had 100% homology and some of these could be duplicates. In this pursuit, a few specific polypeptide markers have been detected for identification of the land races/ genotypes. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 70% phenon level. Paralakhemundi local, Samarjhola local and Phulbani local-D; and three wild accessions (TCR 20, TCR 213 and TCR 243) were comparatively divergent from other genotypes. Besides, Jyoti, Kalahandi local 2A, Sikri local, kodala local A and TCR 20 were identified to be protein rich with high seed yield. TCR 20 being morphologically similar to mungbean, moderately high protein content and high yielding as well as resistant to drought and bruchids; it may serve as a valuable source genotype in recombination breeding

scholarly journals THE USE OF SEED PROTEIN BANDING PATTERNS FOR IDENTIFICATION OF PASTURE CULTIVARS

1988 ◽  
pp. 87-91
Author(s):  
M.B. Forde ◽  
S.E. Gardiner
Keyword(s):  
Gel Electrophoresis ◽  
Seed Protein ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Subterranean Clover ◽  
Seed Storage Proteins ◽  
Polyacrylamide Gel ◽  
Banding Patterns ◽  
Seed Certification ◽  
Plant Variety

Because of the growing number of pasture cultivars used in NZ and the difficulty of reliably separating cultivars of the same species by morphological characters, seed protein banding patterns have become a useful supplementary means of cultivar identification for the purposes of seed certification and plant variety rights applications. Sodium dodecylsufphate polyacrylamide gel electrophoresis of proteins extracted from ground seed samples produces distinctive patterns of bands representing seed storage proteins of different molecular weights. The procedure can be carried out in two days using viable or dead seed, and the results are not affected by site and season of growth. Although individual seeds of outbreeding species such as perennial ryegrass and white clover produce different banding patterns, the combined population representing the cultivar remains constant unless there has been genetic shift during seed multiplication. Speckes for whrch this procedure is being successfully used include the ryegrasses and fescues, browntop, cocksfoot, bromes, red and white clovers, subterranean clover, serradella and lotus. Even cultrvars as closely related as Nui and Ellett ryegrasses and Huia and Pitau white clovers can be separated by careful work. Because of minor technical differences between runs, all cultivars to be compared must be run on the same gel. Keywords: Seed certification, Plant variety rights, sodium dodecylsulphate polyacrylamide gel electrophoresis.

Profiling the Seed Storage Protein Among Different Genotypes of Trigonella foenum-graecum L. (Fenugreek)

2019 ◽  
Author(s):  
Nisha . ◽  
Priyanka Khati ◽  
P B Rao
Keyword(s):  
Storage Protein ◽  
Gel Electrophoresis ◽  
Storage Proteins ◽  
Seed Storage Protein ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Protein Band ◽  
Trigonella Foenum Graecum ◽  
Trigonella Foenum Graecum L

A qualitative as well as quantitative categorization of seed storage proteins profiles of 23 genotypes of Trigonella foenum- graecum L. were performed by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) for exploring the level of genetic discrepancy at seed storage protein level. Total soluble proteins were resolved on 10% resolving gel. A dendrogram was constructed on the basis of weight of seed storage proteins, which divide total genotypes into two groups further classified into different sub groups containing different genotypes in them. The bands obtained from gel electrophoresis can serve as a potent tool in discrimination of different genotypes on the basis of their protein content. Proteins with molecular weight 66, 43 and 35 kDa were found in all the genotypes except Fgk-76, PR, Rmt-303, PEB and Rmt-361, The 43 kDa protein band was found missing in Fgk-67, AFg-2, AM-2, AFg-4, Fgk-73, although the protein with 35 kDa weight was present in all the genotypes but not in Rmt-303 same as 63 kDa which is not present in Fgk-70 and 55 kDa protein band was found missing in Fgk-67, Afg-4 and Rmt-361.

Genetic Effects on the Proportions of Conglutin γ and a Subunit of Conglutin β in the Storage Proteins of Lupinus angustifolius L. Seeds

1981 ◽  
Vol 8 (3) ◽  
pp. 277 ◽  
Author(s):  
RN Oram ◽  
JM Gillespie ◽  
RJ Blagrove
Keyword(s):  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Lupinus Angustifolius ◽  
Β Subunit ◽  
Partial Dominance ◽  
High Level ◽  
Conglutin Γ

The genetic variability in the proportions of conglutin β and γ components of lupin seed storage proteins was explored in F1, F2 and F3 families generated from a cross between two genotypes of Lupinus angustifolius cv. Uniharvest and a white-seeded selection from CPI 47644. The levels of conglutin γ differed by a factor of two in these parents, but varied continuously in the F2 and F3 families, indicating that the parental difference is due to several genes. These parents also differed in the level of a major conglutin β subunit, with a molecular weight of 30 000. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the storage globulins from individual F1, F2 and F3 seeds showed that the high level in cv. Uniharvest is controlled by an incompletely dominant allele, Bsc, at one locus, whereas the low level in CPI 47644 (white-seeded) is due to homozygosity for the recessive allele, bsc. The partial dominance of the Uniharvest gene suggests that the locus has a regulatory or processing function, so it has been designated beta subunit controller. F3 families differed significantly in the level of the conglutin β subunit in Bsc/- seeds, indicating that other loci with smaller effects also influence the proportion of this subunit. The proportion of the β subunit in any seed depends largely on its own genotype, and not on the genotype of the maternal plant. The proportion of conglutin γ in the storage proteins varied inversely with the proportion of the β subunit. The implications of these results for the genetic improvement of the biological value of lupin protein are discussed.

Identifikasi Daging Tikus Pada Produk Baso Dengan Metode Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

2018 ◽  
Vol 26 (2) ◽  
pp. 058
Author(s):  
Anna P. Roswiem ◽  
Triayu Septiani
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Sds Page

<em>Bahan<strong> </strong>baku untuk membuat baso adalah daging hewan, pada umumnya dari daging sapi, ayam, ikan dan babi. Di beberapa daerah di Indonesia terjadi kasus baso tikus. Tujuan penelitian ini adalah menguji ada tidaknya kandungan daging tikus pada produk baso yang dijual di pasar Cempaka Putih-Kecamatan Kramat Jakarta Pusat dan di pedagang baso atau mie baso di sekitar kampus Universitas YARSI Jakarta. Daging adalah protein salah satu metode untuk mengidentifikasi protein adalah metode Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE).<strong> </strong>Hasil penelitian menunjukkan bahwa dari 6 sampel baso terindikasi ada 2 sampel baso dengan nomor 1 dan 5 yang dibuat dari campuran daging sapi dan tikus; ada 1 sampel baso dengan nomor 6 yang terbuat dari daging tikus; dan 2 sampel baso dengan nomor 2 dan 3 yang terbuat dari campuran sapi  dan babi, dan hanya 1 sampel baso dengan nomor sampel 4 yang benar-benar terbuat dari daging sapi.</em>

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